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All Journal HAYATI Journal of Biosciences ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner SPEKTA (Jurnal Pengabdian Kepada Masyarakat : Teknologi dan Aplikasi) Makara Journal of Science Prosiding Seminar Nasional Kusuma
Siti Gusti Ningrum, Siti Gusti
Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Energy Environmental Science Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Veterinary

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Evaluation of Nitrite Concentration in Edible Bird’s Nest (White, Yellow, Orange, and Red Blood) Ningrum, Siti Gusti; Palgunad, Bagus Uda; Sasmita, Rochiman
Makara Journal of Science Vol. 26, No. 1
Publisher : UI Scholars Hub

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Abstract

The color of edible bird’s nest is associated with its nitrite concentration, but this relationship remains inconclusive. This investigation aimed to evaluate the nitrite content in edible bird’s nest of four different colors: white, yellow, orange, and red blood. Fifty-eight edible bird’s nest samples were obtained from five swiftlet farmhouses in Borneo Island, Indonesia and analyzed for nitrite content using Genesys 30 visible spectrophotometer. Results showed that the dark-colored edible bird’s nests (yellow, orange, and red blood) had higher nitrite concentrations of 304, 317, and 309 ppm, respectively, compared with the white-colored one (15 ppm). Therefore, the color of edible bird’s nest was associated with its nitrite concentration. This study provided updated information about the nitrite concentration in edible bird’s nest of various colors.
The Potency of Citrus aurantiifolia Swingle and Sea Salt Solution as a Cleansing Agent for Edible Bird’s Nests Ningrum, Siti Gusti; Candra, Adhitya Yoppy Ro; Wardhani, Hana Cipka Pramuda
Makara Journal of Science Vol. 27, No. 1
Publisher : UI Scholars Hub

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Abstract

The present study aimed to evaluate the potency of Citrus aurantiifolia Swingle and sea salt solution as a promising cleansing agent for edible bird’s nests (EBNs). Ascorbic acid, sodium, and chloride analyses of the C. aurantiifolia Swingle and sea salt solution were conducted using HPLC, ICP-OES, and titrimetry, respectively. The characteristics of physical samples and hydrogen peroxide detection were examined in this study. The reduction activity of the Citrus aurantiifolia Swingle and sea salt solution on the nitrite of 120 white EBNs was studied. This nitrite reduction activity was compared with the available method (standard), drinking water method, 6% C. aurantiifolia Swingle extraction method, and sea salt water. An organoleptic test was performed by three trained panelists to determine the color of the samples before and after treatments. The results showed that the C. aurantiifolia Swingle and sea salt solution contained 0.27 mg/100 mL of ascorbic acid, 76370.93 mg/L of sodium, and 7555.61 mg/100 mL of chloride. In comparison results, the C. aurantiifolia Swingle and sea salt solution can reduce nitrite levels up to 86%, remove hydrogen peroxide, and improve the natural color of EBNs. The present study provides the first potential future cleansing agent for EBNs.
Chicken Immune Responses to Vaccination by the Avian Influenza Subtype H5N1 against Avian Influenza Ramlah, Ramlah; Murtini, Sri; Soejoedono, Retno Damayanti; Natih, Ketut Karuni Nyanakumari; Ningrum, Siti Gusti
Makara Journal of Science Vol. 27, No. 3
Publisher : UI Scholars Hub

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Abstract

Avian Influenza (AI) is one of the strategic animal diseases still considered a priority for control by the Indonesian government. This study aimed to examine the immune response of chickens to various types of H5N1 subtype AI vaccines in Indonesia and to determine the correlation of factors influencing the post-vaccination AI antibody response. Serum samples were tested using the hemagglutination inhibition (HI) test with the standard AI antigen subtype H5N1 strain A/Chicken/Barru/BBVM/41-13/2013 (Clade 2.1.3) and strain A/Chicken/Semarang/04141225-07/2014 (Clade 2.3.2). Antibody titer was calculated using the Geometric Mean Titer (GMT). The correlation analyses were performed to assess the correlation of antibody titer against each of the following factors: age of chicken at the time of sampling, the interval between sampling time and the previous vaccination, and the number of vaccinations performed. The results showed that the average antibody titer value against the AI antigen subtype H5N1 strain A/Chicken/Barru/BBVM/41-13/2013 (Clade 2.1.3) was highest in samples from the South Sumatra Province, namely, Palembang City, which was 26.42 HIU. The results showed a significant correlation (p-value <0,05) between antibody titer and the interval between sampling time and the previous vaccination, the number of vaccination performed and age of chicken at the time of sampling. Therefore, results showed that the immunity developed from vaccination using the AI vaccine seed subtype H5N1 could induce immunity with a protective value of ≥16.
Application of SEAMON as Edible Bird’s Nest cleanser in the Edible Bird’s Nest Industry Ningrum, Siti Gusti; Yulianto , Andreas Berny; Purnamasari, Kartika
SPEKTA (Jurnal Pengabdian Kepada Masyarakat : Teknologi dan Aplikasi) Vol. 4 No. 2 (2023)
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/spekta.v4i2.8595

Abstract

Background: In Surabaya, the edible bird's nest industry has witnessed significant growth in recent years, driven by increasing demand for this highly prized delicacy, known for its nutritional and medicinal benefits. As the industry expands, effective cleaning methods for edible bird's nests become paramount. Contaminants, impurities, and feathers can compromise the quality and purity of edible bird's nests, affecting their market value and consumer appeal. Contribution: In response to this challenge, applying SEAMON as an edible bird's nest cleanser has emerged as a promising solution. SEAMON, a specific cleaning agent for edible birds' nests, offers the potential to revolutionize the cleaning process within the edible birds' nest industry. This innovative approach addresses the cleanliness and hygiene standards required for edible bird's nests, ensuring that consumers receive the highest quality product. Method: The SEAMON application was carried out at the edible bird's nest industry in Surabaya, East Java, on July 2, 2023, with twelve samples of edible bird's nests with heavy feather characteristics. All samples were tested for nitrite analysis using a Genesys 30 visible spectrophotometer. Results: Based on the results of its application, this innovation has the potential as an edible bird’s nest cleaning agent to clean hydrogen peroxide residue and can reduce nitrite levels in the white edible bird’s nest. Conclusion: This washing liquid is the first cleanser specifically for white edible bird’s nests, and another advantage is that it is food grade, so edible bird’s nests cleaned with SEAMON is safe for human consumption.
Isolation and Identification of Vibrio parahaemolyticus Bacteria in Bottlenose Dolphins (Tursiop truncates) in Kendal Conservation Pond, Central Java Nafiqoh, Nunak; Setiadi, Setiadi; Novita, Hessy; Lusiastuti, Angela Mariana; Indrawati, Agustin; Elmanaviean; Jannah, Siti Nur; Gardenia, Lila; Penataseputro, Tanjung; Andriyono, Sapto; Ningrum, Siti Gusti; Oktaviani, Dian; Syahidah, Dewi; Wattiheluw, Muhammad Subhan; Purwaningsih, Uni
HAYATI Journal of Biosciences Vol. 31 No. 5 (2024): September 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.5.811-819

Abstract

Aquatic mammals in Indonesia are officially protected. However, there is a lack of research on these mammals, particularly in relation to potential disease-causing bacteria. A study was conducted in a conservation pond to address this gap, where swab samples were taken from the blowholes of aquatic mammals. The focus was on identifying bacteria that could potentially cause infectious diseases in these animals. The results revealed V. parahaemolyticus bacterial isolates, which showed a 98% similarity to the registered V. parahaemolyticus in NCBI. These bacterial isolates exhibited hemolysin properties and demonstrated resistance to trimethoprim, streptomycin, cephalothin, and penicillin antibiotics.
Isolation of Aspergillus from edible-nest swiftlet feces in bird houses of Central Java Ningrum, Siti Gusti; Ginting, Eykel Arief Fortuna; Rahmawati, Indra
Jurnal Sain Veteriner Vol 42, No 3 (2024): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.95507

Abstract

Five edible-nest swiftlet houses in Central Java were sampled for fecal specimens. The samples were obtained using a sterile container to maintain aseptic conditions. Subsequently, the samples were mixed thoroughly in a tube containing sterile saline and allowed to incubate for a duration of 30 minutes. One milliliter of supernatant was placed onto Potato Dextrose Agar (PDA) with chloramphenicol (50 mg/L) and incubated at a temperature of 25 ºC for a duration of one week. The results showed that all samples had a high percentage of Aspergillus niger (100%), Aspergillus flavus (100%), and Aspergillus fumigatus (100%) isolates. The findings suggest that edible-nest swiftlet houses in Central Java can serve as a potential reservoir and conduit for fungal infection and transmission.
Isolasi Bakteriofag Dari Limbah Burung Walet Menggunakan Metode Plaque Assay Savitri, Ananda Maudya; Ningrum, Siti Gusti
Prosiding Seminar Nasional Kusuma Vol 2 (2024): Prosiding Seminar Nasional Kusuma
Publisher : LPPM UWKS

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Abstract

Latar belakang: Bakteriofag atau phage adalah virus yang menyerang sel bakteri. Karena fag adalah musuh alami bakteri yang sering terdapat dalam air limbah, fag dapat digunakan sebagai agen biokontrol yang efisien untuk mikroorganisme bawaan yang berbahaya. Manfaat bakteriofag adalah kemampuannya menghasilkan enzim lisozim, yang memungkinkan mereka menginfeksi dan melisiskan bakteri tertentu. Ini adalah salah satu manfaat penggunaan bakteriofag untuk melakukan biokontrol bakteri dengan cara yang lebih aman. Tujuan: Penelitian ini dilakukan untuk mengisolasi bakteriofag dari limbah di lingkungan rumah burung walet menggunakan metode plaque assay. Metode: Jenis penelitian ini adalah penelitian deskriptif observational laboratorik. Pengambilan sampel dalam penelitian ini dilakukan secara acak dengan menggunakan metode purposive sampling. Sampel diambil dari 2 rumah burung walet, dengan jumlah 20 sampel limbah burung walet yang diperoleh dari rumah burung walet di Sumedang. Kultur bakteri yang digunakan sebagai inang untuk isolasi bakteriofag adalah Lysinibacillus sp. Spot test dilakukan dengan mencampurkan 500μl bakteri dan TSA semisolid lalu dituangkan ke media TSA kemudian filtrat bakteriofag di teteskan sebanyak 10μl dan diinkubasi. Setelah zona bening muncul maka dilakukan plaque assay dengan pengenceran 10-1 – 10-6 kemudian masing-masing pengenceran dicampur ke TSA dan diinkubasi pada suhu 30°C. Picking plaque dilakukan dengan mengambil single plaque. Hasil: Hasil spot test dalam penelitian ini terdapat 2 sampel LHW dan LPW yang menunjukkan zona clear, hasil plaque assay diperoleh satu sampel LPW dengan titer 2,5 x 105 PFU/ml. Berdasarkan hasil penelitian yang telah dilakukan, isolasi bakteriofag dari limbah burung walet menggunakan metode plaque assay dapat mengisolasi satu bakteriofag terhadap bakteri penghasil nitrit Lysinibacillus sp. Kesimpulan: Penelitian ini berhasil mengisolasi bakteriofag terhadap Lysinibacillus sp.
Isolation and Molecular Identification of Nitrate-Reducing Bacteria from Swiftlet Houses in Sumedang, West Java Ningrum, Siti Gusti; Novik Nurhidayat; Titin Yulinery; Evi Triana; Olan Rahayu Puji Astuti Nussa; Ady Kurnianto
Acta VETERINARIA Indonesiana Vol. 13 No. 1 (2025): Maret 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.1.1-7

Abstract

Edible bird‘s nest is an important export commodity that is currently of concern to the Indonesian government. One of the requirements for exporting edible bird’s nest to the People's Republic of China is that this product must meet the requirements for nitrite content in the product below 30 ppm. This nitrite contamination can be obtained from the results of nitrogen metabolism from nitrifying bacteria. However, information on nitrite-reducing bacteria in swiftlet houses has never been reported and is urgent in controlling nitrifying bacteria in swiftlet houses. The presence of nitrite-reducing bacteria needs to be identified to prove the presence of these bacteria in swiftlet houses that have the potential to contribute to nitrite contamination in edible bird’s nest. This study aims to isolate nitrate-reducing bacteria in an effort to control nitrite using bacteriophages in the future. This study targeted nitrate-reducing bacteria collected from environmental samples (waste, feces, pond water, artificial pond water, soil, swiftlet eggshells, white edible bird’s nest (Aerodramus fuciphagus), black bird’s nest (Aerodramus maximus)) (n=40) from two different swiftlet houses in Sumedang, West Java, Indonesia. All isolates collected were subjected to a series of microbiological tests, phenotypic characterization (Gram staining, morphology, sugar fermentation ability, enzymes, etc.) and genotyping by PCR amplification and 16S rDNA gene sequencing. Raw sequencing data were analyzed using DNASTAR® software for DNA sequence alignment and phylogenetic tree construction. In the present work, four bacteria species were identified, including Priestia megaterium, Pseudomonas putida, Stenotrophomonas maltophilia, and Proteus terrae. To our knowledge, this study is the first report of nitrate-reducing bacteria isolated from birdhouses.
Co-Authors ady kurnianto Agustin Indrawati ANGELA MARIANA LUSIASTUTI Candra, Adhitya Yoppy Ro Elmanaviean Evi Triana Ginting, Eykel Arief Fortuna Hana Cipka Pramuda Wardhani INDRA RAHMAWATI Lila Gardenia Nafiqoh, Nunak Natih, Ketut Karuni Nyanakumari Novik Nurhidayat Novita, Hessy Oktaviani, Dian Olan Rahayu Puji Astuti Nussa Palgunad, Bagus Uda Penataseputro, Tanjung Purnamasari, Kartika Ramlah Ramlah Retno Damayanti Soejoedono Rochiman Sasmita Sapto Andriyono Savitri, Ananda Maudya Setiadi Setiadi Siti Nur Jannah sri murtini . Syahidah, Dewi Titin Yulinery Uni Purwaningsih Wattiheluw, Muhammad Subhan Yulianto , Andreas Berny