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All Journal HAYATI Journal of Biosciences Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Indonesian Journal of Biotechnology Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biodjati Biosaintifika: Journal of Biology & Biology Education JPSCR : Journal of Pharmaceutical Science and Clinical Research Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) Medica Hospitalia SEMAR

ARI SUSILOWATI

Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Sebelas Maret

Author-ID : 231756

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Articles

BIOCHEMICAL CHARACTERIZATION OF RECOMBINANT PHYTASE ENZYME (phyK) FROM Klebsiella sp. ASR1 ENCAPSULATED WITH ALGINATE Muhammad Eka Hidayatullah; . Sajidan; Ari Susilowati; Baraka Stewart Mkumbe; Ralf Greiner
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Karakterisasi Biokimia Enzim Fitase Rekombinan (phyK) dari Klebsiella sp. ASR1 Yang Dienkapsulasi Dengan AlginatEnzim fitase melepas molekul fosfor pada atom C dari benzena Inositol fitat. Tetapi fitase memiliki kelemahan tidak mampu bertahan terhadap kondisi ekstrim dalam lambung nonruminansia. Solusi dalam penelitian ini yaitu fitase dienkapsulasi menggunakan alginat. Penelitian ini bertujuan mengkarakterisasi fitase setelah dienkapsulasi menggunakan alginate. Hasil penelitian ini yaitu fitase yang dienkapsulasi memiliki aktivitas tertinggi pada pH 6,0, sedangkan fitase tanpa enkapsulasi pada pH 5,0. Suhu optimum untuk aktivitas tertinggi fitase yang dienkapsulasi yaitu 70ÂºC, sedangkan fitase tanpa enkapsulasi 37ÂºC. Untuk perlakuan penambahan ion logam, aktivitas tertinggi fitase yang dienkapsulasi terjadi dengan penambahan 0,1 mM Fe2+ dan 1,0 mM Ca2+, sedangkan fitase tanpa enkapsulasi dengan penambahan 0,1 mM Fe2+. Berdasarkan hasil penelitian ini, fitase yang dienkapsulasi memiliki keunggulan lebih banyak dibandingkan dengan fitase tanpa enkapsulasi, karena mampu bertahan pada pH dan suhu tinggi, dan beberapa efek ion logam.Kata Kunci: alginat, asam fitat, enkapsulasi, fitase, fitase rekombinanABSTRACTPhytase enzymes release phosphorus molecules on the C atom from benzene inositol phytate. But phytase has the disadvantage of being unable to withstand extreme conditions in the non-ruminant stomach. The solution in this research was phytase encapsulated using alginate. This study aims to characterize phytase after being encapsulated using alginate. The results of this study were the encapsulated phytase had the highest activity at pH 6.0, while the unencapsulated phytase at pH 5.0. The optimum temperature for the highest activity of the encapsulated phytase was 70ÂºC, while the unencapsulated phytase 37ÂºC. For treatment of metal ion addition, the highest activity of the encapsulated phytase occurred with the addition of 0.1 mM Fe2+ and 1.0 mM Ca2+, while the unencapsulated phytase with the addition of 0.1 mM Fe2+. Based on the results of this study, the encapsulated phytase had more advantages compared to the unencapsulated phytase, as the former withstand high pH and temperature, and some metal ion effects.

Polyurethane Degrading Bacteria Isolated from Decayed Teak Wood (Tectona grandis Linn. f.) Farah Aldila; Ari Susilowati; Ratna Setyaningsih
Jurnal Biodjati Vol 4, No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.4525

Polyurethane is a class of polymers characterized by their substantial tensile strength and high melting points which enables them to be extremely durable. Despite its xenobiotic origin, polyure-thane has been found susceptible to biodegradation by microorgan-isms. The main object of this research was to investigate polyurethane degradation by the bacterium isolated from decayed teak wood (Tec-tona grandis Linn. f.). Polyurethane degrading bacteria were isolated using selective medium in the form of LB (Luria Bertani) with the ad-dition of 0.3% Impranil. Bacterial biodegradation activities shown by the changes of polyurethane structure analyzed by FT-IR spectrosco-py. The bacterial identification was carried out based on observations on the morphological characteristics of the colonies, cell morphology and sequences of 16S rRNA encoding genes. One of the potential iso-late that successfully isolated was K9, which demonstrates the disap-pearance of the 1735/cm peak of the characteristic function urethane in the FT-IR analysis. Analysis of the 16S rRNA encoding gene showed that the potential isolate having 98% similarity index to Bacillus safen-sis strain FO-36b. The activity shown by the isolate suggests that the bacteria could be a promising agent for polyurethane degradation.

Sistem Pendukung Konsep Diri Dan Kualitas Hidup Pasien Kanker Payudara Di RSUP Dr Kariadi Semarang Nurul Azizatunnisa; Ari Susilowati
Medica Hospitalia : Journal of Clinical Medicine Vol. 5 No. 1 (2018): Med Hosp
Publisher : RSUP Dr. Kariadi

Latarbelakang :Kanker payudara dapat mempengaruhi konsep diri dan kualitas hidup pasien. Diperlukan sistem pendukung yang berasal dari sumber eksternal (dukungan keluarga) dan internal pasien. Dukungan keluarga akan membantu meningkatkan konsep diri pasien kanker payudara.Sumber internal pasien dapat berasal dari kelompok swabantu atau SelfHelp Group(SHG) yang beranggotakan pasien dengan jenis penyakit sama sehingga dapatsaling memotivasi untuk meningkatkan kualitas hidup.Tujuan :Artikel penelitian ini bertujuan untuk mengetahui sistem pendukung konsep diri dan kualitas hidup pasien kanker payudara di RSUP Dr. Kariadi Semarang.Metode :Terdapat dua jenis penelitian kuantitatif dalam artikel ini,yaituÂ desain penelitian kuantitatif non eksperimental metode crosssectional daneksperimental one group pre-test post-test design. Populasi dan sampel penelitian adalah pasien kanker payudara yang dirawat di RSUP Dr. Kariadi pada bulan Oktober - Desember 2014. Metode samplingyang digunakan adalah total sampling dan purpossivesampling. Instrumen ini terdiri dari empat kuesioner yang berisi data demografi, kuesioner konsep diri, kuesioner dukungan keluarga dan WHO-QoL BREFuntuk mengukurkualitas hidup pasien. Analisa data meliputi analisa univariat yang menggambarkan karakteristik responden dengan distribusi frekuensi serta analisa bivariat menggunakan uji RankSpearmandan Mann Whitney U.Hasil :Hasil analisa data menggunakan RankSpearmen diperoleh nilai p = 0,000, p value< 0,05, maka ada hubungan antara dukungan keluarga dengan konsep diri pasien kanker payudara di RSUP dr Kariadi Semarang. Hasil penelitiandalam artikel ini juga menunjukkan bahwa 93,3% responden mengalami peningkatan pada domain fisik dan 100% mengalami peningkatan pada domain psikologis, hubungan sosial dan lingkungan.Kesimpulan :Dengan demikian SHGberpengaruh terhadap kualitas hidup pasien kanker payudara di RSUP Dr. Kariadi Semarang.Â Kata Kunci : kanker payudara, sistem pendukung,konsep diri, kualitas hidup

Production, Characterization, and Molecular Phylogenetic Analysis of Phytase from Aspergillus niger Isolates of an Indonesia Origin Baraka Stewart Mkumbe; Sajidan Sajidan; Artini Pangastuti; Ari Susilowati; Ralf Greiner
HAYATI Journal of Biosciences Vol. 29 No. 4 (2022): July 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.4.468-478

This research aimed at analyzing the phytase from fungal isolates SB1, SB2, BS, and WF produced in cornstarch with glucose medium (CS+Glu) as carbon sources and Potatoes dextrose broth (PDB). The activity of phytase from isolates SB1, SB2, BS, and WF produced in CS+Glu medium was 2.97 UmL-1, 2.87 UmL-1, 3.18 UmL-1, and 4.37 UmL-1, respectively, while the activity of phytases was 2.07 UmL-1, 2.17 UmL-1, 2.22 UmL-1, and 2.78 UmL-1 respectively in PDB medium. The optimal temperature of SB1 and WF phytase was 40°C, while SB2 and BS were 50°C and 60°C, respectively. The optimal pH of SB1 and WF phytase was 5.0, while SB2 and BS phytase were 6.0, and 4.0 respectively. 18S rRNA gene analysis revealed that SB1 was 99% identical to Aspergillus niger ANTS (KY825168.1), SB2, BS, and WF were 99% identical to A. niger Moriga leaf (MG889596.1). Multiple sequences and phylogenetic analysis of phytase gene showed that phyA_SB1 and phyA_SB2 were 98% homology with A. ficuum (AAB26466), 97% with A. niger (ADP05107) while phyA_WF was 99% with A. ochraceoroseus (PLB29348), 98% with A. niger (ADP05105). The deduced proteins contain conserved motifs RHGARYPTD at N-terminal while lacking HD motif at C-terminal. These phytases were in the same cluster with Aspergillus sp. phytase A indicating that they belong to Histidine Acid Phosphatases (HAP) family.

Efektivitas enzim kasar kitinase dari jamur Trichoderma viride yang diiradiasi oleh sinar gamma terhadap degradasi cangkang telur nematoda Haemonchus contortus pada ternak domba Devi Indrawati; Ari Susilowati; Dadang Priyo Atmojo; Nana Mulyana
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 29, No 1 (2019): April
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2019.029.01.04

The aim of this study was to determine increasing of chitinase crude enzyme activity produced by Trichoderma viride at different gamma irradiation doses and to know the degradation on the eggshell of Haemonchus contortus. Eggs of Haemonchus contortus were isolated from sheep feces using buoyant centrifugation method. The qualitative test of chitinase was conducted by inoculating Trichoderma viride irridiated by variation gamma doses control, 250 Gray, 500 Gray, 750 Gray and 1000 Gray on the media of colloidal chitin agar. The quantitative test was conducted by measuring chitinase activity using colorimetric method based on specific enzyme activity and determination of dissolved protein levels. Shell changes were observed microscopically while N-acetyl glucosamine levels and dissolved protein levels were measured using spectrophotometer. Data were analyzed using One Way Analysis of Variance (ANOVA) with significance of the 95% and continued with Duncan’s Multiple Range Test (DMRT test). The results showed that gamma irradiation doses treatment increase of chitinase crude enzyme activity produced by Trichoderma viride. At a dose of 500 Gray, Trichoderma viride had the optimum chitinase specific activity i.e. 0.30 U/mg. Furthermore, chitinase crude enzyme effective to degrade all layers of the eggshell of Haemonchus contortusand the higher the concentration of crude enzyme given, the greater the level of egg damaged.

Variasi Genotipe dan Alel Gen PIT1|HinfI pada Sapi Perah Friesian Holstein Lokal di Boyolali Jawa Tengah: Genotype and Allelic Variations of the PIT1|HinfI Gene in Local Friesian Holstein Dairy Cows in Boyolali, Central Java Sigit Prastowo; Shavya Sarah Saviera; Galih Pambuko; Rebecca Vanessa; Purwadi Purwadi; Ari Susilowati; Sutarno Sutarno
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol. 12 No. 1 (2022): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Ve
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v12i1.201

Abstract The pituitary-specific transcription factor 1 (PIT1) gene, known also as pituitary-specific positive transcription factor 1 (POU1F), is one of the genes which has the responsibility to control milk quality and milk production. Using this gene information as selection criteria were expected to be able to improve milk production in an efficient and accurate way. This study was aimed to determine the genotype variation of the PIT1 gene in Friesian Holstein (FH) dairy cattle in Boyolali District Central Java. In total 20 blood samples as DNA source were collected from local FH cattle. To determine the PIT1 genotype and allele variation, Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method was employed which started by DNA extraction, PCR, then DNA digestion using HinfI restriction enzyme. Following the genotyping process, genotype and allele frequencies were calculated. As the result, it was found 3 types of PIT1 genotype namely AA, AB, and BB; its frequencies were 0.1, 0.3, and 0.6, respectively. The allele type was found A and B, and the frequencies were 0.25 and 0.75 respectively. According to the study, it is concluded that the highest genotype of PIT1 in local FH dairy cattle was BB type, and the allele was B type. Keywords: Genotype and allele frequency; Local FH dairy cattle; PIT1 gene Abstrak Gen Pituitary-Specific Transcription Factor 1 (PIT1) atau dikenal juga dengan nama pituitary-specific positive transcription factor 1 (POU1F1), merupakan salah satu gen yang bertanggung jawab pada kualitas dan kemampuan produksi susu sapi. Oleh karena itu, seleksi menggunakan gen ini diharapkan dapat meningkatkan produksi susu secara akurat dan efisien. Tujuan dari penelitian ini adalah untuk menentukan variasi genotip gen PIT1 pada sapi perah Friesian Holstein di Kabupaten Boyolali, Jawa Tengah. Penelitian ini menggunakan metode Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) untuk menentukan variasi genotipe dan alel gen PIT1. Sampel yang digunakan berasal dari darah 20 ekor sapi Friesian Holstein (FH) lokal di wilayah Kabupaten Boyolali, Jawa Tengah. Penelitian diawali dengan ekstraksi DNA yang dilanjutkan dengan amplifikasi fragmen DNA gen PIT1 pada reaksi PCR. Genotyping dilakukan dengan mendigesti produk PCR menggunakan enzim restriksi HinfI. Analisis variasi genotipe kemudian digunakan untuk menentukan frekuensi genotipe dan frekuensi alel gen PIT1. Hasil amplifikasi fragmen gen menghasilkan produk PCR dengan ukuran 451 bp dan berdasarkan hasil sequencing merupakan fragmen gen PIT1 ekson 6. Sebanyak 3 genotipe gen PIT1 yang terdeteksi pada populasi sampel sapi FH lokal yaitu AA, AB, dan BB dengan frekuensi genotipe masing-masing 0,1; 0,3; dan 0,6. Hasil frekuensi alel yang diperoleh untuk alel A dan B masing-masing sebesar 0,25 dan 0,75. Berdasarkan penelitian ini maka dapat disimpulkan bahwa gen PIT1 pada populasi sapi perah FH lokal di Boyolali memiliki frekuensi genotipe terbesar yaitu BB, dengan frekuensi alel terbesar adalah B. Kata kunci: Frekuensi genotipe dan alel; Gen PIT1; Sapi friesian holstein lokal

Diversifikasi Tanaman Pekarangan Dengan Tanaman Alpukat Untuk Meningkatkan Gizi Keluarga Sri Hartati; Ahmad Yunus; Nandariyah Nandariyah; Endang Yuniastuti; Bambang Pujiasmanto; Edi Purwanto; Samanhudi Samanhudi; Sulandjari Sulandjari; Adi Ratriyanto; Sigit Prastowo; Ida Rumia Manurung; Venty Suryanti; Ari Susilowati; Anif Nur Artanti; Sri Mulyani; Paramasari Dirgahayu
SEMAR (Jurnal Ilmu Pengetahuan, Teknologi, dan Seni bagi Masyarakat) Vol 11, No 2 (2022): November
Publisher : LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/semar.v11i2.61199

Alpukat (Persea americana) merupakan buah yang sudah sangat dikenal dan digemari oleh masyarakat. Alpukat diketahui memiliki khasiat sebagai antioksidan, antidiabetik dan efek hipolipidemik. Nutrisi yang terkandung dalam buah alpukat mampu membantu memenuhi kebutuhan nutrisi harian tubuh. Namun, cara budidaya tanaman Alpukat sangat berpengaruh pada khasiat atau kualitas dan kuantitas dari produk yang dihasilkan. Sehingga diperlukan pemahaman tentang cara budidaya tanaman Alpukat kepada masyarakat. Tujuan yang ingin dicapai dalam kegiatan ini adalah agar masyarakat lebih memahami cara budidaya tanaman alpukat sebagai tanaman pekarangan dan mengetahui manfaat buah Alpukat dalam meningkatkan gizi keluarga. Kegiatan pengabdian sudah dilaksanakan dengan metode penyuluhan dengan penyampaian materi tentang budidaya tanaman Alpukat, manfaat Alpukat, serta berbagai olahan Alpukat sedangkan pada akhir sesi dilakukan pemberian bibit Alpukat serta pupuk pada masing-masing peserta. Kegiatan yang sudah terlaksana ini bermanfaat untuk menambah pengetahuan dan keterampilan masyarakat khususnya masyarakat Kelurahan Wonokarto tentang budidaya tanaman Alpukat dan keanekaragaman manfaat Alpukat untuk kesehatan.Kata Kunci: alpukat; budidaya; penyuluhan; pupuk

Formulasi dan Pengujian Aktivitas Antibakteri Sediaan Gel Hand Sanitizer Berbahan Aktif Kitooligosakarida Diah Ayu Wardhani; Ari Susilowati; Artini Pangastuti
JPSCR: Journal of Pharmaceutical Science and Clinical Research Vol 8, No 1 (2023)
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jpscr.v8i1.64231

Kitooligosakarida merupakan derivat dari kitosan yang memiliki sifat antimikroba dan dijadikan bahan aktif dalam cairan hand sanitizer. Hand sanitizer bebas alkohol dapat menghindari terjadinya toleransi bakteri terhadap bahan kimia seperti alkohol dan mengurangi efek buruk alkohol bagi kulit. Penelitian bertujuan untuk mengetahui karakteristik sediaan hand sanitizer kitooligosakarida dan efektivitasnya dalam membunuh mikroba. Serbuk kitooligosakarida ditambahkan dalam formulasi hand sanitizer dengan variasi berat 1 g (formula 1), 1,5 g (formula 2), dan 2 g (formula 3) menggunakan basis NaCMC, propilenglikol, essens, dan metil paraben. Evaluasi stabilitas fisik hand sanitizer berdasarkan warna, tekstur, aroma, pH, homogenitas, dan daya sebar. Uji daya hambat hand sanitizer diuji melalui metode disc diffusion dilanjutkan dengan perbandingan daya hambat melalui pengukuran diameter zona penghambatan dan uji one-way ANOVA. Uji penurunan angka koloni mikroba dengan metode swabbing dan dilanjutkan perbandingan rerata penurunan koloni mikroba melalui uji T berpasangan. Hasil evaluasi stabilitas fisik hand sanitizer memenuhi kriteria gel yang baik. Hasil uji formula 1 memiliki kriteria angka diameter zona hambat yang lemah pada S. aureus (14,33 cm) dan daya hambat sedang pada E. coli (17,33 cm), Formula 2 memiliki angka diameter zona hambat kuat sebesar 21,33 cm pada S. aureus dan 20,33 cm pada E. coli. Formula 3 menghasilkan zona hambat kuat, yaitu 20,67 cm pada S. aureus dan 22,33 cm pada E. coli. Hasil penurunan angka koloni mikroba sediaan hand sanitizer paling besar yaitu formula 3 sebesar 80,22%. Hand sanitizer kitooligosakarida menunjukkan kemampuan penurunan angka koloni mikroba dan dapat digunakan sebagai formulasi alternatif hand sanitizer.

Antibacterial activity of bioactive compound produced by endophytic fungi isolated from Mangifera casturi Kosterm endemic plant from South Kalimantan, Indonesia Fatia Rizki Nuraini; Ratna Setyaningsih; Ari Susilowati
Indonesian Journal of Biotechnology Vol 28, No 2 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.71150

The endophytic fungi that live in endemic plants are a promising bio‐prospect as the producers of antibacterial compounds. This research is aimed to evaluate the endophytic fungi antibacterial compound from Mangifera casturi. The bioactive compounds of 13 endophytic fungi were extracted using ethyl acetate and evaluated for antibacterial activity using disk diffusion assay. The minimum inhibitory concentration (MIC) was measured using the serial broth dilution method. Scanning Electron Microscopy (SEM) was used to examine cell damage because of the extract. The antibacterial compounds were then detected using GC‐MS analysis. The endophytic fungi were identified morphologically and molecularly based on ITS rDNA sequencing Among the 13 isolates, the endophytic fungi identified as Botryosphaeria rhodina AK32 produced the antibacterial compounds that exhibited the highest activity and a broad spectrum. Moreover, they were capable against resistant bacteria, Methicillin Resistant Staphylococcus aureus (MRSA) with an MIC value of 1.56% for all the test bacteria. The mechanism of action of AK32 ethyl acetate extract seemed to affect the condition of bacterial cell walls, causing morphological alteration such as shrinkage of the cell, warted cells, and hollow cells. Based on GC‐MS, the antibacterial compounds of AK32 ethyl acetate extract were di‐n‐octyl phthalate, benzyl alcohol, high‐oleic (CAS) safflower oil, benzene acetonitrile, and benzotriazole.

Size and Density of Artemisia annua Stomata Soaked in Water Extract of Gloriosa superba Seeds Sri Indah Rahmawati; Ahmad Yunus; Ari Susilowati
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11234

Artemisia annua is a herbaceous plant that produces artemisinin as a malaria drug, haemorrhoids therapy, aromatherapy, antiviral, anticancer and antibacterial. Gloriosa superba is a plant that contains high colchicine compounds, especially on the seeds. Gloriosa superba extracts of tubers, stems, seeds, and leaves were used as biomutagen for many plants. Colchicine contains of these plants as antimitotic have been studied and proven by the mitotic index plants. Water extracts of Gloriosa superba seeds was used as a mutagen for Artemisia annua. The aim of this study was to determine the size and density of Artemisia annua stomata soaked in water extract of Gloriosa superba seeds as a mutagen. Extraction of Gloriosa superba seeds obtained naturally on Krakal Beach, Gunung Kidul by using a maceration method with water solvent (1:1). Artemisia annua sprouts were obtained from B2P2TOOT Tawangmangu. Variables treatment on sprouts using water extract concentration of Gloriosa superba seeds and soaking time of Artemisia annua sprouts. Measurements of stomatal length, width and density were conducted in epidermis of Artemisia annua leaf. Observation and measurements of the stomata were conducted by using a light microscope. The results showed that the length and width of stomata were 0.025 mm and 0.017 mm respectively. The stomatal density of the control leaf (174.69 amount/mm2) was lower than the other treated plants. Stomatal size and density has increased with the increasing concentration extracts on treated plants. Water extracts of Gloriosa superba seeds proved the effects on stomatal size and density of treated plants.

Co-Authors . Sajidan Adi Ratriyanto Ahmad Yunus Ahmad Yunus Amikom Rinayanti Anif Nur Artanti Artini Pangastuti Artini Pangastuti Bambang Pujiasmanto Baraka Stewart Mkumbe Baraka Stewart Mkumbe Dadang Priyo Atmojo Devi Indrawati Diah Ayu Wardhani Edi Purwanto Endang Yuniastuti Farah Aldila Fatia Rizki Nuraini Galih Pambuko Ida Rumia Manurung Muhammad Eka Hidayatullah Mulyani, Sri Nana Mulyana Nandariyah Nandariyah Nurul Azizatunnisa Paramasari Dirgahayu Purwadi Purwadi Ralf Greiner Ralf Greiner Ratna Setyaningsih Ratna Setyaningsih Rebecca Vanessa Sajidan Sajidan Samanhudi Samanhudi Shavya Sarah Saviera Sigit Prastowo Sigit Prastowo Sri Hartati Sri Indah Rahmawati Sulandjari Sulandjari Sutarno Sutarno Tri Ariyati Venty Suryanti

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