Article Per Year (5 Year)
p-Index From 2020 - 2025
1.633
P-Index
This Author published in this journals
All Journal Jurnal Veteriner Jurnal Ilmu Ternak Veteriner JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Partner Animal Production : Indonesian Journal of Animal Production Jurnal Produksi Ternak Terapan (JPTT) Jurnal Kedokteran Hewan Jurnal Agripet Animal Production
Siti Darodjah Rasad
Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary

Published : 15 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Jurnal Ilmu Ternak Veteriner

 1 
Effect of Incubation Time During Sperm Sexing Process on Sperm Quality of Pasundan Bull Siti Darodjah Rasad; Nurcholidah Solihati; Kikin Winangun; Annisa Yusrina; Fahmy Avicenna
Jurnal Ilmu Ternak dan Veteriner Vol 25, No 3 (2020): SEPTEMBER 2020
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v25i3.2494

Abstract

The research was conducted to evaluate the effect of incubation time on viability, plasma membrane integrity, abnormality, and DNA integrity of sexed Pasundan’s bulls sperm. The sperm sexing used 5% and 10% concentrations of Bovine Serum Albumin (BSA).  A completely randomized design with three treatments and six replications was used in this study. The data were analyzed using variance analysis followed by Duncan’s multiple distance test.  Parameter evaluated were sperm longevity, plasma membrane integrity (PMI), abnormality, and DNA integrity of sexed Pasundan bulls sperm. Results showed that incubation time gave significant effect (P<0.05) on the longevity of sperm, but not on the PMI of Pasundan bulls sexed sperm.  The incubation time of 45 minutes gave the highest value of longevity sperm on the upper layer (4.33 days) and the lower layer (4.17 days). Furthermore, the abnormality of sperm X in the upper layer was 4.00%-4.20% and the lower layer was 4.10%- 4.40%.  Meanwhile, the DNA integrity of an upper layer was 98.16%-98.66%, and the lower layer was 97.83%-98.58%.  It is concluded that 45 minutes of incubation time significantly affected the longevity of sperm, but not plasma membrane integrity, abnormality, and DNA integrity of Pasundan bulls sexed sperm.
Effectiveness of Various Glycerol Concentrations as a Cryoprotectant in Frozen Semen of Pasundan Cattle Siti Darodjah Rasad; N Solihati; K Winangun
Jurnal Ilmu Ternak dan Veteriner Vol 27, No 3 (2022): September 2022
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v27i3.3066

Abstract

This study was conducted to determine the effectiveness of Glycerol as a cryoprotectant with various concentrations on the quality of Pasundan cattle semen.  Semen was collected from seven bulls of Pasundan Cow using an artificial vagina twice a week for three months.  The semen sample was added with a TRIS-Egg Yolk Extender containing 20% (v/v) egg yolk and treated with the addition of Glycerol with five different concentrations (G5=5%, G6=6%, G7=7%, G8= 8%, and G9=9%) were then performed with cryopreservation. A Completely Randomized Design (CRD) was used to examine the effect of five different concentrations of Glycerol on motility, intact plasma membrane (IPM), the integrity of acrosome cap (IAC), abnormalities, and recovery rate (RR) of spermatozoa after cryopreservation (post-thawing).  The results of diluted Pasundan cattle semen evaluation showed that the addition of 7% Glycerol (G7) resulted in the best motility and IAC values (83.68% and 72.84%), the addition of 7% and 8% Glycerol (G7 and G8) resulted in the best IPM values (85.00% and 84.50%).  The addition of 6%, 7%, 8%, and 9% Glycerol (G6, G7, G8, and G9) resulted in the lowest abnormality values (1%).  On the post-thawing Pasundan cattle semen evaluation, the addition of 7% Glycerol (G7) resulted in the best motility, IAC, IPM, and RR values (54.49%, 38.57%, 54.29%, and 72.28%).  Meanwhile, adding various Glycerol concentrations did not significantly affect the abnormality value of post-thawing spermatozoa.  Generally, the addition of 7% Glycerol in semen extenders shows optimal results as a cryoprotectant.
Co-Authors . Paggi Anggraeni, Fitri Dian Annisa Yusrina Annisaa Yusrina Bambang Hadisutanto Bambang Purwantara Fahmy Avicenna HARSA, DZAKY FATIH Ilham Nugraha Iwan Setiawan K Winangun Kikin Winangun N Solihati Nena Hilmia Nurcholidah Solihati Nurcholidah Solihati Nurcholidah Solihati Nurcholidah Solihati Rangga Setiawan Rangga Setiawan Rini Widyastuti Soeparna Soeparna Soeparna Soeparna, Soeparna Sutarman Muhardja Toha, Toha Winangun, Kikin