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Yurna Yenni

Indonesian Oil Palm Research Institute

Author-ID : 3464682

Agriculture, Biological Sciences & Forestry

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KETELUSURAN GENETIK KETURUNAN KELAPA SAWIT (Elaeis guineensis Jacq.) MENGGUNAKAN SIMPLE SEQUENCE REPEAT (SSR) Rokhana Faizah; Sri Wening; Yurna Yenni; Sujadi Sujadi
Jurnal Penelitian Kelapa Sawit Vol 24 No 3 (2016): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Progenies traceability status becomes an important activity in the oil palm breeding program, especially to obtain propriety of individual and progenies population with their parents. This study is concerned to analyze genetic traceability based on pattern and allele size of their parent using Simple Sequence Repeat (SSR) markers. There were 90 palms from 7 progenies populations in the AD02S, AD03S Adolina and MA22S Marihat Estate PT. Perkebunan Nusantara IV used in this research. Leaf and pollen samples were used to get genomic DNA. Then, DNA genomic was amplified using multiplexing method of 8 SSR markers and fluorescence labels of 6-FAM, HEX, and NED. Fragment analysis and extracted genotype data was obtained using Gene Marker® versi 2.4.0 Soft Genetics® LLC program. Genetic traceability analysis was based on alelle segregation pattern of Mendelian Law. The results described inappropriate alleles 4 individuals from 3 progenies (N, P, and S). Those individuals were palmrow of 22-30 in the AD02S; 29-27 in the MA22S; 9-22 and 9-28 in the AD02S. Other 4 progenies showed an appropriate segregation of genotype with their parents, which are crosses number of M, Q, R, and O.

KONSTRUKSI PETA GENETIK KELAPA SAWIT (Elaeis guineensis JACQ.) PPKS KETURUNAN SP540T DENGAN MENGGUNAKAN MARKA RESTRICTION SITE ASSOCIATED DNA (RAD) Retno Diah Setiowati; Enrique Ritter; Sri Wening; Yurna Yenni; Edy Suprianto
Jurnal Penelitian Kelapa Sawit Vol 25 No 1 (2017): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Construction of genetic linkage on oil palm derived SP540T was established in 2012-2015 in colaboration with PPKS, Indonesia with Neiker, Spain. DNA isolation was conducted in Molecular Biology Laboratory in PPKS Marihat meanwhile RAD analysis was performed in Laboratorio Biotecnologia Vegetal, Neiker, Spain. Genetic map construction was carried out using Restriction Site Associated DNA. A total 532 RAD fragments were applied to configure 16 Linkage Groups (LGs) which represented 16 chromosomes of oil palm. The map spans in 1269.9 map unit (mU) or 79.37 mU per chromosome in average and consists of RAD fragments which could be blasted to the DNA data base to identify candidate genes. Genenic map which had been formed is a fondation of constructing an IOPRI functional map (IOPRImap) when it is integrated with phenotypic data. The IOPRImap is expected to facilitate shortening oil palm selection cycle and defining a precise oil palm breeding strategy.

JARAK GENETIK 47 AKSESI PLASMA NUTFAH KELAPA SAWIT (Elaeis guineensis Jacq.) ASAL KAMERUN BERDASARKAN KARAKTER MORFOLOGI Sujadi Sujadi; Tiara S Wandita; Nanang Supena; Yurna Yenni
Jurnal Penelitian Kelapa Sawit Vol 27 No 1 (2019): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Oil palm (Elaeis guineensis Jacq.) is a plantation commodity that has an important role in various aspects of life in Indonesia, especially the domestic economy. This is supported by the increasing demand for world palm oil for food needs (edible oil), industrial (oleochemical), and alternative energy sources based on biodiesel. The development of the palm oil industry requires several efforts to achieve increased national productivity, one of which is the utilization of quality seeds supported by the availability of genetic resources (germplasm) that have a high level of genetic diversity. Efforts that can be made for the development of oil palm in Indonesia is through the characterization of oil palm intoduction from abroad. Considering this, further research on genetic distance analysis of palm oil accession introduced from Cameroon is based on morphological characters to produce parent elders that can produce heterosis properties. Research conducted at Seed Garden of Adolina PTPN IV and laboratory analysis of plant material of Palm Oil Research Center located on Jl. Brigjen Katamso No. 5, Medan, North Sumatera, which lasted from December 2017 to February 2018. Single observations were made on 47 accession palm oil from Cameroon planted on December 2010 and ten trees from PPKS 540 variety for compare. Data analysis used was a description of the plant to know the character of plant morphology as well as genetic distance analysis. Analysis of genetic distance using PCA analysis and cluster analysis. The results obtained are based on the results of PCA (Principal Component Analysis) reduce the observed character into six major components that have eigen value > 1 and able to explain the material diversity tested for 73.8%. Based on the cluster analysis obtained the genetic distance of 47 accessions of palm oil from Cameroon by 57%. It can be concluded that, when the genetic distance between accessions is further away, the larger the genetic variability between the observed characters. If the genetic diversity is wider the greater the chance for successful selection in increasing the desired gene frequency.

EVALUASI KARAKTER KOMPAK HASIL PENGUJIAN KETURUNAN SIKLUS KETIGA PROGRAM PEMULIAAN KELAPA SAWIT PUSAT PENELITIAN KELAPA SAWIT Ikhwan Fadli Pangaribuan; Yurna Yenni; Sujadi Sujadi; Edy Suprianto
Jurnal Penelitian Kelapa Sawit Vol 27 No 3 (2019): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

One of the secondary characters desired by oil palm planters is compact palm. Compact characters are characterized by slow height increment rate and short rachis length. Both characters are able to extend the economic life of oil palm and increase palm productivity. This paper will discuss the results of the evaluation of compact characters in several crosses of the third cycle progeny test of RRS (Reciprocal Recurrent Selection) program. The field trial was carried out on 21 crosses planted in 2008 at Dolok Sinumbah Estate. The observations showed that cross no. BJ62/05 and BJ05/05 have the potential to produce compact palm with the slowest height increment rates, the shortest rachis, and low LAI values. With these characters, these crosses can be planted at higher planting densities. Parents no. BJ5633D and 93-133-4T also have the potential to produce offspring with compact characters.

Studi Asosiasi Genom (GWAS) Komponen Tandan Populasi Interspesifik Elaeis oleifera dan Elaeis guineensis Heri Adriwan Siregar; Edy Suprianto; Sujadi Sujadi; Hernawan Y Rahmadi; Mohamad Arif; Retno Diah Setiowati; Nanang Supena; Rokhana Faizah; Sri Wening; Yurna Yenni; Abdul Razak Purba
Jurnal Penelitian Kelapa Sawit Vol 29 No 2 (2021): Jurnal Peneltian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iopri.jur.jpks.v29i2.144

The oil palm breeding program for the species Elaeis guineensis and the backcross Elaeis oleifera is running slowly because oil palm is an annual plant. Therefore, it is necessary to have an alternative approach that can accelerate the oil palm breeding program. The SNP (single nucleotide polymorphism) genome-wide approach was then used to study the association between 18 phenotypes of bunch component in oil palm germplasm of E. oleifera from Suriname and Brazil Coari, some interspecific hybrids and some elite progeny of E. guineensis. The genotyping by sequencing (GBS) analysis produced a total of 459 million or approximately 798 thousand reads per sample and 3,252 SNPs were eligible for 456 genotypes. Using various association models, eleven normalized phenotypic data showed significant associations with 29 SNPs. Based on the annotations, 17 SNPs were related to genes wtih certain biological functions. Three SNPs were found to be at the exon of a gene, namely SNP4416, SNP349 and SNP3865, while the other 15 SNPs were at the intragenic to a gene. Four SNPs are common SNPs in phenotypes C16:0 and C18:1 as weel as in C20 0 and C20:1. This research shows the potential of SNPs that can be used as an alternative approach to E. oleifera backcross breeding, although further research is needed for validation purposes.

Studi pendahuluan kandungan Vitamin E pada varietas kelapa sawit PPKS Retno Diah Setiowati; Yurna Yenni; Frisda Rimbun Panjaitan; Sujadi Sujadi; Mahmud Irfan Lubis; Ernayunita Ernayunita
Jurnal Penelitian Kelapa Sawit Vol 29 No 3 (2021): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iopri.jur.jpks.v29i3.153

An oil palm variety with high vitamin E has an added value because of its benefit as pharmaceutical and nutraceutical source. The measurement of the vitamin E content in CPO from eight varieties of oil palm is an effort to obtain high vitamin E varieties with the optimum oil yield. The varieties used in this experiment were DxP PPKS 718, DxP PPKS 239, DxP PPKS 540, DxP Yangambi, DxP Lame, DxP Avros, DxP Simalungun, and DxP Langkat, which were planted in a demonstration block, located at Kebun Adolina PTPN IV. The result showed that the average of the vitamin E from 8 varieties ranged from 477.36 ppm up to 582.78 ppm. The DxP Yangambi has the potency to be improved as the candidate of DxP variety with high vitamin E added value due to its highest vitamin E content. On the other hand, the DxP PPKS 540 is appropriate as candidate of ortets for high vitamin E clones regarding the vitamin E content; which is the highest over the whole samples

Co-Authors Abdul Razak Purba Edy Suprianto Enrique Ritter Ernayunita Ernayunita Frisda Rimbun Panjaitan Heri Adriwan Siregar Hernawan Y Rahmadi Ikhwan Fadli Pangaribuan Mahmud Irfan Lubis Mohamad Arif Nanang Supena Retno Diah Setiowati Rokhana Faizah Sri Wening Sujadi Sujadi Sujadi Sujadi Tiara S Wandita

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