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Effectiveness of Leaves Extract Fractions of Archidendron jiringa (Jack) I.C Nielsen Against Microbes Oom Komala; Sri Wardatun; Lia Puspita Sari
Molekul Vol 14, No 2 (2019)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (296.568 KB) | DOI: 10.20884/1.jm.2019.14.2.538

Abstract

Archidendron jiringa(Jack) I.C Nielsen) contains a lot of essential oils, saponins, alkaloids, terpenoids, steroids, tannins, glycosides, and flavonoids. Flavonoid is a secondary metabolite compound in a plant as antimicrobial. This study aims to determine the antimicrobial activity of fractions of ethanol 96%, n-hexan, ethyl acetate, and ethanol-water leaves extractand determine levels of favonoids.  A. jiringaleaves extract were tested against Streptococus mutans, Pseudomonas aeruginosa, and Candida albicans.  The research using agar disc diffusion technique for antimicrobial  activity and colorimetric method to know the total of flavonoid. Chloramphenicol and ketoconazole were used as a reference standard. The result of this study shows fractions of ethanol 96%,  n-hexane,  ethyl acetate, and  ethanol-water A. jiringaleaves extract  can inhibit microbial growth of S. mutans, P. aeruginosa, and C. albicans.Total of flavonoids on the extract respectively are 1.13%, 0.494%, 2.337%, and 0.549%. determined with complementary colorimetry to each leaf extract fractionA. jiringawith aluminum chloride method. Absorption spectrum measurement using a spectrophotometer.  The higher  percent of flavonoids would cause greater    bacteriainhibitory  zone but  only  0.090 for  pearson correlation value. Conclusions ethyl acetate leaves extract most effective inhibit microbial growth from the other fractions.
Perbedaan Pakan Komersil Dengan Protein Rendah dan Pakan Alami Terhadap Pertumbuhan Benih Ikan Baung (Hemibagrus nemurus) Nugraha, Sujaka; Anjani, Tiara Puspa; Lia Puspita Sari; Humairani, Humairani; Rahma Mulyani; Indah Anggraini Yusanti
Jurnal Ilmu-ilmu Perikanan dan Budidaya Perairan Vol. 19 No. 1 (2024): Jurnal Ilmu-ilmu Perikanan dan Budidaya Perairan
Publisher : University of PGRI Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31851/jipbp.v19i1.13852

Abstract

Pemilihan jenis pakan adalah salah satu faktor yang mempengaruhi tingkat keberhasilan budidaya ikan. Penggunaan pakan komersil dengan protein rendah 15 % kurang optimal dalam pertumbuhan benih ikan baung, selain itu biaya produksi yang diperlukan juga cukup tinggi. Penambahan pakan alami seperti cacing sutra adalah salah satu jenis pakan alternatif yang dapat digunakan dan diketahui mempunyai kandungan gizi protein tinggi yang disukai oleh benih ikan baung. Kombinasi antara pakan komersil dan cacing sutra pada penelitian ini diharapkan dapat meningkatkan keberlangsungan hidup dan mempercepat pertumbuhan benih ikan baung. Penelitian ini dilakukan selama 16 hari menggunakan benih ikan baung dengan ukuran berkisaran antara 1 - 1.15 cm dengan menggunakan metode rancangan acak lengkap dengan 3 perlakuan yaitu pemberian pakan pelet komersil (P), pakan cacing sutera (C), serta pakan kombinasi antara pelet komersil 50 % dan cacing sutera 50 % (CP) yang diulang sebanyak 3 kali ulangan. Hasil penelitian menunjukan bahwa perlakuan jenis pakan yang berbeda berpengaruh nyata (P<0.05) terhadap panjang dan bobot akhir, pertumbuhan panjang mutlak, laju pertumbuhan spesifik, serta tingkat kelangsungan hidup benih ikan baung, namun tidak berpengaruh nyata (P>0.05) terhadap koefisien keragaman dan faktor kondisi benih ikan baung. Perlakuan pemberian pakan kombinasi pelet komersil dan cacing sutera (50%:50%) pada benih ikan baung dapat meningkatkan persentase kelangsungan hidup sebesar 89 % selama 16 hari pemeliharaan.   The choice of feed type is one of the factors that influences the success rate of fish farming. The use of commercial feed with a low protein of 15% is less than optimal for the growth of baung fish fry, besides that the production costs required are also quite high. The addition of natural feed such as silk worms is one type of alternative feed that can be used and is known to have high protein nutritional content which is preferred by baung fish fry. The combination of commercial feed and silk worms in this research is expected to increase survival and accelerate the growth of baung fish fry. This research was carried out for 16 days using baung fish fry with sizes ranging from 1 - 1.15 cm using a completely randomized design method with 3 treatments, namely commercial pellet feeding (P), silk worm feeding (C), and a combination of 50 commercial pellets. % and 50% silk worms (CP) which were repeated 3 times. The results of the study showed that treatment with different types of feed had a significant effect (P<0.05) on final length and weight, absolute length growth, specific growth rate, and survival rate of baung fish fry, but had no significant effect (P>0.05) on the diversity coefficient. and factors regarding the condition of baung fish seeds. The treatment of feeding a combination of commercial pellets and silk worms (50%:50%) to baung fish fry can increase the survival percentage by 89% for 16 days of rearing.