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Imron Imron
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MHC-II MARKER POTENTIAL LINKED TO MOTILE AEROMONAD SEPTICAEMIA DISEASE RESISTANCE IN AFRICAN CATFISH (Clarias gariepinus) Rommy Suprapto; Alimuddin Alimudddin; Sri Nuryati; Imron Imron; Huria Marnis; Bambang Iswanto
Indonesian Aquaculture Journal Vol 12, No 1 (2017): (June 2017)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (497.4 KB) | DOI: 10.15578/iaj.12.1.2017.21-28

Abstract

One of the important issues in catfish farming is motile aeromonad septicaemia (MAS) disease caused by the bacterium Aeromonas hydrophila. This study aimed to find the MHC-II marker potential for marker-based selection to generate MAS disease resistance of African catfish. PCR method was applied to identify catfish (body length: 7-8 cm) population that have MHC-II marker. Fish with and without the marker were then challenged by intraperitonially injecting of 0.1 mL/fish with A. hydrophila (105 cfu/mL). The results showed that the survival of fish having MHC-II marker (77.50 ± 4.00%) was higher than that of fish without the marker (53.33 ± 4.77%). Fish carrying MHC-II marker fish has also higher total erythrocytes, total leukocytes, phagocytic activity, and hematocrit levels than that of fish without the marker. The PCR results using specific primer for MHC-II showed a specific DNA band of 426 bp in fish having the marker, while there were no DNA bands in fish without the marker. Results of the PCR analyses showed that the percentage of progenies carrying MHC-II marker was 80%, while progenies from broodstock without the marker was 0%; this indicated that MHC-II marker could be inherited to the offsprings. Thus, the MHC-II marker could be used as a molecular marker of MAS disease resistance catfish.
IDENTIFICATION OF SNP SPESIFIC MARKER FOR CRUSTACEAN HYPERGLYCEMIC HORMONE GENE: A SOMATIC GROWTH-RELATED IN GIANT FRESHWATER PRAWN (Macrobrachium rosenbergii) Asep Sopian; Alimuddin Alimuddin; Imron Imron; Harry Krettiawan; Fajar Anggraeni; Desy Nurul Astuti
Indonesian Aquaculture Journal Vol 12, No 1 (2017): (June 2017)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (336.842 KB) | DOI: 10.15578/iaj.12.1.2017.7-13

Abstract

High size variation of giant freshwater prawn was found in harvest and resulting in low productivity. Marker assisted selection may be useful to generate broodstock that produces progeny with high growth and homogeneity. This study was conducted to obtain growth related molecular marker in giant freshwater prawn. Genomic DNA was extracted from swimming leg (pleiopods) of 10 giant freshwater prawns fifth Generation for existence of SNP identification, consisted of 5 fast growth (FG) and 5 slow growth (SG).  While for SNP confirmation and resolving power of specific primer studies. The pleiopods sample was taken from six generation of 201 giant freshwater prawns, consisted of 129 fast-growth (FG) with 16.06 ± 2.48 g body weight and 72 slow-growth (SG) with 6.05 ± 0.90 g body weight. Oligonucleotide primers were designed according to Gene Bank database of crustacean hyperglycemic hormone (CHH) gene sequence. The amplified DNA fragment was then sequenced. The results of sequencing showed there was one base different in nucleotides of FG and SG prawns. Six set of primers were designed based on those CH gene sequence. PCR analysis resulted one set of primers which showed a specific amplification product of 280 bp for growth. The result of sequence analysis using the basic local alignment search tools showed that the nucleotide sequence of those PCR products had similarity of 99%-100% with CHH gen of M. rosenbergii. Thus, a candidate of growth related molecular marker have been identified for giant freshwater prawn.
PERFORMA REPRODUKSI DAN PERTUMBUHAN UDANG GALAH BETINA Macrobrachium rosenbergii SETELAH PEMBERIAN HORMON MEDROXY PROGESTERON ACETAT MELALUI PAKAN Fajar Anggraeni; Fajar Anggraeni; Desak Made Malini; Imron Imron
Jurnal Riset Akuakultur Vol 16, No 2 (2021): (Juni, 2021)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (97.38 KB) | DOI: 10.15578/jra.16.2.2021.83-91

Abstract

Salah satu permasalahan pada budidaya udang galah adalah keberadaan udang galah betina bertelur pada saat pembesaran. Penghambatan pema tangan gonad menggunakan hormon medroxyprogesterone acetat (MPA) yang diberikan secara injeksi pada udang galah dapat menekan indeks kematangan gonad (IKG), perkembangan gonad, dan meningkatkan laju pertumbuhan. Namun, pemberian MPA secara injeksi dapat meningkatkan stres dan kematian pada udang galah, Tujuan dari penelitian ini adalah untuk mengkaji pengaruh pemberian hormon medroxy progesteron acetat melalui pakan terhadap performa reproduksi dan pertumbuhan. Metode yang digunakan adalah metode eksperimental, desain penelitian menggunakan rancangan acak lengkap (RAL) dengan empat perlakuan dan lima kali pengulangan. Perlakuan yang diberikan adalah kontrol (hormon MPA pada konsentrasi 0 mg/kg pakan), P-1 (hormon MPA pada konsentrasi 50 mg/kg pakan), P-2 (hormon MPA pada konsentrasi 100 mg/kg pakan), dan P-3 (hormon MPA pada konsentrasi 150 mg/kg pakan) yang diberikan pada udang galah betina selama 60 hari. Parameter yang diamati adalah kematangan gonad, egg clutch somatic index (ESI), fekunditas, kadar estradiol, laju pertumbuhan spesifik, dan sintasan. Hasil penelitian menunjukkan bahwa pemberian hormon MPA dengan konsentrasi 50, 100 dan 150 mg/kg pakan secara signifikan menghambat reproduksi dan pada konsentrasi 100 mg/kg pakan dapat meningkatkan laju pertumbuhan pada udang galah betina umur lima bulan. ESI, fekunditas dan tingkat sintasan tidak terpengaruh oleh perlakuan. Kesimpulan dari penelitian ini adalah pemberian hormon medroxy progesteron acetat melalui pakan pada konsentrasi 100 mg/kg pakan secara signifikan dapat menghambat pematangan gonad dan peningkatan pertumbuhan udang galah betina.One of the problems in giant freshwater prawn farming is female prawns laying eggs during grow-out, leading to reduced somatic growth of the prawns. Inhibition of gonad maturation using medroxyprogesterone acetate (MPA) hormone via injection in giant freshwater prawns can suppress the gonad maturity index (GSI), gonad development, and increase the growth rate. However, the administration of MPA by injection could increase stress and mortality in giant freshwater prawns. The purpose of this study was to examine the application of MPA through dietary administration on the reproductive performance and growth of female giant freshwater prawns. The experiment was arranged in a completely randomized design with four treatments and five replications. Treatments of MPA at concentrations of 0 mg/kg feed (as control), 50 mg/kg feed (P-1), 100 mg/kg feed (P-2), and 150 mg/kg feed (P-3) were supplemented in a commercial artificial feed and fed to the freshwater prawns for 60 days. Parameters of gonad maturation, egg clutch somatic index (ESI), fecundity, oestradiol concentration, specific growth rate, and survival rate were measured during the research period. The result showed that MPA administration at concentrations of 50, 100, and 150 mg/kg feed significantly inhibited gonad maturation, and at concentration 100 mg/kg feed increased growth of female giant freshwater prawn at five-month age. The ESI, fecundity and survival, however, were not affected by the treatment. This study concludes that the administration of medroxyprogesterone acetate hormone through feed significantly inhibited gonad maturation and increasedthe growth of female giant freshwater prawns.
HIBRIDISASI INTRASPESIFIK ANTAR DUA POPULASI IKAN GURAMI GALUNGGUNG (Osphronemus goramy, Lacepede, 1801) Otong Zenal Arifin; Imron Imron; Asependi Asependi; Ade Hendri; Nandang Muslim; Akhmad Yani
Jurnal Riset Akuakultur Vol 12, No 4 (2017): (Desember 2017)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (287.198 KB) | DOI: 10.15578/jra.12.4.2017.315-323

Abstract

Ikan gurami (Osphronemus goramy) populasi Galunggung Hitam (GH) dan Galunggung Putih (GP) telah dibudidayakan secara luas di wilayah Priangan. Dalam upaya mendapatkan strain unggul ikan gurami untuk budidaya, hibridisasi intraspesifik, yaitu persilangan antar strain berbeda dalam spesies yang sama, merupakan pendekatan sederhana yang menarik untuk dieksplorasi. Penelitian ini bertujuan untuk mendapatkan informasi mengenai keragaan hasil pemijahan dua populasi ikan gurami. Penelitian dilaksanakan di Balai Pengembangan Budidaya Ikan Gurami dan Nilem, Singaparna. Empat populasi ikan diperoleh dari hasil pemijahan dua arah antara ikan gurami yang berwarna putih dan berwarna hitam. Hasil yang diperoleh menunjukkan, hibridisasi antara induk betina gurami Galunggung Hitam dengan induk jantan Galunggung Putih (GH><GP), menghasilkan rerata panjang standar akhir 17,76 ± 1,129 cm; laju pertumbuhan mutlak 10,98 ± 1,240 cm; dan laju pertumbuhan spesifik 0,32 ± 0,017% per hari. Pada karakter lebar akhir, ikan gurami hibrida memiliki nilai 8,98 ± 0,485 cm; pertumbuhan mutlak lebar 5,68 ± 1,014 cm; dan laju pertumbuhan spesifik 0,33 ± 0,030% per hari. Bobot akhir ikan gurami hibrida sebesar 301,9 ± 6,63 g; laju pertumbuhan mutlak 295,6 ± 17,42 g; laju pertumbuhan spesifik 1,29 ± 0,017% per hari; dan sintasan sebesar 77,6 ± 5,26%; serta produktivitas sebesar 8,8 ± 0,70 kg/m2. Nilai heterosis ikan gurami hibrida (GH><GP) untuk seluruh karakter adalah positif. Nilai heterosis karakter bobot akhir adalah 14,1%; pertumbuhan mutlak 14,6%; laju pertumbuhan spesifik 5,38%; sintasan 31,75%; dan produktivitas sebesar 51,72%.The black Galunggung and white Galunggung strains of the giant gourami (Osphronemus goramy) have been widely cultured in Priangan areas, Indonesia. Intraspecific hybridization, a crossing between different strains of the same species, is a simple and commonly used approach to produce a superior strain for aquaculture purposes. This study was aimed to obtain information on the performance of gourami spawned from two different populations. The experiment was conducted at the Aquaculture Development Center of Giant Gourami and Nilem Carp, Singaparna. Four populations of giant gourami were obtained from spawning between white and black giant gourami. The results obtained showed that the hybrid resulted from the female of Black Galunggung and male of White Galunggung (GH><GP) produced the final average standard length of 17.76 ± 1.129 cm, standard length gain of 10.98 ± 1.240 cm, and specific growth rate in standard length of 0.32 ± 0.017% per day. On height character, the hybrid gourami had the final height of 8.98 ± 0.485 cm, a height gain of 5.68 ± 1.014 cm, and specific growth rate in height of 0.33 ± 0.030% per day. The final weight of the hybrid gourami of 301.9 ± 6.63 g, weight gain of 295.6 ± 17.42 g, the specific growth rate in weight of 1.29 ± 0.017% per day, and survival rate of 77.6 ± 5.26%, as well as the productivity of 8.8 ± 0.70 kg/m2. Heterosis value of the hybrid gourami (GH><GP) for the whole character was positive, with the heterosis value on the final weight of 14.1%, growth of 14.6%, the specific growth rate of 5.38%, the survival rate of 31.75%, and productivity of 51.72%.
Co-Authors Ade Hendri Akhmad Yani Alimuddin Alimuddin Alimudddin Asep Sopian Asependi Asependi Bambang Iswanto Desak Made Malini Desy Nurul Astuti Fajar Anggraeni Fajar Anggraeni Hary Krettiawan Huria Marnis Nandang Muslim Otong Zenal Arifin Rommy Suprapto Sri Nuryati