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All Journal Collaborative Medical Journal (CMJ) JURNAL KESEHATAN PERINTIS JURNAL ANALIS LABORATORIUM MEDIK Jurnal Sains dan Teknologi Laboratorium Medik Jurnal Pengabdian kepada Masyarakat
Hartini H
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Journal : JURNAL ANALIS LABORATORIUM MEDIK

 1 
EVALUATION OF PLATELET RICH PLASMA (PRP) PREPARATION PROCEDURE Yuliandari, Aisyara; Damhuri, Prima Octafia; Margaretta, Tiur Sherly; Priskilla, Sarah Ester; Hartini H
Jurnal Analis Laboratorium Medik Vol 8 No 2 (2023): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v8i2.4509

Abstract

The success of PRP therapy in repairing tissue damage is influenced by the PRP preparation procedure. Currently, there’s no standardization of PRP preparation procedures, and various techniques are used, such as the use of anticoagulants and different centrifugation speeds. This study aimed to evaluating the PRP preparation procedures based on the centrifugations steps (single and double centrifugation) and the use of  anticoagulants variation (sodium citrate, EDTA and ACD-A). This study was an experimental study and used blood samples from respondents. The selected respondents must meet inclusion and exclusion criteria. The treatment groups in this study were the single centrifugation group and the double centrifugation group. Each group will be divided into 3 subgroups with different anticoagulant usage (sodium citrate, EDTA and ACD-A). Statistical analysis results showed a significant difference in the mean platelet count in the sodium citrate , EDTA, ACD-A groups with single and double centrifugation steps. Evaluation of platelet preparation procedures in this study, a higher platelet count was obtained, specifically in the sodium citrate group (494 x 103 cells/µL), EDTA group (829.4 x 103 cells/µL), and ACD-A group (607.1 x 103 cells/µL), compared to single centrifugation in the sodium citrate group (354.8 x 103 cells/µL), EDTA group (408.1 x 103 cells/µL), and ACD-A group (390.6 x 103 cells/µL). The highest platelet count in PRP was achieved with the preparation procedure using EDTA as the anticoagulant with double centrifugation. Further research is necessary to evaluate PRP preparation procedures regarding the concentration of growth factors present in PRP.
The Effectiveness of PRP on Reducing Blood Glucose Levels in Diabetic Mice Yuliandari, Aisyara; Hartuti, Yeli; Tomahu, Dea Yuni Putri; Hartini H
Jurnal Analis Laboratorium Medik Vol 7 No 2 (2022): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v7i2.3386

Abstract

PRP contains growth factors that have the potential to repair tissue damage, such as pancreatic damage in diabetes mellitus. Pancreas damage in diabetes mellitus is characterized by hyperglycemia. This study aimed to determine the effectiveness of PRP in reducing blood glucose levels in diabetic mice. Male Swiss Webster mice divided into 5 groups (normal group, DM group, PRP1 group, PRP2 group and PRP3 group). The DM, PRP1, PRP2 and PRP3 groups were given streptozotocin 45 mg/kgBW to induce diabetes mellitus. Mice in the PRP1, PRP2, and PRP3 groups that have indicated diabetes mellitus with glucose levels > 200 mg/dL will be given PRP with a dose of 0.1 mL; 0.3 mL; 0.5 mL intraperitoneally for 4 weeks. Data on blood glucose levels were analyzed using one-way ANOVA test and LSD test. The use of PRP in various doses, namely 0.1 mL, 0.3 mL, 0.5 mL, can reduce blood glucose levels in the PRP1 group (195.8 ± 14.04 mg/dl), PRP2 group(176.6 ± 9.37 mg/dl), PRP3 group (121 ± 5.52 mg/dl) compared to the DM group (392.6 ± 18.09 mg/dl). Dose of PRP of 0.5 mL gave a better effect on pancreatic tissue repair than the PRP1 and PRP2 groups, which were characterized by glucose levels that were close to the normal group (106.8 ± 10.61 mg/dl). Based on the results of the study, PRP 0.5 mL is effective in repairing pancreatic tissue which is characterized by a decrease in blood glucose levels in diabetic mice.
Co-Authors Agnes Farianti Rezeki Sihombing Aisyara Yuliandari Alfredo Triseptian Silalahi Cristina Sigiro Damhuri, Prima Octafia Dian Ayu Hartuti, Yeli Karolina Rosmiati Margaretta, Tiur Sherly Priskilla, Sarah Ester Puspita Daeli Tomahu, Dea Yuni Putri Yuliandari, Aisyara