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Journal : Animal Production

Estrous Performance of Etawah Crossbred Goats Following Different Estrous Synchronization Methods Solihati, Nurcholidah; Rasad, Siti Darodjah; Winangun, Kikin; Toha, Toha
ANIMAL PRODUCTION Vol. 23 No. 1 (2021)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2021.23.1.74

Abstract

The objective of the research was to determine the estrous profile of Etawah Crossbred goats after estrous synchronization with different methods. Eighteen does aged 12-24 month old were divided in three groups to receive estrous synchronization treatments (T1 = 14 days intravaginal implant of 60 mg of progesterone (MPA), T2 = two times injection of 5 mg PGF2α (lutalyse) in 11 days interval, and T3 = 10 days of intravaginal implant of 60 mg of progesterone (MPA) + injection of 5 mg PGF2α 48 hours before removal) with six replications. The parameters consisted of estrous behaviour, changes in size and colour of vulva, and duration of estrus when the number of superficial and keratin cells were dominating in the vaginal mucus cell. Data from estrous behaviour and score of vulvar colour was analyzed using Kurkal Wallis test, while onset of estrus, size of vulva slit and estrous duration was analyzed using ANOVA and Duncan test. The result showed that estrous behaviour and changes in color and size of vulva were not significantly different, but estrous duration was significantly different. Estrous duration in T1 (31.30 hour) and T2 (31.10 hour) was significantly longer than that of T3 (11.36 hour). It is concluded that different methods of estrus synchronization affected estrous quality equally but it affected the estrous duration differently based on vaginal mucus cells. Treatment implant vaginal sponge content progesterone for 14 days and double injection of PGF2α with 11-day interval given longest estrous duration.
X-Y Chromosom Bearing Sperm Proportion of Local Ram after Sexing with Different Combination of Bovine Serum Albumin (BSA) Concentration Solihati, Nurcholidah; Rasad, Siti Darodjah; Hilmia, Nena; Winangun, Kikin; Toha, Toha
ANIMAL PRODUCTION Vol. 25 No. 1 (2023)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2023.25.1.137

Abstract

The research aims to evaluate proportion of X-Y chromosom bearing sperm after sexing of local ram semen with different combination of BSA concentration. The research object was ten ejaculated semen of local ram, three years old. The research design used CRD (completely randomize design) with four treatments of BSA concentration combination on upper and bottom layer (T1: 3% & 6%, T2: 4% & 6%, T3: 5% & 10%, and T4: 6% & 12%), and 10 repetitions. Data analysis using analysis variance and Duncan’s Multiple Range Test. The result showed that combination of BSA concentration was significantly effect on proportion of X-Y chromosome bearing sperm of local ram sperm. The higher average proportion of X- and Y- chromosome bearing sperm was obtained at combination 5% (75.55±1.09% for X) at upper layer and 10% BSA (76.45±1.12% for Y) of bottom layer. The conclusion is that combination of BSA concentration significantly effect on proportion of X-Y chromosome bearing sperm, and concentration of 5% and 10% BSA at upper layer and bottom layer gave the higher proportion of X-Y chromosome bearing sperm of local ram sperm.