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Comparison of blood clam (anadara granosa) shell paste, shrimp (litopenaeus vannamei) shell paste and casein phosphopeptide-amorphus calcium phosphate (CPP-ACP) paste as teeth remineralization material Asmawati Asmawati; Bahruddin Thalib; Alqarama M. Thalib; Delvi S. Reni; Rafikah Hasyim
Journal of Dentomaxillofacial Science Vol. 3 No. 3 (2018): (Available online: 1 December 2018)
Publisher : DiscoverSys Inc.

Objective: The aim of this study was to determine the comparison of blood clam (anadara granosa) shell paste, shrimp (litopenaeus vannamei) shell paste and casein phosphopeptideamorphus calcium phosphate (CPP-ACP) paste as teeth remineralization material.Material and Methods: This study was an experimental laboratory with cross sectional design. Study sample was 15 maxillary central incisors that were applied with 35% hydrogen peroxide for 2 hours, then assessed with Energy Dispersive Spectroscopy (EDS). Samples were divided into 3 groups: 5 samples were applied using blood clam (anadara granosa) shell paste, 5 samples using shrimp shell paste and 5 samples were applied using CPP-ACP paste every 8 hours for 14 consecutive days then checked with EDS. Data analysis using SPSS test independent t-test to see comparison of blood clam (anadara granosa) shell paste, shrimp (litopenaeus vannamei) shell paste and casein phosphopeptide-amorphus calcium phosphate (CPP-ACP) paste as teeth remineralization material.Results: The result of this study showed P> 0.05 which means there was no significant difference between blood clam shell paste, shrimp shell paste and CPP-ACP mineral composition.Conclusion: Blood clam shell paste and shrimp shell paste has an effective value to be used as tooth remineralization material.

Saliva parameter analysis on smoker and non smoker patients exposed to intraoral radiography Rafikah Hasyim; Aryadi Arsyad; Irene E. Rieuwpassa; Dwi P. Wulansari
Journal of Dentomaxillofacial Science Vol. 4 No. 3 (2019): (Available online: 1 December 2019)
Publisher : DiscoverSys Inc.

Objective: To analyze saliva parameter such as salivary pH, total protein, buffer capacity, as well as sodium and potassium level on smoker and non smoker patients after exposed to intraoral radiography.Material and methods: This study was an observational study with cross-sectional design. Samples were smoker and non smoker patients who were referred to Dental Hospital Hasanuddin University. Saliva samples were taken by draining method. Samples were transferred immediately to Laboratory of Biochemistry, Faculty of Mathematics and Science to be processed regarding saliva pH, saliva total protein, buffer capacity, as well as saliva sodium and potassium level. Saliva pH were measured with pH meter Hanna instruments, total protein with Lowryâ€™s method, buffer capacity with Ericcsonâ€™s method, sodium and potassium level with atomic absorption spectrophotometer Parking Elmer A400. Data were then analyzed with statistical test Kolmogorov Smirnov, independent t-test, and oneway Anova with p<0.05 were considered statistically significant.Result: There were no significant difference on salivary pH, buffer capacity and potassium level between smoker group and non smoker group (p>0.05) before exposed to intraoral radiography, but we found significant difference on saliva total protein and sodium level between smoker and non smoker group (p<0.05) before radiography exposure. There were no significant change on salivary pH, total protein, buffer capacity, sodium and potassium level after exposed to intraoral radiography (p>0.05) on both smoker and non smoker group.Conclusion: Exposure of intraoral radiographs did not have significant effect on salivary pH, total protein, buffer capacity, as well as sodium and potassium level.Â

Analysis of genotoxic and cytotoxic effects of oral mucosa in smokers and non-smokers after exposed to digital intraoral radiography Dwi P. Wulansari; Aryadi Arsyad; Irene E. Rieuwpassa; Nurlindah Hamrun; Rafikah Hasyim
Journal of Dentomaxillofacial Science Vol. 4 No. 3 (2019): (Available online: 1 December 2019)
Publisher : DiscoverSys Inc.

Objectives : To analyze genotoxic and sitotoxic indicators of buccal epithelial cells by measuring the number of cells contain micronucleus, pycnosis, karyorrhexis, karyolysis and to determine the change in oxidative stress ofÂ oral mucosa cells using salivary MDA levels between smokers and nonsmokers before and after exposure to intraoral radiographs during endodontic treatment.Material and Methods : This research was an analytic observation research with cross-sectional design. The sample was divided into smokers (n = 5) and non-smokers group (n = 5). The buccal epithelial cells was taken with cytobrush and unstimulated saliva in each group were taken by the draining method shortly before intraoral radiography, 10Â±2 days after first Â day exposure, 10Â±2 days after second day exposure. The cells were stained using Papanicolau (PAP) kit and observed by pathologist. Genotoxic and cytotoxic indicator measured by counted the number of cells contain mironucleus, pyknosis, karyorrhexis and karyolysis under light microscope with 400X magnification according to the Tolbert criteria in 1000 cells. While oxidative stress of cell measured by salivary MDA with Thiobarbitoric Acid (TBA) assay.Result : The number of micronucleus, pyknosis, karyorrhexis, karyolysis in buccal epithelial cells and salivary MDA levels in smokers was higher than non-smokers (p<0,05). There were no significant differences in genotoxic and cytotoxic indicators and salivary MDA levels before and after the last exposure in smokers and non-smokers (p>0,05).Conclusion : The results of this study indicate that the use of intraoral radiographs is within safe limits despite repeated exposure after several days.

Analysis of first permanent molar condition on Kindergarten student aged 5-6 years old in Eastern Suburban Area of Makassar City Marhamah Marhamah; Rafikah Hasyim; Dwi P. Wulansari; Muliaty Yunus; Hendrastuty Handayani; Sumintarti Sumintarti; Sri Ramadhany
Journal of Dentomaxillofacial Science Vol. 5 No. 1 (2020): (Available online: 1 April 2020)
Publisher : DiscoverSys Inc.

Objective: The aim of this study was to analyze the first permanent molar condition on kindergarten student aged 5-6 years old in eastern suburban area of Makassar city.Material and method: This study was a descriptive observational study on 100 kindergarten students from four schools in Tamalanrea District, Makassar City. Data was collected with clinical examination and interview. Data was gathered in terms of DMFT status of first permanent molar and OHI-S. Data was analyzed with descriptive statistics and t-test.Â In this descriptive cross-sectional study. Statistical analysis was performed by descriptive statistics and t-test.Results:Â Mean DMFT of first permanent molars was 1.00 Â± 1.10, with a higher value seen among boys. Mean decayed teeth component was 0.95, followed by filled (0.10) and missing (0.0) components.Â Conclusion:Â The observed oral hygiene status among the 5-6Â year-olds necessitates implementing pre-ventive as well as restorative measures to improve the oral health status of the young population.

The increase of fibroblast cells number in rat (rattus norvegicus) gingival wound after the application of moringa (moringa oleifera lam) fruit oil Asmawati; Bahruddin Thalib; Nurhayaty Natsir; Alqarama M. Thalib; Rafikah Hasyim
Journal of Dentomaxillofacial Science Vol. 5 No. 3 (2020): (Available online: 1 December 2020)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v5i3.1121

Objective: To determine the increase of fibroblast cell number in the rat (Rattus norvegicus) gingival wound after the application of moringa (Moringa oleifera Lam) fruit oil.Material and Methods: This study was an experimental post-test control group design using 18 male Wistar rats (Rattus norvegicus) that was divided into three groups. The sample was previously incised with a length of 4 mm using scalpel 15. Group 1 was applied with moringa fruit oil, group 2 was given aspirin, and group 3 was given at 0.5% CMC. The application was carried out every 24 hours, then on the third and seventh day, the rats were euthanized for histological preparations and then observed under the electric microscope. The length of the lesion was measured on the first, third, and seventh days. The fibroblast was then observed by counting the number of fibroblast cells per field of view using an Olympus C-21 microscope with an Optilab Advances camera with a 40x, 100x magnification, and 400x. The data were processed and analyzed using the SPSS Version 21 program. This study used a t-test to see the differences between the treatment group and the control group, and the Least Significantly Difference (LSD) test was used to see the size of the difference.Results: This study showed a significant increase in the number of fibroblasts in rat gingival mucosa on the third and seventh daysConclusion: Moringa (Moringa oleifera Lam) fruit oil affects increasing the number of fibroblasts on the rat gingival mucosa (Rattus norvegicus).

Compressive and tensile strength measurement of irreversible hydrocolloid impression material made of brown algae Padina sp Nurlindah Hamrun; Rafikah Hasyim; Acing H. Mude; Muhammad Ikbal; Teiza Nabilah; Mutiaranisa Safitri; Fuad H. Akbar
Journal of Dentomaxillofacial Science Vol. 6 No. 1 (2021): (Available online: 1 April 2021)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v6i1.1190

Objective: This study aimed to explore the potential of brown algae (Padina sp) by measuring its compressive and tensile strength as a preparation step for making standardized dental impression materials from sodium alginate Padina sp.Methods: This study was a quasi-experimental study with one-shot case design. Brown algae were taken from the waters of Punaga and Puntondo, Takalar. Sodium alginate was extracted from algae then mixed with other compositions to form alginate impression material. Compressive and tensile were tested using the Point Load Test.Results: Compressive strength of impression material made of Padina sp was higher than that of standard impression material with pressure mean 0.011 Mpa and 0.009 Mpa. The mean tensile strength measurement was 0.003 Mpa.Conclusion: Mean compressive and tensile strength of impression material made of Padina sp were still relatively weak.

Salivary pH, dental caries and Body Mass Index (BMI) in the middle school students in Eastern Makassar Rafikah Hasyim; Dwi P. Wulansari; Karima Q. Mansjur; Nasyrah Hidayati
Journal of Dentomaxillofacial Science Vol. 6 No. 3 (2021): (Available online: 1 December 2021)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v6i3.1278

Objective: This study aimed to assess the association of salivary pH, dental caries and body mass index (BMI) in middle school students in eastern MakassarMaterial and Methods: This study included 60 middle school students from two schools in eastern Makassar. Data were collected by filling out a questionnaire, weight and height measurement, dental examination, and saliva collection. Body mass index was calculated by dividing weight and height in kg/m2, saliva was collected by draining method, and salivary pH was measured with Saliva-Check Buffer (GC Japan). Decay Missing Filled-Teeth (DMF-T) indexes are used to assess dental caries. Statistical analysis was performed by chi-square and ANOVA with a significance of 0.05.Results: This study showed that overweight and obesity are more prevalent in female students than male students. Salivary pH in underweight students and overweight and obese students were lower compared to normal BMI students, while DMF-T score in underweight, overweight, and obese students were higher than the normal BMI students.Conclusion: It can be concluded that BMI have a significant association with salivary pH and DMF-T score.1. Wang L, Zhou B, Zhao Z, et al. Body-mass index and obesity in urban and rural China: findings from consecutive nationally representative surveys during 2004–18. Lancet 2021;398: 53-63.2. Garrouste-Orgeas M, Troché G, Azoulay E, et al. Body mass index. Intensive Care Med 2004;30: 437-443.3. Barlian B, Azhari, Pramanik F. Correlation between mandibular trabeculae bone density on panoramic radiograph and body mass index of men aged 5-35 years old. J Dentomaxillofac Sci 2018;3: 166-168.4. Bellagambi FG, Lomonaco T, Salvo P, et al. Saliva sampling: Methods and devices. An overview. TrAC-Trends Anal Chem 2020;124: 115781.5. Zalewska A, Kossakowska A, Taranta-Janusz K,et al. Dysfunction of salivary glands, disturbances in salivary antioxidants and increased oxidative damage in saliva of overweight and obese adolescents. J Clin Med 2020;9: 548.6. Mosca AC, Stieger M, Neyraud E, et al. How are macronutrient intake, BMI, ethnicity, age, and gender related to the composition of unstimulated saliva? A case study. J Texture Stud 2019;50: 53-61.7. Pannunzio E, Amancio OMS, Vitalle MSDS, et al. Analysis of the stimulated whole saliva in overweight and obese school children. Rev Assoc Méd Bras 2010;56: 32-36.8. Primasari A, Hulu KP. Dryness of the oral cavity in the salivary status of overweight women. J Homeop Nat Med 2019;5: 50-53.9. Al-Hussaini A, Bashir MS, Khormi M, et al. Overweight and obesity among Saudi children and adolescents: Where do we stand today?. Saudi J Gastroenterol 2019;25: 229.10. Hruby A, Hu FB. The epidemiology of obesity: A big picture. Pharmaco Economics 2015;33: 673-689. 11. Alshihri AA, Rogers HJ, Alqahtani MA, et al. Association between dental caries and obesity in children and young people: A Narrative Review. Int J Dent 2019;2019: 1-8. 12. Groth SW, Morrison-Beedy D. Obesity risk in urban adolescent girls: nutritional intentions and health behavior correlates. J NY State Nurses Assoc 2011;42: 15-28.13. Baliga S, Muglikar S, Kale R. Salivary pH: A diagnostic biomarker. J Indian Soc Periodontol 2013;17: 461.14. Anamaria B, Eugen B, Daniela E, et al. Interrelation between salivary pH, buffer capacity and dental caries in underweight, normal weight and overweight children. Revista de Chimie 2017;68: 1353-1356.

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