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All Journal Jurnal Veteriner Jurnal Ilmu Ternak Veteriner JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Partner Animal Production : Indonesian Journal of Animal Production Jurnal Produksi Ternak Terapan (JPTT) Jurnal Kedokteran Hewan Farmers: Journal of Community Services Jurnal Agripet Animal Production
Siti Darodjah Rasad
Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary Other

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Journal : Jurnal Agripet

 1 
Tingkat Kematangan Inti Oosit Sapi Setelah 24 Jam Presevasi Ovarium Rini Widyastuti; Siti Darodjah Rasad
Jurnal Agripet Vol 15, No 2 (2015): Volume 15, No. 2, Oktober 2015
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v15i2.2417

Abstract

(Nuclear maturity of bovine oocyte after 24 hours ovary preservation)ABSTRACT. The objective of the research was to investigate their meiotic competence or nuclear maturity of bovine oocytes maturity in vitro after 24 hour preservation on 5C. Oocytes were collected by slicing the ovaries in modified phosphate buffer saline (m-PBS). Selected cumulus-oocyte complexes (COCs) homogenous ooplasm were cultured in maturity medium at 38C in humidified atmosphere of 5% CO2 incubator. After 24 hours, oocytes stained for nuclear maturitys evaluation. The proportion of oocytes at metaphase II (MII) was significantly difference (P 0.05) on oocytes that 24 hours preservated (44.21 3.04%) vs oocytes from fresh ovary (73.97 9.32%) (P0.05). These results indicated that 24 hours preservation bovines ovary on 50C cause decreases of nuclear oocyte maturity.
Analisa Mikrosatelit dalam Bioteknologi Reproduksi Ternak (Suatu Kajian Pustaka) Siti Darodjah Rasad
Jurnal Agripet Vol 9, No 2 (2009): Volume 9, No. 2, Oktober 2009
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v9i2.629

Abstract

Microsatellite analysis in biotechnology of animal reproduction (A Review)ABSTRACT. In year 1970 was found nucleotide sequence which have repeated sequence of nucleotide. with high polymorh and using PCR could be amplified. That sequenz of nucleotide called Microsatelite. Microsatelite consist of 1 6 repeated nucleotide, which is CA repeated as mostly a repeated DNA in the animal (Tautz and Renz, 1984). Based on difference of long and amount of repeated nucleotide, there are three kind of DNA satelite, midi-, mini- and microsatelite (Matiat and Vergmaud, 1982). Microsatelite analysis was used to analyze of paternity and identity of animal, which was done as a conventional analysis with blood group analysis. The advantage of microsatelite analysis compare to blood group system are the exclution probability was high (EXP 99.9%), needs small sampel (tissues, sperm or follicel of hair), could be use for all animal without special age and possible for died animal.
Evaluasi Penampilan Reproduksi Sapi Perah (Studi Kasus Di Perusahaan Peternakan Sapi Perah KUD Sinarjaya) Siti Darodjah Rasad
Jurnal Agripet Vol 9, No 1 (2009): Volume 9, No. 1, April 2009
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v9i1.621

Abstract

Evaluation performance reproduction on dairy cattle (Case study in sinarjaya dairy cattle cooperationABSTRACT. Aim of the identification of performance reproduction was to find out the successfully of reproduction management in the dairy cattle firm or farmers. Performance reproduction of dairy cattle include days open, calving interval, service per conception, first service postpartum and length of the pregnancy. All of that performance reproduction values was ideal value and close relation to the production and that values was difference between farmer and depend of management themselves. Sinar Jaya cooperation is one of dairy cattle cooperation, since 1977 with the population about 1554 dairy cattles in year 2006, has been opportunity to find out the performance reproduction of dairy cattle in that cooperation. Dairy cattle about 197 cattles was used as a sample of the treatment, which were minimum two times calving period with the completely recording of reproduction activity. The method of this research was descriptive method and the data was collected as a census method. As a conclusion, reproduction performance for the first service postpartum, days open and calving interval were ideal values but service per conception was bigger than optimal value.
Effect of Skim Nanoparticles on the Motility and Kinematics of Simmental Cattle Frozen Semen Anggraeni, Fitri Dian; Rasad, Siti Darodjah; Solihati, Nurcholidah
Jurnal Agripet Vol 24, No 2 (2024): Volume 24, No. 2, October 2024
Publisher : Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v24i2.31736

Abstract

Nanotechnology has positively impacted the development of extenders used in the cryopreservation of spermatozoa in livestock. The inclusion of nano skim in the extender is expected to preserve semen quality after thawing. This study aimed to determine the optimal concentration of nano skim. Various concentrations of nano skim (0%, 1.66%, 3.33%, 5%, 6.66%, 8.33%, and 10%) with the addition of 10% nano egg yolk in each extender were compared. Fresh semen samples were collected from three three-year-old Simmental bulls using an artificial vagina. The study parameters included motility (total motility, progressive motility) and kinematics (velocity curve linear (VCL), velocity straight linear (VSL), velocity average pathway (VAP), amplitude lateral head (ALH), beat cross frequency (BCF), straightness (STR), linearity (LIN), and wobble (WOB). A completely randomized design was employed, and data were analyzed using analysis of variance (ANOVA). Fresh semen dilution results indicated that only the control group (0%) and the nano skim group with concentrations of 6.66% and 8.33% were eligible for further processing into frozen semen. The results showed no significant difference between the three treatment groups on sperm motility and kinematics of Simmental cattle after thawing (p0.05). The control group exhibited the highest percentage of total motility, VCL, VAP, and ALH values, while the 6.66% nano skim group outperformed the 8.33% nano skim group. The highest percentage of progressive motility and VSL value was observed in the 6.66% nano-skim group compared to the 8.33% nano-skim and control groups. The highest values of BCF, WOB, LIN, and STR was observed in the nano-skim group at 8.33% compared to the nano-skim group at 6.66% and control. The study concluded that 6.66% nano skim was the optimal concentration to maintain the quality of frozen semen of Simmental cattle.
Co-Authors . Paggi Anggraeni, Fitri Dian Annisa Yusrina Annisaa Yusrina Bambang Hadisutanto Bambang Purwantara Fahmy Avicenna HARSA, DZAKY FATIH Ilham Nugraha Iwan Setiawan K Winangun Kikin Winangun N Solihati Nena Hilmia Nurcholidah Solihati Nurcholidah Solihati Nurcholidah Solihati Nurcholidah Solihati Rangga Setiawan Rangga Setiawan Rini Widyastuti Sari, Aprilianna P.Z.N.L. Soeparna Soeparna Soeparna Soeparna, Soeparna Sutarman Muhardja Toha, Toha Winangun, Kikin