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Rarah Ratih Adjie Maheswari
Bagian Teknologi Hasil Ternak, Departemen Ilmu Produksi dan Teknologi Peternakan, Fakultas Peternakan, Bogor Agricultural University (IPB)
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Identifikasi Laktoferin Pada Susu Kambing Kacang Dengan Metode Imunodifusi Radial Tunggal Dan Natrium Dodesil Sulfat Poliakrilamida Elektroforesis Gel Rarah Ratih Adjie Maheswari; Joni Setiawan; Slamet Mulyanto; Imas Batubara; Cece Sumantri; Akhmad Farajallah
Jurnal Ilmu Pertanian Indonesia Vol. 12 No. 3 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (314.481 KB)

Abstract

Kacang goat is one of Indonesian local goat which has not been optimized in exploration. Kacang goat has potency as a dairy goat. Milk and colostrum from this type of goat is one of lactoferrin sources which has various benefit, such as antimicrobial activity. The milk as a lactoferrin source is expected to be a solution for bacterial 9astrointestinal infection cases which is a major problem in Indonesia. This research described the identification of lactoferrin from milk and colostrum of kacang goat by single radial immunodiffusion (SRID) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). SRID is based on the diffusion of whey protein from a circular well into a homogeneous gel containing anti-lactoferrin. SDS-PAGE was performed in 7.5%  polyacrylamide gel. Both methods were able to identify lactoferrin in colostrum and milk from the sample, but SRID showed low sensitivity toward low concentration of lactoferrin in both colostrums and milk. The estimation of lactoferrin molecular weight by relative mobility of protein from the bands that perform of colostrum and milk of kacang goat is approximately 74,100 Dalton. Based on the ring diameter of the precipitin, the lactoferin level in colostrum and milk increased until 48 hours after postpartum and subsequently decreased. Keywords: colostrum, milk, kacanggoat, lactoferrin, SRID, SDS-PAGE
Stability of Two Probiotics Bacteria of Goat Milk Yoghurt in Rat Digestive Tract RARAH RATIH ADJIE MAHESWARI; I KOMANG GEDE WIRYAWAN; GINA LESMANA MADUNINGSIH
Microbiology Indonesia Vol. 2 No. 3 (2008): December 2008
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.271 KB) | DOI: 10.5454/mi.2.3.6

Abstract

Increased age will affect the bacterial population of the human digestive tract, in which many bacteria will adapt and colonize different sites. Combining probiotics and prebiotics in what has been called a synbiotic could beneficially affect the host by improving survival and implantation/adhesion of live microbial dietary supplements in the gastrointestinal flora. Probiotic bacteria are expected to survive in the digestive tract to give health effects to the host by balancing the intestinal microflora. The addition of fructo-oligosaccharides is expected to increase viability and growth of probiotics in the digestive tract. The objective of the current research is to investigate the effect of probiotic yoghurt and synbiotic yoghurt of Etawa Breed Cross Saanen (PESA) goats together with fructo-oligosaccharides, on female rats and to study the stability of probiotic bacteria in the digestive tracts. The results showed that synbiotic yoghurt intake had no significant influence (p>0.05) on ration consumption, body weight gain, feed conversion, final body weight and mortality. The synbiotic intake significantly increased the population of Bifidobacterium longum (p
Co-Authors ACHMAD FARAJALLAH Cece Sumantri GINA LESMANA MADUNINGSIH I Komang Gede Wiryawan Imas Batubara Joni Setiawan Purwantiningsih Sugita Slamet Mulyanto Tiltje Andretha Ransaleleh Wasmen Manalu