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Aplikasi metabolit sekunder dari tiga isolat Pseudomonas fluorescens untuk mengendalikan penyakit antraknosa pada daun kakao Fitrianti FITRIANTI; Loekas - SOESANTO; Endang MUGIASTUTI; Murti Wisnu Ragil SASTYAWAN; Abdul MANAN
E-Journal Menara Perkebunan Vol 90, No 1 (2022): April, 2022
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v90i1.483

Anthracnose caused by Colletotrichum gloeosporoides is an important disease in cocoa. This research aimed to determine the effectiveness of secondary metabolites derived from three isolates of Pseudomonas fluorescens to control cocoa leaves anthracnose, and their influence on the growth of cocoa plants. The research was conducted at a smallholder cocoa plantation in Putat Village, Patuk District, Gunung Kidul Regency, Yogyakarta for four months. A randomized block design was used with four replicates and seven treatments consisted of control, application of secondary metabolites from P. fluorescens P60, P. fluorescens P20, P. fluorescens P8, combination P. fluorescens P60 + P20, P. fluorescens P60 + P8, and P. fluorescens P20 + P8. Variables observed were disease intensity, infection rate, number of healthy shoots and qualitative phenolic compound. Resultsof the research showed that the secondary metabolites of P. fluorescens P60, P20 and P8 alone or in combination suppressed the disease intensity by42.01-54.50%. The infection rate caused by metabolite secondary of P. fluorescens P60, P20, P8, P. fluorescens P60+P20, P. fluorescens P60+P8,and P. fluorescens P20+P8 was 0.23; 0.25; 0.26; 0.26; 0.31; and 0.24 units/day, respectively. The secondary metabolites of P. fluorescens P60 increased the number of healthy shoots by 67.44 %. The secondary metabolites of P. fluorescens P60 increased phenolic compounds (tannin, saponin, and glycosides) in cocoa leaves.[Keyword: leaves anthracnose, Colletotrichum gloeosporoides, cocoa, secondary metabolites, Pseudomonas fluorescens] AbstrakAntraknosa yang disebabkan oleh Colletotrichum gloeosporioides merupakan penyakit penting pada tanaman kakao. Penelitian bertujuan mengkaji keefektifan metabolit sekunder dari tiga isolat Pseudomonas fluorescens untuk mengendalikan penyakit antraknosa pada daun kakao, serta pengaruhnya terhadap pertumbuhan tanaman kakao. Penelitian dilaksanakan di perkebunan kakao rakyat, Desa Putat, Kecamatan Patuk, Kabupaten Gunung Kidul, Yogyakarta selama empat bulan. Penelitian menggunakan rancangan acak kelompok untuk menguji 7 perlakuan yang diulang 4 kali, perlakuan yang diuji adalah kontrol, aplikasi metabolit sekunder P. fluorescens P60, P. fluorescens P20, P. fluorescens P8, kombinasi P. fluorescens P60+P20, P. fluorescens P60+P8, dan P. fluorescens P20+P8. Variabel pengamatan meliputi intensitas penyakit, laju infeksi, jumlah tunas sehat, dan kandungan senyawa fenol secara kualitatif. Hasil penelitian menunjukkan bahwa perlakuan metabolit sekunder P. fluorescens P60, P20, dan P8 secara tunggal dan gabungan mampu menekan intensitas penyakit berkisar 42,01-54,50%. Laju infeksi perlakuan metabolit sekunder P. fluorescens P60, P. fluorescens P20, P. fluorescens P8, kombinasi P. fluorescens P60+P20, P. fluorescens P60+P8 dan P. fluorescens P20+P8 berturut-turut 0,23; 0,25; 0,26; 0,26; 0,31; dan 0,24 unit/hari. Metabolit sekunder P. fluorescens P60 meningkatkan jumlah tunas sehat sebesar 67,44%. Perlakuan P. fluorescens P60 meningkatkan senyawa fenol (saponin, tanin, dan glikosida) pada daun kakao.[Kata kunci: antraknosa daun, Colletotrichum gloeosporoides, kakao, metabolit sekunder, Pseudomonas fluorescens]

Evaluation of Effervescent Tablet Formulation of Trichoderma harzianum Raw Secondary Metabolites Toward Fusarium Wilt on Pepper Loekas Soesanto; Dede Herdiyana Ikbal; Endang Mugiastuti; Murti Wisnu Ragil Sastyawan; Tamad Tamad
AGRIVITA, Journal of Agricultural Science Vol 44, No 2 (2022)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v44i2.3699

Fusarium oxysporum f.sp capsici, which causes chili-fusarium wilt disease, may be regulated by a secondary metabolite of Trichoderma harzainum. Effervescent tablets are used because liquid formulations have some drawbacks. The purpose of this study is to qualitatively determine T. harzianum’s best crude secondary metabolites, growth and yield, and phenolic compound content in chili crops in foamed tablet formulations against F. oxysporum in vitro. The in vitro study used 6 replicates, a completely randomized design, and 4 treatments consisted of controls and 4, 6, and 8 tablets. Under in vivo conditions, the experiment used a randomized block designs with 4 replicates, eight treatments consisting of controls, fungicides (benomyl), and four, six, or eight tablets per day before or after inoculation. The variables observed were antagonist testing, incubation time, disease intensity, disease incidence, AUDPC, germination rate, plant height, root fresh weight, and qualitative phenolic composition. The results of the study showed that the best dose of T. harzianum’s crude secondary metabolite in vitro was 4 tablets. Medications in in-plant studies delayed the incubation period by 64.11%, suppressed disease outbreaks by 58.34%, reduced disease intensity by 80.45%, increased plant height by 50.4%, and harvested phenols (saponins, tannins, hydroquinone). The content of the compound has been qualitatively increased.

Potency of two Trichoderma harzianum isolates in liquid and solid organic formula for controlling bacterial wilt on tomato in the field Loekas Soesanto; Murti Wisnu Ragil Sastyawan; Nina Nurliana; Endang Mugiastuti
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 22 No. 2 (2022): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.222116-125

This research aimed to determine the potency of two Trichoderma harzianum isolates in solid and liquid organic formulas to control bacterial wilt and on growth and yield of tomato in the field. This research was carried out at the Laboratory of Plant Protection and the tomato field at Banteran Village, Sumbang Sub-District, Banyumas Regency at altitude of 600 m above sea level for four months. A randomized block design was used with five replicates. The treatments were control, T. harzianum T10, T. harzianum T215, combination of T. harzianum T10 + T. harzianum T215, and bactericide (a.i. 20% streptomycin sulfate). Variables observed were pathosystem components (incubation period, infection rate, disease intensity, and late populations of the antagonists), growth components (plant height, plant fresh and dry weight, and root fresh and dry weight), yield components (number of fruits, fruit weight), and phenolic compounds analysis qualitatively. The results showed that T. harzianum T10 + T. harzianum T215 was effective to suppress the disease as 58.61%. The treatment of T. harzianum T10 + T. harzianum T215 was effective to increase crop height, fresh weight of plants, dry weight of plants, fresh weight of roots, dry weight of roots, number of fruits and fruit weight as 38.86, 35.37, 51.67, 24.78, 37.41, 40.61, and 53.22%, respectively. All treatments could increase phenolic compound content qualitatively.

ANALYSIS OF THE INFLUENCE OF HOSPITAL MANAGEMENT INFORMATION SYSTEM WITH STRUCTURAL EQUATION MODELING APPROACH (Case Study of Regional Hospital RAA Soewondo Pati) Murti Wisnu Ragil Sastyawan; Loekas Soesanto; Bambang Purwanggono; Arfan Bakhtiar
Proceeding of The International Conference on Business and Economics Vol. 1 No. 1 (2023): Proceeding of The International Conference on Business and Economics
Publisher : Universitas 17 Agustus 1945 Semarang

The information system is one of the strategic means in patient care-oriented management. So far, Regional Hospital RAA Soewondo Pati has never conducted an evaluation related to the management information system (HMIS), so it is not known that the existing system is effective and efficient to answer patient needs. The purpose of this study was to determine the effect of the hospital management information system with the structural equation modeling method approach. This research is a case study research with data collection method using a questionnaire. The data that has been collected is then analyzed using SEM analysis. The results of his research stated that the quality of the system had a significant positive effect on the use of information systems. There is a significant effect of system quality on user satisfaction. There is a significant positive effect between the quality of information on the intensity of the use of information. In this study, the intensity of the use of information systems does not play a role in increasing user satisfaction in using information systems. User satisfaction has a significant positive effect on net benefits.

Preservation of weeds’ pathogenic fungi in tempeh and tapioca liquid waste and its effectiveness in goatweed (Ageratum conyzoides) Soesanto, Loekas; Mugiastuti, Endang; Ragil Sastyawan, Murti Wisnu; Manan, Abdul
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 23 No. 2 (2023): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.22331-41

This research aimed to determine the best liquid media for the propagation of weed pathogenic fungi, the duration of the fungus storage on the media, and their virulence on goatweed (Ageratum conyzoides). The research consisted of two stages, i.e., the propagation of weed pathogenic fungi in alternative liquid media using a factorial completely randomized design, with the first factor being the pathogenic weed fungus (Curvularia sp., Fusarium sp., and Chaetomium sp.) and the second one being the media (tempeh or tapioca liquid waste) with four replicates. Applications were carried out using a hand sprayer on the underside of weed leaves at a density of 106 conidia or cfu mL-1. Each weed was sprayed with 10 mL of the solution. Variables observed were conidia density, number of colonies, incubation period and symptoms, disease intensity, the area under the disease progress curve (AUDPC), plant height, number of leaves, dry crown weight, and dry root weight. The results showed that tempeh and tapioca liquid waste media could be used as alternative media for multiplying pathogenic fungi. The conidia density of Curvularia sp. was 2.375 × 109 conidia mL-1 higher than that of Fusarium sp. at 1.7 × 109 conidia mL-1 and Chaetomium sp. at 9.5×104 cfu mL-1. Curvularia sp. propagated in tempeh liquid waste was able to cause damage to the leaves of goatweed as shown successively from the incubation period of 3.33 dai or accelerating 81.50%, increasing the disease intensity of 88.78%, and the AUDPC of 713.25% days compared to control. The most effective shelf life of Curvularia sp., Fusarium sp., and Chaetomium sp. in both tempeh and tapioca liquid waste media was found at six weeks at room temperature. Curvularia sp. in tapioca liquid waste could decrease weed height, the number of leaves, shoot dry weight, and root dry weight by 45.11, 28.65, 22.12, and 46.25%, respectively, compared to control.

Aplikasi metabolit sekunder dari tiga isolat Pseudomonas fluorescens untuk mengendalikan penyakit antraknosa pada daun kakao Fitrianti FITRIANTI; Loekas - SOESANTO; Endang MUGIASTUTI; Murti Wisnu Ragil SASTYAWAN; Abdul MANAN
Menara Perkebunan Vol. 90 No. 1 (2022): 90 (1), 2022
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v90i1.483

Anthracnose caused by Colletotrichum gloeosporoides is an important disease in cocoa. This research aimed to determine the effectiveness of secondary metabolites derived from three isolates of Pseudomonas fluorescens to control cocoa leaves anthracnose, and their influence on the growth of cocoa plants. The research was conducted at a smallholder cocoa plantation in Putat Village, Patuk District, Gunung Kidul Regency, Yogyakarta for four months. A randomized block design was used with four replicates and seven treatments consisted of control, application of secondary metabolites from P. fluorescens P60, P. fluorescens P20, P. fluorescens P8, combination P. fluorescens P60 + P20, P. fluorescens P60 + P8, and P. fluorescens P20 + P8. Variables observed were disease intensity, infection rate, number of healthy shoots and qualitative phenolic compound. Resultsof the research showed that the secondary metabolites of P. fluorescens P60, P20 and P8 alone or in combination suppressed the disease intensity by42.01-54.50%. The infection rate caused by metabolite secondary of P. fluorescens P60, P20, P8, P. fluorescens P60+P20, P. fluorescens P60+P8,and P. fluorescens P20+P8 was 0.23; 0.25; 0.26; 0.26; 0.31; and 0.24 units/day, respectively. The secondary metabolites of P. fluorescens P60 increased the number of healthy shoots by 67.44 %. The secondary metabolites of P. fluorescens P60 increased phenolic compounds (tannin, saponin, and glycosides) in cocoa leaves.[Keyword: leaves anthracnose, Colletotrichum gloeosporoides, cocoa, secondary metabolites, Pseudomonas fluorescens] AbstrakAntraknosa yang disebabkan oleh Colletotrichum gloeosporioides merupakan penyakit penting pada tanaman kakao. Penelitian bertujuan mengkaji keefektifan metabolit sekunder dari tiga isolat Pseudomonas fluorescens untuk mengendalikan penyakit antraknosa pada daun kakao, serta pengaruhnya terhadap pertumbuhan tanaman kakao. Penelitian dilaksanakan di perkebunan kakao rakyat, Desa Putat, Kecamatan Patuk, Kabupaten Gunung Kidul, Yogyakarta selama empat bulan. Penelitian menggunakan rancangan acak kelompok untuk menguji 7 perlakuan yang diulang 4 kali, perlakuan yang diuji adalah kontrol, aplikasi metabolit sekunder P. fluorescens P60, P. fluorescens P20, P. fluorescens P8, kombinasi P. fluorescens P60+P20, P. fluorescens P60+P8, dan P. fluorescens P20+P8. Variabel pengamatan meliputi intensitas penyakit, laju infeksi, jumlah tunas sehat, dan kandungan senyawa fenol secara kualitatif. Hasil penelitian menunjukkan bahwa perlakuan metabolit sekunder P. fluorescens P60, P20, dan P8 secara tunggal dan gabungan mampu menekan intensitas penyakit berkisar 42,01-54,50%. Laju infeksi perlakuan metabolit sekunder P. fluorescens P60, P. fluorescens P20, P. fluorescens P8, kombinasi P. fluorescens P60+P20, P. fluorescens P60+P8 dan P. fluorescens P20+P8 berturut-turut 0,23; 0,25; 0,26; 0,26; 0,31; dan 0,24 unit/hari. Metabolit sekunder P. fluorescens P60 meningkatkan jumlah tunas sehat sebesar 67,44%. Perlakuan P. fluorescens P60 meningkatkan senyawa fenol (saponin, tanin, dan glikosida) pada daun kakao.[Kata kunci: antraknosa daun, Colletotrichum gloeosporoides, kakao, metabolit sekunder, Pseudomonas fluorescens]

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