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All Journal Journal of Applied Food Technology Jurnal Teknologi Pangan
Purwitasari, Lutfi
Department Food Technology, Faculty Of Animal And Agricultural Sciences, Diponegoro University, Indonesia
Veterinary Other

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Journal : Journal of Applied Food Technology

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Review on Pathogenic Bacteria Listeria monocytogenes, the Detection and the Sequencing Gene Methods Isolated from Meat Products Bhakti Etza Setiani; Yoyok Budi Pramono; Lutfi Purwitasari
Journal of Applied Food Technology Vol 6, No 2 (2019)
Publisher : Dept. Food Technology, Faculty of Animal and Agricultural Sciences, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1099.242 KB) | DOI: 10.17728/jaft.6460

Abstract

A study was conducted to review on pathogenic bacteria Listeria monocytogenes, the detection and the sequencing gene methods isolated from meat products, compare selected methods that detect the presence of L. monocytogenes in selected raw and processed meat products. L. monocytogenes (originally named Bacterium monocytogenes) is a gram-positive, non-sporeforming, highly mobile, rod-type, and facultative anaerobic bacterium species. It can grow under temperatures between -1.5 to 45°C and at pH range between 4.4 and 9.4, with the optimum pH of 7. Rapid methods (PCR based and VIDAS-LDUO®) detected L. monocytogenes faster than the conventional method. It was also gathered that Phenotypic identification and Genotypic identification were two types of confirmation test for L. monocytogenes. L. monocytogenes can be found in raw meat and meat product because of environmental contamination, cross contamination or error process.
Mechanisms and Applications of Essential Oils as Natural Preservatives in Meat Products: A Review Afidah, Ulil; Wardhani, Riuh; Dewi, Swastika; Purwitasari, Lutfi
Journal of Applied Food Technology Vol 12, No 2 (2025)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17728/jaft.29970

Abstract

The increasing global demand for meat products illustrates the importance of safe and effective preservation methods. Butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), and tertiary butylhydroquinone (TBHQ) are common synthetic preservatives that stop microbial growth and lipid oxidation. However, they have been linked to negative health effects. Consequently, natural alternatives such as essential oils (EOs) have gained attention due to their antimicrobial and antioxidant activities. Mechanistically, EOs disrupt microbial cell membranes, inhibit key metabolic enzymes, and scavenge free radicals that initiate lipid oxidation. This review discusses the mechanisms and applications of various essential oils as natural preservatives in meat and meat products. Essential oils, which are high in phenolic compounds, work by breaking down microbial cell membranes, stopping enzymatic systems, and neutralizing free radicals that cause oxidation. Several studies have demonstrated that essential oils (EOs) function as a promising natural preservative in meat products by utilizing specific mechanisms to enhance chemical, microbiological, and sensory quality. For instance, rosemary essential oil (REO), when integrated into vacuum packaging, effectively extends the shelf life of chicken meatballs by inhibiting microbial growth and scavenging free radicals to prevent lipid oxidation. Similarly, oregano essential oil (OEO) exhibits strong antimicrobial action against foodborne pathogens like Salmonella enteritidis and Escherichia coli in dried meat while enhancing sensory properties. In sausage products, nutmeg essential oil (NO) demonstrates its antioxidant capacity by significantly reducing lipid oxidation indicators such as thiobarbituric acid reactive substances (TBARS), suppressed mesophilic bacteria growth, and improved aroma stability during storage. Collectively, these findings confirm that essential oils represent promising natural preservatives that offer a safer multi-target alternative to synthetic additives, especially in the mechanism of cell membrane damage and lipid peroxidation inhibition.
Co-Authors Afidah, Ulil Bambang Dwiloka Bhakti Etza Setiani Swastika Dewi Wardhani, Riuh Yoyok Budi Pramono