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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Sain Veteriner Bioma : Berkala Ilmiah Biologi JURNAL ANATOMI FISIOLOGI AL KAUNIYAH Jurnal Sains dan Teknologi Biosaintifika: Journal of Biology & Biology Education Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Jurnal Kedokteran Hewan

Agung Janika Sitasiwi

Fakultas Kedokteran Hewan, Universitas Udayana, Bali

Author-ID : 382478

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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Hubungan Kadar Hormon Estradiol 17-B dan Tebal Endometrium Uterus Mencit (Mus musculus l.) selama Satu Siklus Estrus Sitasiwi, Agung Janika
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 16, No 2 (2008): Vol. XVI, No. 2, Oktober 2008
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Research about animal reproduction can be observed from many aspects, i.e. oestrous cycle. The oestrouscycle is the manifestation of reproduction hormones changes, especially oestradiol 17- B. The oestradiol 17- B hormones causes changes of tissues structures of reproductive tracts. This research have been done toanalyze the correlation between changes of oestradiol 17- B hormones and uterine endometrial thicknessalong the oestrous cycles. The adult female Swiss Webster mice were used as laboratory animal. The phaseof oestrous cycle determined by vaginal smears. Blood and uterine samples collected in each oestrousphases. Hormones concentration counted with RIA, the thickness of endometrial determined on uterinehistological slides with paraffin methods and stained with HE. This search showed that a positivecorrelation between oestradiol 17- B hormones and uterine endometrial thickness along the oestrous cycle.It also showed that oestradiol 17- B hormones have a proliferative effects on reproductive tissues.

Korelasi Jumlah Folikel Ovarium dengan Konsentrasi Hormon Estrogen Mencit (Mus musculus) setelah Konsumsi Harian Tepung Kedelai selama 40 Hari Mardiati, Siti Muflichatun; Sitasiwi, Agung Janika
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 16, No 2 (2008): Vol. XVI, No. 2, Oktober 2008
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Soybeans contains phytoestrogen that influence reproductive process. Phytoestrogen administration withchronical dosage along growth periode cause the neuroendocrine system disturbance. Commonly in humanintake of phytoestrogen from soybeans or other soybeans product. So it is important to research beneficialand adverse effects of soybeans consumption with chronical dosage to reproductive process, particulary iscorelation between the sum of ovarium follicles and the estrogen level of mouse (Mus musculus) after dailyconsumption of soybeans along 40 days. The objective of this study was to know the corelation betweenthe sum of ovarium follicle and the estrogen level of mouse, by experimental design. Adult female micewere use as experimental animal, divided into two groups, there were control and treatment. Thegroup of treatment was administrated with 0,975 mg soybeans flour, it was contain Â± 0,00156 mgphytoestrogen. The control group was administreted only drinking water. The result of this study showsthat were not significantly difference between control and treatment group. It concluded the corelationbetween the sum of ovarium follicles and the estrogen level were not influenced with daily consumption offitoestrogen.

Efek Paparan Tepung Kedelai dan Tepung Tempe sebagai Sumber Fitoestrogen terhadap Jumlah Kelenjar Endometrium Uterus Mencit (Mus musculus L.) Sitasiwi, Agung Janika
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 17, No 1 (2009): Volume XVII, Nomor 1, Maret 2009
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Phytoestrogen has been known have many advantages in human health. It has estrogenic effect because it similarity in structure with natural estrogen. It action done by interact with estrogen receptor and inhibit the interaction of natural estrogen. The aim of this research was to evaluate the effect of soybean starch as phytoestrogen resources on reproduction of M. musculus, by the endometrial thickness and the sum ofendometrial glands aspects. The adult female Swiss Webster mice were used as animal treatment. The mice were treated with 0,975 mg/kg BW/day of soybean starch and 0,148g/kgBW/day. Uterine samples collected from every estrous phase made as histological slides with paraffin methods and stained with H.E.with 6 Î¼ in thickness. Anova with factorial design at 5% significances were used to analyzed the data. This search showed that there was no significances between control and treatment group so it can be concluded that the result were caused by the act of endogen estrogen in animal treatment.

Deteksi Ekspresi Protein Wnt4 pada Uterus Mencit (Mus musculus L.) dengan Metode Immunohystochemistry Sitasiwi, Agung Janika
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 24, No 1 (2016): Volume 24, nomor 1, tahun 2016
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Study to detect the expression of Wnt4 protein in the uterus of Swiss Webster mice with immunohistochemistry method has been done. Laboratory animals that were used are adult Swiss Webster mice weighing 25-30 grams. Pregnancy is determined by the presence of vaginal plug in female mice after breeding. Uterus was isolated on seventh days of gestation. The detection of protein expression was done by paraffin method using mouse anti-Wnt4 (Santa Cruz Biotechnology) as primary antibody with 1:250 in dilution. Visualization of Wnt4 protein was done by DAB as chromogen. The positive reaction to the primary antibody showed in perimetrium, myometrium, and endometrium in uterine tissue. The positive reaction to the primary antibody were expressed on blood vessels also. It could be concluded that the Wnt4 protein as signal molecule regulates the embryo development by circulation system. This study offer as source to study Wnt4 protein expression pattern as molecular marker in early development of organism. Â Keywords: Wnt4, immunohystochemistry, Mus musculus

Motilitas spermatozoa manusia setelah simpan beku dengan medium TES-Tris yolk citrat (TES-TYC) Djaelani, Muhammad Anwar; Sitasiwi, Agung Janika
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 17, No 1 (2009): Volume XVII, Nomor 1, Maret 2009
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

The aim of this research was to evaluate the effect of semencryopreservation using TES-Tris yolk citrat (TES-TYC) medium onmotility of the sperm. Semen fulfilling inclusion criteria with WHO 1999 criteria. The sperm motility was counted as initial data. The semen was then mixed with TES-TYC medium and cryopreserved in liquid nitrogen. After one mounth the semen was thawed and recount its sperm motility. Data obtained showed that the motility of post freezing sperm cryopreserved was lower compared to the motility of pre freezing sperm. It could be concluded that cryopreservation caused decrease integrity and death of sperm during cryopreservation, that showed the motility of post freezing sperm cryopreserved was lower compared to the motility of prefreezing sperm.

Co-Authors Agung Budiyanto Ahmar, Rasyidah Fauziah Ariyanto, Hana Fadhillah Baiq Miftahul Jannah Darsono, Aurel Elizabeth Edy Dharmana, Edy Fitriani, Ratih Nur Kasiyati Kasiati Khairunnisa, Najla Athallah Kusuma, Alfana Bagus Melati, Alfriyan Krisna Muhammad Anwar Djaelani Nagari, Ardelia Padma Ningtias, Putri Lestari Pambudi, Destiana Dwiridhaningsih Putra, Alfisah Gama Sijabat, Agave Chintya Gabriella Sitepu, Erlalana Hansel Christian Siti Muflichatun Mardiati Sri Isdadiyanto SUNARNO Suprihatih, Teguh Teguh Suprihatin Tyas Rini Saraswati Wayan Tunas Artama

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