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All Journal Jurnal Natur Indonesia Jurnal Perikanan dan Kelautan Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Maspari Journal Forum Geografi BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Pendidikan Almuslim Makara Journal of Science
DEDI SOEDHARMA
Fakultas Ilmu Perikanan dan Kelautan IPB
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Social Sciences

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Biopotensi Tumbuhan Mangrove untuk Pencegahan Penyakit Vibrosis pada Udang Windu ., Melki; Soedharma, Dedi; Effendi, Hefni; Mustopa, A. Zaenal
Maspari Journal : Marine Science Research Vol 2, No 1 (2011): Edisi Januari
Publisher : UNIVERSITAS SRIWIJAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (307.912 KB) | DOI: 10.36706/maspari.v2i1.1146

Abstract

Ekstrak tiga jenis tumbuhan mangrove (daun, buah, batang dan akar) meliputi tumbuhan mangrove Ceriops tagal, Rhizophora apiculata, dan Sonneratia alba yang diambil dari Sadai, Bangka Selatan, Bangka Belitung dimana telah diekstraksi dengan pelarut methanol, ethyl acetate dan n-hexane serta telah diuji sebagai antibakteri pada bakteri Vibrio harveyi yaitu patogen udang windu dan uji toksisitas BSLT. Hasil uji antibakteri dari semua bagian tumbuhan mangrove didapatkan bahwa daun dan batang S. alba dengan pelarut metanol lebih potensi yaitu dengan zona hambat yang terbentuk 24±3,78 mm dan 23±3,78 mm. Hasil uji BSLT didapatkan untuk daun S. alba dengan pelarut metanol bersifat toksik sedangkan batang S. alba dengan pelarut metanol bersifat tidak toksik terhadap artemia. Selanjutnya bagian tumbuhan mangrove dianalisa komponen kimianya dengan menggunakan kromatografi kolom, kromatografi lapis tipis dan kromatografi cair kinerja tinggi dengan detektor PDA. Hasil identifikasi dengan menggunakan kromatografi kolom dengan eluen chloroform: methanol (9:1 sampai 1:9) memberikan hasil pemisahan terbaik, identifikasi dengan menggunakan kromatografi lapis tipis dengan eluen chloroform: methanol (9:1 sampai 1:9) memberikan hasil pemisahan terbaik dan identifikasi dengan kromatografi cair kinerja tinggi pada panjang gelombang 200-400 nm menghasilkan bahwa ekstrak tumbuhan mangrove yang berperan sebagai antibakteri dan untuk selanjutnya ekstrak tumbuhan mangrove ini dapat dikembangkan sebagai antibakteri dan biofarmatika.   Kata kunci: ekstrak mangrove, antibakteri, BSLT, kromatografi kolom , kromatografi lapis tipis, dan kromatografi cair kinerja tinggi ABSTRACT Crude extracts of three mangrove species (leaf, fruit, bark and root), i.e. Ceriops tagal, Rhizophora apiculata, and Sonneratia alba collected from Sadai, South Bangka, Bangka Belitung was extracted  in methanol, ethyl acetate, n-hexane and tested for different range of biological activities including antimicrobial activity of shrimp Vibrio harveyi pathogen and brine shrimp cytotoxic. The overall bioactivity profile showed that, leaf and bark of S. alba methanolic (24±3,78 mm and 23±3,78 mm) exhibited more biopotency. Result by using Brine Shrimp Lethality Test showed that leaf of S. alba methanolic was toxic and bark of S. alba methanolic was not toxic to Artemia. The highly active mangrove was evaluated further to analyze the chemical compounds using column chromatography, thin layer chromatography and high performance liquid chromatography with detector photodiode array (PDA). The identification with column chromatography using chloroform: methanol (9:1 to 1:9) gave the best separation, identification results using thin layer chromatography using chloroform: methanol (9:1 to 1:9) gave the best separation and chromatographic identification results high performance liquid at a wavelength of 200-400 nm showed mangrove extracts might have functional role in bioactivity and can be used for the development of antibacterial and biopharmaceuticals.   Keywords: Mangrove extract, antibacterial activity, brine shrimp cytotoxicity, column    chromatography, TLC, and HPLC
PRODUKTITVITAS DIATOM PERIFITIK YANG DITUMBUHKAN PADA TIPE SUBTRAT BERBEDA SEBAGAI ALTERNATIF PENYEDIAAN PAKAN ALAMI UDANG Pratiwi, Niken T.M.; Adiwilaga, Enan M.; Widigdo, Bambang; Soedharma, Dedi
JURNAL BIOLOGI INDONESIA Vol 4, No 3 (2007): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v4i3.3262

Abstract

ABSTRACTThe Productivity of Periphytic Diatom Grown on Different Types of Substrates asan Alternative on Providing Natural Feed for shrimp. Providing natural feed is anattempt to support fisheries culture in a polite way for the sustainability of environment.Periphytic community is living aquatic resources that have a potential value for thispurpose. The major component of saline water periphytic community is diatom.The study was emphasized on diatom presentation grown on two types of artificialsubstrates: biocrete (sand, palm fiber, cement) and zeocrete (zeolite, palm fiber, cement).The reseach was conducted in laboratory with an experimen design follows split splitplot in times. The aim of the research is to study the ecological factor and availablenutrients to support the growth of periphitic diatoms (Amphora, Cyclotella, Melosira,Navicula, Phaeodactylum, and Thallassiosira) on two different substrates. Theproductivity of diatom, and the effect of shrimp larvae on the diatom productivity werealso analyzed. Two fertilized materials (biocrete and zeocrete) with two main treatmentsand three levels of treatments each, and unfertilized materials were used as artificialsubstrates for periphitic diatoms. The results show that all treatment could give sufficientbiological available nutrient for the diatoms. The highest diatom productivity was achievedby the population on third level ratio of fertilized biocrete and zeocrete (added by fertilizedwith N:P ratio of 30:l). Diatom productivity follows the shrimp larvae grazing. Theshrimp larvae could grow well on the media with diatom that were grown on fertilizedbiocrete.Key words: periphytic diatoms, natural feed, biocrete, zeocrete, productivity
PENGARUH SUHU DAN SALINITAS TERHADAP RESPON FISIOLOGI LARVA TIRAM MUTIARA PINCTADA MAXIMA (JAMESON) Winanto, Tjahjo; Soedharma, Dedi; Affandi, Ridwan; Sanusi, Harpasis S.
JURNAL BIOLOGI INDONESIA Vol 6, No 1 (2009): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i1.3171

Abstract

ABSTRACTPENDAHULUANPinctada maxima adalah spesiesakuakultur yang mempunyai nilaiekonomi tinggi (Taylor et al. 1997). Dipasaran internasional, mutiara yangdiproduksi sering kali disebut dengannama ?South Sea Pearl?. Indonesiatermasuk salah satu negara penghasilmutiara (South Sea Pearl) yang cukupdiskenal di pasaran dunia, sebagian besarproduksi South Sea Pearl yang dipasarkanberasal dari hasil budidaya (Anna2006). Produksi mutiara berbasisbudidaya merupakan aktivitas usaha yangmenguntungkan. Perkembangan usahabudidaya mutiara saat ini sudah mengarahpada kegiatan industri yang terintegrasi(Fassler 1995).The Effect of Temperature and Salinity to The Physiological Respons on The Larvae ofPinctada maxima (Jameson). Energy budget is one of the most sensitive tools available forindividual assessing environmental changes like temperature and salinity, and also prerequisitefor individual growth and survival. The aim of this study is to obtained information on energybudget on routine metabolism, in different levels of temperature and salinity, and to know thelevels of optimum temperature and salinity. The research was used randomized block design,with three replications. The result showed that optimal temperature and salinity on P. maximalarvae was 28 oC and 32 ? 34 ? (BE and BF). Energy budget to routine metabolism increasedwas attributed to increased temperature and salinity due to the optimal, than would be decreasedwhen temperature and salinity increased. The highest of energy budged for routine metabolismat treatment BF. Stage I: energy budged between 6.73 ? 7.35 C g wet weight-1 hour-1 (28.18 ?30.74 J g wet weight-1 hour-1); Stages II: 5.85 ? 5.95 C g wet weight-1 hour-1 (24.48 ? 24.90 J g wetweight-1 hour-1); Stages III: 4.73 ? 4.80 C g wet weight-1 hour-1 (15.07? 19.58 J g wet weight-1hour-1). The highest survival rate of larvae was by treatment BF, but has not higher significant(P e? 0.05) with BE, stage I: survival rate between 87.75 ? 87.92 %; Stage II: 81.91 ? 82.39 % andstage III: 76.72 ? 77.26 %. The best of relative growth length of larvae by treatment BF and notsignificant (P e? 0.05) with BE, at stage I: 29.78 x 17.93 ?m ? 30.57 x 18.43 ?m (AP x DV); stageII: 57.62 x 46.73 ?m ? 58.13 x 47.33 ?m and stage III: 80.32 x 69.29 ?m ? 80.88 x 69.62 ?m. Thequickest time of plantigrade stages have found by treatment BF (day 19.50) and hasn?tsignificant (P > 0.05) with BE (day 20.85).Keywords: Pinctada maxima, larvae, response; physiology, metabolism.Kata kunci: Pinctada maxima, larvae, respon, fisiology, metabolisme.
KETERKAITAN STRUKTUR KOMUNITAS MEIOFAUNA DENGAN KERAPATAN LAMUN DAN PARAMETER LINGKUNGAN DI SELAT DOMPAK, KEPULAUAN RIAU Zulkifli, Zulkifli; Soedharma, Dedi; Wardiatno, Yusli; Sanusi, Harpasis S.
Jurnal Perikanan dan Kelautan Vol 12, No 01 (2007)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.12.01.15-22

Abstract

A study on the relationship between meiofauna community structure with seagrassdensity and environmental parameters has been carried out in the Dompak Straitwaters, Riau Archipelago. This study were investigated on four different seasonsthroughout a year, from May 2005 to April 2006. Three sampling site, i.e.., Al, A2and A3 were chosen to represent research sites. The sediment samples were tekenby PVC cores, in which six layers were separated (i.e. 0-5 cm, 5-10 cm, 10-15 cm,15-20 cm, 20-25 cm and 25-30 cm). The environmental parameters, such ascurrent velocity, the sediment composition, total organic carbon (TOO, totalorganic matter (TOM), pH and redox potential (Eh), were also observed. The dataon meiofauna, seagrass density and environmental parameters were analyzedstatistically by a regression analyisis. This analysis was run using a SPSS softwareversion 11.5 for Windows. The meiofauna was dominated by Nematodes,Copepods, Polychaetes, Foraniiniferans and Turbellarians. The results areshowing that the meiofaunal community structure would depend on severalconditions such as seagrass density, environmental factors and hydrologicalcharacteristics. The regression results are showing that the meiofaunal communitystructure patterns was significantly correlated with seagrass density and to theenvironmental parameters. Vertically, the meiofaunal community structurepatterns seems to be more affected by TOC and TOM. While the horizontally, itseems to he more affected by current velocity, TOM and pH.
istribution Patterns of the Morphology, Species, and Sex in the Stingray Species Complex of Himantura uarnak, Himantura undulata, and Himantura leoparda in Indonesia Arlyza1, Irma Shita; Solihin, Dedy Duryadi; Soedharma, Dedi
Makara Journal of Science Vol. 17, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The assessment of the morphology and stingray species distribution of the Himantura uarnak species complex covers eight sampling sites in western and eastern Indonesia as many as 113 individuals. The observation of the reticulate pattern was accomplished by directly checking the dorsal side of the stingrays, and growth observations also carried out. The distribution patterns were analyzed using the principal component analysis (PCA), while the growth patterns were evaluated using correlation analyses. The reticulation patterns of the H. uarnak species complex were highly variable, and the distribution patterns based on the morphological parameters, species, and sex produced four groups. The distribution of the length frequency of H. undulata and H. uarnak showed a range of different sizes. The body length of the female in the western region is relatively longer than the male, while in the eastern region the female revealed a range in relative size showing that the male was longer than the female. The length-weight relationships of the stingrays were negative allometric, indicating a b-value of less than 3. The b-values of both the male and female H. undulata were 1.5860 and 0.4380, respectively; while the male and female H. uarnak were 0.2956 and 0.4376, respectively.
Co-Authors A. Zaenal Mustopa Abdul Haris Andry Indrawan Ani Mardiastuti Arlyza1, Irma Shita Auldry Fransje Walukouw Cecep Kusmana D Djokosetiyanto, D Edison Harteman Ekowati Chasanah Enan M. Adiwilaga Harpasis S Sanusi Harpasis S. Sanusi HEDI INDRA JANUAR Hefni Effendi John I. Pariwono KholiPdan, KholiPdan Lilik B Prasetyo Melki Melki Mennofatria Boer MUJIZAT KAWAROE Muliari Muliari NEVIATY PUTRI ZAMANI Niken T.M. Pratiwi Nuri Aslami Nurul Khakhim Rachmaniar Rachmaniar Ratna Siahaan RIDWAN AFFANDI Siregar, Rahmadsyah Deny TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tjahjo Winanto, Tjahjo Vincentius P Siregar Yudi Herdiana Yusli Wardiatno