Article Per Year (5 Year)
p-Index From 2020 - 2025
0.23
P-Index
This Author published in this journals
All Journal Jurnal Bios Logos Jurnal Kedokteran Brawijaya
Rooije R.H. Rumende
Program Doktor Ilmu Kedokteran, Minat Bio-Reproduksi, Pascasarjana Universitas Brawijaya Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
PENINGKATAN KUALITAS SPERMATOZOA PADA PROSES PEMISAHAN SPERMATOZOA DENGAN SENTRIFUGASI GRADIEN DENSITAS PERCOLL MELALUI PEMBERIAN FOSFOLIPID Rumende, Rooije R.H.; Kalim, Handono; Widodo, M Aris; Djati, Moch Sasmito
Jurnal Kedokteran Brawijaya Vol 23, No 2 (2007)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (947.856 KB) | DOI: 10.21776/ub.jkb.2007.023.02.3

Abstract

The separation of X and Y spermatozoa can be conducted by using Percoll Gradient Density Centrifugation(SGDP) method. It is, easy, valid, cheap and applicable method for developing made-insemination. Although it  has often been applied in sexing the spermatozoa, it was reported  that the method had often caused damages on the spermatozoa membranes and resulted in decreasing of the spermatozoa quality. The damages of spermatozoa after SGDP  process were specifically caused by (I) the loss of seminalplasma, (II) the increase of free radicals, and (III) the collision or friction among the spermatozoa. Among the three causal factors above, the third or the physical factor is claimed to be the most important one. It was assumed that the first and the second factors can be overcome if the third one is avoided. The spermatozoa membranes consist of lipids, protein, carbohydrate, and some substances at a low rate. Thegeneral objectives of this research was to identify the influence of the phospholipids PC (Phosphatidylcholin)to spermatozoa in order to avoid the damages of the spermatozoa in the process of SGDP.
Uji Efektivitas Kombinasi Ekstrak Etanol Jamur Kayu (Ganoderma lucidum) dengan Daun Sirsak (Annona muricata) terhadap Tikus Putih Galur WIstar (Rattus norvegicus) Hiperglikemia Naibaho, Jesica Christy; Rumende, Rooije R.H.; Pontororing, Hanny
JURNAL BIOS LOGOS Vol 10, No 1 (2020): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.10.1.2020.30001

Abstract

Uji Efektivitas Kombinasi Ekstrak Etanol Jamur Kayu (Ganoderma lucidum) dengan Daun Sirsak (Annona muricata) terhadap Tikus Putih Galur WIstar (Rattus norvegicus) Hiperglikemia(Effectivity Test of Wood Mushroom (Ganoderma lucidum) with Soursop Leaves (Annona muricata) Ethanol Extract Combination to Hyperglicemic Wistar Strain White Mouse (Rattus norvegicus) Jesica Christy Naibaho*, Rooije R.H. Rumende, Hanny PontororingProgram Studi Biologi FMIPA Universitas Sam RatulangiJl. Kampus Unsrat, Manado 95115*Email korespondensi: jesicachristynaibaho27@gmail.com, (Article History: Received 31-12-2019; Revised 15-01-2020; Accepted 24-01-2020) Abstrak Diabetes merupakan penyakit dengan indikasi pankreas tidak dapat memproduksi hormon insulin dalam jumlah cukup ataupun ketidakmampuan tubuh mempergunakan produksi insulin dengan baik. Penelitian ini bertujuan untuk menguji efektifitas dua konsentrasi ekstrak kombinasi jamur kayu (G. lucidum) dengan daun sirsak (A. muricata) dibandingkan dengan obat diabetes jenis glibenklamid.  Penelitian menggunakan metode eksperimental toksisitas akut dengan lima kelompok perlakuan dan lima ulangan. Analisis data yang digunakan adalah uji Friedman dengan uji lanjut Mann-Whitney. Hasil penelitian menunjukkan jumlah konsentrasi ideal kombinasi ekstrak jamur kayu (G. lucidum) dengan daun sirsak (A. muricata) yang dapat diinduksi terhadap tikus galur wistar hiperglikemia adalah 150 mg/kgBB dan 300 mg/kgBB tikus. Kombinasi ekstrak berpengaruh terhadap kontrol gula darah tikus galur wistar. Konsentrasi optimum kombinasi ekstrak di antara kedua dosis uji ekstrak adalah 300 mg/kgBB. Dosis 300 mg/kgBB kombinasi ekstrak dibandingkan dengan glibenklamid.Kata Kunci: Diabetes, pankreas, hormon insulin, glibenklamid   Abstract Diabetes is a pancreatic disease that can not produce enough insulin hormone or body’s inability to employ insulin hormones properly.The purpose of this study was to tested the effectiveness of two concentrations of wood mushrooms (G. lucidum) with soursop leaves (A. muricata) extract compared to glibenclamide as one of diabetes remedy. Research used acute toxicity experimental methods with five treatment groups and five repetitions. Data were analyzed using Friedman’s test and Mann-Whitney’s test. The results showed that the ideal number of wood mushrooms (G. lucidum) with soursop leaves (A. muricata) extract which can induced to wistar hyperglycemic mouse were 150 mg/kgBM and 300 mg/kgBM. Extract combination has an effect on mice glucose control. Optimum concentration of Extract Combination between the two extract test doses was 300 mg/kgBM. 300 mg/kgBM dose of wood mushrooms (G. lucidum) and soursop leaves (A. muricata)  extract avowed more effective than glibenclamide.Key words: Diabetes, pancreas, insulin hormone, glibenklamide
Co-Authors Diang, Yuri E.M. Edwin de Queljoe Gracia Alice Victoria Pollo Handono Kalim Hanny Pontororing M Aris Widodo Moch Sasmito Djati Mylton Mantouw, Mylton Naibaho, Jesica Christy Petta, Nabila S Roni Koneri Saroyo Saroyo Trina E. Tallei, Trina E.