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All Journal Jurnal Labora Medika Journal of Nursing and Health (JNH) Akper Yakpermas Banyumas
Bambang Supriyanta
Poltekkes Kemenkes Yogyakarta
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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PENGARUH HEMOLISIS DALAM SERUM TERHADAP AKTIVITAS ENZIM ALANIN AMINOTRANSFERASE (ALT) Iswari Nurmandari; Siti Nuryani; Bambang Supriyanta
Jurnal Labora Medika Vol 3, No 2 (2019): Jurnal Labora Medika
Publisher : Program Studi Teknologi Laboratorium Medik

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (265.814 KB) | DOI: 10.26714/jlabmed.3.2.2019.41-44

Abstract

Hemolisis yang dapat menggangu pemeriksaan laboratorium disebabkan karena adanya hemoglobin dalam serum sehingga dapat mempengaruhi hasil pemeriksaan. Tujuan dari penelitian ini untuk mengetahui pengaruh kadar hemoglobin dalam serum terhadap aktivitas enzim Alanin aminotransferase (ALT). Penelitian ini merupakan Eksperimen murni berupa sampel darah yang dibuat menjadi pool serum yang kemudian dibagi menjadi 6 kelompok. Masing-masing  kelompok ditambah hemolisat sebanyak 0 µl, 335 µl, 665 µl, 1000 µl, 1335 µl dan 1665 µl. Sehingga didapatkan kadar hemoglobin dalam 0 mg/dl, 85,2 mg/dl, 170,4 mg/dl, 231 mg/dl, 340,8 mg/dl dan 426,1 mg/dl. Kemudian diukur aktivitas enzim Alanin aminotransferase (ALT) dengan metode kinetik enzimatik. Hasil pemeriksaan akan diperoleh data sebanyak 42 data. Analisis deskriptif menujukan penurunan aktivitas enzim Alanin aminotransferase (ALT) pada serum yang mengadung hemoglobin. Uji ANOVA One-Way  menujukan adanya perbedaan aktivitas enzim Alanin aminotransferase (ALT) pada serum yang mengandung variasi hemoglobin. Uji korelasi menunjukan adanya hubungan yang sangat kuat antara kadar hemoglobin dalam serum terhadap pemeriksaan aktivtas enzim Alanin aminotransferase (ALT). Presentase penurunan aktivitas Alanin aminotransferase (ALT) karena kadar hemoglobin dalam serum sebesar 93,3 % dan 6,7% oleh faktor lain. Disimpulkan bahwa ada pengaruh hemolisis dalam serum terhadap aktivitas enzim Alanin aminotransferase (ALT).
PEMBUATAN ANTISERUM SALMONELLA O POLIVALEN MENGGUNAKAN ANTIGEN DAN KELINCI LOKAL Bambang Supriyanta; Ullya Rahmawati
Journal of Nursing and Health Vol. 9 No. 2 (2024): Journal of Nursing and Health
Publisher : Yakpermas Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52488/jnh.v9i2.365

Abstract

Introduction: Typhoid fever is a disease caused by infection with Salmonella enterica subspecies enterica serotype typhi (S. typhi). Laboratory diagnosis of typhoid fever by bacteriological examination in serological tests requires Salmonella O Polyvalent Antiserum reagent which has been imported, so it is attempted to make Salmonella O Polyvalent Antiserum independently using local antigens and rabbits. Objective: to create polyvalent Salmonella O antiserum using local antigens and rabbits. Methods: experimental research design posttest without control group. Cultures of S typhi, S paratyphi A, S paratyphi B, S paratyphi C, each processed, then mixed, so that it becomes Polyvalent Salmonella O Antigen. Polyvalent Salmonella O Antigen mixed with Freunds Complete Adjuvant, injected sub cutaneously (s.c) in rabbits, after one and two weeks the following week injected s.c Polyvalent Salmonella O Antigen plus Freunds Incomplete Adjuvant. After one week from the last injection, blood was taken through the ear artery (auricular artery) (collection I), one week after collection I, blood was taken (collection II), one week after collection II, the last blood was taken (Collection III), serum was made, the titer was measured using a suspension of Salmonella O Polyvalent Antigen with a turbidity of 0.5 Mc Farland standard. Result: Salmonella O Polyvalent was highest in rabbit blood collection in Collection II, indicating that the optimal production of antiserum at that time. Conclusion: The mean titer of Polyvalent Salmonella O Antiserum was highest in rabbit blood collection at Collection II.
Co-Authors Iswari Nurmandari Siti Nuryani Ullya Rahmawati