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All Journal Indonesian Journal of Cancer Chemoprevention Journal of Fundamental and Applied Pharmaceutical Science
Laras Widawaty Putri
Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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Antiproliferative Activity of Ethanolic Extract of Ciplukan Herbs (Physalis angulata L.) on 7,12-Dimethylbenz[a]nthracene-Induced Rat Mammary Carcinogenesis Putri, Laras Widawaty; Ikhtiarsyah, Yurista Gilang; Meiyanto, Edy; Monikawati, Ameilinda; Farida, Sofa
Indonesian Journal of Cancer Chemoprevention Vol 2, No 2 (2011)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Physalis angulata  L.  is  an  annual  herb  widely  used  as  popular  medicine  for  the treatment of cancer. Physalis angulata L. ethanolic extract (PEE) has been demonstrated to have strong cytotoxic activity against breast cancer, inhibited cancer cell’s proliferation and induced  cell  cycle  arrest.  The  aim  of  our  study  is  to  investigate  the  effect  of  PEE  as  a cancer  chemopreventive  agent  on  7,12-dimethylbenz[a]nthracene  (DMBA)-induced  rats mammary. The antiproliferative activity was characterized by monitoring the histopatology representation  and  expression  of  cell  proliferation  on  DMBA-induced  mammary  rats  that were  treated  with  PEE  against  control  groups.  The  histopatology  representation  were analyzed  by  Haematoksilin  Eosin  (HE)  staining  method,  while  proliferative  activity  was detected by AgNOR method. The HE staining results showed significant differences in cells morphology  of  treatment  groups  compared  to  the  control  groups.  Thus  results  suggest that  PEE  was  able  to  repair  morphology  of  cells  undergoing  carcinogenesis.  AgNOR method  showed  decreasing  occurrence  of  black  dots  between  treatment  and  control groups. Thus, we conclude that PEE has an antiproliferative activity on DMBA-induced rat mammary.  Therefore,  the  ethanolic  extract  of  Physalis  angulata  herbs  is  a  potential chemopreventive agent on cancer. Further study on its molecular mechanism needs to be explored. Keywords:  Physalis  angulata,  breast  cancer, 7,12-dimethylbenz[a]nthracene, carcinogenesis, antiproliferative
Evaluation of the Antithrombotic Activity of Acmella oleracea L. Flower Ethanol Extract Tiaravista, Amanda Gita; Putri, Laras Widawaty; Nurrochmad, Arief; Widyarini, Sitarina; Fakhrudin, Nanang
Journal of Fundamental and Applied Pharmaceutical Science Vol 4, No 1 (2023): August
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/jfaps.v4i1.19055

Abstract

Acmella oleracea L. (hereinafter abbreviated as AOE) is one of the plants with the potential for antithrombosis, one of the deadliest cardiovascular diseases in Indonesia. The antithrombotic activity test on AOE leaf extract revealed that it could lyse blood clots. However, no information regarding the AOE flower as an antithrombosis is provided. This study, thus, aims to determine the chromatography profile and the antithrombotic activity of the AOE flower ethanol extract. To identify the AOE chemical profile, thin-layer chromatography was carried out. Antithrombotic testing was performed on male rats of the Sprague-Dawley strain. Then, the antithrombotic activity was tested using the FeCl3-induced rat method, with the observed parameter being total occlusion time. The test animals were also divided into six groups: normal, solvent (CMC-Na 0.9%), comparator drug (clopidogrel 8.67 mg/kg), and AOE (doses 125, 250, and 375 mg/kg). The data obtained were then analyzed statistically using Kruskal-Wallis, followed by Tukey's. The TLC profile results confirmed the presence of the alkaloid compound in AOE. The authors also found that AOE at doses of 125, 250, and 375 mg/kg significantly prolonged the occlusion time comparable to that of clopidogrel at 8.67 mg/kg (p 0.05). This finding indicates that AOE has antithrombotic activity in FeCl3-induced rats.
Co-Authors Ameilinda Monikawati Arief Nurrochmad Edy Meiyanto Nanang Fakhrudin, Nanang Sitarina Widyarini Sofa Farida Tiaravista, Amanda Gita Yurista Gilang Ikhtiarsyah