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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Al Ishlah Jurnal Pendidikan Jurnal Penelitian Pendidikan IPA (JPPIPA)
Agung Pramana Warih Marhendra
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Universitas Brawijaya, Malang 65145, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Physics Other

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Effect of Acute Toxicity of Commercial Organophosphate Insecticide Based on Chlorpyrifos on Fejervarya limnocharis Tadpoles (Anura: Dicroglossidae): Acute Toxicity of Commercial Chloropyrifos on Fejervarya limnocharis Tadpole Shima Ramadani; Agung Pramana Warih Marhendra; Dewa Gede Raka Wiadnya; Nia Kurniawan
Journal of Tropical Life Science Vol. 12 No. 2 (2022)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.12.02.09

Abstract

Organophosphate pesticides, especially chlorpyrifos, are one of the most widely used insecticides in agriculture, but their toxicity and potential sensitivity effects on Anura, especially Fejervarya limnocharis are still unknown. The purpose of this investigation is to study F. limnocharis (Anura: Dicroglossidae) tadpole sensitivity to lethal (survivability) and sublethal effects (morphological alterations and swimming activity) of chlorpyrifos in Dursban 200EC commercial formula under acute exposure. An acute toxicity test was carried out on ten tadpoles (Gosner 25) in each repetition. The sample was obtained from artificial reproduction by injecting the Trial Batch 2000 IU hCG by Kings Lab. The acute toxicity testing consisted of three replicates with a chlorpyrifos concentration of 0; 0.4; 0.8; 1; 2; 4; and 8 µg/L. Physico-chemical parameters, mortality, morphological, and swimming alterations of each concentration were observed at 24th, 48th, 72nd, and 96th hours. The LC50 of chlorpyrifos for F. limnocharis tadpoles was 2.86 µg/L. The percentage of survivability F. limnocharis tadpoles decreased after exposure to chlorpyrifos above 1µg/L, while morphological alterations were observed in 2 µg/L and 4 µg/L after 48th hours exposure, and the swimming alterations have occurred at 24th hours in 1; 2; 4 and 8 µg/L. Morphological alterations were observed including asymmetrical body shape, edema, and abnormal tail shape. Based on the LC50 value, commercial chlorpyrifos has high-level toxicity on F. limnocharis tadpoles.Keywords: Acute, Chlorpyrifos, Dursban 200 EC, Fejervarya limnocharis, Tadpoles
Revisi Peta Distribusi Ikan Padi (Adrianichthyidae: Oryzias spp.) Berdasarkan Identifikasi Morfologi di Paparan Sunda Indonesia Akhsan Fikrillah Paricahya; Kiki Nur Azam Kholil; Ifa Sufaichusan; Muh. Herjayanto; Rengga Retno Laila Saputri; Abd. Rahem Faqih; Agung Pramana Warih Marhendra
Jurnal Penelitian Pendidikan IPA Vol 10 No 5 (2024): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i5.6758

Abstract

Members of the Adrianichthyidae family share typical distributions, as does the javanicus group, which inhabits western parts of Southeast Asia. Javanicus group members, specifically Oryzias javanicus and Oryzias hubbsi, is the only Adrianichthyidae species that can be spotted in Sundaland Indonesia. Morphological identification and the species distribution mapping of Oryzias specimens from Sundaland in this study were interpreted using ecoregional and dendogram tree approach. Specimens were collected from 11 areas representing five ecoregions in Sundaland Indonesia. Specimens were collected from islands of Java, Sumatra, Belitung and Kalimantan. Six individuals from each population of Oryzias were sampled for morphological identification. Specimens collected from estuaries were morphologically identified as O. javanicus and specimens from freshwater identified as O. hubbsi. Distribution of O. hubbsi from Salatiga is the first record from Central East Java (CEJ) ecoregion, which was previously only distributed in Southern Sumatra-Western Java (SWJ) ecoregion. Morphology of O. hubbsi from Sundaland Indonesia has mean of Standard Length (SL) 1.56 cm, Total Length (TL) 1.94 cm, Anal rays (A) 16, Pectoral rays (P) 8, and Dorsal rays (D) 6. Meanwhile, morphology of O. javanicus from Sundaland has mean of SL 2.07 cm, TL 2.56 cm, A 22, P 11, and D 6.
Molecular Analysis Using Cytochrome Oxidase Subunit I (COI) to Confirming New Recorded Oryzias hubbsi in Central East Java Ecoregion Akhsan Fikrillah Paricahya; Kiki Nur Azam Kholil; Rengga Retno Laila Saputri; Muh. Herjayanto; Dewa Gede Raka Wiadnya; Abd. Rahem Faqih; Agung Pramana Warih Marhendra
Jurnal Penelitian Pendidikan IPA Vol 10 No 7 (2024): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i7.6802

Abstract

Oryzias hubbsi's distribution is rarely discussed, as it is believed to only occupy the western part of Java, while its conservation status is Near Threatened (NT) as of 15 October 2018 on the IUCN red list. Specimens of O. hubbsi were recorded from Bandung and Jakarta in the description, other reports for O. hubbsi are commonly from Southern Sumatra-Western Java (SWJ) ecoregion in Java. However, an unexpected discovery of the genus Oryzias in Semarang freshwater opens up opportunities for the hidden spread of O. hubbsi to Central Java, and especially to other ecoregions which is Central and East Java (CEJ). Morphological approaches with distinctive O. hubbsi characters, complemented by molecular approaches with O. hubbsi samples from the Bogor population, confirmed similarities of species between both populations. Genetic distance using p-distances between both populations was below 2%, both populations made obvious clusters with a gap of 0.5%, and differed by five nucleotide base sites only. This new distribution record of O. hubbsi can be a foundation for conservation and domestication planning efforts.
Strengthening Pro-Environmental Behavior through Methane Gas Utilization and Environmental Education at Talangagung Landfill Muhammad Zainush Sholihin; Hamamah Hamamah; Agung Pramana Warih Marhendra
AL-ISHLAH: Jurnal Pendidikan Vol 18, No 3 (2026): In Progress September 2026
Publisher : STAI Hubbulwathan Duri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35445/alishlah.v18i3.10093

Abstract

Despite growing attention to pro-environmental behavior, existing studies have largely examined environmental education and methane gas utilization separately, providing limited understanding of how their integration promotes sustainable behavioral change in landfill communities. This study investigates how environmental education integrated with methane gas utilization strengthens pro-environmental behavior among communities surrounding Talangagung Landfill, Indonesia. A qualitative case study was conducted at Talangagung Landfill involving 30 purposively selected participants, including local government officials, landfill managers, community members, and waste workers. Data were collected through semi-structured interviews, participant observation, and document analysis and analyzed using thematic analysis supported by data triangulation and member checking. Four interrelated dimensions emerged. First, environmental education enhanced environmental literacy, ecological responsibility, and long-term behavioral commitment. Second, methane gas utilization reduced household cooking fuel expenditure by IDR 50,000–100,000 per month while increasing participation in waste management activities. Third, community participation reinforced waste sorting, composting, waste bank engagement, and collective environmental maintenance through social learning. Fourth, institutional support strengthened program sustainability through infrastructure development, environmental regulations, and multi-stakeholder collaboration. However, unequal environmental literacy, infrastructure limitations, and inconsistent policy implementation remained significant challenges. The findings demonstrate that integrating environmental education with methane gas utilization creates complementary educational, economic, social, and institutional mechanisms that foster sustained pro-environmental behavior. The proposed Four-Pillar Model extends pro-environmental behavior theory by illustrating how economic incentives reinforce environmental learning and collective action, offering a transferable framework for community-based environmental education and sustainable landfill management.
Co-Authors Abdul Rahem Faqih Akhsan Fikrillah Paricahya Dewa Gede Raka Wiadnya HAMAMAH, M.Pd. Ifa Sufaichusan Kiki Nur Azam Kholil Kiki Nur Azam Kholil Muh. Herjayanto Muhammad Zainush Sholihin Nia Kurniawan Rengga Retno Laila Saputri Shima Ramadani