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EVALUATION OF PLATELET RICH PLASMA (PRP) PREPARATION PROCEDURE Yuliandari, Aisyara; Damhuri, Prima Octafia; Margaretta, Tiur Sherly; Priskilla, Sarah Ester; Hartini H
Jurnal Analis Laboratorium Medik Vol 8 No 2 (2023): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v8i2.4509

Abstract

The success of PRP therapy in repairing tissue damage is influenced by the PRP preparation procedure. Currently, there’s no standardization of PRP preparation procedures, and various techniques are used, such as the use of anticoagulants and different centrifugation speeds. This study aimed to evaluating the PRP preparation procedures based on the centrifugations steps (single and double centrifugation) and the use of  anticoagulants variation (sodium citrate, EDTA and ACD-A). This study was an experimental study and used blood samples from respondents. The selected respondents must meet inclusion and exclusion criteria. The treatment groups in this study were the single centrifugation group and the double centrifugation group. Each group will be divided into 3 subgroups with different anticoagulant usage (sodium citrate, EDTA and ACD-A). Statistical analysis results showed a significant difference in the mean platelet count in the sodium citrate , EDTA, ACD-A groups with single and double centrifugation steps. Evaluation of platelet preparation procedures in this study, a higher platelet count was obtained, specifically in the sodium citrate group (494 x 103 cells/µL), EDTA group (829.4 x 103 cells/µL), and ACD-A group (607.1 x 103 cells/µL), compared to single centrifugation in the sodium citrate group (354.8 x 103 cells/µL), EDTA group (408.1 x 103 cells/µL), and ACD-A group (390.6 x 103 cells/µL). The highest platelet count in PRP was achieved with the preparation procedure using EDTA as the anticoagulant with double centrifugation. Further research is necessary to evaluate PRP preparation procedures regarding the concentration of growth factors present in PRP.
UJI EFEKTIFITAS LARVASIDA EKSTRAK DAUN TANAMAN CIPLUKAN (PHYSALIS ANGULATA L.) TERHADAP LARVA AEDES AEGYPTI Mulyani, Weni; Damhuri, Prima Octafia; Wahyu, Nur Azlindasri
Jurnal Analis Laboratorium Medik Vol 9 No 2 (2024): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v9i2.5349

Abstract

Demam Berdarah Dengue (DBD) merupakan penyakit yang disebabkan oleh virus dengue yang ditularkan melalui gigitan nyamuk Aedes aegypti. Penggunaan insektisida alami yang berasal dari tanaman merupakan alternatif dari pengendalian vektor yang aman bagi lingkungan. Salah satu insektisida alami ialah tanaman yang kaya akan metabolit sekunder, seperti daun ciplukan (Physalis angulata L.) . Tujuan penelitian ini adalah untuk mengetahui efektifitas ekstrak daun tanaman ciplukan (Physalis angulata L.) terhadap larva Aedes aygypti. Jenis penelitian ini adalah eksperimental dengan rancangan penelitian post test only group design. Penelitian ini terdiri dari 6 kelompok uji yaitu ekstrak daun tanaman ciplukan (Physalis angulata L.) konsentrasi 1%, 2%, 3% , kontrol positif abate, kontrol positif alkohol, dan kontrol negatif. Pengamatan dilakukan pada waktu 30 menit, 60 menit, 90 menit, 120 menit, 150 menit dan 180 menit. Dari hasil uji deskriptif, terlihat bahwa rata-rata jumlah larva Aedes aegypti yang mati meningkat seiring dengan peningkatan konsentrasi ekstrak daun ciplukan (Physalis angulata L.) dan lamanya waktu pengamatan. Pada konsentrasi 1%, rata-rata larva yang mati adalah 0,67 dengan standar deviasi 0,816, menunjukkan  bahwa sebagian besar larva masih bertahan hidup pada konsentrasi rendah. Konsentrasi 2 % menunjukkan sedikit peningkatan kematian dengan rata-rata 1,00 larva yang mati dan variabilitas lebih  tinggi dengan standar deviasi 1,265. Konsentrasi 3 % memberikan hasil yang lebih signifikan dengan rata-rata kematian 3,00 larva dan standar deviasi 2,828, hal ini menunjukkan terdapat efek yang lebih kuat pada konsentrasi yang lebih tinggi. Dapat disimpulkan bahwa ekstrak daun tanaman ciplukan (Physalis angulata L.) memiliki efektifitas terhadap larva Aedes aegypti.
Penyuluhan Kesehatan Bahaya HIV dan AIDS Dan Pencegahannya di SMA Santa Maria Pekanbaru Damhuri, Prima Octafia
AMMA : Jurnal Pengabdian Masyarakat Vol. 1 No. 06 (2022): AMMA : Jurnal Pengabdian Masyarakat
Publisher : CV. Multi Kreasi Media

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

HIV and AIDS is one of the dangerous diseases that cannot be cured. Based on data from the Riau Health Office, the number of HIV cases in 2019 was 533 cases. The number of HIV cases in the 15-19- year-old was 7 cases, the 20-24-year-old was 77 cases and the highest was found in the 25-49-year-old was 397 cases. This was the basis of the author's interest in conducting counseling about HIV and AIDS at Santa Maria High School Pekanbaru. This counseling aims to provide information and understanding about HIV and AIDS. The method used was counseling by delivering information through lectures and conducting questions and answers to the community to increase understanding and awareness about HIV and AIDS. This counseling would be held on April 5, 2022 at 09.00 WIB to 11.00 WIB at Santa Maria High School Pekanbaru. The result of this counseling was the implementation of counseling about HIV and AIDS to Santa Maria High School Pekanbaru students by providing information and understanding about the importance of a healthy lifestyle to prevent people from contracting HIV and AIDS.