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All Journal HAYATI Journal of Biosciences Aquacultura Indonesiana
Ketut Maha Setiawati
Balai Besar Riset Perikanan Budidaya Laut, Gondol
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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The Use of Carotene Materials as the Source of Red Color Pigmentation on Leopard Grouper Larvae (Plectropomus leopardus) Kusumawati, Daniar; Setiawati, Ketut Maha
Aquacultura Indonesiana Vol 17, No 2 (2016)
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (722.686 KB) | DOI: 10.21534/ai.v17i2.50

Abstract

Carotenoid is one of nutrition factors which can improve red color pigmentation. The purpose of this study was to investigate the best type of carotene which can increase red pigment performance and to evaluate how long the effect should be retaining in order to maintain red color performance on leopard grouper seeds. This study consisted of 2 experiments; those were the effect of various kinds of carotene materials in feed addition and the effect of the termination of the addition of carotene materials in the feed toward red color performance on leopard grouper  seed. The treatments given to the first experiment were the provision of various kinds of carotene materials which were type of A. Haematococcus pluvialis, B. Phaffiarhodozyma, C. Astax Oil, D. Control (without carotene materials) and the treatments to the second experiment are A. Leopard grouper  seed – phaffia was still given additional Phaffia rodhozyma, B. Leopard grouper  seed – haematococcus was still given additional Haematococcus pluvialis, C. leopard grouper seed (from larvae - phaffia / haematococcus) was terminated from carotene materials addition. The first experiment was applied from larva up to D60 seed and for the second experiment was the follow-up response from the first experiment conducted on D60 seed up to D150. Based on the study result, it indicates that carotene materials of Haematococcus pluvialis type gave the best color performance improvement (P value 4.71 x 10-7 < 0.05). The provision of carotene materials intake decreases color performance both visually and total contain of carotene (P value 5.97 x 10-5< 0.05) (Tabel 1). Carotenenoids should be continuously given as trigger to maintain red color performance on leopard grouper  seed. There was protein band in the range of 82.1 – 84.2 kDa which was assumed as protein expression of astaxanthin
The Survival, Growth, and Accelerating Morphological Development of Stichopus horrens are Affected by the Initial Larval Stocking Densities Sembiring, Sari Budi Moria; Setiawati, Ketut Maha; Widiastuti, Zeny; Hutapea, Jhon Harianto; Gunawan, Gunawan; Setiadi, Ananto; Haryanti; Giri, Nyoman Adi Asmara
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.233-240

Abstract

Stichopus horrens is highly exploited due to their use as a pharmaceutical ingredient. Since then, this species has become extinct; therefore, it is necessary to start aquaculture. Gonad maturation and broodstock spawning succeeded, but the optimum larval-rearing stocking density has not yet been determined. Therefore, the present study aimed to determine the optimal stocking density to improve survival and accelerate the development of larval morphology. Three treatments were used: 100, 150, and 200 larvae L-1, each with four replicates. Twelve 100-litre plastic containers filled with 80 litres of seawater as larval rearing media were placed in a concrete tank with a water-bath system using a heater and a thermostat (29.0°C±1.0°C). The larvae were fed a mixture of microalgae (Chaetoceros muelleri, Isochrysis galbana, and Nitzchia sp.) twice a day-1. The data collected included survival and growth rates, larval morphological development, and water quality. The fastest metamorphose from auricularia to the doliolaria stage occurred in 100 larvae L-1, 15 days after hatching. The highest survival rate, growth rate, and percentage of larvae metamorphose to the doliolaria stage were obtained in the 100 larvae L-1 as 26.38%, 26.4 µm day-1, and 65.27%, respectively, and were significantly different (P<0.05). A stocking density of 100 larvae L-1 was optimal for promoting survival and growth and accelerating the morphological development of auricularia to the doliolaria stage.
Co-Authors Daniar Kusumawati Giri, Nyoman Adi Asmara Gunawan Gunawan Haryanti Jhon Harianto Hutapea Sari Budi Moria Sembiring Setiadi, Ananto Widiastuti, Zeny