Agung Subrata
Department Of Animal Science, Faculty Of Animal And Agricultural Sciences, Diponegoro University, Semarang, 50275, Indonesia

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PENGARUH PROTEKSI PROTEIN AMPAS KECAP DENGAN TANIN TERHADAP KONSENTRASI AMONIA, PRODUKSI PROTEIN TOTAL DAN PERSENTASE RUMEN UNDEGRADED DIETARY PROTEIN SECARA IN VITRO Mayangsari, Nadia Sitha; Subrata, Agung; Christiyanto, Marry
Animal Agriculture Journal Vol 2, No 1 (2013): Volume 2, Nomor 1, Tahun 2013
Publisher : Animal Agriculture Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (129.495 KB)

Abstract

ABSTRAK Penelitian bertujuan untuk mengetahui pengaruh level penambahan tanin daun mangrove untuk memproteksi protein ampas kecap terhadap konsentrasi amonia (NH3), produksi protein total dan persentase rumen undegraded dietary protein (RUDP) secara in vitro. Materi yang digunakan adalah ampas kecap, daun mangrove sebagai sumber tanin alami, cairan rumen dan reagensia yang digunakan dalam analisis NH3, protein total dan RUDP. Parameter yang diamati adalah konsentrasi NH3, produksi protein total dan persentase RUDP. Penelitian dilaksanakan menggunakan Rancangan Acak Lengkap dengan 4 perlakuan dan 4 ulangan. Perlakuan adalah T0 (ampas kecap + tanpa tanin), T1 (ampas kecap + tanin 0,25%), T2 (ampas kecap + tanin 0,50%), T3 (ampas kecap + tanin 0,75%). Hasil penelitian diolah secara statistik dengan analisis sidik ragam dan jika terdapat pengaruh nyata akibat perlakuan dilanjutkan dengan uji Duncan dengan taraf 5% untuk mengetahui adanya perbedaan pengaruh antar perlakuan. Hasil penelitian didapatkan konsentrasi NH3 protein ampas kecap terproteksi dengan perbedaan aras tanin berturut-turut adalah 4,34; 3,46; 3,29; 3,10 mM. Produksi protein total yang dihasilkan berturut-turut adalah 315,37; 330,05; 334,04; 304,45 mg/g. Persentase RUDP yang dihasilkan berturut-turut adalah 27,91; 27,96; 28,03; 27,36 %. Hasil uji variansi menunjukkan bahwa proteksi protein ampas kecap menggunakan tanin mangrove berpengaruh terhadap konsentrasi NH3 namun tidak berpengaruh terhadap produksi protein total dan persentase RUDP. Hasil uji lanjut DMRT menunjukkan bahwa konsentrasi NH3 pada perlakuan T0 nyata lebih tinggi (p<0,05) dari T1, T2 dan T3 sedangkan T1, T2 dan T3 tidak berbeda nyata (p>0,05). Dari hasil penelitian dapat disimpulkan bahwa proteksi protein ampas kecap menggunakan tanin daun mangrove terbukti menurunkan konsentrasi amonia (NH3) tetapi tidak mempengaruhi produksi protein total dan persentase RUDPnya.Kata kunci: ampas kecap; tanin; amonia; protein totalABSTRACTThe aimed of this research was to determine the effect of level tannin of mangrove leaf to protected soy pulp protein on ammonia concentration (NH3), total protein production and percentage of rumen undegraded dietary protein (RUDP) in vitro. The material used is soy pulp, mangroves leaves as a natural source of tannins, rumen liquor and reagents used in the analysis of NH3, total protein and RUDP. Parameters measured were NH3 concentrations, total protein production and percentage of RUDP. The experiment was conducted used a completely randomized designed with 4 treatments and 4 replications. Treatments are T0 (soy pulp + without tannin), T1 (soy pulp + tannin 0.25%), T2 (soy pulp + tannin 0.50%), T3 (soy pulp + tannin 0.75%). The results were analyzed statistically by variance analysis, and if there is a real effect due to treatment followed by Duncan test at 5% level to detect a difference between treatment effects. The results obtained NH3 concentration of soy pulp protein protected with different tannin are 4.34; 3.46; 3.29; 3.10 mM. The results total protein production are 315.37; 330.05; 334.04; 304.45 mg/g. The results percentage RUDP are 27.91; 27.96; 28.03; 27.36%. The variant test results showed that the protective soy pulp protein used mangrove tannin significantly decrease NH3 concentrations, but no significantly on total protein production and percentage RUDP. The DMRT test result showed that NH3 concentrations on T0 significantly higher (p<0.05) from T1, T2 and T3 while T1, T2 and T3 not significantly different (p>0.05). From the results of this research concluded that the protection of soy pulp protein used mangrove leaves tannin proven to decrease ammonia concentration (NH3) but did not affected to total protein production and percentage RUDP.Keywords: soy pulp; tannin; ammonia; total protein
Effect of SOYXYL as a Dietary Protected Protein Supplement on the Reproductive Performance of Simmental Bulls Prasetiyono, Bambang Waluyo Hadi Eko; Widiyanto, Widiyanto; Ondho, Yon Soepri; Subrata, Agung; Widodo, Hermawan Setyo
ANIMAL PRODUCTION Vol. 22 No. 1 (2020)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with Animal Scientist Society of Indonesia (ISPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2020.22.1.41

Abstract

The study aimed to evaluate the effect of SOYXYL as one of the dietary protected protein supplement product in the rations on the reproduction performance of Simmental bulls. Twelve 5 years-old Simmental bulls were allotted to 4 treatment groups with 3 replications in each, i.e.: T0= control ration (elephant grass + 5 kg/head/day commercial concentrate), T1= control ration + 150 g protected protein supplement SOYXYL (3% of concentrate), T2 = control ration + 300 g SOYXYL (6% of concentrate) and T3= control ration + 450 g SOYXYL (9% of concentrate). The experiment lasted for 90 days. At the end of the experiment, semen and blood were collected and body weight gain was measured. The variables measured included sperm motility, sperm concentration, blood protein, blood urea and the concentration of testosterone. Results showed that SOYXYL supplementation, particularly at the levels of 3 and 6%, increased the total plasma protein of bulls. SOYXYL supplementation at 3% from concentrate resulted in increased plasma urea concentration. Feeding SOYXYL, particularly at the levels of 6 and 9%, increased the average daily gain of bulls as compared to control. Feeding 3% SOYXYL increased, but at the levels of 6 and 9% decreased the concentration of sperm of bulls. The concentration of testosterone was higher in the bulls received 3 and 6% SOYXYL in the rations. Dietary supplementation of SOYXYL increased the sperm motility of Simmental bulls. In conclusion, dietary supplementation of SOYXYL at the level of 3% increased plasma protein, testosterone, and sperm concentrations as well as sperm motility. However, supplementation of SOYXYL at 6 and 9% from rations decreased the concentrations of sperm and testosterone.
The Effect of Choline Chloride Supplementation on the Reproductive Performance of Simmental Bulls Fed Protected Protein in the Ration Bambang Waluyo Hadi Eko Prasetiyono; Yon Soepri Ondho; Agung Subrata; Pramesti Kusuma Pratiwi; Mutiara Briliana Zahra; Trisna Itmamulwafa; Tertyani Kartika Pratiwi; Muslichatun Nisa; Widiyanto Widiyanto
Buletin Peternakan Vol 44, No 2 (2020): BULETIN PETERNAKAN VOL. 44 (2) MAY 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i2.55338

Abstract

SOYXYL supplementation as a source of high-quality protected protein in rumen (Rumen Protected Protein) has proven to improve reproduction performance of bulls. The SOYXYL is an extrusion product of soybean (Glycine max) as a source of rumen protected protein. The potential of the protein supplementation is maximally expressed when it is supported by sufficient of metabolic rate. In this case the choline chloride supplementation increases the metabolic rate. The aim of the research was to analyze the effect of choline chloride supplementation on reproduction performance and hematologic status related to reproduction performance of bulls fed protected protein in the ration. The materials used were 12 bulls aged 5 years having weight of 700 ± 2.75 kg that were divided into 4 treatment groups, each of which consisted of 3 heads. The treatments applied were T0 (control): ration (grass + commercial concentrate) + protected protein supplement “SOYXYL”; T1: control + choline chloride 0.145% dry matter (DM) concentrate/head/day; T2: control + choline chloride 0.290% DM concentrate/head/day; T3: control + choline chloride 0.435% DM concentrate/head/day. Variables measured were nutrient consumption, blood lipid status, blood protein status, and reproduction performance. Data were analyzed using ANOVA in a completely randomized design and statistically processed using SAS program. The result showed that choline chloride supplementation by 0.435% from DM concentrate increased (p<0.05) the concentration of blood plasma protein, blood testosterone hormone, sperm concentration, and sperm motility from 6.44 g/dL, 4.66 ng/mL, 1006.74 million/mL, and 36.00%, respectively, in T0 to be 8.57 g/dL, 9.07 ng/ml, 1270.41 million/mL, and 70.00% in T3, respectively. The choline chloride supplementation up to 0.435% from DM concentrate to bulls supplemented with protected protein containing in the ration increased reproduction performance.
Evaluasi Fisik Organoleptik Multinutrien Blok yang dibuat dengan Metode Panas pada Penambahan Level Molases yang Berbeda Dyah Ayu Putri; Retno Iswarin Pujaningsih; Agung Subrata
Bulletin of Applied Animal Research Vol 2 No 1 (2020): Bulletin of Applied Animal Research
Publisher : LPPM Perjuangan University of Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36423/baar.v2i1.224

Abstract

Penelitian bertujuan untuk mengevaluasi pengaruh penambahan level molases terhadap kualitas fisik dan organoleptik pada Multinutrien Blok (MnB) yang dibuat dengan metode panas. Materi yang digunakan dalam penelitian adalah molases, hijauan jagung, garam, cangkang kerang darah, cangkang telur, urea dan bentonit. Alat yang digunakan yaitu chopper, disc mill, palu, kompor, panci, ember, cetakan MNB, timbangan digital dan kuesioner. Rancangan percobaan yang digunakan yaitu rancangan acak lengkap dengan 4 perlakuan dan 4 ulangan (T0 : tanpa penambahan molases (kontrol), T1 : penambahan molases sebanyak 20%, T2 : penambahan molases sebanyak 35% dan T3 : penambahan molases sebanyak 50%). Parameter yang diamati yaitu kualitas fisik (kadar air) dan kualitas organoleptik (warna, tekstur dan aroma) pada Multinutrien Blok. Data analisis menggunakan analisis ragam dan dilanjutkan dengan uji lanjut wilayah ganda Duncan. Hasil penelitian menunjukkan bahwa penambahan level molases berpengaruh nyata (P<0,05) terhadap warna, tekstur dan aroma. Perlakuan T3 pada pengujian warna, tekstur dan aroma memiliki nilai paling tinggi yaitu 3,99; 3,55 dan 3,32. Kadar air menunjukan tidak berpengaruh nyata. Simpulan dari penelitian ini adalah penambahan level molases sebesar 50% menghasilkan kualitas fisik dan organoleptik paling baik pada pembuatan Multinutrien Blok dengan metode panas.
The Effect Of Duration Time On Onggok Fermentation With Enrichment N, S, P By Trichoderma Reesei On Nutrien Value Nila Cahyaning Febriyani; Agung Subrata; Surahmanto Surahmanto; Joelal Achmadi
Bulletin of Applied Animal Research Vol 2 No 1 (2020): Bulletin of Applied Animal Research
Publisher : LPPM Perjuangan University of Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36423/baar.v2i1.226

Abstract

The aim of this research was to study the effect of incubation time on onggok fermentation with enrichment N, S, P by Trichoderma reesei on nutrient value. The materials of this research were onggok, T. Reesei and N, S, P element. This research used completely randomized design with 4 treatments different incubation time with 5 replication; T0: 0 day, T1: 2 days, T2: 4 days and T3: 6 days. The parameters observed were crude protein, crude fat, crude fiber, and nitrogen free extract of fermented onggok. Data analysis was used by Analysis of Variance (ANOVA) and continued by Duncan’s Multiple Range test if they were significantly different. Results of this research showed that there were haven’t effect (P>0.05) of the treatment on nutrient value. The conclusion of this research was incubation time on onggok fermentation with enrichment N, S, P by Trichoderma reesei have not been able to decrease crude fiber.Keywords : Onggok, fermentation, T.reesei
Kecernaan dan Fermentabilitas Ruminal In Vitro Onggok yang Difermentasi Trichoderma reesei dengan Suplementasi N, S dan P Shibghatullah Dina Muhammad; Agung Subrata; Surahmanto Surahmanto; Joelal Achmadi
Bulletin of Applied Animal Research Vol 2 No 2 (2020): Bulletin of Applied Animal Research
Publisher : LPPM Perjuangan University of Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36423/baar.v2i2.263

Abstract

Penelitian ini bertujuan untuk mengkaji kecernaan dan fermentabilitas ruminal in vitro onggok yang difermentasi Trichoderma reesei (T. reesei) dengan suplementasi N, S dan P. Materi yang digunakan dalam penelitian ini adalah onggok yang telah disuplementasi N, S dan P dengan rasio 13:1:1 serta starter T. reesei sebanyak 1,5% dari BK substrat. Rancangan percobaan yang digunakan adalah rancangan acak lengkap yang terdiri dari 4 perlakuan dengan 5 ulangan berupa T0 (lama fermentasi 0 hari), T1 (lama fermentasi 2 hari), T2 (lama fermentasi 4 hari) dan T3 (lama fermentasi 6 hari). Parameter yang diamati meliputi kecernaan bahan kering (KcBK), kecernaan bahan organik (KcBO), konsentrasi volatile fatty acids (VFA) dan amonia (NH3). Data dianalisis menggunakan analisis ragam taraf 5%, apabila ada pengaruh yang nyata maka dilanjutkan dengan uji Duncan’s multiple range test. Hasil penelitian menunjukkan bahwa lama fermentasi dapat meningkatkan (P<0,05) KcBK, KcBO dan konsentrasi VFA, akan tetapi menurunkan (P<0,05) konsentrasi NH3 onggok. Simpulan yang diperoleh yaitu lama fermentasi dapat meningkatkan kecernaan dan konsentrasi VFA akan tetapi menurunkan konsentrasi NH3 onggok.Kata kunci: lama fermentasi, suplementasi mineral, onggok, in vitro
The Interactive Effect between Fermentation with Trichoderma reesei on Ammoniation on Fiber Component of Sugar Palm Pulp Nurul Husnah Febrianti; Agung Subrata; Joelal Achmadi
Bulletin of Applied Animal Research Vol 2 No 2 (2020): Bulletin of Applied Animal Research
Publisher : LPPM Perjuangan University of Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36423/baar.v2i2.468

Abstract

The purpose of this research was to examine the combination effect of fermentation time by Trichoderma reesei and ammoniation on fiber component of sugar palm pulp. The research used sugar palm pulp from Boyolali which was sun-dried. Sugar palm pulp fermentation with Trichoderma reesei requires nitrogen for microbial growth. Ammoniation process could loose lignocellulosic bonds and provides the nitrogen supply for Trichoderma reesei, therefore these treatments could optimize Trichoderma reesei in the fermentation process. Samples were fermented for 0, 3 and 6 days with 1,5% Trichoderma reesei. The research treatments were allocated according to a completely randomize design with  pattern of 2x3 factorial. First factor was ammoniation treatment and second factor was variation of fermentation time. The parameters observedd were NDF, ADF, hemicellulose, cellulose and lignin. Research result showed that there is was no interactive effect between treatments on reducing levels of NDF and ADF, but interaction treatments decreased (P<0.05) levels of, cellulose and lignin. In conclusion, the interactive effet between fermentation time with Trichoderma reesei and ammoniation reduce levels of hemicellulose, cellulose and lignin in sugar palm pulp.