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All Journal Jurnal Hortikultura JURNAL BIOLOGI INDONESIA Agrin : Jurnal Penelitian Pertanian
Eri Sofiari
Balai Penelitian Tanaman Sayuran, Lembang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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KAJIAN PENDAHULUAN: PERPINDAHAN GEN DARI TANAMAN KENTANG TRANSGENIK KATAHDIN RB KE TANAMAN KENTANG NON TRANSGENIK Ambarwati, A. Dinar; Herman, M.; Purwito, Agus rifcb@indo.net.id; Sofiari, Eri; Aswidinnoor, hajrial
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v7i2.3114

Abstract

ABSTRACTPreliminary study: Gene transfer from transgenic potato Katahdin RB to non transgenicpotato. One of the concerns associated with the release of transgenic crops, is the possibilityof the gene flow from transgenic crops to neighboring crops of the same species or to relatedspecies. In plants, gene flow is a routine process occur through the natural hybridization. Theopportunity for gene flow occur depends principally on two factors, the degree of sexualcompatibility between donor and recipient species, and the physical distance between thetwo. The experiment was conducted to determine whether the gene flow from transgenicpotato Katahdin RB to non transgenic was occurred, based on selection using a 50 mg/lkanamycin, and to estimate gene flow mediated by natural hybridization at different isolationdistances. Preliminary result indicated that a rapid and simple method using MS0 liquid mediawith kanamycin 50 mg/l was effective for screening the seeds. There was a gene flow fromtransgenic potato Katahdin RB to non transgenic, based on a rapid and simple selectionmethod using 50 mg/l of kanamycin as selectable marker. The isolation distance used in thestudy were 0.8, 1.6, 2.4, 3.2, 4.0, 4.8, 5.6, 6.4, 7.2, 8.0, 8.8, 9.6, 10.4, and 11.2 m from the row oftransgenic potato Katahdin RB. The gene flow through natural hybridization at a isolationdistances of (0.8 - 1.6 m), (2.4 ? 4 m), and (4.8 ? 6.4 m) from transgenic to non transgenic plantswere 13.78, 10.92, and 3.82%, respectively. At a distance of 7.2 ? 8 m, the frequency of gene flowwas declined to 0%. The frequency of gene flow from transgenic potatoes to non transgenicpotatoes markedly decreased by increasing the isolation distance, and was negligible at 7.2 m.Key words : natural hybridization, transgenic potato RB, kanamycin selection
ADAPTASI DAN PRODUKSI KLON-KLON KENTANG HARAPAN DI DATARAN MEDIUM MAJALENGKA Handayani, Tri; Kurniawan, Helmi; Sofiari, Eri
Agrin Vol 18, No 1 (2014): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2014.18.1.215

Abstract

Penelitian ini bertujuan untuk menguji daya hasil dan adaptasi klon-klon kentang harapan introduksi di dataran medium. Penelitian dilakukan di Desa Sukasari Kidul, Kecamatan Argapura, Kabupaten Majalengka dengan ketinggian 600 m dpl.  Materi yang ditanam sebanyak 11 klon introduksi dan 2 varietas kentang (Granola dan MB 17). Hasil pengujian menunjukkan semua klon dan varietas yang ditanam memperlihatkan pertumbuhan vegetatif yang baik. Penyakit yang terdeteksi di lapangan antara lain layu bakteri Ralstonia solanacearum, penyakit akibat virus (mosaik), dan kudis. Sedangkan hama yang muncul di lapangan adalah trips dan penggorok daun Liriomyza huidobrensis.  Klon-klon  CIP-391846.5; CIP-395192.1 dan CIP-396311.1 mempunyai produksi ubi di atas 350 gram per tanaman. Klon-klon tersebut mampu beradaptasi di dataran medium Majalengka dan berpotensi untuk dilanjutkan dalam uji daya hasil lanjutan.Kata kunci:  daya hasil, Solanum tuberosum L., suhu tinggi ABSTRACTThe objective of this trial was to evaluate the yield and adaptation of potato clones at medium altitude. The trial was carried out in Sukasari Kidul, Argapura Sub-district, Majalengka, West Java at 600 m asl. The material were planted as many as 13 advance clones and varieties of potatoes. The result showed that all the clones have good vegetative growth. The disease was detected in the field were bacterial wilt (Ralstonia solanacearum), viral diseases (mosaic), and scab, while pests in the field were trips and Liriomyza huidobrensis. Clones CIP-391846.5; CIP-395192.1 and CIP-396311.1 have the tuber production above 350 grams per plant. Those clones are potential to continue in the advanced yield trials. Key words:  yield, Solanum tuberosum L., high temperature
KERAGAAN DERAJAT TOLERANSI BEBERAPA GALUR- GALUR CABAI MERAH (Capsicum annuum L.) TERHADAP PENYAKIT VIRUS KUNING KERITING DI DAERAH ENDEMI Gunaeni, Neni; Kirana, Rinda; Sofiari, Eri
Agrin Vol 18, No 1 (2014): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2014.18.1.212

Abstract

Penyakit virus kuning keriting termasuk dalam grup Gemini sub grup Begomovirus sampai saat ini masih merupakan virus utama yang menyerang cabai dan dapat menurunkan hasil sampai 100 %. Penanaman varietas toleran tidak hanya mengurangi kerugian oleh pathogen tetapi mengurangi biaya penggunaan pestisida dan keamanan lingkungan. Tujuan penelitian ini untuk mengetahui keragaan derajat toleransi sebelas galur cabai asal AVRDC dan tiga varietas komersial di Indonesia terhadap penyakit virus kuning keriting. Penelitian dilaksanakan di daerah endemi penyakit virus kuning keriting Desa Gondowangi, Kecamatan Sawangan, Kabupaten Magelang dan Desa Kersana, Kecamatan Kersana, Kabupaten Brebes pada bulan Juli sampai Desember 2010. Variabel pengamatan dilakukan terhadap insiden dan intensitas gejala virus, penampilan fenotipik dan genotip yang resisten.   Hasil penelitian menunjukkan bahwa : Diperoleh galur cabai yang mempunyai tingkat konsistensi terhadap penyakit kuning keriting yaitu satu galur cabai PP 0537 – 7558 mempunyai tingkat ketahanan resisten/Toleran (tahan/toleran), satu galur  0735 – 56041 – 1  WTG  moderat resisten  (agak tahan), empat galur   0735 – 5623 WTG, Lado, Lembang-1 dan TM – 999 moderat suceptible (agak peka) dan dua galur  0737 – 7651 – B dan 0707 – 7512 – B suceptible (peka). Kata kunci : Capsicum annuum L., keragaan derajat toleransi, penyakit virus kuning keriting ABSTRACTPepper Yellow Leaf Curl Virus disease included in the subgroup Gemini group Begomovirus is still a major viruses that attack peppers and can reduce yield up to 100 %. Using resistance varieties are not only could yield reduce loss but also reduce pesticide used environmental sefty. The Objective   of this study was to determine the concictency level of resistance of eleven lines introduced from AVRDC, and three commercial varieties of Indonesia. The experiment was conducted in endemic areas of Pepper Yellow Leaf Curl Virus disease (Gondowangi Village, Sawangan Distric Magelang and Kersana Village, Brebes) from July to December 2010. Variables observations were made on the incidence and intensity of symptoms of the virus, the appearance of phenotypic and genotypic resistant. The results showed that : Hot pepper line that have acquired a level of consistency to the disease that is one line of Pepper yellow Leaf Curl Virus PP 0537 - 7558 has a resistance level of resistant / tolerant, one lines of 0735 - 56041-1 WTG moderate resistant, four line 0735 - 5623 WTG, Lado, Lembang-1 and TM - 999 suceptible moderate and two lines 0737-7651 - B and 0707-7512 - B suceptible. Key words : (Capsicum annuum L.), diversity level tolerance, Pepper Yellow Leaf Curl Virus
Co-Authors - Kusmana A. Dinar Ambarwati Agus Purwito Ahsol Hasyim Alberta Dinar Ambarwati Ali Asgar E Listanto Edi Lisanto Euis Suryaningsih G A Sopha G A Watimena HAJRIAL ASWIDINNOOR Helmi Kurniawan M Herman M S Sinaga M. Herman Muhamad Herman N M Armini Neni Gunaeni Nunung Nurtika R Murtiningsih Rinda Kirana Rofik Sinung Basuki S T Rahayu Tri Handayani Wiwin Setiawati