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All Journal Jurnal Biodjati Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal BioEksakta : Jurnal Ilmiah Biologi Unsoed
Siti Samiyarsih
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Immunology & microbiology Veterinary

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Kajian Anatomi Daun dan Morfologi Nepenthes spp. Koleksi Kebun Raya Baturaden Kabupaten Banyumas Camelina Simbolon; Siti Samiyarsih; Wiwik Herawati
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 3 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.3.4560

Abstract

Pitcher plant (Nepenthes spp.) is one of the carnivorous plants which is classified as unique, because of the pitch on the Nepenthes spp. is a modified leaf tip that changes its shape and function to become a trap for insects or other small animals. The Baturraden Botanical Garden is the areas used for the conservation of various plant species both ex-situ and in-situ including Nepenthes spp. This aim of this study is to 1) determine the leaf anatomy and morphology characters of Nepenthes spp. which grows in the Baturraden Botanical Garden area, 2) determine the different characters of each species of Nepenthes spp. which grows in the Baturraden Botanical Garden area based on leaf anatomy and morphology. The research was conducted by direct observation, purposive sampling method, taking leaf samples of Nepenthes spp. in the greenhouse of the Baturaden Botanical Garden. Observation of leaf anatomical characters by embedding method with 1% safranin staining. The results of the measurement of anatomical and morphological characters were analyzed using ANOVA at the 95% and 99% confidence level, then continued with tests of Least Significant Difference (LSD) and the result observation from the morphological observations were analyzed descriptively. The results showed that the eight Nepenthes species had the same anatomical structure consisting of cuticle, epidermis, and mesophyll tissue with varying sizes between species. The morphological characters of eight Nepenthes species varied among species with different sizes. The petiole is only owned by N. ampullaria, N. mirabilis, N. rafflesiana, the pitch that has a waist shape is only owned by N. mirabilis, N, gracilis, and N. tobaica, the most striking pitch color is N. rafflesiana. The differences in the leaf anatomy characters of the eight Nepenthes species were found in the stomata width, cuticle thickness, epidermal thickness, mesophyll thickness, leaf length, leaf width, leaf stalk, tendril length, pitch diameter, pitch shape, pitch color
pemanfaatan ekstrak kulit buah manggis (Garcinia mangostana L.) sebagai pewarna alami jaringan daun dan batang krokot (Portulaca oleracea L.) Gita Fitri Yani; Muachiroh Abbas; Siti Samiyarsih
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (659.845 KB) | DOI: 10.20884/1.bioe.2020.2.2.2139

Abstract

The dye functions to clarify the plant tissue that will be observed with a microscope, the dye that is commonly used is synthetic dyes that are carcinogenic, the price is expensive and can pollute the environment. Natural dyes are an alternative to synthetic dyes because they are safe, inexpensive and environmentally friendly. natural dye sources obtained from plant parts such as fruit peels. Efforts to use mangosteen rind waste as an herbal remedy can also be used as a natural dye because it has a high enough anthocyanin content. Anthocyanins in mangosteen peel can be obtained through extraction. Maceration is an easy extraction method where the results are only affected by the type of solvent and the extraction time. Purslane (Portulaca oleracea L.) is used as research material because currently purslane has been widely used because of its nutritional content including high metabolic and antioxidant regualting substances. This study aims to determine the ability of mangosteen rind extract in coloring the leaf and stem tissue of purslane, and to determine the type of solvent and optimal immersion time to produce mangosteen rind extract so that it can color the leaf tissue and stems purslane well. The study was conducted experimentally using a Completely Randomized Design (CRD) with 2 factors: factor 1 was the type of solvent using ethanol 96% and citric acid 14%, the second factor was immersion time, namely 26, 27 and 28 hours. The data obtained were analyzed descriptively qualitatively including the contrast and clarity of the preserved tissue preparation of leaf and stem purslane. The results showed mangosteen rind extract has the ability to dye leaf tissue and stems. The type of 14% citric acid solvent with 28 hours soaking time was optimal in producing mangosteen rind extract so that it was able to dye the leaf and stem tissue of purslane.
Pollen Morphology Variation of Undergrowth Plants in Bantarbolang Nature Reserve, Pemalang, Central Java Anisatul Khabibah Zaen; Sukarsa Sukarsa; Siti Samiyarsih
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 1 No 2 (2019): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (401.998 KB) | DOI: 10.20884/1.bioe.2019.1.2.1812

Abstract

Undergrowth is ground cover plant which is generally in the form of herbs, grass or low shrubs. Mostly undergrowth is flowering plants. The Flower is part of plant for generative reproduction that consists of several organs that consist of pedicellus, receptacullum, perianthium, pistillum and stamen that contain pollen. Pollen is part of the flower that functions as a generative reproduction agent because it contains male gamete. Pollen has a distinctive character in each plant so that it can be used as a tool for identifying plants. Sampling location was conducted in Bantarbolang Nature Reserve, a conservation area located in Kebon Gede Village, Bantarbolang District, Pemalang Regency. The objective of research is to know the morphological character of undergrowth pollen in the Bantarbolang Nature Reserve. The research used a survey method with explorative sampling techniques. The variables that observed are the morphological character of pollen with the parameters consist of pollen unit, shape, aperture, diameter, and ornamentation. Sample preparation uses the acetolysis method and descriptive data analysis. Based on the results of the research found 6 species of undergrowth with different pollen morphological characters in the pollen shape (sub-speroidal and prolate), aperture (tricolpate, tricolporate and fenestrate) and ornamentation type (psilate, reticulate, perforate and echinate). The pollen units of all species have the same type, monad
Analisis Fenetik Kultivar Krisan (Chrysanthemum morifolium Ramat.) Indah Anugrahsari; Sukarsa Sukarsa; Siti Samiyarsih
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 2 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2016.33.1.325

Abstract

Chrysanth (Chrysanthemum morifolium Ramat.) atau dikenal sebagai Seruni merupakan tanaman herba hias. Chrysanth merupakan salah satu komoditas tanaman hias utama yang memiliki keragaman kultivar, seperti dapat dilihat dari bentuk dan variasi warna bunganya. Penelitian ini bertujuan untuk menyelidiki keragaman dan hubungan kesamaan kultivar chrysanth. Metode penelitian yang digunakan purposive sampling. Data dari chrysanth kultivar morfologi dan anatomi daun karakter dianalisis berdasarkan hubungan kemiripan dengan UPGMA (Unweighted Pasangan Metode Group dengan Arithmetic mean) metode menggunakan 5.05 software MEGA. Berdasarkan penelitian, delapan kultivar chrysanth diperoleh, yaitu C. morifolium 'Cut Nyak Dien', C. morifolium 'Dewi Ratih', C. morifolium 'Dwina Kencana', C. morifolium 'Dwina Pelangi', C. morifolium 'Elora ', C. morifolium' Pasopati ', C. morifolium' Puspita Nusantara ', dan C. morifolium' Swarna Kencana '. Kesamaan terdekat adalah antara C. morifolium 'Dwina Kencana' dan C. morifolium 'Dwina Pelangi' kultivar, dengan indeks ketidaksamaan terkecil 0,152, sedangkan kesamaan paling jauh adalah antara C. morifolium 'Elora' dan C. morifolium 'Swarna Kencana 'kultivar, dengan indeks ketidaksamaan terbesar 2,256.
Respon beberapa Varietas Padi terhadap Stress Garam Sugiyono Sugiyono; Siti Samiyarsih
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 22, No 2 (2005)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2005.22.2.93

Abstract

A study on the Responses of Several Rice Varieties toward Salt Stress has been carried out with a view to: 1) study the effect of salt stress on the physiological and anatomical responses of several rice varieties; 2) determine salt tolerant rice variety(ies). Research results showed that there were both physiological and anatomical changes on salt-stressed rice.  Those changes were determined by the level of salt concentration given.  The higher the concentration the more obvious the changes appeared.  There was a dramatic decrease in the total chlorophyll content of the salt-stressed leaf, coupled by a sharp decrease in the chlorophyll a/b ratio. Salt stress also resulted in the decrease in mesophyll thickness, and in turn leads to the decrease of leaf’s length and width.  In general, salt stress resulted in the decrease of plant growth, as indicated by the decrease of plant’s fresh and dry weights, eventual plant’s death was therefore imminent. The rice varieties used were sensitive to salt stress since an exposure of 50 mM NaCl has already resulted in the interference of rice growth.
Thidiazuron Improved Aglaonema ‘Ruby’ Microshoot Multiplication for Mass Production and Microfloriculture Development Allya Alifia Purbaya; Rendie Prasetyo; Siti Samiyarsih; Sugiyono
Jurnal Biodjati Vol 10 No 1 (2025): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i1.34401

Abstract

Aglaonema 'Ruby' is a hybrid Aglaonema with a dominant green leaf pattern and a red accent in the middle. This cultivar is widely cultivated and in great demand, and it can potentially be used in micro floriculture development. Conventional Aglaonema propagation through stem cuttings can only produce 1-3 shoots. Therefore, the in vitro culture method is proposed. The objectives of this study were to evaluate the effect of three synthetic cytokinins (BAP, Kinetin, and TDZ—the latter being a phenylurea derivative with cytokinin-like activity) and concentrations on the multiplication of Aglaonema 'Ruby' microshoot. The research has been carried out experimentally using a split-plot design. The main plot was cytokinin types, consisting of BAP, Kinetin, and Thidiazuron; the subplot was cytokinin concentrations at 0, 5, 10, 15, and 20 µM. The measured parameters include shoot emergence time, number of shoots, leaves, and shoot length. The data were analyzed using an analysis of variance (ANOVA) followed by Duncan's multiple range test at 95% confidence level. It can be concluded that the growth of Aglaonema 'Ruby' micro shoots was controlled by the type and concentration of cytokinin given. Thidiazuron was better than Kinetin and BAP in stimulating the growth of Aglaonema 'Ruby' microshoots. Cytokinin at 10 µM seemed to be effective in improving Aglaonema 'Ruby' micro shoots multiplication. Thidiazuron at 10 µM can increase the production of Aglaonema 'Ruby' shoot to support both mass production of seedlings and microfloriculture products. Further studies are needed to optimize shoot and root development to produce good plantlets, easing the subsequent acclimatization.
Co-Authors Allya Alifia Purbaya Anisatul Khabibah Zaen Camelina Simbolon Gita Fitri Yani Indah Anugrahsari Jayawarsa, A.A. Ketut Muachiroh Abbas Rendie Prasetyo Sugiyono Sugiyono Sugiyono Sukarsa Sukarsa wiwik herawati