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Seleksi, Karakterisasi dan Identifikasi Bakteri Pendegradasi 2-(thiocyanomethylthio) benzothiazole (TCMTB) Sembiring, Langkah; Susilawati, Lela; Suhartanti, Dwi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

The objective of this research was to investigate the capabilities of bacteria isolated from industrial tanning waste to degrade TCMTB. The bacteria was initialy screened, based on their tolerance to various concentration of TCMTB using paper disk method. Then, those strains were further analyzed in terms of their ability to produce ammonia (NH4+) and sulphate (SO42-). Degradation activity was measured based on remaining residue of TCMTB analyzed using HPLC. The superior strain that showed the highest activity in degradation of TCMTB then were characterized and identified based on phenotypic and 16S rDNA sequence analysis. The result of the experiments showed that four selected strains among seven were choosen based on their high tolerance to various concentration of TCMTB, namely PK1, PK2, PK4 and PK6. All four strains showed the ability to produce ammonia and sulphate but three of which, namely PK2, PK4 and PK6 showed the high capability to degrade TCMTB. One particular strain (PK2) was observed to degrade TCMTB 40.8% within 7 days, but the others were less than 30%. Based on the phenotypic characteristics and 16S rDNA sequence analysis, the best strains (PK2) was identified to be member of genus Pseudomonas.

Viabilitas Sel Bakteri Dengan Cryoprotectant Agents Berbeda (Sebagai Acuan Dalam Preservasi Culture Collection di Laboratorium Mikrobiologi) Lela Susilawati; Ethik Susiawati Purnomo
Biogenesis: Jurnal Ilmiah Biologi Vol 4 No 1 (2016)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v4i1.1118

The aim of this research was to determine the viability of bacterial cells on different cryoprotectant agents. Three kind of cryoprotectant agents and two types of mixed cryprotectant were applied to evaluate the ability of bacteria to growth on Agar medium. Four isolates were used namely Escherichia coli ATCC 35218, Staphylococcus aureus ATCC 25923, Shigella flexneri ATCC 12022 and Bacillus subtilis ATCC 6051. All strains were preserved in 10% skimmed milk, 10% glycerol, 10% DMSO, 10% glycerol:10% skimmed milk and 10% DMSO:10% skimmed milk at -80°C for sixty days. Immediately after preserved, the bacteria were tested their viability at day 30 and 60 during storage. The enumeration of samples taken from cultures in NA media. Better survival of bacterial cells after freezing was shown in all tested cryprotectant agents. Highest microbial viability was shown in 10% skimmed milk, 10% glycerol and mixed 10%DMSO:10% skimmed milk. Hence, those three types of cryoprotectant agents can be recommended to preserve bacterial cell for long term storage.

Seleksi, Karakterisasi dan Identifikasi Bakteri Pendegradasi 2-(thiocyanomethylthio) benzothiazole (TCMTB) Langkah Sembiring; Lela Susilawati; Dwi Suhartanti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v13i3.2565

The objective of this research was to investigate the capabilities of bacteria isolated from industrial tanning waste to degrade TCMTB. The bacteria was initialy screened, based on their tolerance to various concentration of TCMTB using paper disk method. Then, those strains were further analyzed in terms of their ability to produce ammonia (NH4+) and sulphate (SO42-). Degradation activity was measured based on remaining residue of TCMTB analyzed using HPLC. The superior strain that showed the highest activity in degradation of TCMTB then were characterized and identified based on phenotypic and 16S rDNA sequence analysis. The result of the experiments showed that four selected strains among seven were choosen based on their high tolerance to various concentration of TCMTB, namely PK1, PK2, PK4 and PK6. All four strains showed the ability to produce ammonia and sulphate but three of which, namely PK2, PK4 and PK6 showed the high capability to degrade TCMTB. One particular strain (PK2) was observed to degrade TCMTB 40.8% within 7 days, but the others were less than 30%. Based on the phenotypic characteristics and 16S rDNA sequence analysis, the best strains (PK2) was identified to be member of genus Pseudomonas.

KERAGAMAN KARAKTER MORFOLOGI BAKTERI INDIGENOUS YANG DIISOLASI DARI LENDIR KATAK SAWAH (F. CANCRIVORA) LOKAL PADA BAGIAN DORSAL DAN VENTRAL Lela Susilawati; Afrizka Premana Sari
Kaunia: Integration and Interconnection Islam and Science Vol. 9 No. 2 (2013)
Publisher : Fakultas Sains dan Teknologi UIN Sunan Kalijaga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/kaunia.1056

F. cancrivora is an abundance species in Indonesia. It is believed that indigenous bacteria isolated from F. cancrivora have many bioactive substances. Different habitat of F. cancrivora will influence the bacteria lived in their mucous. Hence, comparing the morphological properties among them is one of preliminary assay to explore their potential as bioactive substances’ producer. The present work explored the diversity of morphological characteristic of indigenous bacteria isolated from skin mucous of F. cancrivora. The bacteria were isolated from dorsal and ventral skin mucous using swab method. The colonies that appeared on nutrient agar were then purified and characterized based on the colony and cell properties. A total 20 isolates were obtained, 11 of them are from dorsal site and 9 isolates are from ventral site. The morphological characteristic of those isolates showed that the bacteria isolated from dorsal site have higher diversity in term of colonies and cell characteristic than bacteria isolated from ventral site of frog skin mucous.

Potensi Fusarium sp. dari Tanaman Pisang sebagai Inokulan Pembentuk Gubal pada Tanaman Gaharu Wilisiani, Fariha; Susilawati, Lela; Sumardi, Sumardi; Yuslinawari, Yuslinawari; Widyanto, Dwi; Kristiyani, Cicilia Dewi; Khasanah, Uswatun; Rofiah, Ngalimatur
Jurnal Agroekoteknologi Tropika Lembab Vol 6, No 2 (2024): Agroekoteknologi Tropika Lembab Volume 6 Nomor 2 Februari 2024
Publisher : Mulawarman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35941/jatl.6.2.2024.14059.18-22

Indonesia merupakan salah satu produsen gaharu terbesar di dunia dengan jumlah ekspor yang terus meningkat. Gaharu yang banyak diperdagangkan selama ini adalah gaharu yang terbentuk secara alami, sehingga berdampak pada banyaknya eksploitasi hutan alam yang tidak terkendali. Dalam budidaya tanaman penghasil gaharu, salah satu kendala yang ditemui yaitu pembentukan gaharu dengan rendemen rendah serta proses pembentukan gubal lama. Saat ini banyak dikembangkan pembentukan gaharu secara buatan dengan inokulasi Fusarium sp. untuk mempercepat pembentukan gubal. Isolat Fusarium sp. dari beberapa tanaman inangnya memiliki potensi sebagai inokulan pembentuk gubal gaharu. Dalam penelitian ini dikaji tentang inokulan Fusarium BD2 dari tanaman pisang bergejala layu Fusarium dalam pembentukan gubal gaharu. Inokulasi dilakukan dengan metode infus (kepadatan spora 106 spora mL-1) sebanyak 50 mL pada pohon gaharu yang telah dibor). Hasil inokulasi teramati adanya perubahan warna kayu gaharu di sekitar titik inokulasi. Kesimpulan penelitian ini yaitu inokulan Fusarium isolat BD2 dari tanaman pisang memiliki potensi sebagai inokulan gaharu.

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