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All Journal HAYATI Journal of Biosciences BERITA BIOLOGI
Widiastuti, Zeny
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences

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IDENTIFICATION AND PATHOGENICITY TEST OF SOME BACTERIA ISOLATED FROM WILD AND FARMED SPINY LOBSTER PANULIRUS HOMARUS Sudewi, Sudewi; Widiastuti, Zeny; Mastuti, Indah; Mahardika, Ketut
BERITA BIOLOGI Vol 18, No 3 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i3.3578

Abstract

The bacterial populations in the farming of spiny lobster could have either beneficial or harmful effects depending on the prevailing conditions. We designed this study to identify and to perform a pathogenicity test of some bacteria isolated from wild and farmed spiny lobsters Panulirus homarus. The adult farmed lobsters were obtained from Pangandaran and Lombok coastal areas, while the wild lobsters were collected from Lombok, with five lobsters for each location. The bacteria were isolated from the midgut, gill, hepatopancreas, and muscle tissues of the lobsters. The identification of the bacteria was carried out by molecular methods. Pathogenicity test was performed by intramuscular injection of 0.1 ml bacterial suspensions at the density of 7×106cfu/ml into each three adult apparently healthy lobsters for every eight bacterial isolates. Our study identified six bacterial isolates that exhibited high homology of a nucleotide sequence with Shewanella algae, Bacillus firmus, Vibrio alginolyticus, Tenacibaculum lutimaris, Pseudomonas sp. and Vibrio sp., while two isolates were reminded unidentified due to low nucleotide similarities (< 97%). The pathogenicity test showed that there was no mortality of lobsters injected with those bacterial isolates. This may because the dose of injection was too low to induce bacterial infection particularly for Vibrio, or the bacteria were not pathogenic for lobster or even have the potency as probiotic bacteria.   
The Survival, Growth, and Accelerating Morphological Development of Stichopus horrens are Affected by the Initial Larval Stocking Densities Sembiring, Sari Budi Moria; Setiawati, Ketut Maha; Widiastuti, Zeny; Hutapea, Jhon Harianto; Gunawan, Gunawan; Setiadi, Ananto; Haryanti; Giri, Nyoman Adi Asmara
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.233-240

Abstract

Stichopus horrens is highly exploited due to their use as a pharmaceutical ingredient. Since then, this species has become extinct; therefore, it is necessary to start aquaculture. Gonad maturation and broodstock spawning succeeded, but the optimum larval-rearing stocking density has not yet been determined. Therefore, the present study aimed to determine the optimal stocking density to improve survival and accelerate the development of larval morphology. Three treatments were used: 100, 150, and 200 larvae L-1, each with four replicates. Twelve 100-litre plastic containers filled with 80 litres of seawater as larval rearing media were placed in a concrete tank with a water-bath system using a heater and a thermostat (29.0°C±1.0°C). The larvae were fed a mixture of microalgae (Chaetoceros muelleri, Isochrysis galbana, and Nitzchia sp.) twice a day-1. The data collected included survival and growth rates, larval morphological development, and water quality. The fastest metamorphose from auricularia to the doliolaria stage occurred in 100 larvae L-1, 15 days after hatching. The highest survival rate, growth rate, and percentage of larvae metamorphose to the doliolaria stage were obtained in the 100 larvae L-1 as 26.38%, 26.4 µm day-1, and 65.27%, respectively, and were significantly different (P<0.05). A stocking density of 100 larvae L-1 was optimal for promoting survival and growth and accelerating the morphological development of auricularia to the doliolaria stage.
Co-Authors Giri, Nyoman Adi Asmara Gunawan Gunawan Haryanti Jhon Harianto Hutapea Ketut Maha Setiawati Ketut Mahardika Mastuti, Indah Sari Budi Moria Sembiring Setiadi, Ananto Sudewi Sudewi