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UJI CEPAT (RAPID TEST) ANTIBODI IgM TERHADAP Salmonella typhi DEMAM TIFOID Rini Riyant; Prihatini .; Siti Rochmatoen
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 14, No 3 (2008)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v14i3.935

Typhoid fever is caused by Salmonella typhi. The definitive diagnosis can be made by isolation of Salmonella typhi from blood, bone marrow or other body fluids. To support the clinical diagnosis of typhoid fever in Indonesia, where most hospitals and health centres haveno facilities for cultures, a rapid test for the detection of lipopolysaccharides (LPS) Salmonella typhi-specific IgM antibodies was evaluatedon serum samples from patients with typhoid fever. This study is proposed to know the rapid test diagnostic value for the detection oflipopolysaccharides (LPS) Salmonella typhi-specific IgM antibodies. A cross sectional, observational analytical study on 27 typhoidfever and 25 dengue hemorrhagic fever patients of the Dr. Soetomo Hospital, Dr. M Soewandhi General Hospital and Gotong-RoyongClinic has been conducted from January – May 2007. The diagnosis of typhoid fever patients was based on positive gall culture whilethe diagnosis of dengue hemorrhagic fever was based on negative gall culture, positive serology examination for dengue hemorrhagicfever and a recovery from dengue hemorrhagic fever with standard treatment. The sera from patients were examined using a rapid testfor the detection of lipopolysacharides (LPS) Salmonella typhi specific IgM antibodies from Amgenix Onsight of the first blood samples(collected on admission to the hospital) the rapid test for IgM antibodies showed the following: sensitivity 70.4%, specificity 80.0%,positive predictive value 79.2%, negative predictive value 71.4%, diagnostic efficiency 75% respectively. Of the second blood samples(collected 2–3 weeks during the illness) therapid test for IgM antibodies showed the following: sensitivity 88.9%, positive predictive value 82.8%, negative predictive value 87.0%, and diagnostic efficiency 84.6% respectively. The rapid test for IgM antibodies has a high diagnostic value for typhoid fever. The assay uses stabilized components which can be stored at room temperature; the test does notrequire special equipment and may be used in health centres that have no facilities for culture.

DIAGNOSIS FILARIASIS BERDASAR HAPUSAN DARAH TEPI H. I. Malewa; Prihatini .
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 15, No 1 (2008)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v15i1.951

Filariasis is a disease group affecting humans and animals caused by nematode parasites of the order Filariidae, commonly calledfilariae. Filarial parasites may be classified according to the habitat of the adult worms in the vertebral host. the lymphatic groupincludes Wucheria bancrofti, Brugia malayi and Brugia timori. the cutaneous group includes Loa-loa, onchocerca volvulus, andMansonella streptocerca. the body cavity group includes Mansonella perstans and Mansonella ozzardi. these parasites are transmittedto humans through the bite of an infected mosquito. Clinical findings vary from asymptomatic until severe, depending on geography,parasite species, immune response and intensity of the infection A 19-years-old Javanese woman, presented with oedema on right legs.She has been suffering from oedema on right leg since she was 7-years-old. Physical examination showed elephantiasis on the right leg.Early, the patient had swelling in right inguinal area spreading into her right leg. the oedema was persistent and become larger untilnow. there was no fever and pain. She had history living in South Borneo when she was 1 until 4 years old. She did not receive anymedication before. Laboratory examination showed no abnormality both of complete blood count and clinical chemistry. From thin bloodsmear examination many forms of microfilariae were found. the data showed manifestation of filariasis in the blood.

POLA MIKROORGANISME PADA LIANG VAGINA WANITA HAMIL DI RSU DR. SOETOMO SURABAYA Sianny Herawati; M.Y. Probohoesodo; Prihatini .
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 12, No 2 (2005)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v12i2.846

In pregnant women especially in the third trimester, it is seldom that attention is paid to microorganism in the vagina. Many studies have reported about microorganism in the vagina which possibly can infect the foetus. This is due to the migration of bacterial colonization in the vagina to the foetus during labour, causing infection or sepsis in the foetus after delivery. To study the microorganism pattern in the vagina of pregnant women, pathogenic as well as normal flora of the vagina. If the microorganism is known, prevention or treatment before and after delivery can be applied. Population was recruited from pregnant women in the third trimester who visited The Maternity Outpatient Clinic, Dr. Soetomo Hospital from September until October 2005. Specimens from ostium of the cervix and posterior fornix of the vagina were collected in Amies media and examined in the Department of Clinical Pathology, Airlangga University/ Dr. Soetomo Hospital to detect any bacteria, fungi and parasites using direct method and culture. The microorganism pattern from 30 pregnant women consisted of non-haemolytic Streptococcus 25%, Escherichia coli 25%, Lactobacillus 35%, Staphylococcus aureus 10%, and Staphylococcus coagulase negative 5%. The result of fungi examination showed 14 (47%) samples with negative result and 16 (53%) samples with positive result (Candida sp). The pattern of microorganism was dominated by Lactobacillus and Candida sp but no parasites in the vagina were found.

KEGUNAAN SISTEM PENGOTOMATAN (OTOMASI) LABORATORIUM/ LAS (LABORATORY AUTOMATION SYSTEMS) Prihatini .
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 15, No 1 (2008)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v15i1.952

In most laboratory LAS (Laboratory Automation System) system recently have been used. though, not all of them used theautomation system and LIS.the LAS is used for the diagnosis of diseases, because it can decrease the error factors as weel as thelaboratoric examination. Regarding to decreasing problems, the expenses of patients who staying in the hospitals could be reduced aswell as their time to stay. the purpose of this article is to know comprehensively LAS and its services in the future in the hospitals' clinicallaboratory. Because before LAS was used the diagnosis time of diseases take a long time as compared to LAS.

NILAI DIAGNOSTIK MALARIA ANTIGEN CASSETTE PENYAKIT MALARIA Binawati .; Prihatini .; M.Y Probohoesodo
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 1 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i1.1031

Malaria is an endemic disease in many countries. In 103 endemic countries with around 2.5 billion population, 1−3 million death cases were reported every year. Clinical criteria and blood smear established the diagnosis of malaria. ICT (imuno chromatographytest) is needed in peripheral areas where there are no experienced laboratory technicians. The procedure is simple, practical, easy, aswell as quicker than the conventional method, and no experienced technicians are needed this ICT advantages. The aim of this study isto know the diagnostic value of Malaria Antigen Cassette with microscopic examination as the gold standard examination of malaria.This research used observational cross-sectional method This study was done in Nusa Tenggara Barat during May−June 2008. Bloodsamples were taken by finger prick in patients with Malaria symptoms: fever, chill and sweating, followed by examining the blood smearby Malaria Antigen Cassette from Focus Diagnostic. The diagnostic value was then evaluated by calculating the sensitivity, specificity,positive predictive value as well as the negative predictive value. The diagnostic value of Malaria Antigen Cassette in patients withmalaria falciparum was found as follows: sensitivity 95.2%, specificity 100%, positive predictive value 100%, and negative predictivevalue 97.6%. The diagnostic value of Malaria Antigen Cassette in patients other types than malaria falciparum was as follows: sensitivity94.8%, specificity 100%, positive predictive value 100%, and negative predictive value 95.3%. The diagnostic value of Malaria AntigenCassette was very high in diagnosing malaria falciparum compared to other types than malaria falciparum.

MEMBANGUN SENDIRI SISTEM INFORMASI LABORATORIUM Yogi Sucahyo; Supri .; Prihatini .
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 14, No 3 (2008)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v14i3.939

LIS (Laboratory Information System) include software, hardware and brain ware. It could accepted the laboratoryinformation’s,analyze and organize the activity clinical laboratory The laboratory information system confirm laboratory’s businessfrom input and output process According this Semen Gresik Hospital was increase the quality of clinical laboratory information. TheLIS needs computers as workstations, instrument interface server, as well as laboratory automation systems. The recorded ID barcodelabels used for patients identification as well as for administration and labeling the samples. The samples were process (analyses) andthe results were printout. LIS will decrease the patients’ turn around time as well as the praeanalytic errors. The self-building of LIS ischeaper than the laboratory instruments bought including.

PENYEBARAN GUMPALAN DALAM PEMBULUH DARAH (DISSEMINATED INTRAVASCULAR COAGULATION) AKIBAT RACUN GIGITAN ULAR Prihatini .; Trisnaningsih .; muchdor .; U N Rachman
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 14, No 1 (2007)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v14i1.923

Cases of snake bites were seldom happened in town. From the 2500–3000 world-wide distributed species of snakes, 500 are venomous. The snake produce some toxic substance which is dangerous in men , and could cause morbidity or mortality. It’s caused byophitoxaemia, which influence the permeability of capilers with consequence bleeding. There patients must be examined physically, localas well systemic. The laboratoric examinations were based on clinical symptoms, by determination of the snake venom causal and thesequalae in the human body, including coagulopathy, anemia as well as renal failure, etc. The snake venom may cause necrotic tissue offoot and anemia by trombocytopenia.The condition of this victim patient was severre due to his diabetic syndrome. This article presenteda study of snake bite incident on an old man with DIC laboratoric results, following anemia and renal failure which caused his death.

IDENTIFIKASI CEPAT MIKROORGANISME MENGGUNAKAN ALAT VItEK-2 prihatini .; aryati .; hetty .
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 13, No 3 (2007)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v13i3.915

Vitek-2 is automatic microbiologic equipment which used to identify antimicrobial sensitivity by micro organism test have been launched in Indonesia. In some private laboratory and hospital this equipment has been used already. The purpose of this study is toknow how to operate this equipment to ID and AST as well as to compare with the conventional methods. The material used to test withthis equipment consist of pure colony culture which derived by suspension of one inoculation to ID or AST card. The reagent consistsonly of saline suspension. After the suspension installed to VITEK2, the result was verified in the printout about 1.5 hours. The colour ofID and AST is difference and couldn’t change with each other. The results are appear more rapid than the others conventional methods.VITEK-2 can be used as a means to diagnose faster than the conventional methods., so that the physician could prescribe earlier as soonas possible to patients and reduced the hospital budgets as well.

TROMBOSIT ABNORMAL PASCAPERSALINAN Prihatini .; S. Hadi; Wijanda H T Sylvaranto; Maksum .
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 1 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i1.1029

Pregnant women with cardio vascular failure caused by hypertension may show pre-eclampsia or eclampsia syndrome. The syndromewas confirmed by the hypertension family history when she was pregnant or during labour formerly. The influence was shown in thehaematological examination, besides inducing hypertension during pregnancy. The examination must be an accurate laboratory procedureto avoid complication. The routine obstetric examination should be a physical, laboratory as well as USG one. Exspecially laboratoryexamination for pre-eclampsic and eclampsic patients like clinical chemistry, blood examination, urinalysis and special examinationas ANA, ACA and TAT The result of laboratoric examination showed anemic,trombosits was normal and TAT abnormal. The baby wasborn normal. After 3 months post labour the result of TAT still hyper-reactive especially for ADP agonist and hypo-reactive for collagenagonist. This patient have a trombosit function failure based on TAT and must be care to cardiovascular disease. Pregnant women withpre-eclamptic or eclamptic risk must be monitored the trombosit function beside clinical laboratoric and obstetric examinations

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