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ISOLASI DAN SELEKSI BAKTERI ASAM LAKTAT DARI YOGHURT PRODUKSI INDUSTRI RUMAH TANGGA DI PEKANBARU YANG BERSIFAT ANTIBAKTERI TERHADAP Escherichia coli DAN Salmonella typhi Lili Yani; Rodesia Mustika Roza; Atria Martina
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 1 (2014): Wisuda Februari 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

The occurance of Lactic Acid Bacteria (LAB) in yoghurt produced by home industry inPekanbaru as probiotic potentially improve intestinal physiological function, useful forhealth as well as prevention of the growth of pathogenic bacteria. The purposes of thisstudy were to calculate the total LAB population of yoghurt with the effect of mango andstrawberry juice during different storage process, and also to determine the ability ofantibacterial activity against Escherichia coli and Salmonella typhi. The population of LABin yoghurt was 4,0 × 10 7 – 2,9 × 10 8 CFU/ml. Antimicrobial activity assay used the welldiffusion method. The mango juice yoghurt incubated in 7 day was the most effective forEscherichia coli with the inhibition zone 27,1 mm. While the mango juice yoghurtincubated in 3 day was the most effective for Salmonella typhi with inhibition zone 29,9mm.
UJI POTENSI ANTIFUNGI AKTINOMISETES SELULOLITIK DAN LIGNINOLITIK DAN BAKTERI LIGNOSELULOLITIK ISOLAT LOKAL TERHADAP PERTUMBUHAN JAMUR Ganoderma boninense DAN Colletotrichum capsici Dede Martin; Atria Martina; Rodesia Mustika Roza
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Colletotrichum capsici fungi, causing agent of anthracnose in chili, and Ganoderma boninense, fungi causing agent of basal stem rotten in oil palm, can reduce the productivity of chili and oil palm. These disease are increasing so that it is necessary to find local biological agents that are environmentally friendly. The purpose of this research was to determine the potential isolates of lignocellulolyltic bacteria and cellulolytic and ligninolytic actinomycetes from peat soil of Rimbo Panjang Kampar, Riau as an antifungal agent to inhibit the growth of C. capsici and G. boninense.Antifungal activity was screened using agar disc method by measured the inhibition zone for seven days. The results showed that 13 isolates of actinomycetes haveantifungal activity against C. capsici with the highest inhibition zone 13,3 mm by RB2S40. Six isolates of actinomycetes have antifungal activity against G. boninense with the highest inhibition zone 29,15 mm by RB1S4. Five isolates have the ability to inhibit both fungi which were targeted.
ANALISIS FISIOLOGI BAKTERI LIGNOSELULOLITIK DAN AKTINOMISETES SELULOLITIK DAN LIGNINOLITIK DARI TANAH GAMBUT DESA RIMBO PANJANG KABUPATEN KAMPAR SEBAGAI AGEN BIOKOMPOS Novia Lestari; Rodesia Mustika Roza; Atria Martina
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

During a composting process, microorganisms such as bacteria, fungi, and actinomycetes have a role in degradation of organic matter. Temperature and pH also influence composting process. This research was aimed to obtain bacterial isolates and actinomycetes with the best ability to be applied as biocompost agent by analyzing the ability of those isolates to grow in different temperature and pH. Isolates of actinomycetes and bacteria were isolated from peatsoils in Rimbo Panjang, Kampar Regency, Riau. Bacterial isolates were inoculated into Nutrient Broth medium and actinomycetes isolates were inoculated into Starch Casein Broth medium. The cultures were incubated at room temperature (±27°C), 40°C, 50°C, 60°C, 70°C and 80°C in pH of 5 and pH of 7. The results showed that 3 isolates of bacteria and 90 actinomycetes isolates were able to grow at room temperature (±27°C) and 40°C in pH of 5 and pH of 7. A total of 28 actinomycetes isolates grew at 50°C in pH 5 and pH 7 with 2 isolates were considered as the most potential, which were Rhodococcus sp. and Streptomyces sp..
AKTIVITAS ANTIBAKTERI EKSTRAK DAUN Cyclea barbata (L.)Miers. TERHADAP BAKTERI Escherichia coli DAN Salmonella typhi Arifan Asmardi; Rodesia Mustika Roza; Fitmawati '
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Indonesia has many medicinal plant species that are distributed in many regions. Green grass jelly (Cyclea barbata (L.) Miers.)is one of medicinal plant that used in traditional medicine by Indonesian people. This species used to treat inflammation, fever and others. The main content of green grass jelly are saponins, flavonoids and alkaloids. Escherichia coli and Salmonella typhi can infect the human body, but the green grass jelly can inhibit the growth of these bacteria. This study was performed using four methods, maceration, fresh leaf grinding, fresh and dried leave boiling, the dilution series (10%, 25%, 50%, 75% and 100%) were applied for each method. The best result among four methods was the maceration method with 100% concentration dilution. The treatment using E. coli showed the widest diameter of inhibition zone was 17.23 mm and the lowest diameter  was 7,70 mm. while the treatment using S. typhi showed the widest diameter was 15.89 mm and the lowest diameter was 6,85 mm. The higher  the concentrations used, the wider the inhibition zone diameter was.
The Activities of Plant Extracts Heritage of Melayu Culture-Riau Archipelago on Bacteria Causing Diarrhea Rodesia Mustika Roza
International Journal of Ecophysiology Vol. 1 No. 1 (2019): International Journal of Ecophysiology
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (307.319 KB) | DOI: 10.32734/ijoep.v1i1.842

Abstract

Diarrhea is one of the major health problems in Indonesia. Lingga Malay ethnic in Riau archipelago use alternative medicines in a form of a obat pahit potion to treat diarrhea. This research aimed to determine the antibacterial activity of obat pahit extraction against Escherichia coli ATCC 11775, Salmonella ATCC 14028 and Shigella flexneri ATCC 12022. Antibacterial activity can be seen with the formation of inhibitory zone using diffusion method. The extract concentration used were 100%, 75%, 50% and 25%. Based on the antibacterial activity test, the largest inhibitory zone against E. coli was found at 100% Cnestis palala extract of 8.29 mm and the smallest inhibitory zone was found at 25% traditional medicine practioner (TMP) 2 SP4 of 5.73 mm. The more over, the largest inhibitory zone to S. was found at 100% Bauhinia semibifida of 8.81 mm; and the smallest inhibitory zone was found at 50% TMP 2 SP4 of 6.30 mm. Antibacterial activity with the largest inhibitory zone against S. flexneri at 75% C. palala of 6.21 mm and the smallest inhibitory zone was found at 50% TMP 2 SP4 of 5.62 mm.
Inovasi teknologi hidroponik melalui pemanfaatan barang bekas dan nutrisi buatan sendiri sebagai usaha peningkatan pendapatan petani di Desa Kualu Kecamatan Tambang Kabupaten Kampar Fitmawati Fitmawati; Mayta Novaliza Isda; Isnaini Isnaini; Nery Sofiyanti; Rodesia Mustika Roza
Unri Conference Series: Community Engagement Vol 1 (2019): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.1.499-505

Abstract

The hydroponic technology of the wick system is one of the innovations in vegetable cultivation without soil with the innovation of utilizing used goods and household waste such as styrofoam and used cans as a container for planting. The purpose of this activity is to reduce waste in the community, improve the family's economic capacity, and people's interest in consuming clean and hygienic vegetables in Kualu Village, Tambang District, Kampar Regency. This hydroponic technique is made of relatively inexpensive and affordable materials so that the limitations of people's financial capabilities can be overcome. This system is a hydroponic system that uses axes to absorb organic nutrients. To minimize the cost of purchasing nutrients, it can be overcome by innovating nutrient solutions from household waste into liquid fertilizer. The implementation of community service uses counselling, discussion and making hydroponic technology. The community is very enthusiastic in joining this program, especially housewives who want to know the application of Wick's hydroponic technology so that they can play a large role in meeting food needs and increasing people's income.
Aktivitas Antibakteri Kombinasi Ekstrak Mangifera laurina, Ruellia tuberosa L. dan Leucobryum sp. pada Luka Penderita Diabetes Rodesia Mustika Roza; Fitmawati; Hari Kapli; Fitra Suzanti
Prosiding Workshop dan Seminar Nasional Farmasi Vol. 1 (2022): Prosiding Workshop dan Seminar Nasional Farmasi 2022
Publisher : Program Studi Farmasi Fakultas MIPA Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/WSNF.2022.v01.i01.p02

Abstract

Mangifera laurina termasuk salah satu mangga liar di Sumatra yang memiliki kandungan antioksidan, fenol dan flavonoid yang tinggi. Tanaman Ruellia tuberosa L dan Leucobryum sp. diketahui memiliki sifat antibakteri. Perpaduan tiga tumbuhan ini diharapkan dapat menjadi solusi dalam mengobati luka pada penderita diabetes menggantikan antibiotik, karena penggunaan antibiotik yang tidak tepat dapat menyebabkan bakteri menjadi resisten. Tujuan penelitian ini adalah menguji aktivitas ekstrak metanol kombinasi tiga jenis tanaman (Mangifera laurina, Ruellia tuberosa L dan Leucobryum sp) terhadap Staphylococcus aureus ATCC 6538 dan S. epidermidis ATCC 12228. Metode ekstraksi yang digunakan adalah maserasi dengan metanol 70%. Pengujian aktivitas antibakteri menggunakan konsentrasi ekstrak sebesar 10% dengan metode kertas cakram pada medium Mueller Hinton Agar (MHA), diinkubasi pada suhu 37°C selama 24 jam. Kontrol positif yang digunakan adalah antibiotik kloramfenikol 30µg/disc dan DMSO 10% sebagai kontrol negatif. Hasil zona hambat yang terbentuk dibandingkan dengan Clinical and Laboratory Standards Institute (CLSI). Hasil uji kombinasi ekstrak M. laurina, R. tuberosa L dan Leucobryum sp. terhadap S. aureus ATCC 6538 diperoleh kisaran zona hambat 7,55±0,35-10,05±0,54 mm. Zona hambat terbesar pada perlakuan P2 (perbandingan 3:2:3) dan zona hambat terkecil pada perlakuan P5 (perbandingan 2:1:3). Zona hambat yang terbentuk terhadap S. epidermidis ATCC 12226 berkisar 7,88±0,11-8,28±0,81 mm. Zona hambat terbesar pada perlakuan P6 (perbandingan 1:3:2) dan yang terkecil perlakuan P5 (perbandingan 2:1:3). Pada perlakuan P1-P4 tidak terbentuk zona hambat. Berdasarkan CLSI, zona hambat yang terbentuk ≤ 12 mm termasuk kategori resisten. Zona hambat kloramfenikol terhadap S. aureus termasuk kategori sensitif, akan tetapi terhadap S. epidermidis termasuk kategori resisten. Kombinasi ekstrak metanol Mangga liar M. laurina, R. tuberosa dan Leucobryum sp. berpeluang sebagai kandidat antibiotik.
Antibacterial Activity Test of Endophytic Microbes of Bird's Nest Fern (Asplenium nidus) Against Propionibacterium acnes Bacteria Roza, Rodesia Mustika; Wardani, Cloudia Pramesti Kusuma
Jurnal Biologi Tropis Vol. 25 No. 4a (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4a.10890

Abstract

Acne is a widespread skin treatment frequently seen within Indonesia. This condition, also known as Acne vulgaris, arises from an infection or irritation affecting the pilosebaceous units. This is caused by obstructions and build-up of keratin, which are spurred on by the existence of Propionibacterium acnes bacteria. Current acne treatment uses a lot of synthetic antibiotics, as a result if used for a long period of time it can increase resistance so that acne is difficult to cure. One effort to overcome drug resistance is to utilize endophytic bacteria that have the ability to produce similar compounds from their host plants including antibacterial compounds. Plants that have the potential as antibacterials are bird's nest ferns (Asplenium nidus). The content of this plant is flavonoids, alkaloids, terpenoids, phenolics, tannins, and saponins. This research intends to evaluate the antibacterial properties of endophytic microorganisms derived from the A. nidus plant against P. acnes bacteria. The assessment of antibacterial capabilities was performed through the utilization of the agar disk diffusion technique. The separation process resulted in a total of 16 endophytes, which were further categorized into 11 bacterial strains and 5 fungal strains. Evaluation of the antibacterial properties of the endophytes revealed that 9 bacterial endophyte strains could impede the proliferation of P. acnes, with strain BRAN 10 exhibiting the greatest antibacterial effect, characterized by a zone of inhibition measuring 13.47 mm in diameter. Isolate JDAN 3 demonstrated the most potent antifungal activity among the endophytes, marked by an inhibition zone diameter of 9.9 mm.