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Desy Sugiani
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PENINGKATAN KEKEBALAN SPESIFIK ANTI STREPTOCOCCUS PADA BUDI DAYA IKAN NILA Hambali Supriyadi; Desy Sugiani; Uni Purwaningsih
Jurnal Riset Akuakultur Vol 2, No 1 (2007): (April 2007)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (128.923 KB) | DOI: 10.15578/jra.2.1.2007.87-92

Abstract

Uji lapang vaksin Streptococcus telah dilakukan dengan tujuan untuk mengetahui efektivitas vaksin dan respon kebal pada ikan nila (Oreochromis niloticus) terhadap rangsangan yang diberikan. Penelitian dilakukan pada keramba jaring apung (KJA). Vaksin yang digunakan adalah vaksin S1N8 dan GM2.4 berupa vaksin yang diinaktivasi dengan formalin 0,3%. Aplikasi vaksin dilakukan secara bertahap yaitu vaksin awal (priming) diberikan melalui rendaman, sedangkan vaksin ulang diberikan melalui suntikan. Dosis vaksin awal yaitu 10 mL vaksin/100 L air untuk 1.000 ekor ikan direndam selama 15 menit, sedangkan booster diberikan melalui suntikan 0,2 mL/ ekor ikan. Hasil menunjukkan bahwa kombinasi pemberian vaksin priming melalui rendaman dan booster dengan suntikan untuk vaksin S1N8 menghasilkan sintasan paling tinggi (70,3%—72,5%), apabila dibandingkan dengan vaksin GM2.4 (59,3%— 62,5%) dan kontrol (35,5%—42,0%).Field study of vaccines S1N8 and GM2.4 with the aims to evaluate the effectiveness of vaccine and the immune response of nile tilapia ( Oreochromis niloticus ) against the vaccines. The research have been conducted in floating net cage. Vaccine tested were produced from Streptococcus iniae isolates S1N8 and GM2.4 which was prepared by formalin killed of 0.3%(v/v). Vaccine delivery were given in two steps i.e. priming with immersion, and booster through injection. The dose of vaccine for priming was 10 mL of vaccine/100 L water immersed for 1,000 fish for 15 minutes. Booster were delivered by injection as much as 0.2 mL/fish. The results indicated that combination of vaccine delivering of immersion (priming) and injection (booster) especially for S1N8 vaccine were the highest percent of survival rate (70.3%—72.5%) as compared with GM2.4 vaccine (59.3%—62.5%) and control (35.5%—42.0%).
EFEKTIVITAS ANTIBIOTIKA DAN VAKSIN DALAM PENANGGULANGAN PENYAKIT STREPTOCOCCOSIS PADA IKAN NILA (Oreochromis niloticus) Hambali Supriyadi; Taukhid Taukhid; Ani Widiyati; Desy Sugiani
Jurnal Riset Akuakultur Vol 1, No 1 (2006): (April 2006)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (348.683 KB) | DOI: 10.15578/jra.1.1.2006.61-65

Abstract

Penelitian dengan tujuan untuk mengetahui antibiotik yang efektif untuk pengobatan penyakit streptococcosis, serta mendapatkan cara pencegahan penyakit secara biologis yaitu melalui penggunaan vaksin telah dilakukan di Laboratorium Riset kesehatan Ikan Pasar Minggu. Tiga jenis antibiotika yaitu Neomycin, Oxytetracyclin, dan Enrofloxacin diuji efektivitasnya terhadap 4 isolat bakteri Streptococcus iniae yaitu Y2N7, Y2N9, GM2.4, dan S1N8 melalui uji zona hambatan dan konsentrasi hambat minimum (MIC). Uji imunogenitas diuji dengan cara pembuatan vaksin dari isolat yang digunakan yang kemudian dievaluasi level titer antibodi yang diproduksi. Hasil penelitian menunjukkan bahwa enrofloxacin merupakan antibiotik yang efektif terhadap semua isolat yang diuji, sedangkan neomycin efektif hanya untuk isolat Y2N7. Isolat GM2.4 relatif memiliki sifat immunogenitas lebih baik dibanding dengan isolat uji lainnya.Research with the aims to evaluate the effectiveness of several antibiotics against 4 (four) streptococcus iniae isolates, and evaluation of immunogecity of those isolate to be used for disease control (vaccine) have been conducted at Fish Health Research Laboratory Pasar Minggu. The effectiveness of three antibiotics namely Neomycin, Oxytetracyclin, and Enrofloxacin have been tested against 4 (four) isolates Y2N7, Y2N9, GM2.4, and S1N8. The immunogenicity of those isolates were also tested by developing vaccine and evaluated through the production of antibody titer level. The results indicated that enrofloxacin was effective against all isolates tested, meanwhile neomycin only effective against isolate Y2N7. Isolate of GM2.4 was relatively immunogenic as compared to the other isolates.
Co-Authors Ani Widiyati Hambali Supriyadi Hambali Supriyadi Taukhid Taukhid Uni Purwaningsih