Retno Diah Setiowati
Indonesian Oil Palm Research Institute

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KERAGAAN VEGETATIF DAN KOMPONEN TANDAN DARI SILANG BALIK SEMU PERTAMA Elaeis oleifera ORIGIN BRAZIL DAN SURINAME DI SUMATRA UTARA, INDONESIA Heri Adriwan Siregar; Hernawan Yuli Rahmadi; Retno Diah Setiowati; Edy Suprianto
Jurnal Penelitian Kelapa Sawit Vol 26 No 1 (2018): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (213.104 KB) | DOI: 10.22302/iopri.jur.jpks.v26i1.13

Abstract

An Attempt to combine the superior traits of Elaeis oleifera and Elaeis guineensis have been done through an interspecific hybrid cross and followed by pseudo-backcross 1 (pBC1). Observation of vegetative morphology and bunch components are presented in this paper. Two populations of pBC1 E. oleifera from the Suriname and Brazil origin were planted in 1990, 1993, 1995, and 2005, and were intensively observed for vegetative morphological properties and bunch components in November 2016 to February 2018. The results showed that almost all the individuals of pBC1 grew upright such as E. guineensis, no longer growing horizontally like the wild E. oleifera and the interspecific hybrid populations. The datas showed that the Suriname population plant architecture are compact or smaller than the Brazilian origin including the height increment and the size of the stem, the frond architecture and its components. Similarly, the bunch components show that the pBC1 Brazil is slightly superior to Suriname pBC1.
KONSTRUKSI PETA GENETIK KELAPA SAWIT (Elaeis guineensis JACQ.) PPKS KETURUNAN SP540T DENGAN MENGGUNAKAN MARKA RESTRICTION SITE ASSOCIATED DNA (RAD) Retno Diah Setiowati; Enrique Ritter; Sri Wening; Yurna Yenni; Edy Suprianto
Jurnal Penelitian Kelapa Sawit Vol 25 No 1 (2017): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (486.309 KB) | DOI: 10.22302/iopri.jur.jpks.v25i1.21

Abstract

Construction of genetic linkage on oil palm derived SP540T was established in 2012-2015 in colaboration with PPKS, Indonesia with Neiker, Spain. DNA isolation was conducted in Molecular Biology Laboratory in PPKS Marihat meanwhile RAD analysis was performed in Laboratorio Biotecnologia Vegetal, Neiker, Spain. Genetic map construction was carried out using Restriction Site Associated DNA. A total 532 RAD fragments were applied to configure 16 Linkage Groups (LGs) which represented 16 chromosomes of oil palm. The map spans in 1269.9 map unit (mU) or 79.37 mU per chromosome in average and consists of RAD fragments which could be blasted to the DNA data base to identify candidate genes. Genenic map which had been formed is a fondation of constructing an IOPRI functional map (IOPRImap) when it is integrated with phenotypic data. The IOPRImap is expected to facilitate shortening oil palm selection cycle and defining a precise oil palm breeding strategy.
Studi Asosiasi Genom (GWAS) Komponen Tandan Populasi Interspesifik Elaeis oleifera dan Elaeis guineensis Heri Adriwan Siregar; Edy Suprianto; Sujadi Sujadi; Hernawan Y Rahmadi; Mohamad Arif; Retno Diah Setiowati; Nanang Supena; Rokhana Faizah; Sri Wening; Yurna Yenni; Abdul Razak Purba
Jurnal Penelitian Kelapa Sawit Vol 29 No 2 (2021): Jurnal Peneltian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iopri.jur.jpks.v29i2.144

Abstract

The oil palm breeding program for the species Elaeis guineensis and the backcross Elaeis oleifera is running slowly because oil palm is an annual plant. Therefore, it is necessary to have an alternative approach that can accelerate the oil palm breeding program. The SNP (single nucleotide polymorphism) genome-wide approach was then used to study the association between 18 phenotypes of bunch component in oil palm germplasm of E. oleifera from Suriname and Brazil Coari, some interspecific hybrids and some elite progeny of E. guineensis. The genotyping by sequencing (GBS) analysis produced a total of 459 million or approximately 798 thousand reads per sample and 3,252 SNPs were eligible for 456 genotypes. Using various association models, eleven normalized phenotypic data showed significant associations with 29 SNPs. Based on the annotations, 17 SNPs were related to genes wtih certain biological functions. Three SNPs were found to be at the exon of a gene, namely SNP4416, SNP349 and SNP3865, while the other 15 SNPs were at the intragenic to a gene. Four SNPs are common SNPs in phenotypes C16:0 and C18:1 as weel as in C20 0 and C20:1. This research shows the potential of SNPs that can be used as an alternative approach to E. oleifera backcross breeding, although further research is needed for validation purposes.
Studi pendahuluan kandungan Vitamin E pada varietas kelapa sawit PPKS Retno Diah Setiowati; Yurna Yenni; Frisda Rimbun Panjaitan; Sujadi Sujadi; Mahmud Irfan Lubis; Ernayunita Ernayunita
Jurnal Penelitian Kelapa Sawit Vol 29 No 3 (2021): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iopri.jur.jpks.v29i3.153

Abstract

An oil palm variety with high vitamin E has an added value because of its benefit as pharmaceutical and nutraceutical source. The measurement of the vitamin E content in CPO from eight varieties of oil palm is an effort to obtain high vitamin E varieties with the optimum oil yield. The varieties used in this experiment were DxP PPKS 718, DxP PPKS 239, DxP PPKS 540, DxP Yangambi, DxP Lame, DxP Avros, DxP Simalungun, and DxP Langkat, which were planted in a demonstration block, located at Kebun Adolina PTPN IV. The result showed that the average of the vitamin E from 8 varieties ranged from 477.36 ppm up to 582.78 ppm. The DxP Yangambi has the potency to be improved as the candidate of DxP variety with high vitamin E added value due to its highest vitamin E content. On the other hand, the DxP PPKS 540 is appropriate as candidate of ortets for high vitamin E clones regarding the vitamin E content; which is the highest over the whole samples