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All Journal Jurnal Fitopatologi Indonesia International Journal of Biosciences and Biotechnology EUGENIA AGRIVITA, Journal of Agricultural Science Jurnal Agro Planta Tropika Jurnal Terapan Abdimas JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal SOLMA Agrin : Jurnal Penelitian Pertanian Biosaintifika: Journal of Biology & Biology Education Journal of Tropical Horticulture Jurnal Agro Wiralodra Jurnal Layanan Masyarakat (Journal of Public Service) Matriks
Loekas Soesanto
Fakultas Pertanian Universitas Jenderal Soedirman Purwokerto
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Environmental Science Medicine & Pharmacology Neuroscience Social Sciences Other

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Application of Bio P60 and Bio T10 in Combination Against Phytophthora Wilt of Papaya Soesanto, Loekas; Kustam, Kustam; Mugiastuti, Endang
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i3.20389

Abstract

Papaya is one of the most widely cultivated horticultural plants. Phytophthora wilt is an important papaya disease which results in production losses. This research aimed to determine the effect of Bio P60 (raw secondary metabolites of Pseudomonas fluorescens P60) and Bio T10 (raw secondary metabolites of Trichoderma harzianum T10) application in combination on Phytophthora wilt and on growth of pepaya. The research was conducted on Californian pepaya farm, Linggasari Village, Kembaran District, Banyumas Regency. Randomized block design was used with six replicates and five treatments consisted of control (Mancozeb 80%), Bio T10 flush + Bio T10 spray, Bio T10 flush + Bio P60 spray, Bio P60 flush + Bio T10 spray, and Bio P60 flush + Bio P60 spray. Variables observed were percentage of healthy leaves, infection rate, number of healthy leaves, and number of healthy pepaya fruit. Result of the research showed that application of Bio P60 and Bio T10 effectively cured Phytophthora wilt with the percentage of healthy leaves as 69.19% compared to control. The combination was able to increase the number of healthy leaves the number of healthy papaya fruit. The novelty of this research is that raw secondary metabolites of biological agents proven to be able to overcome the papaya diseases, which so far cannot be solved. The implication for the development of science is one step ahead in overcoming plant diseases biologically by utilizing raw secondary metabolites. The benefits for the community can overcome papaya plant diseases organically, safely, and environmentally friendly, and inexpensive.
Granular Formulation Test of Pseudomonas fluorescens P60 for Controling Bacterial Wilt (Ralstonia solanacearum) of Tomato In Planta Soesanto, Loekas; Mugiastuti, Endang; Khoeruriza, Khoeruriza
AGRIVITA, Journal of Agricultural Science Vol 41, No 3 (2019)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i3.2318

Abstract

Ralstonia solanacearum is the most devastating bacteria. Pseudomonas fluorescens P60 is a bacterial antagonist. This research aimed to study shelf life, antagonism and the effect of granular application of P. fluorescens P60 to control bacterial wilt and growth of tomato in planta. The research was conducted at the Plant Protection Laboratory and the screen house, Faculty of Agriculture, Jenderal Soedirman University, from October 2018 to March 2019. A randomized block design was used with six treatments and five replicates. The treatments were control, R. solanacearum + 1, 5, 10, and 15 g the granule, and bactericide (Agrimycine sulfate 20%). Variables observed were population density, clear zone, incubation period, disease incidence, disease intensity, area under disease progress curve (AUDPC), crop height, root length, crops fresh weights, and phenolic compound content qualitatively. Result showed that the formulation up to 10 weeks still performed a high P. fluorescens P60 population and good activity. All the granular and the bactericide effectively suppressed the disease indicated by the lenghtening incubation period of 22.77-26.25%, reducing the disease incidence as 60-85%, decreasing disease intensity as 65-85%, and decreasing AUDPC as 75.69-86.11%-days, increasing phenolic compound content qualitatively, and increasing crop height between 24.85-36.17%, and fresh weight between 46.04-57.13%.
PEMANFAATAN BEBERAPA ISOLAT Trichoderma harzianum SEBAGAI AGENSIA PENGENDALI HAYATI PENYAKIT LAYU FUSARIUM PADA BAWANG MERAH IN PLANTA Latifah, Aenul; Kustantinah, .; Soesanto, Loekas
EUGENIA Vol 17, No 2 (2011)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.17.2.2011.4105

Abstract

ABSTRACT The green house research aimed to know the best isolate of T. harzianum in controlling Fusarium wilt , shallot production, and the effect of T. harzianum application time in suppressing the disease. Randomized block design was used with twelve treatments. Each treatment was replicated three times. The treatments were negative control, positive control as wel as extract of ginger isolate, banana isolate, shallot isolate, potato isolate, and ginseng isolates. The treatments were  applied a week before or after inoculation of the pathogen. The result showed that the antagonist extract of ginger isolate applied a week before inoculation decreased the disease intensity by 43.854%. The antagonist extract application applied before or after inoculation were not significant difference . The antagonist of potato isolate extract was the best in increasing number of plants per clump and tuber fresh weight resulting  9.7503 and 13.133 g, respectively. The best extract in increasing root growth was shallot extract. The ginger isolate extract increased root length by 17.672%. Keywords: Trichoderma harzianum isolates, biological control, Fusarium wilt, shallot   ABSTRAK Penelitian rumah kasa ini bertujuan untuk mengetahui isolat T. harzianum yang paling baik dalam mengendalikan penyakit moler dan meningkatkan hasil bawang merah, serta pengaruh waktu pemberian T. harzianum dalam menekan penyakit moler. Rancangan yang digunakan adalah Rancangan Acak Kelompok (RAK), yang terdiri atas 12 perlakuan dengan 3 ulangan. Perlakuan yang dicoba adalah kontrol negatif (tanpa perlakuan T. harzianum dan  Fusarium oxysporum) dan kontrol positif (hanya dengan inokulasi F. oxysporum), serta ekstrak isolat jahe, pisang, bawang merah, kentang, dan ginseng yang diberikan 1 minggu sebelum atau sesudah inokulasi. Hasil penelitian menunjukkan bahwa ekstrak T. harzianum isolat jahe yang disiram sebelum inokulasi Fusarium oxysporum, mampu menurunkan intensitas penyakit sebesar 43,854%. Belum terlihat jelas pengaruh nyata pemberian ekstrak antagonis sebelum maupun sesudah inokulasi patogen. Ekstrak T. harzianum isolat kentang adalah isolat terbaik dalam meningkatkan jumlah anakan per rumpun dan bobot basah umbi, yaitu masing-masing 9,7503 dan 13,133 g, sedangkan ekstrak terbaik dalam pertumbuhan akar yaitu isolat bawang merah yang meningkatkan jumlah akar sebesar 57,452% dan ekstrak isolat jahe menunjukkan pengaruh terbaik meningkatkan panjang akar sebesar 17,672%. Eugenia Volume 17  No. 2  Agustus 2011 Kata Kunci: Isolat Trichoderma harzianum, pengendalian hayati, penyakit layu Fusarium, bawang merah
KEEFEKTIFAN BAKTERI Serratia ENDOSIMBION WBC TERHADAP ULAT GRAYAK (Spodoptera litura F.) DI LABORATORIUM ENTOMOLOGI BBPOPT JATISARI KARAWANG Dwimartina, Fina; Rostaman, Rostaman; Soesanto, Loekas
Jurnal Agro Wiralodra Vol 3 No 1 (2020): Jurnal Agro Wiralodra
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/agrowiralodra.v3i1.39

Abstract

Penelitian ini bertujuan untuk: 1) Mengetahui pengaruh perlakuan bakteri Serratia Endosimbion WBC terhadap mortalitas serangga S. litura; 2) Mengetahui pengaruh pemberian bakteri tersebut terhadap daya konsumsi larva S. litura; 3) Mengetahui pengaruh bakteri tersebut terhadap perkembangan serangga. Penelitian dilaksanakan di Laboratorium Entomologi BBPOPT Jatisari Karawang, pada bulan Maret sampai Juli 2014. Penelitian ini dilakukan dengan metode eksperimen, menggunakan rancangan acak terpisah yang terdiri dari 10 perlakuan dan 2 kali ulangan. Variabel yang diamati adalah mortalitas (%), tingkat konsumsi larva, dan perkembangan larva. Hasil penelitian menunjukkan bakteri Serratia tidak menimbulkan kematian pada serangga uji (mortalitas 0%), tidak berbeda nyata pada daya konsumsi larva, dan tidak berpengaruh nyata terhadap persentasi larva yang menjadi pupa. Bakteri Serratia berpengaruh nyata terhadap berat pupa dan persentasi pupa menjadi imago.
APPLICATION OF RAW SECONDARY METABOLITES FROM TWO ISOLATES OF Trichoderma harzianum AGAINST ANTHRACNOSE ON RED CHILI PEPPER IN THE FIELD Soesanto, Loekas; Mugiastuti, Endang; Suyanto, Agus; Rahayuniati, Ruth Feti
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 20 No. 1 (2020): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j.hptt.12019-27

Abstract

Anthracnose on red chili pepper is one of the highly damaging diseases that difficult to control bypesticides. This study aimed to determine the effect of raw secondary metabolites isolated from two isolates of Trichodermaharzianum towards anthracnose as well as the growth and yield of red chili pepper plants in the field. The research used arandomized block design with five treatments and seven replicates. The treatments tested were control, raw secondarymetabolites of T. harzianum T10 and T213, and their combination. The observed variables were incubation period, diseaseintensity, the late population density of T. harzianum, plant height, root length, fresh and dry weight of the plant and root,flowering time, number of fruits per plant, fresh weight of fruit, and phenolic compound content analysis. The result showedthat the raw secondary metabolites from the combination of the two T. harzianum isolates were the best treatments that couldlengthen the incubation period and decrease the disease intensity as 30.2 and 87.05%, respectively. However, these applicationscould not increase the growth components; while for the yield components, the application could improve the number of fruitsper plant and fresh weight of fruit as 15.33 and 34.53%, respectively.
APPLICATION OF RAW SECONDARY METABOLITES FROM FOUR ENTOMOPATHOGENIC FUNGI AGAINST CHILLI DISEASE CAUSED BY VIRUSES Soesanto, Loekas; Prastyani, Noviana; Sri Utami, Darini; Manan, Abdul
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 20 No. 2 (2020): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j.hptt.220100-107

Abstract

Application of raw secondary metabolites from four entomopathogenic fungi against chilli disease caused by viruses. The purpose of this research was to investigate several kinds of raw secondary metabolits to decrease viral disease in chilli and inspect their side effect to plant growth. This research was conducted at experimental farm, Faculty of Agriculture, Jenderal Soedirman University from November 2018 to March 2019. The chilli seeds used for indicator plant were obtained from virus-symptomatic chilli. The raw secondary metabolites was collected from four microbial isolates used in this study, i.e. Metarhizium anisopliae, Beauveria bassiana (Papua isolate), Lecanicillium lecanii and B. bassiana Bio B10 (Jember isolate). The experiment was arranged in completely randomized design with five replications. Observation was performed on incubation period, disease intensity, AUDPC, germination percentage, plant height, number of leaves, and number of shoots. The result showed that raw secondary metabolites obtained from M. anisopliae gave the best capability to suppress disease development. Application of M. anisopliae raw secondary metabolites reduced incubation period, viral disease intensity as well as AUDPC in 34.22; 77.98 and 79.49%, respectively. The raw secondary metabolites of L. lecanii could increase percentage of germination, plant height, number of leaves, and number of shoots as 100; 38.96; 38.96 and 52.38%, respectively, compared to control.
Raw Secondary Metabolites of Trichoderma harzianum T10 in Tapioca Flour Towards Cucumber Damping-off Soesanto, Loekas; Ilahiyyah, Hidayatul; Mugiastuti, Endang; Manan, Abdul; Rostaman, Rostaman
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.24567

Abstract

Trichoderma harzianum is effective for controlling soil-borne pathogenic fungi and producing secondary metabolites. When applied in the field, the raw secondary metabolites are quickly decreased directly by sunlight. One strategy to avoid degradation is the use of tapioca fluor liquid formula for biological control agents. This research aimed to obtain the most effective concentration of tapioca flour in development of raw secondary metabolites of Trichoderma harzianum T10, its effect on damping-off and growth of cucumber. This research was carried out at the screen house and the Plant Protection Laboratory, Faculty of Agriculture, Jenderal Soedirman University from September 2017 up to January 2018. The study was conducted in two stages, i.e., in vitro and in planta. The in vitro stage used completely randomized design with five repetitions and five treatments consisted of T. harzianum T10 in Potato Dextrose Broth, and in 0.5; 1; 1.5; and 2% of tapioca flour media. In in planta, randomized block design was used with five repetitions and six treatments consisted of control, T. harzianum T10 in PDB, and in 0.5; 1; 1.5, and 2% of tapioca flour media. Variables observed were density of conidia, disease incubation period, disease incidence, AUDPC, maximum growth potential, germination ability, plant height, canopy fresh weight, root length, and fresh root weight. Result of the research showed that the highest conidial density (1.23 x 107 conidia mL-1) of T. harzianum T10 was found in 2% tapioca flour with an increase of 63.28% compared to the PDB. The tapioca flour of 1 and 2%, and PDB could suppress the disease incidence by 81.82%. The lowest AUDPC was at 2% tapioca flour. The raw secondary metabolites could not delay the incubation period significantly and increase cucumber plant growth. The novelty is the use of antagonistic fungi in terms of raw secondary metabolites and the discovery of tapioca flour with the right concentration to produce high conidia density and high raw secondary metabolites. The benefits are to find other cheaper ingredients in promoting antagonistic fungal growth and the use of antagonistic fungal bioactive compounds to control plant pathogen
Raw Secondary Metabolites of Chitosan-enriched Pseudomonas fluorescens P60 to Control Corn Sheath Blight Soesanto, Loekas; Pradiptha, Clarissa Nadya; Mugiastuti, Endang
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.28775

Abstract

Corn is a strategic cereal with economic value and always face the corn sheath blight in the field. An alternative safely and environmentally friendly disease control is the use of biological agent Pseudomonas fluorescens P60 that produced raw secondary metabolites. This study aimed to test the effectiveness of chitosan enriched P. fluorescens P60 raw secondary metabolites against leaf blight and to determine the effect on the growth of maize. The research was conducted at the Laboratory of Plant Protection and Experimental Farm, Faculty of Agriculture, Jenderal Soedirman University. In vitro test used a completely randomized design and in planta test used a randomized block design consisted of four treatments and six replicates. The treatments consisted of control and the secondary metabolites of P. fluorescens P60 enriched with chitosan 1, 2, and 3%. Variables observed were inhibition ability, chitinase and protease analysis qualitatively, incubation period, disease intensity, infection rate, AUDPC, crop height, crop fresh weight, and root fresh weight. The results showed that the secondary metabolites of P. fluorescens P60 enriched with chitosan 3% was effective in inhibiting the growth of Rhizoctonia solani as 63.3%, produced chitinase and protease, and could control the disease by delaying the incubation period as 79.05%, lowering the disease intensity as 68.68%, lowering the infection rate as 100%, and lowering AUDPC as 83.32%. The secondary metabolites of P. fluorescens P60 enriched with chitosan 3% was effective in improving plant growth by increasing plant fresh weight as 33.9% and root fresh weight as 43.09% compared to control. Chitosan could be used for improving effectiveness of antagonistic bacteria raw secondary metabolites. The raw secondary metabolites of bacterial antagonists could be improved their effectiveness against plant diseases by enrichment of chitosan.
CROSS APPLICATION OF ENTOMOPATHOGENIC FUNGI RAW SECONDARY METABOLITES FOR CONTROLLING FUSARIUM WILT OF CHILI SEEDLINGS Soesanto, Loekas; Sari, Lintang Yunita; Mugiastuti, Endang; Manan, Abdul
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 2 (2021): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.22182-90

Abstract

Cross application of entomopathogenic fungi raw secondary metabolites for controlling fusarium wilt of chili seedlings. Theresearch aimed to determine the effect of entomopathogenic fungi raw secondary metabolites on fusarium wilt on chili plants and on growth of chili. In vitro test used a Completely Randomized Design with 5 treatments and 5 replicate and in planta using a Randomized Block Design with 5 treatments and 5 replicatie including control, secondary metabolites of Beauveria bassiana B10, B. bassiana B16, Metarhizium anisopliae M16, dan Lecanicillium lecanii L16. Variables observed included inhibition ability, incubation period, desease intensity, plant height, root length, and phenolic compounds (tannins, saponin, and hydroquinone) content qualitatively. The results showed that secondary metabolites of B. bassiana B10, B. bassiana B16, M. anisopliae M16, and L. lecanii L16 were able to inhibit growth of Fusarium oxysporum f.sp. capsici by 50.62; 50,64; 48,62; 56.62%, respectively, extend incubation periods of 71.05; 73,38; 64.89; and 68.57%, respectively, suppress disease intensity by 99.99; 99.99; 99.99; and 99.99%, respectively, can increase plant height by 15.22; 18.8; 21.14; 21.69%, respectively, increasing the root length by 22.61; 25,71; 26,34; 33.50%, respectively, and can increase the content of tannins, saponins and hydroquinone compounds qualitatively compared to controls. The secondary metabolites of enthomopathogenic fungi could be used as organic control for soilborne pathogenic fungi.
COGONGRASS ROOT EXTRACT FROM FIVE DIFFERENT SOILS TYPES FOR SUPPRESSING PURPLE BLOTCH AND INCREASING GROWTH AND YIELD OF SHALLOTS Rokhlani, Rokhlani; Soesanto, Loekas; Nur, Subandi; Prihatiningsih, Nur
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 2 (2021): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.221103-115

Abstract

Cogongrass root extract from five different soils types for suppressing purple blotch and increasing growth and yield of shallots. The aim of this study was to examine the efficacy of cogongrass (Imperata cylindrica (L.) Beauv.) root extracts from five different soil types (Typic Udipsamments, Aeric Endoaqualfs (=Gleisal Eutrik), Typic Quartzipsamments (=Regosol Eutrik), Aquertic Chromic Hapludalfs, and Pachic Hapludolls) in suppressing purple blotch and increasing growth and yield of shallots. Split plot design was used with 13 treatments repeated three times, and 18 plants plot-1. The treatments consisted of control, fungicide propineb applied before and after inoculation, and five types of cogongrass root extract 50, 60, and 70% concentration applied before and after inoculation. Results showed that cogongrass root extract collected from Pachic Hapludolls which was applied before inoculation had significant effect on the highest pathosystem component indicated by delaying the incubation period, suppressing the intensity of the disease, slowing down the infection rate, and decreasing values of AUDPC as 41.85, 69.87, 75.13, and 67.63%, respectively, compared to control. The cogongrass root extract from Pachic Hapludolls applied before inoculation could increase plant fresh and dry weight plant-1, tuber weight plant-1, plant fresh and dry weight plot-1, and tuber dry weight plot-1 as 42.7, 49.6, 51.92, 66.75, 72.29, and 73.53%, respectively, compared to control.
Co-Authors . Kustantinah Abdul Manan Aenul Latifah Agus Suyanto Ajeng Faizah Nijma Ilma, Ajeng Faizah Nijma Anik Leana, Ni Wayan Budiyoko, Budiyoko Chalimah, Nur DARINI SRI UTAMI Dwimartina, Fina Endang Mugiastuti Eni Sumarni Ersapoetri, Fida Suci Fakhiroh, Zaqiatul Hanif Nasiatul Baroroh Hiban, Atsil Hidayat, Fitrian Ilahiyyah, Hidayatul Ismi, Sinta Fajriatul Khoeruriza, Khoeruriza Kustam, Kustam Leana, Ni Wayan Anik Muarifah, Mizar Mucharomah, Kurniasih Mugiastuti , Endang Muljo Wachjadi Murti Wisnu Ragil SASTYAWAN Noor Farid Nur Prihatiningsih Nur, Subandi Nuraini, Ika Vidi Okti Herliana, Okti Pradiptha, Clarissa Nadya Prastyani, Noviana Primayuri, Deviana Priswanto Priswanto, Priswanto Purnomo, Widhiatmoko Herry Rahma, Anida Amalia Rokhlani, Rokhlani Rostaman Ruth Feti Rahayuniati Sari, Lintang Yunita Sastyawan, Murti Wisnu Ragil Siti Helmyati SLAMET ROHADI Sundari, Dini Suroto, Agus Woro Sri Suharti Yusup Ziaulhak, Dede Yusup, Adi Maulana Zulkifli, Lutfi