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All Journal Indonesian Journal of Chemistry Berkala Penelitian Hayati
Nuniek Herdyastuti
Department of Chemistry, Surabaya State University, Jl. Ketintang Surabaya 60231
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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CHITINOLYTIC BACTERY ACTIVITY ISOLATED FROM THE MUD FIELDS Nuniek Herdyastuti; Sari Edi Cahyaningrum; Tri Joko Raharjo; Mudasir; Sabirin Matsjeh
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 2 (2010): June 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/262

Abstract

A chitinolytic microorganism had been isolated from mud fields in Ketintang Surabaya area by using a minimal media containing 0.4% colloidal chitin. Activity assay is based on released N-acetyl-glucosamine which reacted with 3,5 dinitrosalicyclic acid. Mud fields produce 63 bacterial isolates with chitinase activity (TNH1 – TNH63 isolates). The highest activity was shown by TNH11 isolate with specific activity 1.27 U/mg. TNH11 isolates was Gram negative, rod-cocoid cell, has a colony of yellow, round shape, convex elevation, flat margin and the colony diameter 3–5 mm.
CHITINASE AND CHITINOLYTIC MICROORGANISM : ISOLATION, CHARACTERIZATION AND POTENTIAL Nuniek Herdyastuti; Tri Joko Raharjo; Mudasir Mudasir; Sabirin Matsjeh
Indonesian Journal of Chemistry Vol 9, No 1 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2407.494 KB) | DOI: 10.22146/ijc.21580

Abstract

Chitinase is enzyme that hydrolyzes chitin, a polimer of b-1,4-N-Acetilglucosamine which is the most abundant natural resource after cellulose. Chitinolytic microorganism can be found in  environment like soil and water that contain chitin, or in extreme environment which is known as thermofilic microorganism. Chitinolytic microorganism is identified by recognizing the morphological and physiological properties based on Bergey's Manual of Systematic Bacteriology. The sequence data of the 16S rRNA genes is determinated in the GeneBank nucleotide sequence database. Chitinase activity can be qualitatively determined from the clearance zone around the colony formed in agar medium containing colloidal chitin. Chitinase can be utilized as biocontrol agent and derivate chitin as the result of chitinase degradation which can be used in the fieltd of health, food, industry and waste management
Co-Authors Mudasir Mudasir Mudasir Sabirin Matsjeh Sari Edi Cahyaningrum Tri Joko Raharjo