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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Pharmaciana: Jurnal Kefarmasian Proceeding Biology Education Conference
Sismindari Sismindari
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health

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Cytotoxic effects of methanol extract isolated from Erythrina fusca Lour on cancer cell-lines Sismindari Sismindari
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 35, No 2 (2003)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Erythrina is a medicinal plant which is frequently used to treat cancer in Africa. People in Java, however, use Erythrina fusca (cangkring) to treat varicella and measles. Previous works demonstrated that the methanol extract of this plant's leaves induced DNA topoisomerase II mediated DNA cleavage. This activity has been used widely as a target to find anticancer medicine. In order to be scientifically proofed the activity, therefore, it is necessary to analyze directly on the cancer cell-lines. Objectives: To identify the cytotoxicity effect of methanol extract of E. fusca leaves against cancer cell-lines.Methods: Cytotoxicity analysis of methanol extract isolated from E. fusca leaves was carried out against myeloma and HeLa S-3 cancer cell-lines, and to normal mononuclear cell. The level of cytotoxicity was determined by calculating the level of IC50 which was based on the percentage of the cell death following the 24 hours incubation with the extract.Results: It was demonstrated that this methanol extract was cytotoxic to myeloma and HeLa S-3 cell-lines with the IC50 of 0.005 mg/ml and IC50 of 0.08 mg/ml respectively. The percentage of the cell death on treated normal mononuclear cell with the extract, however, was very much low 110%). This was similar to that on the DMSO treated cells.Conclusion: The methanol extract isolated from E. fusca leaves was demonstrated had a selective cytotoxicity effect, as indicated by the level of the IC50 which was higher to myeloma compared to HeLa S3 cell-line, and had much less cytotoxic on normal mononuclear cells.Key words: Erythrina fusca, cytotoxicity, cancer cell-lines, mononuclear cell
VALIDASI METODE ANALISA PENETAPAN KADAR EPIGALOKATEKIN GALAT DENGAN KROMATOGRAFI CAIR KINERJA TINGGI Nining Sugihartini; Achmad Fudholi; Suwidjiyo Pramono; Sismindari Sismindari
Pharmaciana Vol 4, No 2 (2014): Pharmaciana
Publisher : Universitas Ahmad Dahlan

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High Performance Liquid Chromatography (HPLC) was one of analytical methodscommonly used to determine the concentration of epigallocatechin gallate (EGCG) on green teaextract. The method must be validated in order to fit to its purpose. The aim of this research was toprove that the used method has selectifity, liniearity, precise, accurate and know limit of detection(LOD) and limit of quantification (LOQ) is acceptable. The selectivity of analytical method wasdetermined by calculating the resolution value between two peak. Data from 10 μg/mL and100 μg/mL with 5 replicates would give precition and accuration. Precition was known from CV value and accuration was known from recovery value in each concentration. Liniearity was knownfrom regression linear between concentration and wide area of peak. From regresion linear couldcalculate LOD and LOQ. Research show that method of analyse have selectificity withRs= 2.27>1.5; liniearity with r= 0.99; precision with CV 8.74% at concentration 200 µg/mL and3.74% at concentration 500 µg/mL; accuration with recovery 99.76% at concentration 200 µg/mLand 100.52% at concentration 500 µg/mL and the value of LOD is 33.28 μg/mL and LOQ is110.93 μg/mL.
AKTIVITAS KEMOPREVENSI EKSTRAK TEMU KUNCI (Boesenbergia pandurata) PADA KARSINOGENESIS KULIT MENCIT BALB/C TERINDUKSI RADIASI ULTRA VIOLET Shanti Listyawati; Sismindari Sismindari; Sofia Mubarika; Yosi B. Murti
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 9, No 1 (2012): Prosiding Seminar Nasional IX Biologi
Publisher : Universitas Sebelas Maret

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ABSTRAK   Temu kunci (B.pandurata) mengandung senyawa-senyawa yang berpotensi antikanker. Penelitian ini bertujuan untuk mengkaji efek kemoprevensi ekstrak etanolik rimpang temu kunci pada karsinogenesis kulit terinduksi ultra violet. Ekstraksi serbuk rimpang B. pandurata dilakukan dengan metode maserasi  menggunakan etanol. Hewan uji yang digunakan adalah mencit Balb/C betina umur 28 hari yang dicukur rambut punggungnya, dikelompokkan menjadi 5 kelompok perlakuan, yaitu kelompok kontrol dan empat  kelompok   perlakuan ekstrak etanolik temu kunci yang diberikan secara oral. Induksi karsinogenesis dengan paparan UV dosis 0,167 J/cm2/hari, sebanyak 60 paparan (5 kali paparan/minggu). Efek penghambatan karsinogenesis kulit  dipelajari pada tingkat insidensi dan multiplisitas kanker. Hasil uji menunjukkan bahwa ekstrak etanolik temu kunci mampu menurunkan angka insidensi dan multiplisitas kanker kulit pada mencit Balb/C terinduksi UV. Ekstrak tersebut berpotensi   dikembangkan sebagai agen kemoprevensi kanker.   Kata Kunci: Boesenbergia pandurata, kemoprevensi, karsinogenesis , ultra violet,
Morphological And Biochemical Responses Of Saccharum Spontaneum L. Accessions To Drought Stress Aminatun Munawarti; Taryono Taryono; Endang Semiarti; Sismindari Sismindari
Journal of Tropical Life Science Vol. 4 No. 1 (2014)
Publisher : Journal of Tropical Life Science

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Glagah (Saccharum spontaneum L.) has potential value as a crop species and may also be used in sugarcane breeding programs; however, this germplasm has not been extensively used in breeding programs, primarily in relation to improve drought tolerance. The objectives of this experiment were to evaluate the effect of drought stress initiated at vegetative growth stage on growth, leaf proline content and protein pattern of seven glagah accessions (BOT-53, BOT-54, BOT-60, BOT-77, BOT-78, BOT-84, and BOT-88). The plants were propagated from single node stalk segments in polybag in the field under non-stress condition for two months. The two month-old plants were then subjected to drought stress by withholding watering for eight weeks. Untreated control plants were watered every two days. Results indicated that drought stress reduced plant height, stalk diameter and green leaf number. On the other hand, there was a little difference between drought-stressed and control plants in terms of proline content. The protein pattern showed that drought stress caused a change in gene expression in the form of induction or repression of protein expression. A specific protein with a low range of molecular weight (Rf value about 0.647) showed constitutively expressed in accession BOT-53 but drought-inducible expressed in BOT-54. Keywords: Drought stress, glagah, proline, protein pattern, Saccharum spontaneum
Co-Authors Achmad Fudholi Aminatun Munawarti Endang Semiarti Nining Sugihartini SHANTI LISTYAWATI Sofia Mubarika Haryana Suwidjiyo Pramono Taryono, Taryono Yosi B. Murti