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All Journal Proceeding Biology Education Conference Journal of Biodiversity and Biotechnology
Parjanto Parjanto
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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ANALISIS POLA PITA-C KROMOSOM TANAMAN SALAK JANTAN DAN BETINA (Salacca zalacca var. zalacca) Parjanto Parjanto
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 7, No 1 (2010): Seminar Nasional VII Pendidikan Biologi
Publisher : Universitas Sebelas Maret

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ABSTRACT The objectives of this research were to study the C-banding pattern of male and female salak chromosome and to obtain the sex marker of salak based on C-banding pattern variation. C-banding pattern of male and  female of salak were observed using geimsa C-banding technique. Variation of chromosomes C-banding pattern were analyzed according to number and position of C-band of chromosomes. The results of the  research showed that C-band of salak chromosom can be revealed using geimsa C-banding technique. The C-band revealed on the telomer and  the centromer region.  Pro-metaphase and metaphase chromosomes could be used to observe the C-band.  There were no differences in C-band pattern of chromosome of male and female salak, so the sex of  salak  can not be identified based on the observation of C-band of chromosome. Key words: chromosome, C-banding, sex marker, salak ABSTRAK   Tujuan penelitian ini adalah mempelajari pola pita-C kromosom  salak jantan dan betina, selanjutnya mengidentifikasi penanda kelamin tanaman salak berdasar perbedaan pola pita-C kromosom.Tanaman salak jantan dan betina varietas pondoh asal cangkok anakan digunakan untuk mempelajari perbedaan pola pita-C. Pengamatan pita-C kromosom dilakukan dengan teknik pemitaan geimsa (geimsa C-banding technique).  Analisis perbedaan pola pita-C kromosom salak jantan dan  betina dilakukan berdasar jumlah dan posisi pita-C dengan membandingkan karyogram dan idiogram pita-C. Hasil penelitian menunjukkan bahwa pita-C kromosom salak dapat dihasilkan menggunakan  metode pemitaan-C geimsa (Geimsa C-banding technique), sebagian pita-C berada pada ujung kromosom atau terletak pada daerah telomer dan sebagian yang lain terletak di daerah sentromer. Pita-C dapat dihasilkan pada kromosom metafase awal (pro metaphase) maupun kromosom metafase (late metaphase).  Tidak terdapat perbedaan pola pita-C  antara kromosom tanaman salak jantan dan betina, sehingga jenis kelamin tanaman salak tidak didapatkan ditentukan berdasar pola pita-C kromosom.   Kata kunci: kromosom,  pita-C, penanda kelamin, salak.
Yield Performance of Short-Stem Promising Mutant Lines of Mentik Wangi Rice Induced by 200 Gy Gamma Irradiation Fitria Roviqowati; Desfinenda Calistarajni; Parjanto Parjanto; Edi Purwanto; Ahmad Yunus
Journal of Biodiversity and Biotechnology Vol 4, No 2 (2024)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v4i1.106011

Abstract

The demand for rice in Indonesia continues to increase but production has decreased. Efforts that can be made are the use of local varieties of mentik wangi rice. Mentik wangi rice has the disadvantages of long life, high stems, and low crop production. As a solution, plant breeding is carried out in the form of mutation by giving gamma irradiation which can improve unwanted genetic traits and produce superior varieties with the dose used of 200 gray. This study aims to evaluate the yield potential of promising mutant strains with 200 gray gamma irradiation on mentik wangi rice and get mutant strains that have superior criteria for short-stemmed, short-lived, and high-yielding. This research was conducted in the rice field of Ngampel Hamlet, Gentungan Village, Mojogedang, Karanganyar, Central Java. The research was conducted in June-October 2023. The design used was a Randomized Complete Group Design (RCBD) with a single factor, namely strains consisting of 14 mutant strains and 1 control strain with 3 repetitions. The results of observations were analyzed by Analysis of Variance (ANOVA) at the 5% level and continued with Duncan Multiple Range Test (DMRT) at the 5% level. All tested strains produced the expected superior criteria, namely short stem, short age, and high productivity.
Co-Authors Ahmad Yunus Desfinenda Calistarajni Edi Purwanto Fitria Roviqowati