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All Journal Agrosains: Jurnal Penelitian Agronomi Biosaintifika: Journal of Biology & Biology Education Agrotechnology Research Journal Journal of Biodiversity and Biotechnology
Ahmad Yunus
Department of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret
Agriculture, Biological Sciences & Forestry

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Size and Density of Artemisia annua Stomata Soaked in Water Extract of Gloriosa superba Seeds Sri Indah Rahmawati; Ahmad Yunus; Ari Susilowati
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11234

Abstract

Artemisia annua is a herbaceous plant that produces artemisinin as a malaria drug, haemorrhoids therapy, aromatherapy, antiviral, anticancer and antibacterial. Gloriosa superba is a plant that contains high colchicine compounds, especially on the seeds. Gloriosa superba extracts of tubers, stems, seeds, and leaves were used as biomutagen for many plants. Colchicine contains of these plants as antimitotic have been studied and proven by the mitotic index plants. Water extracts of Gloriosa superba seeds was used as a mutagen for Artemisia annua. The aim of this study was to determine the size and density of Artemisia annua stomata soaked in water extract of Gloriosa superba seeds as a mutagen. Extraction of Gloriosa superba seeds obtained naturally on Krakal Beach, Gunung Kidul by using a maceration method with water solvent (1:1). Artemisia annua sprouts were obtained from B2P2TOOT Tawangmangu. Variables treatment on sprouts using water extract concentration of Gloriosa superba seeds and soaking time of Artemisia annua sprouts. Measurements of stomatal length, width and density were conducted in epidermis of Artemisia annua leaf. Observation and measurements of the stomata were conducted by using a light microscope. The results showed that the length and width of stomata were 0.025 mm and 0.017 mm respectively. The stomatal density of the control leaf (174.69 amount/mm2) was lower than the other treated plants. Stomatal size and density has increased with the increasing concentration extracts on treated plants. Water extracts of Gloriosa superba seeds proved the effects on stomatal size and density of treated plants.  
In Vitro Propagation of Tribulus terrestris with IAA and BAP Concentrations Samanhudi Samanhudi; Ahmad Yunus; Rahmanto Rahmanto
Journal of Biodiversity and Biotechnology Vol 1, No 1 (2021)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v1i1.50245

Abstract

Tribulus terrestris is conventionally propagated using seeds but has limited germination capacity. One approach that can be done for this plant multiplication is in vitro tissue culture. This study was conducted to determine the growth potential of T. terrestris in vitro by the addition of auxin (IAA) and cytokinin (BAP) as growth regulators at several concentrations. The study was conducted at the Laboratory of Plant Physiology and Biotechnology, Faculty of Agriculture, Universitas Sebelas Maret Surakarta from February to December 2017. The experiment employed factorial Completely Randomized Design (CRD) method with 2 factors, namely IAA concentration (0 ppm, 0.1 ppm, 0.2 ppm, 0.3 ppm), and BAP concentration (0 ppm, 0.3 ppm, 0.5 ppm, 0.7 ppm). The observed growth parameters were shoot emergence time, leaf emergence time, root emergence time, number of leaves, and number of roots. Data were analyzed using analysis of variance followed by DMRT with a 95% confidence level. The results showed that the addition of IAA only affects leaf emergence time, while the addition of BAP had a significant effect on the leaf emergence time and number of leaves. The interactions between IAA and BAP had a significant effect on leaf emergence time. Keywords: explant; plant growth; tissue culture.
The Effect of Concentration and Time Interval of Kinetin Application on The Growth of Daun Duduk (Desmodium triquetrum L.) Seeds Asty Pratista Magdhalena; Bambang Puji Asmanto; Sulandjari Sulandjari; Ahmad Yunus
Journal of Biodiversity and Biotechnology Vol 1, No 1 (2021)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v1i1.50418

Abstract

Daun duduk (Desmodium triquetrum L.) is a medicinal plant that has the power to treat hemorrhoids, but has not been widely cultivated. This research was conducted to obtain the right concentration and time interval of kinetin application to increase the growth of Daun duduk seedlings. This research was conducted from November 2019 until March 2020 at the Screen House, Faculty of Agriculture, UNS. The factors of the experiment were concentration of kinetin (0 ppm, 10 ppm, 20 ppm and 30 ppm) and the time interval of application (once every three months, once every 1.5 months and once a month). The parameters observed in this study were plant height, stem diameter, number of leaves, number of branches, number of roots, root length, fresh stover and dry stover. The results showed that the single factor of kinetin concentration could increase plant height, stem diameter, number of leaves, number of branches and dry stover. Meanwhile, the time interval for application and the interaction between the two factors did not have any significant effect. The most effective kinetin concentration in increasing the growth of Daun duduk was 30 ppm. Keywords: growth regulators; herbal medicines; plant height.
Co-Authors Agastya Putra Pradipta Ari Susilowati Asty Pratista Magdhalena Bambang Puji Asmanto Bambang Pujiasmanto Dwi Harjoko Fajar Nugroho Glora Jayantie Hadiandra Harfiansyah Herjuna Praba Wisesa Herlina Mega Puspitasari Himawan Joko Riswanda Muji Rahayu Pricillia Galuh Pusparini Putri Dewangga Maharani Raden Dirgori Kuneng Brojokusumo Rahmanto Rahmanto Samanhudi Samanhudi Sri Hartati Sri Indah Rahmawati Sulandjari Sulandjari Yuli Widiyastuti