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Cloning and Clone Analysis of GRA1 Gene from Local Isolate Toxoplasma gondii Tachyzoite Subekti, Didik T; Artama, W.T; Sulistyaningsih, E; Poerwanto, S.H; Sari, Y; Bagaskoro, F
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 1 (2008)
Publisher : Indonesian Animal Sciences Society

The GRA1 gene of Toxoplasma gondii encoding protein called GRA1 protein. GRA1 protein known to be immunogenic and essentialy involved in modification of parasitophorus vacoule which has role in immune evasion and virulency of organism. The local isolate of T. gondii is successfuly isolated and known as highly pathogenic isolate similarly as its RH strain. Unfortunately, the homology sequence of GRA1 gene between those isolate still unknown. The purpose of the research are to clone the GRA1 gene and to analyze the homology from pathogenic T. gondii isolate and RH strain. Tachyzoite of T. gondii was grown in mice peritoneum by intraperitoneal injection. Then, total mRNA was isolated and purified. cDNA was synthesized from mRNA and then amplified using F1 dan R1 primers to get clone of GRA1 from local isolate. Homology analysis was perform using several bioinformatic softwares. The result showed that cDNA of GRA1 from local isolate has 84% homologs with RH strain of T.gondii. However, when subsequently editing performed to parts of suspected non coding sequence of cDNA GRA1 to get CDS of GRA1, the homology was increase to 100% compare to CDS of GRA1 of RH strain. Key words: GRA1, Toxoplasma gondii, Clonning, Expression

Cloning and Clone Analysis of GRA1 Gene from Local Isolate Toxoplasma gondii Tachyzoite Didik T Subekti; W.T Artama; E Sulistyaningsih; S.H Poerwanto; Y Sari; F Bagaskoro
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 1 (2008): MARCH 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The GRA1 gene of Toxoplasma gondii encoding protein called GRA1 protein. GRA1 protein known to be immunogenic and essentialy involved in modification of parasitophorus vacoule which has role in immune evasion and virulency of organism. The local isolate of T. gondii is successfuly isolated and known as highly pathogenic isolate similarly as its RH strain. Unfortunately, the homology sequence of GRA1 gene between those isolate still unknown. The purpose of the research are to clone the GRA1 gene and to analyze the homology from pathogenic T. gondii isolate and RH strain. Tachyzoite of T. gondii was grown in mice peritoneum by intraperitoneal injection. Then, total mRNA was isolated and purified. cDNA was synthesized from mRNA and then amplified using F1 dan R1 primers to get clone of GRA1 from local isolate. Homology analysis was perform using several bioinformatic softwares. The result showed that cDNA of GRA1 from local isolate has 84% homologs with RH strain of T.gondii. However, when subsequently editing performed to parts of suspected non coding sequence of cDNA GRA1 to get CDS of GRA1, the homology was increase to 100% compare to CDS of GRA1 of RH strain. Key words: GRA1, Toxoplasma gondii, Clonning, Expression

Comparative Analysis of Certification Organic and Non-Organic Rice Farming Income in East OKU District Masroka, B; Sari, Fifian Permata; Sari, Y
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 8 No. 2 (2024)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29165/ajarcde.v8i2.413

East OKU Regency is the second-largest rice-producing region in South Sumatra, following Banyuasin Regency, with a harvest area of 108,140.86 hectares in 2022. In recent years, the cultivation of certified organic rice has been developed in East OKU, particularly in East Buay Madang District and Belitang II District. These areas began developing organic rice in 2016 and received organic certification from INOFICE in 2020. This study examines the income differences between organic and non-organic rice farmers in East OKU Regency. The research was conducted in January 2024 in East OKU Regency using a survey method and disproportionate stratified random sampling. The sample consisted of 30 organic rice farmers out of 97 and 30 non-organic rice farmers out of 175 in Belitang Mulya District. Data analysis involved using an income equation to calculate farmers' income and the t-student test to determine the income differences between organic and non-organic rice farmers. The results show that the income of certified organic rice farmers in East OKU Regency is IDR 28,791,050 per hectare per year, or IDR 48,758,30 per hectare per year, which is significantly higher than the income of non-organic rice farmers, who earn IDR 11,334,887 per hectare per year, or IDR 24,574,281 per hectare per year. The difference in income between certified organic and non-organic rice farming in East OKU Regency is IDR 17,456,163 per hectare per year, or IDR 24,184,022 per hectare per year.

Analysis of Income and Level of Satisfaction of Farmer Groups with UPPB OKU Regency Septiana, A; Sari, Y; Sari, Fifian Permata; Julkhaidar, Julkhaidar
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 8 No. 2 (2024)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29165/ajarcde.v8i2.415

OKU Regency is a significant smallholder rubber-producing region, covering an area of 72,010 hectares as of 2023. The presence of UPPB (joint processing and marketing units) in OKU Regency has greatly facilitated local rubber farmers' marketing of Bokar. UPPB plays a crucial role in enhancing the income of rubber farmers in the region. This study investigates farmer groups' income levels and satisfaction associated with UPPB in OKU Regency. The research employs a survey method with a simple random sampling technique. It involves 77 rubber farmers from a total of 350 UPPB members in OKU. Conducted in November 2023, the study focuses on Peninjauan District, the central hub of UPPB activities and the rubber auction market in OKU. Data analysis is performed using a quantitative descriptive method, applying an income equation to evaluate the income of rubber farmers participating in the UPPB.

Determination of Household Food Consumption Patterns in East Baturaja District OKU Regency Veranita, L; Munajat, Munajat; Sari, Y; Sari, Fifian Permata
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 8 No. 2 (2024)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29165/ajarcde.v8i2.395

The income level will influence household spending patterns. In conditions of limited income, low-income or poor households will prioritise allocating their income to buying food. This income will be used to spend on food for survival so it will affect the quantity and quality of household food consumption. Good quality food consumption indicates that nutritional adequacy levels are met. This research analyses the factors influencing household food consumption patterns in East Baturaja District, OKU Regency. This research used a survey method and was carried out in three sub-districts in East Baturaja District, OKU Regency, namely Kemala Raja, Sekar Jaya and Tanjung Baru Villages. The method for determining respondents is random sampling using the Parell et all (1973) formula with the statement if the variance of the total population is unknown, so the best way to determine the sample is to use a percentage of the entire population, namely 2%, 5%, 10%, 20% or 50% of the population. The population is 2126 families, obtained by 2%, namely 42.52 and determined to be 43 families. This research used multiple linear regression models to determine the factors influencing food consumption patterns using multiple linear analyses. Based on the research results, it can be concluded that the factors that influence household food consumption patterns in East Baturaja District, OKU Regency, are income (X1), number of family members (X2), age (X3), and education level (X4). The variable that has the most influence on household food consumption patterns in East Baturaja District, OKU Regency, is the income level variable (X1). Simultaneously or together, the income variable (X1), number of family members (X2), age (X3), and education level (X4) has a significant influence. R2 showed this with a value of 97.9%.

Strategy for Developing Aquaculture Villages in East OKU Regency Irawan, D; Sari, Y; fifi, Fifian Permata Sari; Novayanti, Novayanti
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 8 No. 2 (2024)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29165/ajarcde.v8i2.397

East OKU Regency, located in South Sumatra, is one of the largest catfish producers through pond cultivation. Recently, the Ministry of Marine Affairs and Fisheries designated East OKU Regency as a center for catfish farming, a status formally recognized by officials from South Sumatra and East OKU Regency. The catfish cultivation village spans 1,200 hectares and produces 30 tons annually. To ensure sustainable and environmentally friendly aquaculture practices, and to enhance fish farmers' incomes, effective development strategies are necessary. This research explores strategies for developing aquaculture villages in East OKU Regency. The study employs a case study method, focusing on Belitang District as a representative example of an aquaculture village in South Sumatra Province. A simple random sampling method was used to select 90 farmer groups from a population of 900 fish farmers. The research data were processed and analyzed using SWOT analysis to identify strategies for advancing fish farming villages in East OKU Regency. The findings suggest that the optimal development strategy (SO Strategy) involves providing training and skills related to technology and production systems to boost catfish output. Additionally, strengthening cultivator group institutions is crucial for facilitating the adoption of product quality standards and improving production quality to meet market demands.

Comparative Analysis of Certification Organic and Non-Organic Rice Farming Income in East OKU District Masroka, B; Sari, Fifian Permata; Sari, Y
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 8 No. 2 (2024)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29165/ajarcde.v8i2.413

East OKU Regency is the second-largest rice-producing region in South Sumatra, following Banyuasin Regency, with a harvest area of 108,140.86 hectares in 2022. In recent years, the cultivation of certified organic rice has been developed in East OKU, particularly in East Buay Madang District and Belitang II District. These areas began developing organic rice in 2016 and received organic certification from INOFICE in 2020. This study examines the income differences between organic and non-organic rice farmers in East OKU Regency. The research was conducted in January 2024 in East OKU Regency using a survey method and disproportionate stratified random sampling. The sample consisted of 30 organic rice farmers out of 97 and 30 non-organic rice farmers out of 175 in Belitang Mulya District. Data analysis involved using an income equation to calculate farmers' income and the t-student test to determine the income differences between organic and non-organic rice farmers. The results show that the income of certified organic rice farmers in East OKU Regency is IDR 28,791,050 per hectare per year, or IDR 48,758,30 per hectare per year, which is significantly higher than the income of non-organic rice farmers, who earn IDR 11,334,887 per hectare per year, or IDR 24,574,281 per hectare per year. The difference in income between certified organic and non-organic rice farming in East OKU Regency is IDR 17,456,163 per hectare per year, or IDR 24,184,022 per hectare per year.

Analysis of Income and Level of Satisfaction of Farmer Groups with UPPB OKU Regency Septiana, A; Sari, Y; Sari, Fifian Permata; Julkhaidar, Julkhaidar
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 8 No. 2 (2024)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29165/ajarcde.v8i2.415

OKU Regency is a significant smallholder rubber-producing region, covering an area of 72,010 hectares as of 2023. The presence of UPPB (joint processing and marketing units) in OKU Regency has greatly facilitated local rubber farmers' marketing of Bokar. UPPB plays a crucial role in enhancing the income of rubber farmers in the region. This study investigates farmer groups' income levels and satisfaction associated with UPPB in OKU Regency. The research employs a survey method with a simple random sampling technique. It involves 77 rubber farmers from a total of 350 UPPB members in OKU. Conducted in November 2023, the study focuses on Peninjauan District, the central hub of UPPB activities and the rubber auction market in OKU. Data analysis is performed using a quantitative descriptive method, applying an income equation to evaluate the income of rubber farmers participating in the UPPB.

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