Garuda - Garba Rujukan Digital

Article Per Year (5 Year)

p-Index From 2021 - 2026

P-Index

This Author published in this journals

All Journal Zuriat JURNAL TEKNOLOGI LINGKUNGAN BERITA BIOLOGI JURNAL BIOLOGI INDONESIA

Yuyu S Poerba

Unknown Affiliation

Author-ID : 342938

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science

Published : 7 Documents Claim Missing Document

Claim Missing Document

Articles

Title

PENGARUH MUTAGEN ETIL METAN SULFONAT (EMS) TERHADAP PERTUMBUHAN KULTUR IN VITRO ILES-ILES {Amorphophallus muelleri Blume) Poerba, Yuyu S; Leksonowati, Aryani; Martanti, Diyah
BERITA BIOLOGI Vol 9, No 4 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Amorphophallus muelleri Blume (Araceae) is one of 27 Amorphophallus species occur wild in Indonesia (Sumatera, Java, Floresand Timor). The species is valued for its glucoman content for use in food industry (heathy diet food), paper industry, pharmacyand cosmetics. The cultivation of A. muelleri is hampered by limited genetic quality of seed. The species is triploid (2n=3x=39),the seed is developed apomictically. and pollen production is low. The species is only propagated vegetatively. This may explainthat the species is difficult to breed conventionally and genetic variabillity in the exiting landaraces cultivars is rather limited.Induced mutation using ethyl methan sulfonate is one of techniques to increase genetic variation. The present research is aimed todetermine Lethal Dosage (LD) 50% and 75% of EMS and to study effects of EMS on growth of A, muelleri in vitro cultures for usein induced mutation program. Results of the experiment showed that LD-50 and LD-75 was observed at 0.875% EMS and 0.5%EMS. respectively. Number of shoot, and percentage of rooting culture were decreasing as EMS level concentration increases.

MIKROPROPAGASI TANAMAN ILES-ILES {Amorphophallus muelleri Blume) Imelda, Maria; Wulansari, Aida; Poerba, Yuyu S
BERITA BIOLOGI Vol 8, No 4 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

In Indonesia, iles-iles (Amorphophallus muelleri Blume) has not yet been cultivated intensively; their cultivation area is still limited. This species contains high glucomannan, which is useful as food diet, paper pulp, textile, paint, film-negative, celluloid and cosmetic industry. The cultivation of A. muelleri is hampered by limited genetic quality of plant.The species is triploid (2n=3x=39), the seed is developed apomictically, and pollen production is low.This may explain that the species is difficult to breed conventionally and genetic variability in the existing landraces cultivars is rather limited. Genetic variability of this plant is therefore can be achieve by induced mutation through tissue cultures for use in breeding program to develop better cultivars.Developing an efficient and effective micropropagation of the species is therefore important for use in the genetic improvement program.In other hands, the prospect for development and export of iles-iles is high since the demand from Japan alone has not been fulfilled. Propagation of iles-iles is generally done by splitting tubers, bulbils or leaf cuttings, but this method can not yield planting materials in large quantities within a relatively short time. In this research, young shoots which had just appeared from tubers were used as a source of explants. Sterilization of the explants was carried out in 0.05 % HgCl, solution for 20 min, rinsed several times with sterile distilled water and then cultured on Murashige and Skoog (MS) medium containing 0.1-0.2 mg/1 Thidiazuron (TDZ), 0.5-1.0 mg/1 Benzylaminopurine (BAP) and 0.5-1.0 mg/1 Kinetin (KIN) singly or in combination. Acclimatization of plantlets was done on 3 kinds of media namely (A), soil + compost, (B) soil + compost.+ cocopeat, and (C) soil + cocopeat. The results showed that the best medium is MS containing 0.2 mg/1 TDZ and 0,5 mg/1 BAP for in vitro shootbuds induction and proliferation of iles-iles, while MS without plant growth regulators is suitable for shoot growth and root formation and soil + compost + cocopeat for acclimatization of plantlets.

KERAGAMAN FENOTIPE RAPD Santalum album L. DIPULAU TIMOR BAGIAN TIMUR Poerba, Yuyu S; Wawo, Albert H; Yulita, KS
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Santalum album L. (sandalwood/cendana) is known as one of medicinal and aromatic tree species in Indonesia. The species is valued for its quality light wood timber and for its medicinal properties.The species has been overexploited and is considered as vulnerable plant species.The present study aimed to assess genetic diversity and to estimate genetic relationship among 58 accessions of plant germplasm collection using Random Amplified Polymorphic DNA (RAPD).Two RAPD primers generated 34 scorable bands with 97.06% of them were polymorphic. Clustering analysis was performed based on RAPD profiles using the UPGMA method.The range of genetic dissimilarity value among species was from 6% to 91%, while the range of genetic distance between populations was from 1.89% and 26.88%.These values showed that 5.album from Eastern part of Timor was genetically diverse populations.Within the 12 populations, there were 9 banding patterns recorded from primer OPA 16 and 12 banding patterns from primer OPB 12, suggesting that OPB 12 was more sensitive than that of OPA 16 to show variation within the sample used.

Co-Authors ,, Witjaksono ,, Witjaksono Diyah Martanti, Diyah F. AHMAD IMELDA, MARIA Imelda, Maria Leksonowati, Aryani Tri Handayani Wawo, Albert H Wulansari, Aida Wulansari, Aida Yulita, KS

Title Search

Found 3 Documents Search Journal : BERITA BIOLOGI

Abstract

Abstract

Abstract

Title

Found 3 Documents
Search
Journal : BERITA BIOLOGI