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All Journal Jurnal Penelitian Kelapa Sawit
Enrique Ritter
Neiker Tecnalia
Agriculture, Biological Sciences & Forestry

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KONSTRUKSI PETA GENETIK KELAPA SAWIT (Elaeis guineensis JACQ.) PPKS KETURUNAN SP540T DENGAN MENGGUNAKAN MARKA RESTRICTION SITE ASSOCIATED DNA (RAD) Retno Diah Setiowati; Enrique Ritter; Sri Wening; Yurna Yenni; Edy Suprianto
Jurnal Penelitian Kelapa Sawit Vol 25 No 1 (2017): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (486.309 KB) | DOI: 10.22302/iopri.jur.jpks.v25i1.21

Abstract

Construction of genetic linkage on oil palm derived SP540T was established in 2012-2015 in colaboration with PPKS, Indonesia with Neiker, Spain. DNA isolation was conducted in Molecular Biology Laboratory in PPKS Marihat meanwhile RAD analysis was performed in Laboratorio Biotecnologia Vegetal, Neiker, Spain. Genetic map construction was carried out using Restriction Site Associated DNA. A total 532 RAD fragments were applied to configure 16 Linkage Groups (LGs) which represented 16 chromosomes of oil palm. The map spans in 1269.9 map unit (mU) or 79.37 mU per chromosome in average and consists of RAD fragments which could be blasted to the DNA data base to identify candidate genes. Genenic map which had been formed is a fondation of constructing an IOPRI functional map (IOPRImap) when it is integrated with phenotypic data. The IOPRImap is expected to facilitate shortening oil palm selection cycle and defining a precise oil palm breeding strategy.
Co-Authors Edy Suprianto Retno Diah Setiowati Sri Wening Yurna Yenni