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Selection of Coconut Resistance to the Nutfall Disease (Phytophthorapalmivora Butler) The objective of this research was to select the coconut resistance to nutfall phytophthora disease. Using wounding inoculation method, detachedhits of 11 coconut populations at Pakuwon Coconut Cropping Pattern Research Station were inoculated with Phytophthora palmivora Co5 isolate. Based on the dhease lesion size at seven days after inoculation, Genjah Salak (GSK) coconutpopulation was more resistant than the Semuel D. Runtunuwu; Meity S. Sinaga; Alex Hartana
Buletin Hama dan Penyakit Tumbuhan Vol. 11 No. 1 (1999): Buletin Hama dan Penyakit Tanaman
Publisher : Buletin Hama dan Penyakit Tumbuhan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Selection of Coconut Resistance to the Nutfall Disease (Phytophthorapalmivora Butler) The objective of this research was to select the coconut resistance to nutfall phytophthora disease. Using wounding inoculation method, detachedhits of 11 coconut populations at Pakuwon Coconut Cropping Pattern Research Station were inoculated with Phytophthora palmivora Co5 isolate. Based on the dhease lesion size at seven days after inoculation, Genjah Salak (GSK) coconutpopulation was more resistant than the other coconutpopulations and showed individual resistance variation to the disease. From 238 GSK coconut trees selected, 226 (95%) trees were resistant and 12 (5%) trees were susceptible.
PENGARUH WAKTU PENYEMPROTAN DAN KONSENTRASI PACLOBUTRAZOL (PBZ) TERHADAP PERTUMBUHAN DAN PRODUKSI TANAMAN JAGUNG (Zea mays L.) VARIETAS MANADO KUNING Bonaventura R. Lienargo; Semuel D. Runtunuwu; Johannes E.x. Rogi; Pemmy Tumewu
COCOS Vol. 4 No. 1 (2014)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/cocos.v4i1.3485

Abstract

This research was conducted at the experimental farm of the Faculty of Agriculture UNSRAT in the Pandu District Mapanget for 4 months, starting from the month Novenber 2012 to February 2013, which aims to determine the effect of spraying application time and concentration of paclobutrazol (PBZ) on the growth and yield of maize varieties Yellow Manado. The parameters measured were plant height, cob length, cob diameter, cob weight, number of rows cob, grain weight per ear. The results showed that the effect of concentration and time of application of paclobutrazol interact affect Manado Yellow corn cob weight, whereas high concentrations singly affect plants and spraying time singly affect the diameter of cob and grain weight per ear. Ear length and number of lines is not affected either concentration or time of spraying paclobutrazolKeyword: Spraying Application Time, Concentration Of Paclobutrazol (PBZ), Maize Varieties Yellow Manado
PENGARUH INTERAKSI KONSENTRASI DAN WAKTU APLIKASI PACLOBUTRAZOL TERHADAP PERTUMBUHAN DAN HASIL TANAMAN PADI SAWAH (Oryza sativa L) VARIETAS SERAYU Dellyna Rombon; Jemmy Najoan; Semuel D. Runtunuwu
COCOS Vol. 10 No. 5 (2018)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/cocos.v1i3.24364

Abstract

ABSTRACT This study aims to determine the effect of the interaction between the concentration and time of application of paclobutrazol to the growth and yield of wetland rice, The experiment was carried out for 4 months in Sulu Village , Tatapaan Indah District, South Minahasa Regency, starting from May to August 2014. The experiment was carried out using the Randomized Block Design (RBD) method, with two treatment factors namely paclobutrazol concentration, consisting of three concentrations namely: 1). P1 (0 ppm), 2). P2 (400 ppm), 3). P3 (600 ppm), and Time of paclobutrazol application (W), namely: 1). W1 is sprayed at plant age 30 HST, 2). W2 is sprayed at plant age 37 HST, 3). W3 is sprayed at plant age 44 HST. Observations were made on: Plant height, number of productive tillers, harvested dry grain, paddy filled with paddy, weight of paddy filled with clumps. Data were analyzed by variance and the smallest real difference test was BNT 0.5%. The time and concentration of administration of paclobutrazol only interact significantly in plant height. The concentration of paclobutrazol (singly) has a significant effect on: 1). Dry paddy harvest, 2). Number of grains containing clumps, 3). grain weight containing clumps. Keywords: paddy, paclobutrazol, concentration, date of application, interaction.
KONSENTRASI PACLOBUTRAZOL DAN PERTUMBUHAN TINGGI BIBIT CENGKEH (Syzygium aromaticum (L.) Merryl & Perry) Semuel D. Runtunuwu; Rinny Mamarimbing; Pemmy Tumewu; Tommy Sondakh
EUGENIA Vol. 17 No. 2 (2011)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.17.2.2011.3535

Abstract

ABSTRACT The purpose of this research was to study the effect of the concentration of growth inhibitor Paclobutrazol (Pbz) against the high growth of clove seedlings (Syzygium aromaticum (L.) Merryl & Perry) and get Pbz concentrations that can produce seed cloves in a relatively short (dwarf). The results showed that spraying Paclobutrazol (Pbz) with 25-100 ppm affect height growth of clove seedlings. Pbz the higher concentration applications, the shorter the length of apical segments produced shoots. In other words, the higher the concentration Pbz, the higher the emphasis on high growth of clove seedlings. Pbz emphasis on high growth of clove seedlings varied from the lowest, ie. 3.79% (25 ppm) up to the highest, ie 69.85% (100 ppm). Based on the comparison of height increment of plants sprayed with Paclobutrazol, the dose applications can be used to get the seed cloves that dwarf is a concentration of 50 to 100 ppm. Key Words :  Paclobutrazol, Gibberellin, Clove, Dwarfism
RESPON PERTUMBUHAN TINGGI DAN PRODUKSI TANAMAN CENGKEH (Syzigium aromaticum L.) TERHADAP PEMBERIAN PACLOBUTRAZOL Frieda F. Moningka; Semuel D. Runtunuwu; Jeanne M. Paulus
EUGENIA Vol. 18 No. 2 (2012)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.18.2.2012.3565

Abstract

ABSTRACT   This research aims to produce a shorter cloves and generate more interest and qualified by the presence of paclobutrazol treatment. The results showed that the high accretion paclobutrazol can produce the content of chlorophyll a (0,032 mg g-1) and chlorophyll b (0,004 mg g-1), dry weight of 1000 grains (207 g) and dry weight / plant (6.038 kg) was 8 years old clove higher than the control. Recommended further research to determine the maximum effect of paclobutrazol application on plants. Paclobutrazol at a dose of 2.5 g / tree height increment can deliver maximum results of cloves. Keywords  : Cloves, gibberallin, paclobutrazol
EFEKTIFITAS WAKTU PEMBERIAN DAN KONSENTRASI PACLOBUTRAZOL TERHADAP PERTUMBUHAN DAN HASIL KENTANG (Solanum tuberosum L.) VARIETAS SUPEJOHN Frangki Sambeka; Semuel D. Runtunuwu; Johannes E.X. Rogi
EUGENIA Vol. 18 No. 2 (2012)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.18.2.2012.3566

Abstract

ABSTRACT   The study aimed to examine the influence of time of application and concentration of paclobutrazol on growth and yield of potato crop Supejohn held in District Modoinding South Minahasa regency, from March to June 2012, using a randomized block design with two factors. The first factor is the concentration of Paclobutrazol with four standards are: 0 ppm, 75 ppm, 100 ppm, 125 ppm and the second factor is the provision of a three-level applications MST 4, 5 and 6 MST MST (Week After Planting). Based on research, the results of analysis of variance showed an interaction between application timing and concentration of paclobutrazol. Combination treatment with 125 ppm paclobutrazol 6 WAP application time can give significant effect on plant height, number of chlorophyll content, percentage weight class tuber yield components in the form of increased crop production amounted to 45.04 kg / plot (24 plants) / range 52. ton / ha by pressing the vegetative growth of plants. Key words : Supejohn, time application, concentration, paclobutrazol
PERSENTASE BUAH JADI PADA PERSILANGAN KELAPA GENJAH SALAK DENGAN KELAPA DALAM MAPANGET (GSK x DMT) Semuel D. Runtunuwu; Jantje Pongoh; Heldering Tampake; Yefta Pamandungan
EUGENIA Vol. 13 No. 1 (2007)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.13.1.2007.7429

Abstract

ABSTRACT Runtunuwu, S.D., et.al. 2007. The Become Fruit Rate in Crossing Coconut Genjah Salak x Dalam Mapanget (GSK x DMT). Eugenia 13(1):97-108. The aim of this research was 1) individu tree crossing of coconut Genjah Salak x Dalam Mapanget (GSK x DMT), 2) to known the become fruit rate in crossing individu tree of coconut GSK x DMT. The method of crossing used crossing a pair between fourteen trees individu of coconut GSK. With three trees coconut DMT, so it become fourty two combination crossing. Coconut DMT 1188 crossing with stem number one of coconut GSK, DMT 1172 with stem number two coconut GSK and DMT 781 with stem number three. The become fruit rate of the crossing result between fourteen individu trees of coconut GSK and tree individu trees of DMT with the tree number 1188, 1172 and 781 in three mounth in sequence was have variation from 0,00 – 27,27 %, 3,76 – 32 % and 2,86 – 27,16 %. Therefore the lowest success rate of these crossing was in combination of GSK coconut tree number fourteen and DMT tree number 1188 was 0,00 %, while the highest success rate was in the combination crossing between coconut GSK tree number 12 and DMT tree number 1172 was 32 %. Keywords: coconut, fruit rate, crossing
STUDI KERAGAMAN POLA PITA DNA DAN KESERAGAMAN POPULASI KELAPA GENJAH SALAK (GSK) BERDASARKAN PENANDA RAPD Novarianto Hengky; Semuel D. Runtunuwu; Edy F. Lengkong; Merlyn Singkoh
EUGENIA Vol. 13 No. 1 (2007)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.13.1.2007.7430

Abstract

ABSTRACT Novarianto, H., et.al. 2007. The Study of the Variety of DNA Tape Form and the Coconut Genjah Salak Population Variety Based on RAPD Code. Eugenia 13 (1):109-118.   The goal of this research to study the variety of DNA tape form and the population variety of coconut Genjah Salak (GSK) based on RAPD code (Randomly Amplified Polymorphic DNA). 25 trees individu of coconut GSK was amplification the genomic of DNA using 35 primer Operon Kit A and Kit B. The coconut GSK individu tree that were analyse was selected based on the female flower production, with average above 40 female flower. The electrophoresis of DNA result of amplication PCR get tape form monomorfic and polimorfic between 25 individu coconut GSK trees that was analyzed. OPA 09 primer and OPB 06 get the result of monomorfic tape form, other wise the OPA 13 primer, OPB 05 and OPB 10 get polimorfic tape form. The genetic similarity in coconut Genjah Salak (GSK) population based on RAPD code rate was 88 % or have the variety genetic of 12 %. Therefore based of this similarity the coconut GSK population was clustering in five groups. The goal of this research to study the variety of DNA tape form and the population variety of coconut Genjah Salak (GSK) based on RAPD code (Randomly Amplified Polymorphic DNA). 25 trees individu of coconut GSK was amplification the genomic of DNA using 35 primer Operon Kit A and Kit B. The coconut GSK individu tree that were analyse was selected based on the female flower production, with average above 40 female flower. The electrophoresis of DNA result of amplification PCR get tape form monomorfic and polimorfic between 25 individu coconut GSK trees that was analyzed. OPA 09 primer and OPB 06 get the result of monomorfic tape form, other the OPA 13 primer, OPB 05 and OPB 10 get polimorfic tape form. The genetic similarity in coconut Genjah Salak (GSK) population based on RAPD code rate was 88 % or have the variety genetic of 12%. Therefore based of this similarity the coconut GSK population was clustering in five groups. Keywords: Keragaman genetic; kelapa Genjah Salak (GSK), Randomly Amplified Polymorphic DNA (RAPD)
STUDI PERAKITAN KELAPA HIBRIDA GSK x DMT BERDASARKAN PENANDA RAPD (RANDOMLY AMPLIFIED POLYMORPHIC DNA) Semuel D. Runtunuwu; Hengky Novarianto; Heldering Tampake; Edy F. Lengkong
EUGENIA Vol. 14 No. 1 (2008)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.14.1.2008.7431

Abstract

ABSTRACT   Runtunuwu, S.D. et al. 2008. Assembling Hybrid Coconut of GSK x DMT Based on RAPD (RANDOMLY AMPLIFIED POLYMORPHIC DNA) Marker. Eugenia 14 (1) : 134-152.   The aimed of this research was : 1. assembling hybrid coconut GSK x DMT (Genjah Salak x Dalam Mapanget) that seeds growth was relatifly homogeneous based on RAPD (Randomly Amplified Polymorphic DNA) marker and 2. to found the assembling method of hybrid coconut that will produce massive seeds relatifely short time will homogeneous plant. It was 65 individu trees observe for the average of famale flower per bunch. The result was 25 individu of coconut GSK has the average flower production > 40 per bunch was analyze the homogeneous genetic with the RAPD marker. Based on the analyze RAPD that were 25 individu of GSK coconut trees have the same genetic average 88 % and 14 individu among that was 100 % have same genetic. Further more that 14 individu of GSK was crossing with the 3 individu of DMT that have high yield per year its was DMT 1188, 1172 and 781. Based on the evaluation for the color of buds, high of buds, the steam circle, the petiole color and the germination time of hybrid coconut seeds from the crossing of GSK x DMT 1188 produce more than    70 % seeds that have same genetic, also for crossing of GSK x DMT 1172 have 9 combination and have more than 70 % that same genetic, 10 combination from crossing GSK x DMT 781 have more than 80 % same seeds growth. Therefore, using the RAPD marker were successfully produced 28 crossing of the hybrid coconut GSK x DMT that have relatifly homogeneous seeds growth.   Keywords : assembling, hybrid coconut GSK x DMT, RAPD.
IDENTIFIKASI PENANDA ISOZIM PER-7, PER-8, DAN RAPD OB17375 PADA KELAPA GENJAH SALAK (GSK) DAN BEBERAPA HASIL SILANGANNYA DENGAN KELAPA DALAM Semuel D. Runtunuwu; Eddy F. Lengkong
EUGENIA Vol. 11 No. 1 (2005)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.11.1.2005.11882

Abstract

ABSTRACT Runtunuwu, S.D. and E.F. Lengkong. 2005. Identification of Isozyme Markers (PER-7, PER-8, and RAPD OBI7375) of Dwarf Salak Coconut (GSK) and its Tall Hibrids. Eugenia 11 (1): 8-17. The objective of this research was to identify PER-7 and PER-8 isozymes, and OB17 375 RAPD as molecular markers for resistance to NF Phytophthora disease in Genjah Salak (GSK) dwarf coconut and its several hybrids with Tall coconut at The Research Institute for Coconut and Palmae (RICP) Manado. Coconut resistance to NF Phytophthora disease was determined based on disease lesion size of inoculated coconut fruits at 7 days after inoculation. PER-7 and PER-8 isozymes marker were identified using horizontal starch gel electrophoresis, and OB17375 RAPD marker was identified based on polymerase chain reaction (PCR) using kit B number 17 primer (Operon Technologies, California). PER-7 isozyme marker could be used to select GSK x DTA (Dalam Tenga) and GSK x RLT (Rennell Tall) hybrid coconuts that resistant to NF Phytophthora disease. PER-7 isozyme without OB17375 markers (PER-7/-) could be used to select resistant GSK x WAT (West African Tall) hybrid coconut to the disease. Keywords: Isozymes, RAPD, Molecular marker, Phytophthora disease, coconut