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All Journal Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
M.Y. Probohoesodo
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Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience

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EVALUASI PEMERIKSAAN IMUNOKROMATOGRAFI UNTUK MENDETEKSI ANTIBODI IgM DAN IgG DEMAM BERDARAH DENGUE ANAK Ety Retno Setyowati; Aryati Aryati; Prihatini Prihatini; M.Y. Probohoesodo
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 12, No 2 (2005)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v12i2.850

Abstract

The gold standard diagnosis of DHF by RT-PCR needs a complex technology and is time consuming. Serological tests have beendeveloped to detect IgM and IgG anti dengue to determine primary as well as secondary acute phase infection. IgM and IgG antidenguetests by immunochromatography have been used, due to a high diagnostic validity, also because they are simple, practicable, easy, rapid(15–30 minutes), can be used in a single serum sample. ELISA method has been used as a confirmation method. The aim of this studyis to evaluate the immunochromatography method in detecting IgG and IgM anti dengue of DHF patients. The study was performedon 50 serum samples from patients of the ICU Department of Paediatrics Dr. Soetomo Hospital, Surabaya during July–August 2005with dengue virus infection according to the 1997, WHO criterion and 27 serum samples from non dengue virus infection patients.ELISA method showed positive infection in 44 samples. Immunochromatography method showed positive infection in 43 samples, butwas negative in 1 sample. Diagnostic sensitivity of Immunochromatography is 97.7% (43/44) and the diagnostic specificity is 92.6%(25/27). Immunochromatography method has a high diagnostic value in assisting the diagnosis of DHF.
POLA MIKROORGANISME PADA LIANG VAGINA WANITA HAMIL DI RSU DR. SOETOMO SURABAYA Sianny Herawati; M.Y. Probohoesodo; Prihatini .
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 12, No 2 (2005)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v12i2.846

Abstract

In pregnant women especially in the third trimester, it is seldom that attention is paid to microorganism in the vagina. Many studies have reported about microorganism in the vagina which possibly can infect the foetus. This is due to the migration of bacterial colonization in the vagina to the foetus during labour, causing infection or sepsis in the foetus after delivery. To study the microorganism pattern in the vagina of pregnant women, pathogenic as well as normal flora of the vagina. If the microorganism is known, prevention or treatment before and after delivery can be applied. Population was recruited from pregnant women in the third trimester who visited The Maternity Outpatient Clinic, Dr. Soetomo Hospital from September until October 2005. Specimens from ostium of the cervix and posterior fornix of the vagina were collected in Amies media and examined in the Department of Clinical Pathology, Airlangga University/ Dr. Soetomo Hospital to detect any bacteria, fungi and parasites using direct method and culture. The microorganism pattern from 30 pregnant women consisted of non-haemolytic Streptococcus 25%, Escherichia coli 25%, Lactobacillus 35%, Staphylococcus aureus 10%, and Staphylococcus coagulase negative 5%. The result of fungi examination showed 14 (47%) samples with negative result and 16 (53%) samples with positive result (Candida sp). The pattern of microorganism was dominated by Lactobacillus and Candida sp but no parasites in the vagina were found.
PENEMUAN (DETEKSI) ANTIBODI UNTUK ANTIGEN TUBERKULOSIS MENGGUNAKAN METODE IMUNOKROMATOGRAFI DI PENDERITA TUBERKULOSIS PARU Kadek Mulyantari; Aryati .; M.Y. Probohoesodo
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 14, No 3 (2008)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v14i3.938

Abstract

The gold standard for TB still has some drawbacks, such as a long duration for culture examination and the rolated facilities are notalways available in all laboratories. One of methods in diagnosing tuberculosis infection is by immunochromatography (ICT). MYCOTECTB xp (recombinant) is one of serologic tests using immunochromatography principle. MYCOTEC TB xp uses recombinant antigens 38kDa, 16 kDa, 6 kDa and Early Secreted Antigen Target (ESAT-6). This method is expected so far diagnose TB in a short time and has ahigh accuracy. Evaluating the immunochromatography method in detecting antibody by tuberculosis antigen in lung TB patients as willthose with nonTB lung disease (lung tumor, bronchial asthma, pneumonia, chronic obstructive lungdisease). Serum samples of 30 TBpatients in BP4/Karang Tembok Hospital Surabaya and 30 non TB patients in the Dr. Soetomo Hospital Surabaya. Detection of antibodyto tuberculosis antigen was done with MYCOTEC TB xp. In this study found is prond 30 TB patients using MYCOTEC TB xp was positivein 23 samples and negative in 7 samples. From the 30 nonTB patients MYCOTEC TB xp was positive in 4 samples and negative in 26samples. It can be uncloaded so far that the diagnostic sensitivity of MYCOTEC TB xp was 76.7% (23/30) and diagnostic specificity was86.7% (26/30). MYCOTEC TB xp has an intermediate diagnostic sensitivity of 76.7% and a high diagnostic specificity of 86.7%.
PROKALSITONIN SEBAGAI PENANDA PEMBEDA INFEKSI BAKTERI DAN NON BAKTERI Bastiana Bastiana; Aryati Aryati; Dominicus Husada; M.Y. Probohoesodo
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 2 (2011)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i2.1019

Abstract

Early diagnosis of an infection and prompt administration of an antibiotic can dramatically reduce morbidity and mortality.Procalcitonin (PCT), a precursor of calcitonin, has been proposed as a marker of bacterial infection. The aim of this study is to assess theefficiency of procalcitonin in children for the diagnosis of bacterial vs. non bacterial infection. This was a prospective, cross-sectional study.The subjects were enrolled consecutively, consisting of feverish children (temperature ³38.5° C) admitted to the Pediatric EmergencyDepartment with ages up to 12 years old. The subjects were divided into two groups according to their final diagnosis, bacterial and nonbacterial infection. Serum PCT concentration was measured by enzyme linked fluorescent assay (ELFA) method. Sensitivity, specificity,positive predictive and negative predictive values, and receiver operating curve (ROC) of PCT were calculated. Out of 54 patients,24 (44.4%) had a final diagnosis of bacterial infection. PCT showed a wide concentration range in the bacterial infection group (median:1.09 ng/mL, lower (L)=0.05 ng/mL, upper (U)=128.7 ng/mL) compared with non bacterial infection group (0.21 ng/mL; L=0.05ng/mL; U=12.15 ng/mL). There was a significant difference in PCT between the 2 groups (p=0.020). ROC analysis demonstrated anarea under curve (AUC) of 0.686 (95% CI, 0.534 to 0.838). Using a cut-off point of 0.5 ng/mL, the sensitivity, and specificity, positivepredictive and negative predictive values of PCT were 66.7%, 76.7%, 69.6%, 74.2%, respectively. In this study, PCT may be useful fordifferentiation of bacterial vs. non bacterial infection in children.
Co-Authors aryati . Aryati Aryati Bastiana Bermawi Dominicus Husada Ety Retno Setyowati Ni Kadek Mulyantari Prihatini . Prihatini Prihatini Sianny Herawati