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All Journal ACTA VETERINARIA INDONESIANA Vitek : Bidang Kedokteran Hewan Ovozoa: Journal of Animal Reproduction
ady kurnianto
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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DETEKSI BRUCELLOSIS PADA SAPI PERAH PERANAKAN FRIESIAN HOLSTEIN DI KECAMATAN PAGU KABUPATEN KEDIRI ady kurnianto; Bagus Uda Palgunadi; Roeswandono Roeswandono; Adilla Luthfita Fa’za
VITEK : Bidang Kedokteran Hewan Vol 9 (2019): VITEK - Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v9i0.64

Abstract

Brucellosis (Keluron disease) is a strategic infectious disease with zoonotic potential, one of which is genus Brucella abortus. This research aims to detect Brucellosis in dairy cattle FH in Pagu District, Kediri Regency. This research uses two serological tests, namely the Rose Bengal Test (RBT) and the Complement Fixation Test (CFT) with descriptive analysis method. 30 cattle blood samples were taken from the coccigea vein. The blood was set aside at room temperature to form a serum. The serum that has been obtained is taken to the Department of Food and Livestock of Kediri Regency for checking the RBT. The remaining serum obtained was sent to BBVet Wates for CFT examination. The results obtained show negative results on both tests. But one of the samples with the code S9 had gone through an abortion. This might occur due to other factors such as genetic disorders, the presence of viruses such as BVD, toxic agents and live vaccines during pregnancy.
Isolation and Molecular Identification of Nitrate-Reducing Bacteria from Swiftlet Houses in Sumedang, West Java Ningrum, Siti Gusti; Novik Nurhidayat; Titin Yulinery; Evi Triana; Olan Rahayu Puji Astuti Nussa; Ady Kurnianto
Acta VETERINARIA Indonesiana Vol. 13 No. 1 (2025): Maret 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.1.1-7

Abstract

Edible bird‘s nest is an important export commodity that is currently of concern to the Indonesian government. One of the requirements for exporting edible bird’s nest to the People's Republic of China is that this product must meet the requirements for nitrite content in the product below 30 ppm. This nitrite contamination can be obtained from the results of nitrogen metabolism from nitrifying bacteria. However, information on nitrite-reducing bacteria in swiftlet houses has never been reported and is urgent in controlling nitrifying bacteria in swiftlet houses. The presence of nitrite-reducing bacteria needs to be identified to prove the presence of these bacteria in swiftlet houses that have the potential to contribute to nitrite contamination in edible bird’s nest. This study aims to isolate nitrate-reducing bacteria in an effort to control nitrite using bacteriophages in the future. This study targeted nitrate-reducing bacteria collected from environmental samples (waste, feces, pond water, artificial pond water, soil, swiftlet eggshells, white edible bird’s nest (Aerodramus fuciphagus), black bird’s nest (Aerodramus maximus)) (n=40) from two different swiftlet houses in Sumedang, West Java, Indonesia. All isolates collected were subjected to a series of microbiological tests, phenotypic characterization (Gram staining, morphology, sugar fermentation ability, enzymes, etc.) and genotyping by PCR amplification and 16S rDNA gene sequencing. Raw sequencing data were analyzed using DNASTAR® software for DNA sequence alignment and phylogenetic tree construction. In the present work, four bacteria species were identified, including Priestia megaterium, Pseudomonas putida, Stenotrophomonas maltophilia, and Proteus terrae. To our knowledge, this study is the first report of nitrate-reducing bacteria isolated from birdhouses.
Addition of dextrose and caffeine to the intravenous treatment of milk fever in Holstein Friesian cattle Ady Kurnianto; Novianto Meska Pratama
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 3 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i3.2023.172-180

Abstract

A farmer reported a cow unable to stand 48 hours postpartum. The cow was a 2.5 years old dairy cow with a body weight of around 350 kg. The cow was fed elephant grass, standard concentrate (16–18%) crude protein, and drinking water ad libitum without mineral supplementation during the dry season. The cow's position and posture when lying down, ear temperature, and eye pupils were included in the physical examination. Availability of food and drink was examined to predict appetite and thirst. The results of the history and physical examination showed that the cow was unable to stand 48 hours after calving, had no appetite and eye reflexes, had cold ears, and trembling hind legs. The cow was laid on its right side, with its head turned to flank. Based on the anamnesis and physical examination, the cow was diagnosed as suffering from stage 2 puerperal hypocalcemia. The cow was infused via the jugular vein with a solution containing 270 mg calcium borogluconate, 70 mg magnesium borogluconate, 300 mg dextrose, 5 mg phosphorus element, 2 mg potassium chloride, and 5 mg caffeine per 1 mL of solution. Forty-five minutes after the infusion, the cow was able to stand, although it still looked weak. It could be concluded that the addition of dextrose and caffeine to the standard treatment of milk fever was beneficial for recovery.
Co-Authors Adilla Luthfita Fa’za Bagus Uda Palgunadi, Bagus Uda Evi Triana Novianto Meska Pratama Novik Nurhidayat Olan Rahayu Puji Astuti Nussa Roeswandono Roeswandono Siti Gusti Ningrum, Siti Gusti Titin Yulinery