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All Journal HAYATI Journal of Biosciences BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Mutu Pangan : Indonesian Journal of Food Quality
Winiati P. Rahayu
Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Immunology & microbiology Public Health Veterinary

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Phenotypic and Genotypic Antimicrobial Resistance Profile of Salmonella spp. Isolated from Kampung Chicken Carcasses Nurjanah, Siti; Rahayu, Winiati P.; Sanjaya, Stephen
HAYATI Journal of Biosciences Vol. 31 No. 2 (2024): March 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.2.229-240

Abstract

Kampung chicken meats have been widely consumed in Indonesia as well as broiler chicken. However, the extensive rearing allowed multidrug-resistant (MDR) bacteria exposure to kampung chicken, including through horizontal gene transfer. This study aimed to observe the correlation between the phenotypic and genotypic antimicrobial resistance profile of Salmonella spp. isolated from kampung chicken carcasses. Phenotypic antimicrobial resistance was evaluated by Kirby-Bauer's disk diffusion method, while the detection of drug resistance genes in seventeen isolates of Salmonella was carried out by PCR. All (17/17) isolates were susceptible to ampicillin and chloramphenicol. Most isolates of Salmonella were resistant to erythromycin (82%; 14/17), while the decreased susceptibility (intermediate category) most occurred in oxytetracycline (82%; 14/17). Salmonella Typhimurium showed a resistance pattern to more antimicrobial groups than S. Newport and S. Weltevreden. Several antimicrobial resistance genes (blaTEM, tetG, cmlA, gyrA) were present in all (17/17) isolates of Salmonella spp. Resistance to antimicrobial agents and the presence of resistance genes were not always related. This study could provide beneficial information regarding the transmission of antimicrobial resistance among Salmonella spp. from kampung chickens.
Implementasi Kebijakan SPP-IRT oleh Dinas Kesehatan Kabupaten/Kota di Provinsi Jawa Barat Dewi, Retno Anggrina Khalistha; Tio, Yohana Tania; Nuraida, Lilis; Rahayu, Winiati P.
Jurnal Mutu Pangan : Indonesian Journal of Food Quality Vol. 12 No. 2 (2025): Jurnal Mutu Pangan
Publisher : Department of Food Science and Technology (ITP), Faculty of Agricultural Technology, Bogor Agricultural University (IPB) in collaboration with the Indonesian Food and Beverage Association (GAPMMI), the National Agency of Drug and Food Control, and th

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jmpi.2025.12.2.198

Abstract

The Certificate of Fulfillment of Home Industry Processed Food Production Commitments (SPP-IRT) is a legal authorization granted to home industries (IRTP) to produce and distribute processed food products (P-IRT). This study aimed to evaluate the suitability of SPP-IRT issuance in West Java Province and to identify the obstacles encountered in its implementation across different regions. The research was conducted using survey data from 17 respondents, each representing a District/City Health Office in West Java. The level of implementation was categorized into four groups—appropriate, moderately appropriate, less appropriate, and not appropriate—based on scores for main and additional criteria. The District/City Health Offices in West Java achieved 96.47% compliance with the main monitoring criteria and 94.12% compliance with the additional criteria. Fourteen health offices (82.35%) were categorized as appropriate, while three (17.65%) were categorized as less appropriate. The main obstacles reported by the District/City Health Offices included limited human resources, insufficient time, and inadequate budgets. Suggested solutions included increasing the number of certified food safety instructors and district food inspectors, improving time management and work prioritization, and optimizing budget allocation.
Persepsi dan Tantangan Sertifikasi Perikanan Berkelanjutan oleh Perusahaan Pengolahan Produk Perikanan Arsyad, Padma Rizki Dwi Arieanti; Rahayu, Winiati P.; Nurjanah, Siti
Jurnal Mutu Pangan : Indonesian Journal of Food Quality Vol. 12 No. 2 (2025): Jurnal Mutu Pangan
Publisher : Department of Food Science and Technology (ITP), Faculty of Agricultural Technology, Bogor Agricultural University (IPB) in collaboration with the Indonesian Food and Beverage Association (GAPMMI), the National Agency of Drug and Food Control, and th

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jmpi.2025.12.2.188

Abstract

The Sustainable Development Goals (SDGs) reflect a commitment to utilizing natural resources for human well-being. This includes efforts to promote a sustainable food system, including fishery products known as blue food. Sustainable aquaculture certification is important to ensure that products come from responsible sources, considering aspects of food safety, environment, animal welfare, and social responsibility. This study aims to assess the perception of companies towards the certification, including knowledge, attitudes, behaviors, and challenges in sustainable aquaculture certification. The survey was conducted on thirty respondents from aquaculture processing companies, divided into fifteen certified companies and fifteen uncertified companies. Certified companies showed good knowledge and attitude percentage scores (100%), and good behavior (93.3%). Meanwhile, uncertified companies had good knowledge (80%) and attitude (93.3%), but lower behavior (73.3%). This shows a behavioral gap in the uncertified group, even though they have a positive attitude towards sustainability certification. Significant correlations (p<0.05) between variables were identified between knowledge-behavior (rs=0.515), attitude-behavior (rs=0.639), attitude-challenge (rs=0.620), and behavior-challenge (rs=0.902). In uncertified companies, no significant correlation was found. This finding indicates that certified companies have a strong relationship between understanding, attitude, and behavior even though the companies are aware of the major challenges in implementation, while uncertified companies face structural or motivational barriers that hinder behavior even though they have a positive attitude. The main challenge faced by certified and uncertified companies is the limited knowledge of the team in implementation, with percentages of 40 and 46%, respectively. In addition, both groups of companies also face challenges related to the excessive cost of certification, which is recognized by 33% of each group.
MOLECULAR CHARACTERIZATION OF Aspergillus flavus TOXIGENICITY IN AGRICULTURAL COMMODITIES IN INDONESIA Anidah, Anidah; Rahayu, Winiati P.; Nurjannah, Siti; Retnowati, Ina
BIOTROPIA Vol. 30 No. 2 (2023): BIOTROPIA Vol. 30 No. 2 August 2023
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2023.30.2.1842

Abstract

Toxigenic Aspergillus flavus is a primary producer of aflatoxin in Indonesia, and its presence can lead to the contamination of agricultural commodities. This contamination poses a risk to export-targeted commodities, potentially resulting in their rejection. Therefore, this study aims to characterize the molecular profile of native A. flavus isolated from several Indonesian agricultural products, with a major focus on its toxigenicity and toxin production. A total of 18 A. flavus collections were isolated from nutmeg, ground peanut, cacao, coffee bean, corn, white pepper, and soil peanut plantation. Species identification was carried out using molecular and morphological approaches. The toxigenicity of isolates was characterized based on the amplification of aflatoxin gene clusters, while toxin production was assessed through growth simulation on a 10% coconut broth media followed by HPLC quantification. The result showed that all isolates were confirmed as A. flavus based on the morphological and sequence analysis of the ITS region. A total of 11 isolates (61%) were confirmed as toxigenic and produced 1-2 types of aflatoxin, in varying concentrations of high, moderate, or low levels of AFB1. High levels of AFB1 produced by seven isolates namely BIO3313, BIO33212, BIO3361, BIO33404, BIO3338, BIO3352, and BIO3344, had concentration levels ranging from 76.78 to 2241.06 µg/kg, while three isolates (BIO3314, BIO3312, and BIO3381) produced AFB1 below 1 µg/kg. Twenty-nine pairs of aflatoxin gene-specific sequences were successfully amplified as a single band, while some produced non-specific patterns in several low toxigenic and non-toxigenic isolates. Based on the results, it was concluded that completed gene clusters and variations of gene deletion were observed in both toxigenic and non-toxigenic isolates. However, no specific target gene could effectively distinguish the two groups. Two non-toxigenic isolates namely BIO3393 and BIO33403 exhibited a large deletion and could be potential candidates for biocontrol agents.
Co-Authors Anidah, Anidah Arsyad, Padma Rizki Dwi Arieanti Dewi, Retno Anggrina Khalistha Ina Retnowati Lilis Nuraida MADE ASTAWAN Ramadhani, Afrilia Sandra Sanjaya, Stephen Siti Nurjanah Siti Nurjannah Tio, Yohana Tania