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All Journal Journal of Aquaculture and Fish Health
Farid Mukhtar Riyadi
Brawijaya University
Agriculture, Biological Sciences & Forestry

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In Vitro Analysis of Antibacterial Activities of Curry Leaf (Murraya koenigii) Extract Towards Bacteria Edwardsiella tarda Arif Syaifurrisal; Arief Prajitno; Mohamad Fadjar; Farid Mukhtar Riyadi; Annisa Isti Fauziyyah
Journal of Aquaculture and Fish Health Vol. 10 No. 2 (2021): JAFH Vol. 10 No. 2 June 2021
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v10i2.25042

Abstract

This study analyzed the antibacterial activity of curry leaf extract (Murraya koenigii) on the growth of Edwardsiella tarda bacteria. This study aims to determine the bioactivity and antibacterial effectiveness of M. koenigii leaf extract against the growth of E.tarda bacteria. Inhibition test was carried out by delusion (MIC test) and diffusion (disc test) methods. MIC test used 5 variations of concentration: 1 mg/L, 10 mg/L, 100 mg/L, 500 mg/L and 1,000 mg/L on TSB (Tryptone Soya Broth) media; it was incubated for 24 hours. While the disc test used 5 variations of concentration: 100 mg/L, 200 mg/L, 300 mg/L, 400 mg/L and 500 mg/L on TSA (Tryptone Soya Agar) media and incubated for 2x24 hours. Chloramphenicol (5 mg/L)  was used as a positive control, and distilled water was used as a negative control.                    M. koenigii leaf extract contains natural bioactive; it was bacteriostatic antibacterial due to bacteria's growth after 48 hours incubation. The highest inhibition diameter of E.tarda was 7,20 mm at a concentration of 500 mg/L after 24 hours incubation. The highest inhibitory effectiveness was at a concentration of 500 mg/L with effectivity 56.3%, while it declined to 46,44% after 48 hours incubation.
Potential of Moringa (Moringa oleifera) leaf extract to inhibit the growth of pathogenic bacteria Edwardsiella tarda Farid Mukhtar Riyadi; Arief Prajitno; Mohamad Fadjar; Arif Syaifurrisal; Annisa Isti Fauziyyah
Journal of Aquaculture and Fish Health Vol. 10 No. 3 (2021): JAFH Vol. 10 No. 3 September 2021
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v10i3.25057

Abstract

This study analyzed the antibacterial activity of Moringa oliefera leaf extract against the growth of Edwardsiella tarda bacteria. This study aims to determine the antibacterial activity of Moringa leaf extract (M. oliefera) against the growth of E.tarda bacteria. Inhibition testing is done by diffusion method (disc test). The disc test used five variations of concentration of 75 mg/L, 150 mg/L, 225 mg/L, 300 mg/L, and 375 mg/L on TSA (Tryptone Soya Agar) media and incubated for 2x24 hours. As a positive control, an antibiotic in the form of chloramphenicol was used. (5 mg/L) Moreover, distilled water was used as a negative control.Moringa leaf extract (M. oliefera) contains natural active compounds, bacteriostatic antibacterials, due to decreased bacterial growth after 48 hours of incubation. The highest inhibition diameter of E. tarda was 12.95 mm at a concentration of 375 mg/L after 24 hours incubation and decreased by 11.02 mm after 48 hours incubation. The highest inhibitory effectiveness was at a concentration of 375 mg/L with an effectiveness of 58.8%, while the effectiveness of the decrease was 48.1% after 48 hours of incubation.
The Effect of Giving Croton Leaf Crude Extract (Codiaeum variegatum) as an Alternative to The Antibacterial of Edwardsiella tarda in Vitro Annisa Isti Fauziyyah; Arief Prajitno; Mohammad Fadjar; Arif Syaifurrisal; Farid Muchtar Riyadi
Journal of Aquaculture and Fish Health Vol. 10 No. 3 (2021): JAFH Vol. 10 No. 3 September 2021
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v10i3.25067

Abstract

Production problems related to aquaculture cannot be separated from diseases that can cause death in fish. Efforts to overcome the bacterial disease Edwardsiella tarda can be done by using natural materials such as the leaves of croton (Codiaeum variegatum). This study aimed to analyze the content of active compounds contained in croton leaf extract which is antibacterial against E. tarda bacteria. Inhibition test was performed by dilution method Minimum Inhibition Concentration (MIC test) and agar diffusion (disc test). The MIC test used five concentrations: 1 mg/L, 10 mg/L, 100 mg/L, 500 mg/L, and 1000 mg/L on Tryptone Soy Broth (TSB) media, while the disc test used five doses of croton leaf extract, namely: A (100 mg/L), B ( 200 mg/L), C (300 mg/L), D (400 mg/L) and E (500 mg/L), 2 controls (positive and negative), and three replications on Tryptone Soy Agar (TSA) media and incubated for 2x24 h. The positive control used chloramphenicol (5 mg/L) antibiotic. Croton leaf extract contains several compounds, namely saponins, flavonoids, tannins, steroids, and polyphenols, which are antibacterial and could kill the test bacteria with the highest average inhibition zone results obtained at a dose of E (500 mg/L) of 6.34 mm, while the lowest results were obtained at dose A (100 mg/L) of 3.12 mm.
Co-Authors Annisa Isti Fauziyyah Annisa Isti Fauziyyah Annisa Isti Fauziyyah Arief Prajitno Arief Prajitno Arief Prajitno Arif Syaifurrisal Arif Syaifurrisal Arif Syaifurrisal Mohamad Fadjar Mohamad Fadjar Mohammad Fadjar