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Seleksi Aktinomisetes Penghasil Protease dari Tanah Gambut Desa Langkai, Siak, Riau LINDA, TETTY MARTA; MARTINA, ATRIA; FEBRIANTI, BERNADETA LENI; , HERLINDA; , TABRI
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

Actinomycetes is well known as secondary metabolites producer and hence it has high pharmacological andcommercial interest. In this study, a total of 14 isolates of actinomycetes from peat soils in Langkai Village,Siak, Riau had been selected for their ability to produce protease. Extracellular protease from Actinomycetesisolates was characterized by incubating the isolate in Nutrient Agar media containing casein 1%. Screeningof actinomycetes isolates showed that five isolates had the ability to produce a clear zone with the value ofproteolytic index (IP) ranged from 5.73 to 11.15. Isolates L313 showed the highest IP value (11.15), as wellas the highest protease production (0.041 U/ml) in Nutrient Broth media containing casein 1% after 6 daysincubation at room temperature.Key words: actinomycetes, clear zone, peat soil, protease, proteolitic index
Uji Patogenisitas Fungi Entomopatogen Lokal Riau sebagai Agen Biokontrol Hama Rayap (Coptotermes curvignathus Holmgren) SINTAWATI, REGIANA; MARTINA, ATRIA; LINDA, TETTY MARTA
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

Biopesticide is a technique that can be used to the control pest population in the environment. Thepurpose of this research was to isolate fungi from the soil using baiting and cadaver technique andpathogenicity test. Isolates which had high germinating conidia was tested their patogenicity againstCoptotermes curvignathus. A total of 12 isolates were baited from soil and cadaver samples at palm oilplantation. The result showed eight isolates had high germinating conidia categories (˃ 80%). Thepathogenicity test of four isolates showed 100% mortality for RB.r01 and BK.r01; and KB.r01 andNK.r01were 72% and 67% respectively. Isolates capability against termite was not significantly different,but it was significantly different from control.Keywords : entomopathogenic fungi, pathogenicity, Coptotermes curvignathus, biocontrol
Seleksi Isolat Aktinomisetes Asal Tanah Gambut Desa Rimbo Panjang Kabupaten Kampar Dalam Menghasilkan Hormon IAA (Indole Acetic Acid) MAWARTI, INDAH; FIBRIARTI, BERNADETA LENI; ZUL, DELITA; ROZA, RODESIA MUSTIKA; MARTINA, ATRIA; LINDA, TETTY MARTA
Jurnal Riau Biologia Vol 2, No 1 (2017)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

Hormon IAA (Indole Acetic Acid) termasuk fitohormon golongan auksin yang berperan sebagai zat pemacu pertumbuhan tanaman. Selain tumbuhan, mikroba juga diketahui mampu menghasilkan IAA. Penelitian ini bertujuan untuk menyeleksi kemampuan koleksi isolat aktinomisetes Laboratorium Mikrobiologi FMIPA UR dalam menghasilkan IAA. Uji produksi IAA oleh aktinomisetes dilakukan dengan penambahan reagen Salkowski menggunakan metode kolorimetri dalam medium SCB (Starch Casein Broth) yang diperkaya dengan triptofan sebagai prekursor dan tanpa triptofan. Hasil penelitan ini diperoleh sebanyak 50 dari 85 isolat aktinomisetes yang memiliki kemampuan dalam menghasilkan hormon IAA. Produksi IAA tertinggi dihasilkan oleh RB5S78 sebesar 35,97 ppm dan konsentrasi terendah dihasilkan oleh RB4S67 sebesar 0,24 ppm dalam medium SCB yang diperkaya triptofan. Pada medium tanpa triptofan hasil tertinggi diperoleh isolat aktinomisetes RB5S78 dengan konsentrasi sebesar 15,28 ppm dan konsentrasi terendah dihasilkan oleh RB4S65 sebesar 0,16 ppm. Isolat aktinomisetes yang menghasilkan IAA dengan kriteria tinggi yang diperoleh termasuk dalam genus Streptomyces dan Rhodococcus.
KEMAMPUAN ISOLAT TUNGGAL DAN KONSORSIUM AKTINOMISETES LOKAL RIAU DALAM MENDEGRADASI HIDROKARBON MINYAK BUMI Novalita Dwi Fanny; Tetty Marta Linda; Atria Martina
BIO-SITE |Biologi dan Sains Terapan Vol. 4 No. 2 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (532.94 KB) | DOI: 10.22437/bs.v4i2.5691

Abstract

Pollution of crude oil on environmental can have an effect of nutrient uptake on plants and balance of ecosystems. Biodegradation. is one way to reduce petroleum pollution . The purpose of this research are determine the potential growth of actinomycetes local Riau (L11 and L121) in crude oil hydrocarbons at concentrations of 0%, 2%, 5% and to know their ability to degrade crude oil hydrocarbons at concentrations of 5%. This research observed the growth of each actinomycetes local Riau and the percentage of crude oil degradation by a single isolate and a consortium with gravimetric method. The results showed that the growth of L11 and L121 total population was not significantly different at addition crude oil of 2% and 5% but significantly different wth 0% (without crude oil). Test degradation of crude oil using single isolate gave significantly different results for the consortium isolates. Percentage of degradation crude oil by L11 was 23.5%, L121 was 19.2% and consortium isolate (L11 + L121) was 31.9% with an incubation time of 10 days in a minimum medium salts. This actinomycetes local Riau can be developed as biodegradation agents for crude oil on soil and water.
Aktivitas Ligninolitik Beberapa Jamur Aphyllophorales dan Kemampuannya Mendegradasi Lignin pada Lindi Hitam Atria Martina; Tetty Marta Linda; Delita Zul; Nila Veronika; Ratna Jelita
Al-Kauniyah: Jurnal Biologi Vol 8, No 1 (2015): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v8i1.2702

Abstract

Fourteen local isolate Aphyllophorales fungi were screened their ligninolytic activity. The isolate with highest ligninolytic activity was tested it capability to degrade kraft blackliquor lignin. The biodegradability of black liquor is low because the presence of lignin and lignin derivative in the wastewater. These fungal were screened for ligninolytic activity by decolorization on solid mediacontaining RBBR dye. The ability of the fungal strains to biodegrade kraft black liquor lignin was performed by submerged fermentation condition with agitation and incubation time as treatment. The solid culture result in 3 isolates had ligninolytic activity and Ganoderma sp.BTA1 gave the highest ligninolytic. Agitation and incubation time influenced ligninbiodegradation of blackliquor significantly. Optimum condition for lignin biodegradation was at 200 rpm during 25 days with lignin reduction was 45,786%.
POTENSI JAMUR INDIGENUS RIAU (Penicillium sp.PN6) DAN Neptunia oleracea UNTUK BIOREMEDIASI OIL SLUDGE Wahyu Lestari; Atria Martina; Rodesia Mustika Roza; Imelda Wardani
Al-Kauniyah: Jurnal Biologi Vol 11, No 1 (2018): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (607.863 KB) | DOI: 10.15408/kauniyah.v11i1.6350

Abstract

Abstrak Lumpur minyak bumi (oil sludge) memiliki kandungan kontaminan petroleum hidrokarbon terbesar, karenanya pengolahan limbah oil sludge perlu dilakukan agar tidak mencemari lingkungan. Penelitian yang menguji kemampuan jamur indigenus Riau (Penicillium sp. PN6) dan legum akuatik (Neptunia oleracea) dalam meremediasi senyawa hidrokarbon dengan pengukuran total petroleum hidrokarbon (TPH) pada limbah oil sludge telah dilakukan. Pertumbuhan N. oleracea dilakukan pada media limbah oil sludge dengan konsentrasi 25, 50, 75, dan 100%. Selanjutnya dilakukan pemberian inokulum Penicillium sp. PN6 ke dalam media pertumbuhan. Hasil penelitian menunjukkan bahwa persentase pertumbuhan N. oleracea adalah 95,58; 85,87; 25,55; dan 0%, berturut-turut pada limbah oil sludge 25, 50, 75, dan 100%. Terjadi penurunan konsentrasi TPH awal pada semua perlakuan di akhir pengamatan. Penicillium sp. PN6 meningkatkan degradasi TPH dari limbah oil sludge dibandingkan dengan kombinasi Penicillium sp. PN6 dengan N. oleracea, tetapi kombinasi Penicillium sp.PN6 dengan N. oleracea meningkatkan persentase penurunan TPH dari limbah oil sludge dibandingkan dengan menggunakan Penicillium sp. PN6 saja. Penicillium sp. PN6 dan kombinasinya dengan N. oleracea sama-sama mampu menurunkan pH limbah oil sludge.Abstract Oil sludge contains the largest contaminant of petroleum hydrocarbon that needs treatment to prevent its pollution into the environment. A study examining the ability of a Riau indigenous fungi (Penicillium sp. PN6) and an aquatic legume (Neptunia oleracea) to remediate hydrocarbon compounds by determining total petroleum hydrocarbon (TPH) in oil sludge waste has been conducted. The growth of N. oleracea was performed in the oil sludge waste media by concentrations of 25, 50, 75, and 100%. The medium was inoculated with Penicillium sp. PN6. The results showed that the percentage of growth of N. oleracea were 95.58; 85.87; 25.55; and 0%, in the oil sludge waste of 25, 50, 75, and 100%, respectively. There was a decrease in initial TPH concentration at all treatments at the end of the observation. Penicillium sp. PN6 increased more TPH degradation of the oil sludge waste compared to a combination of Penicillium sp. PN6 and N.oleracea, but the combination increased the decrease percentage of TPH compared to those that used Penicillium sp. PN6 only. Penicillium sp. PN6 and its combination with N. oleracea are both able to decrease pH of the oil sludge waste. Permalink/DOI: http://dx.doi.org/10.15408/kauniyah.v11i1. 6350 
ANTAGONISTIC TEST OF RIAU LOCAL FUNGAL ISOLATES AGAINST SOME PATHOGENIC IN CULTIVATED PLANTS Nia Safitri; Atria Martina; Rodesia Mustika Roza
Al-Kauniyah: Jurnal Biologi Vol 12, No 2 (2019): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v12i2.8730

Abstract

Tanaman budi daya merupakan tanaman yang sering diserang oleh cendawan pathogen, sehingga mengakibatkan penurunan populasi dan produksi tanaman. Pengendalian hayati dengan cendawan antagonis merupakan salah satu metode yang paling efektif dan lebih ramah lingkungan dalam menekan pertumbuhan patogen tanaman. Penelitian ini bertujuan untuk menguji aktivitas antagonis cendawan isolat lokal Riau terhadap beberapa cendawan patogen pada tanaman budi daya. Uji antagonis dilakukan secara in vitro dengan metode dual culture menggunakan lima belas cendawan isolat lokal Riau terhadap Fusarium oxysporum f.sp. lycopersici, Ganoderma philippii, G. boninense, Rigidoporus microporus dan Colletotrichum sansevieria. Hasil penelitian menunjukkan bahwa Trichoderma sp. PNE 4 memiliki aktivitas antagonis tertinggi dan isolat FER C1 serta isolat LLB07 hanya memiliki aktivitas antagonis yang tinggi dalam menekan pertumbuhan cendawan patogen. Trichoderma sp. PNE 4 mampu menghambat pertumbuhan miselium F. oxysporum sebesar 85,30%, G. Philippii (100%), G. boninense (100%), dan C. sansevieria (100%). Isolat FER C1 hanya menghambat R. Microporus (50,39%) dan isolat LLB07 menghambat G. philippii (52,20%). Trichoderma sp. PNE 4 merupakan cendawan uji yang terpilih sebagai cendawan antagonis, karena memiliki kemampuan daya hambat  >70%.Abstract Cultivated plants are often attacked by pathogenic fungi resulting in a decline of population and crop production. Biocontrol with antagonistic fungi is one of the most effective and environmentally friendly methods in suppressing the growth of plant pathogens. This study aims to examine the antagonistic activity of local isolates fungi Riau against some pathogenic fungi on cultivated plants. The antagonistic test was performed in vitro by dual culture method using fifteen local isolates fungal Riau against Fusarium oxysporum f.sp. lycopersici, Ganoderma philippii, G. boninense, Rigidoporus microporus and Colletotrichum sansevieria. The results showed that Trichoderma sp. PNE 4 isolate exhibited highest activites and  FER C1 and LLB07 isolates exhibited high activities suppressed the growth of the fungal pathogen. Trichoderma sp. PNE 4 isolate inhibited mycelial growth F. oxysporum (85.30%), G. philippii (100%), G. boninense (100%) and C. sansevieria (100%). FER C1 isolate only inhibited R. microporus (50.39%), and LLB07 isolate inhibited G. philippii (52.20%). Trichoderma sp. PNE 4 isolate is test isolates as fungal antagonistic.
Pendugaan laju sedimentasi pada kolam tanah budidaya ikan patin intensif di Desa Koto Mesjid Kecamatan XIII Koto Kampar Saberina Hasibuan; Syafriadiman Syafriadiman; Atria Martina; Henni Syawal; Rinaldi Rinaldi
Riau Journal of Empowerment Vol 2 No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (947.981 KB) | DOI: 10.31258/raje.2.2.71-80

Abstract

The potential of fisheries in Koto Mesjid village XIII Koto Kampar districts has had a tremendous impact on people's income. The total area of ​​the Patin pond in Koto Mesjid village has reached 62 hectares. The production reaches six tons per day. Training in prediction the rate of sedimentation in intensive catfish aquaculture ponds in the village of Koto Mesjid has resulted in a way to monitor the quality of pond water through the measurement of suspended solids using a solid trap device. The average suspended solids at all pond ages are 19.5 ml / L per day and 129.25 ml / L per week. Based on this data, the management of the pond bottom must be carried out every harvest, mainly when drying the pond needs to drain mud. Patin fish farmers have very high enthusiasm to practice the tools in their respective ponds. The existence of this counseling is very useful in providing knowledge so that people who work as Patin fish farmers can manage the time to replace pond water easily.
Pengolahan Sagu menjadi mie prebiotik sebagai makanan fungsional dan upaya peningkatan ketahanan pangan di Desa Alai Selatan, Kecamatan Tebing Tinggi Barat, Kepulauan Meranti Atria Martina; Wahyu Lestari; Tetty Marta Linda; Saberina Hasibuan; Imelda Wardani
Unri Conference Series: Community Engagement Vol 2 (2020): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.2.112-116

Abstract

Noodles are a processed food product favored by most Indonesians, generally made from wheat which must be imported. Kepulauan Meranti Regency is the largest producer of sago in Indonesia. One of the products made from sago that is widely produced in Meranti is sa.go noodle. Making noodles with local raw materials such as sago and sweet potato is an effort to improve food security. Traditional sago noodle has high carbohydrate and fiber content but still has disadvantages such as not having inulin content as a prebiotic, less antioxidant and a less attractive color, while purple sweet potato contains inulin as prebiotic components, high antioxidants, vitamins and minerals.The utilization of prebiotics insago noodle has multiple advantages since they improved probiotic growth in the body, sensory features and provide a more well-balanced nutritional composition as functional food. This community service program aims to training on the process of making prebiotic sago noodles to the community in Alai Selatan Village.This activities are carried out by course and practice methods.The results showed that the participants were interested in making prebiotic sago noodles because they had a more attractive features, more nutritious and had a delicious taste. Prebiotic sago noodles can be used as an effort to biodiversify sago noodle products to support food security and improve the community's economy.
Kebun Organik Di Masa Adaptasi Kebiasaan Baru Pandemi Covid-19 Dengan Memanfaatkan Sampah Rumah Tangga Rodesia Mustika Roza; Atria Martina; Hari Kapli; Bernadeta Leni Fibriarti; Wahyu Lestari
Unri Conference Series: Community Engagement Vol 3 (2021): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.3.658-664

Abstract

The World Health Organization has designated Covid-19 as a pandemic that causes activities outside the home to be restricted. In order to maintain health during the pandemic, many people are starting to choose foodstuffs produced from organic farming. The purpose of this activity is to provide information and motivation to society for reuse household waste with appropriate technology to produce compost. This service activity was carried out in Air Dingin Village, Bukit Raya District, Pekanbaru. The initial stage conveys material about the importance of maintaining health during the pandemic by consuming organic vegetables and fruits grown in their respective yards. Furthermore, the practice of making compost using the direct-use compost method and composting in a composter pot. Composting that reuses household waste has succeeded in opening up insight into the importance of the community's role in personal and environmental health and the public's understanding that a healthy life doesn't have to be expensive. In this activity, the participants showed their enthusiasm and stated that they would practice and share their knowledge with others in making compost to overcome the problem of household waste. This activity is also beneficial to keep the environment clean, healthy and beautiful.