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Januarsih Januarsih
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Dentistry Environmental Science Health Professions Medicine & Pharmacology Nursing Public Health

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EFEKTIFITAS EKSTRAK PUNICA GRANATUM TERHADAP KADAR NITRICT OXYDE PADA KULTUR HUVECS YANG DIPAPAR PLASMA PREEKLAMPSI Megawati Megawati; Januarsih Januarsih; Fitria Jannatul Laili
Jurnal_Kebidanan Vol. 15 No. 2 (2025): Jurnal Kebidanan Volume 15 Nomor 2 Tahun 2025
Publisher : STIKES Panca Bhakti Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33486/jurnalkebidanan.v15i2.384

Abstract

Preeclampsia is one of the leading causes of maternal morbidity and mortality, with pathogenesis involving oxidative stress due to increased free radicals and decreased antioxidant activity. Red pomegranate (Punica granatum) contains polyphenols, anthocyanins, and tannins, which act as natural antioxidants. This study aimed to investigate the effect of Punica granatum extract on Nitric Oxide (NO) levels in Human Umbilical Vein Endothelial Cells (HUVECs) exposed to preeclampsia plasma. An experimental study with a post-test only control group design was conducted with five groups: negative control (HUVECs + normal plasma), positive control (HUVECs + preeclampsia plasma), and three treatment groups with Punica granatum extract at 14 ppm, 28 ppm, and 56 ppm. NO levels were measured using the ELISA method after 24 hours of incubation. The results showed significant differences among groups (p < 0.05). The 56 ppm extract dose resulted in the most significant decrease in NO levels (20.3 µM), comparable to the normal control group (18.4 µM). This study is at TRL 3–4, representing a proof-of-concept based on in vitro testing. In conclusion, Punica granatum extract has potential as a natural antioxidant and a complementary therapy candidate for preeclampsia, although further studies in animal models and clinical trials are required.
Perbedaan Kadar Hemoglobin Ibu Hamil antara Wilayah Endemis dan Non-Endemis Cacing Buski di Kabupaten Hulu Sungai Utara Januarsih Januarsih; Rita Kirana; Agus Rachmadi
Jurnal Skala Kesehatan Vol. 17 No. 1 (2026): Jurnal Skala Kesehatan Edisi Januari 2026
Publisher : Politeknik Kementerian Kesehatan Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jsk.v17i1.518

Abstract

Anemia during pregnancy remains a major public health issue in Indonesia. One of the contributing factors is intestinal parasitic infection, such as (buski fluke), particularly in areas with poor sanitation and raw aquatic plant consumption. To compare hemoglobin levels among pregnant women in endemic and non-endemic areas of Fasciolopsis buski. This was a comparative cross-sectional study involving 124 pregnant women, consisting of 62 from endemic and 62 from non-endemic regions. Data were obtained through interviews, observations, and hemoglobin measurements using Hemocue®. Data were analyzed using an independent t-test at a 0.05 significance level. The mean hemoglobin level among pregnant women in endemic areas was 10.7 ± 1.1 g/dL, while in non-endemic areas it was 11.6 ± 1.0 g/dL. The t-test revealed a significant difference between the two groups (p = 0.001). The prevalence of anemia was 61.3% in endemic and 29.0% in non-endemic areas. There is a significant difference in hemoglobin levels between pregnant women living in endemic and non-endemic areas of Fasciolopsis buski. Endemicity of parasitic infection may contribute to higher anemia risk among pregnant women in endemic regions.
Faktor Faktor Risiko Stress Meningkatkan Kejadian Premenstrual Syndrome (PMS) pada Mahasiswa Kebidanan Poltekkes Kemenkes Banjarmasin Nur Rohmah Prihatanti; Januarsih Januarsih; Megawati Megawati; Norlaila Sofia
JURNAL KEBIDANAN BESTARI Vol 6 No 1 (2022): Jurnal Kebidanan Bestari
Publisher : Midwifery Departement Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jkb.v6i1.89

Abstract

Premenstrual Syndrome (PMS) is a collection of moderate to severe affective and physical symptoms that develop during the luteal phase of the menstrual cycle and disappear within a few days of menstruation. Complaints of Premenstrual Syndrome (PMS) that often occur are anxiety, fatigue, difficulty concentrating, difficulty sleeping, loss of energy, headache, abdominal pain, and breast pain. The prevalence of Premenstrual Syndrome (PMS) reaches 90% of the entire population of women of childbearing age in the world. In Indonesia, it reaches 85% experienced by women of childbearing age. As many as 20-50% of them experience severe Premenstrual Syndrome (PMS) symptoms that interfere with daily activities. The incidence of Premenstrual Syndrome (PMS) can increase because it is influenced by several risk factors, one of which is stress. The study used an analytical observational research design with a cross sectional approach. The research was conducted at the Midwifery Department of the Health Poltekkes of the Ministry of Health Banjarmasin and carried out in August-December 2021 with a population of all third semester students of the three diploma study program majoring in midwifery Poltekkes Ministry of Health Banjarmasin for the 2021/2022 academic year, which is as many as 40 people. Sample selection using simple random sampling method. Samples were taken randomly from the third semester student population of the three diploma study program majoring in midwifery for the 2021/2022 academic year with the requirement that they have experienced menstruation. The sample was calculated using the Slovin formula and the number of samples was 35 people. The independent variable is stress with the research instrument in the form of a questionnaire and the dependent variable is Premenstrual Syndrome (PMS) with the research instrument in the form of a questionnaire The Shortened Premenstrual Assessment Form (SPAF). From the results of a simple linear regression test, the results of the calculation of p-value = 0.000 or the value of Sig. 0.000 < 0.05, which means that stress increases the incidence of Premenstrual Syndrome (PMS) in students of the Department of Midwifery, Poltekkes Kemenkes Banjarmasin. Keyword: Students, Premenstrual Syndrome (PMS), Stress
Co-Authors Agus Rachmadi Fitria Jannatul Laili Megawati Megawati Megawati Megawati Norlaila Sofia Nur Rohmah Prihatanti Rita Kirana