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All Journal Buletin Peternakan JURNAL TEKNOLOGI LINGKUNGAN BERITA BIOLOGI JURNAL BIOLOGI INDONESIA
Maman Rahmansyah
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary Other

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OPTIMASI ENZIM ?-AMILASE DARI BACILLUS AMYLOLIQUEFACIENS O1 YANG DIINDUKSI SUBSTRAT DEDAK PADI DAN KARBOKSIMETILSELULOSA Soeka, Yati Sudaryati; Rahmansyah, Maman; ., Sulistiani
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i2.2200

Abstract

 ABSTRACTBacterial code O1 had been isolated from the leaven of fermented cassava. Based on molecular analysis by partial sequences of 16S rDNA and the phylogenetic character interpretation with Neighbor Joining Method, the strain was identified as Bacillus amyloliquefaciens O1. Bacterial enzymatic activity of ?-amylase was clarified due to the affect of temperature and pH, and as well as its enzymatic stability to convert 2% soluble starch in 100 ml standard media. Aim of the study was to provide benefit in regard on ?-amylase application as crude enzyme extract from the bacteria. In this study, the bacterial strain was being activated to produce ?-amylase by modifying substrates containing cassava starch, rice bran (RB), and carboxymethylcellulose (CMC) in five times volumes (500 mL) of the first scale setting in the standard media.  The result, reducing sugar as a result of enzymatic activity process increased 40 and 55 times in the modified media containing RB and CMC, respectively after 24 hours incubation. In the next 24 hours observation, enzyme activity in bacterial culture based on the RB media was able to degrade amylum in the muslin material containing amylum which was plunged in the media, 1.23 times higher compared to bacterial culture based on the CMC media. Media formula used in the study was able to induce extracellular enzyme activity as well as bacterial culture growth. Keywords: ?-amylase, Bacillus amiloliquefaciens, rice bran, carboxymethylcellulose 
PENGARUH INOKULASI BAKTERI TERHADAP PERTUMBUHAN AWAL JARAK PAGAR (JATROPHA CURCAS L.) Widawati, Sri; Rahmansyah, Maman
JURNAL BIOLOGI INDONESIA Vol 6, No 1 (2009): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i1.3175

Abstract

ABSTRACTBacterial inoculants affect the early growth of Jatropha (Jatropha curcas L). Genera ofAzotobacter, Bacillus, Chromobacterium, Citrobacter, Nitrosomonas, Rhizobium, andSpaerotillus natans were soil bacterial isolates. The soil was collected from numerous placesaround Pontianak, West Kalimantan. Those isolates were used as inoculants, and formulatedto single and mixed bacterial inoculants, then used to stimulate the early growth of jatrophaseedling in 15 weeks at greenhouse condition. Bacterial inoculations caused better growthperformance compared to its control as pure soil garden medium without inoculations, andneither to bare soil dresses with compost. In the presence of inoculants, plant height wasaccelerated quickly while other inoculants affected to stalk diameter development. Daily growthperformance of jatropha peaked in 8 and 11 weeks after inoculation of Citrobacter andNitrosomonas bacterial component were used as single inoculant, respectively. The increasingof shoot biomass accumulation was three times as caused by single inoculants (Bacillus sp),and the highest one up to four times of biomass weight caused by a mixture inoculants asconsortium of Azotobacter, Bacillus, and Nitrosomonas spp. That selective inoculant hasopportunity to be used for jatropha farming, and this basic study is meaningful to jatropacultivation for standing to bio-fuel resources.Keywords: Jatropha curcas L., inoculants, Azotobacter, Bacillus, Chromobacterium,Citrobacter, Nitrosomonas, Rhizobium , Spaerotillus natans.Kata kunci: Jatropha curcas L., inoculants, Azotobacter, Bacillus, Chromobacterium,Citrobacter, Nitrosomonas, Rhizobium , Spaerotillus natans.
PRODUCTION OF ACID PHOSPHATASE IN BACILLUS SP. ISOLATED FROM FOREST SOIL OF GUNUNG SALAK NATIONAL PARK Rahmansyah, Maman; Sudiana, I Made
JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i3.3140

Abstract

ABSTRAKProduktivitas Fosfatase Asam pada Bacillus sp. yang Diisolasi dari Tanah Hutan TamanNasional Gunung Salak. Pada pengamatan ini dilakukan karakterisrik bakteri pelarut fosfatyang diisolasi dari tanah hutan Taman Nasional Gunung Salak. Sebanyak 21 koloni hasil isolasidiuji terhadap produktivitas enzim fosfatase berdasar pelarutan media mengandung fosfat.Isolat yang terkuat melarutkan fosfat diidentifikasi sebagai Bacillus sp. Pada pengamatanlanjutan terhadap strain teruji dilakukan penumbuhan pada media cair selama 90 jam inkubasi,dan hasilnya ternyata mampu melarutkan fosfat inorganik (Pi) dari sumber trikalsium fosfat(Ca-Pi) dan alumunium fosfat (Al-Pi) masing-masing pada kisaran 1,2 sampai 152 dan 0.8 sampai25 mg.L-1; dan menunjukkan aktifitas enzim fosfomonoesterase antara 0.2 sampai 1.01 unitpada media yang mengandung larutan para-nitrophenylphosphate sebagai media fosfatorganik (Po) artifisial. Konsumsi glukosa pada media yang diukur selama pertumbuhan sejalanpula dengan produk ortofosfat sebagai akibat adanya aktifitas enzim fosfatase. Peningkatanfosfatase juga sejalan dengan bertambahnya biomassa sel bakteri dan penambahan produkasam glukonat. Penurunan pH dari 7 menjadi 5 diakibatkan peningkatan produk asam glukonatdi dalam media tumbuh. Bakteri pelarut fosfat yang berasal dari tanah hutan Taman NasionalGunung Salak dapat memproduksi fosfatase asam untuk memineralisasi sumber-sumber fosfatmenjadi sumber nutrisi yang siap digunakan oleh akar tumbuhan, dan itu merupakan prediksikuat untuk menjadikan isolat bakteri pelarut fosfat sebagai sumber bahan pupuk hayati.Kata kunci: Bacillus sp., tanah hutan, fosfatase asam, Ca-Pi, Al-Pi.
KESIAGAAN PAKAN PADA TERNAK SAPI SKALA KECIL SEBAGAI STRATEGI ADAPTASI TERHADAP PERUBAHAN IKLIM MELALUI PEMANFAATAN BIODIVERSITAS FLORA LOKAL Maman Rahmansyah; Arwan Sugiharto; Atit Kanti; I Made Sudiana
Buletin Peternakan Vol 37, No 2 (2013): BULETIN PETERNAKAN VOL. 37 (2) JUNI 2013
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v37i2.2427

Abstract

The presence of local floral resources as forage is essential, especially for the farm survival in anticipaty the impact of the global climate change. The availability of forage in the dry land ecosystems depends on water supply. However, the climate change has caused the change on rainfall pattern including the initiation of the dry season. The problem should be anticipated by the local farmers’ readiness in providing sufficient feed and their ability in performing the excellent management during all seasons. In some areas of East Bali and Nusa Tenggara (West andEast), dry climate crisis 2004 led to feed scarcity. Therefore, this study was conducted in order to identify the potential forage resources in the arid land. The discussion is focused on the results of survey and secondary data collection. Based on the results, there were 22 plants that could be used as forage which were abundant during rainy season, whereas 15 others were able to be forage and silage during the transitional season and along the dry season. The results also showed that the introduction of silage fermentation technique was required as the way to preserve feed for the supply during the dry season. In this study the role of polymer compounds hydrolyzing microbes and the fermentation process by the GRAS (Generally Recognized as Safe) microbes preceeded the role of Lactobacillus plantarum were observed. The results of this study may become a reference in anticipating the global climate change impacts in dry land areas by the application of functional microbial technology in small local farms’ feed management activities.(Key words: Local floral resources, Dry land climate, Fermented feed, Lactobacillus plantarum)
PERBANDINGAN POLA ROMBAK SELULOSA OLEH BEBERAPA JAMUR BASIDIOMYCETES MAMAN RAHMANSYAH
BERITA BIOLOGI Vol 3, No 9 (1989)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v3i9.1295

Abstract

MAMAN RAHMANSYAH, 1989,Comparative capability of cellulose degradation by Basidiomy cetes fungi.Berita Biologi 3(9) 450 - 453.Cellulosa is natural resource that still found abandon and its can be composted by themselves naturaly to the earth.Basidiomycetes fungi offered to convert those waste organic matter into useful product.Four Basidiomycetes fungi (Auricularia polytricha, Coprinus cinereus, Pleurotus qstreatus and Volvariella valcacea) have been tested to degrade riee straw, Whatman filter paper and cellulose powder. Three replicates of 3 grams of media were inoculated by different fungus land were fermented for 21 days at room temperature (± 28 C). Biodegradation rare was measured by the lost weight basis of media and bioconvertion rate was detected by stating ability of enzime (cellulose), which were extracted from cellulose powder culture,to convert CMC substrat and glucose production.The result shows that C. cinereus has almost 3 to 4 times degrading and converting capability compared to other fungi.
PENGARUH PERLAKUAN AWAL TERHADAP BENIH TOMAT (LYCOPERSICON ESCULENTUM Mill.) MAMAN RAHMANSYAH
BERITA BIOLOGI Vol 3, No 9 (1989)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v3i9.1301

Abstract

MAMAN RAHMANSYAH,1978.Effect of preconditioning treatments on tomato seeds.Berita Biologi 3(9): 473-4 75. The study the effect of preconditioning treatments for tomato seeds on plant growth and production,five thousand tomato seeds were slowly rehydrated from 6 to 12% moisture content.The treated seeds were sown on moist straw paper at low (26°-28°C)and high (26°- 36°C fluctuated temperatures,others were planted after storage for 48 and 60 hours in the in-cubator (40 C).The germination percentages of seeds,pollen fertility, the percentage of fruits developed, the average weight of fruit and the weight of 1000 seeds were evaluated.Plants derived from rehydrating seeds and stored for 48 hours at 40°C gave the best performance on their germination (more than 90%),pollen fertility (95%) and also the highest total fruits harvested but not on fruit and seed weights.The best result of seed treatment were 12% of moisture content and its were stored for 48 hours at 40°C
CELLULOLYTIC BACTERIA OF SOIL OF GUNUNG HALIMUN NATIONAL PARK I Made Sudiana; Ritai Dwi Rahayu; Hartati Imanuddin; Maman Rahmansyah
BERITA BIOLOGI Vol 5, No 6 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i6.1077

Abstract

The population of aerobic cellulolyitic bacteria (ACB) of soil Gunung Halimun National park and its celulolytic capacity were studied.The soil samples were collected from various altitude (500-1500) m asl.Microbial isolation was performed by culture enrichment technique with CMC (carboxymethyl cellulose) as the major carbon sources.The quantitative determination of ACB was performed by growing the microbes on CMC containing media, and utilizing congored as an indicator.ACB was indicated by formation of clearing zone surrounding growing colony.Cellulytic capacity of each isolates was determined by analysing the ratio of colony and clear zone formation.ACB were quite heterogenous include Bacillus sp., Clostridium sp., Chromobacterlum sp., Enterobacter sp., Moraxella sp. and Pseudomonas sp.
Co-Authors Antonius, S Arwan Sugiharto Atit Kanti Dwi Agustiyani Hartati Imamuddin Hartati Imanuddin I Made Sudiana I Made Sudiana Imanuddin, Hartati LATUPAPUA, H.J.D. Latupapua, H.J.D. Martono, Budhi Muslichah, Dwi Agustiyani NUNIK SULISTINAH Nur Laili Rahayu, Ritai Dwi Ritai Dwi Rahayu Sarjiya Antonius Sri Widawati Suliasih Suliasih Sulistiani ., Sulistiani Yati Sudaryati Soeka