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Production of Lactic Acid from Microalgal Biomass Chlorella vulgar ESP-31 as a feedstock using PVA Immobilized Bacteria L. Plantarum 23 Nandini , Atika; Nagarajan , Dilirani; Chang , Jo-Shu
Nusantara Science and Technology Proceedings 1st International Conference Eco-Innovation in Science, Engineering, and Technology
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2020.0525

Abstract

Lactic acid is a valuable industrial chemical that is mostly used in the food and non-food industries such as the pharmaceutical industry. Production of lactic acid from renewable materials can be an alternative method to reduce the high production cost associated with raw material acquirement. In this study, polyvinyl alcohol (PVA) immobilized L. plantarum 23 was used. To obtain high lactic acid productivity and yield from renewable feedstock, the optimal fermentation conditions were determined in both batch and continuous mode. The renewable feedstock used was microalgal biomass Chlorella vulgaris ESP-31. The optimal conditions for this fermentation are pH 5.5, temperature 30°C, PVA particle loading 12.5%, PVA concentration 5.25g cell/L, HRT: 2-4 hrs, carbon source concentration 40 g/L. The feedstock was pretreated and hydrolyzed appropriately and the reducing sugars obtained were used. With microalgal sugars as a feedstock in continuous fermentation mode, the maximum lactic acid productivity of 12.59 g/L/h was achieved, compared to glucose (7.39 g/L/h). The highest yield achieved in this study (0,98 g/g) was obtained when using pure glucose as the feedstock. Considering high productivity as the most important parameter, microalgal biomass seems to be the best feedstock for lactic acid production in continuous fermentation, giving high productivity and yield of 12.59 g/L/h and 0.91 g/g, respectively.
Optimasi Mikroorganisme (LAB) terhadap Pembentukan Asam Laktat dengan Metode Batch Fermentasi Atika Nandini; Silvana Dwi Nuherdiana; Dilirani Nagarajan; Jo Shu Jo Shu- Chang
Akta Kimia Indonesia Vol 6, No 2 (2021)
Publisher : LPPM, Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j25493736.v6i2.7846

Abstract

Pada penelitian ini, produksi asam laktat menggunakan tujuh jenis mikroorganisme (LAB) dengan metode batch fermentasi. Penelitian ini bertujuan untuk mengetahui kinerja LAB secara optimal dalam memproduksi asam laktat sehingga didapatkan yield dan produktivas yang tinggi. Mikroorganisme yang digunakan adalah L. plantarum, L. sakei, Weisella sp. 26, Weisella sp. 28, Weisella sp. 29, Weisella cibaria dan Weisella paramesenteroides. Tahapan penelitian meliputi inkubasi mikroorganisme selama 14 jam kemudian dilanjutkan proses fermentasi selama 24 jam. Analisa glukosa dan asam laktat menggunakan High performance liquid chromatography (HPLC). Produktivitas asam laktat tertinggi 4,46 g/L/jam didapatkan dari hasil fermantasi dengan menggunakan Lactobacillus plantarum.
Minuman “LESER” Lemon Sereh sebagai Minuman Kesehatan Penambah Imunitas Atika Nandini
Madaniya Vol. 2 No. 4 (2021)
Publisher : Pusat Studi Bahasa dan Publikasi Ilmiah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53696/27214834.106

Abstract

Minuman kesehatan yang dapat membantu meningkatkan daya tahan tubuh sangatlah penting untuk dikonsumsi pada era pandemi Covid-19 seperti sekarang ini. Pembuatan minuman “Leser” atau lemon sereh yang dibuat dari bahan-bahan alami sangatlah mudah. Manfaat atau khasiat yang didapat juga sangatlah beragam. Pada kegiatan penyuluhan yang diadakan di Balai RW 04, Perumahan Kosagrha, Kelurahan Medokan Ayu, Kecamatan Rungkut, Kota Surabaya ini memberikan cara pembuatan minuman lemon sereh secara sederhana di mana bahan bakunya yaitu lemon dan sereh banyak sekali ditemukan di pasar tradisional maupun supermarket. Kegiatan penyuluhan yang diberikan kepada warga ini dipaparkan bagaimana proses pembuatan minuman lemon sereh ini yaitu lemon dan sereh dicuci bersih kemudian sereh digeprek atau dihancurkan dan tambahkan air serta perasan jeruk lemon, gula pasir atau gula merah ditambahkan sebagai pemanis. Kemudian rebus hingga mendidih selama 30 menit. Saring ampas rempah dan siap untuk diminum dalam keadaan hangat atau dinginkan terlebih dahulu di dalam kulkas jika ingin dikonsumsi dalam keadaan dingin. Diharapkan dengan adanya kegiatan penyuluhan ini dapat menambah wawasan betapa pentingnya menjaga kesehatan dan meningkatkan imunitas tubuh di era pandemi bagi warga sekitar.
Analysis Effect of Butyrate Addition on Butanol Production by PVA-Immobilized Clostridium acetobutylicum ATCC 824 in Batch Culture Fermentation Atika Nandini; Wei-Yu Chou; Dilirani Nagarajan; Jo-Shu Chang
Nusantara Science and Technology Proceedings 2nd International Conference Eco-Innovation in Science, Engineering, and Technology
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The cultivation of PVA-immobilized Clostridium acetobutylicum ATCC 824 in CRM medium including 60 g/L of glucose as a carbon source was used to produce butanol. The addition of various butyrate concentrations was analyzed to stimulate metabolic pathways toward butanol production. Anaerobic incubation conditions were maintained at a temperature of 37°C for 24-36 h. The A-B-E batch fermentation was performed at the optimum condition as follows: temperature of 37°C, the glucose concentration of 60 g/L, and pH of 4.5 (controlled). The highest butanol production of 14.94 g/L was achieved with butyrare addition of 2.5 g/L, and the highest butanol yield of 0.61 mol butanol/mol glucose was achieved at the addition of 5 g/L. The experimental result showed that butanol production and yield increase when the addition of butyrare is less than 5 g/L. Despite that, with further higher butyrare concentration, butanol production decreases accompanied by decreased glucose consumption. The lowest butanol production of 11.74 g/L was obtained at the addition of 7.5 g/L butyrare.
Brief Overview of Perovskite Oxide: Synthesis and Its Performance as Oxygen Separator from Air Silvana Dwi Nurherdiana; Rachmad Ramadhan Yogaswara; Nove Kartika Erliyanti; Atika Nandini; Mutasim Billah; Sintha Soraya Santi; Hamzah Fansuri; Mohd Hafiz Dzarfan Othman; Yuly Kusumawati; Syafsir Akhlus
Nusantara Science and Technology Proceedings 2nd International Conference Eco-Innovation in Science, Engineering, and Technology
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The aims of this brief overview are providing the knowledge about the physical and chemical properties of perovskite oxides in various synthesis method and the correlation of its performance for separating oxygen from air. Perovskite oxide showed excellent conductor of ion and electron, simultaneously through oxygen lattice in the crystal structure. In the present review, we have presented the detail on the crystal structure and the factor that affect the pseudo-crystal, various synthesis method i.e., solid-state, sol-gel, combustion, and co-precipitation. It has been completed with the discussion of the characteristic and the mechanism of the perovskite to separate oxygen from air
Pelatihan Pembuatan Pupuk Organik Padat Dari Kotoran Sapi Dalam Meningkatkan Produktivitas Tanaman di Desa Tutur, Kabupaten Pasuruan Atika Nandini; Mu’tasim Billah; Revita Arendri Vashti
Sejahtera: Jurnal Inspirasi Mengabdi Untuk Negeri Vol. 2 No. 3 (2023): Juli : Jurnal Inspirasi Mengabdi Untuk Negeri
Publisher : Universitas Maritim AMNI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58192/sejahtera.v2i3.1083

Abstract

Desa tutur merupakan salah satu desa dari Kecamatan Tutur, Kabupaten Pasuruan yang memiliki potensi alam yang melimpah seperti susu sapi, kopi dan bunga krisan. Dalam pembudidayaan bunga krisan diperlukan pupuk yang tepat untuk tanaman agar dapat berkembang dengan baik. Pupuk organik kerap dikenal sebagai pupuk yang berasal dari segala proses sisa makhluk hidup seperti hewan, tumbuhan, dan manusia. Kotoran sapi dari produk susu sapi yang dihasilkan desa Tutur dapat dijadikan pupuk organik karena kaya akan nutrien. Namun pada desa Tutur, belum memanfaatkan limbah kotoran sapi tersebut secara maksimal. Pelatihan pembuatan pupuk organik dari kotoran sapi ini dapat menjadikan peluang baik bagi warga desa Tutur agar membantu pembudidayaan pada bunga krisan dan tanaman lainnya untuk peningkatan produktivitas tanah. Dalam pelatihan ini, warga dijelaskan terkait pupuk organik serta metodologi pembuatan pupuk organik dari kotoran sapi. Hasil pelatihan menunjukan peningkatan pemahaman serta pengetahuan akan pengolahan limbah kotoran sapi sebesar 99%. Hasil tersebut dapat diterapkan oleh warga Tutur kedepannya untuk memanfaatkan kotoran sapi menjadi pupuk organik.
The Effect of Resin and NH4OH Addition in The Making of Ammonium Silica Fertilizer from Geothermal Sludge Kindriari Nurma Wahyusi; Atika Nandini; Lucky Indrati Utami; Siswanto; Isni Utami; Nurul Mardhiyah; Dian Nofita
International Journal of Eco-Innovation in Science and Engineering (IJEISE) Vol. 2 No. 1 (2021): IJEISE
Publisher : International Journal of Eco-Innovation in Science and Engineering (IJEISE)

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Abstract

Geothermal sludge is a waste product from geothermal where it contains SiO2 which can be used as fertilizer. In this study, the making of silica ammonium fertilizer was carried out by taking salicy acid from geothermal sludge by extracting and adding resin which was then modified with NH4OH addition. Extraction was done using 1000 ml of KOH 1 N to dissolve 60 grams of Geothermal Sludge. Resins are added with variations in resin weight, namely, 5, 10, 20, 30, and 40 grams. NH4OH was added with variations in concentrations namely 3, 6, 9, 12 and 15% with a ratio of filtrate and NH4OH solution of 1: 1. The resulting ammonium silica inorganic liquid fertilizer products were analyzed for free silica and ammonia levels. The results of this study indicate that the levels of free silica and ammonia are influenced by the weight of the resin used and the concentration of ammonium hydroxide mixed. From the results of the research, it was found that inorganic silica liquid fertilizer with the highest SiO2 content in ammonium silica liquid fertilizer occurred when adding 20 grams of resin with the addition of NH4OH with a concentration of 15% which was 1,831.87 mg / L while the highest NH3 content in fertilizer liquid ammonium silica occurs when adding 40 grams of resin with the addition of NH4OH with a concentration of 15% which is equal to 252,312.80 mg / L.
PEMANFAATAN LIMBAH NASI AKING PADA PEMBUATAN BIOPLASTIK DENGAN KITOSAN DAN PLASTICIZER SORBITOL Halim, Muhammad Dani; Karangan, Adam; Suprihatin, Suprihatin; Nandini, Atika
Jurnal Teknik Kimia Vol 17, No 2 (2023): Jurnal Teknik Kimia
Publisher : Program Studi Teknik Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33005/jurnal_tekkim.v17i2.3792

Abstract

Bioplastik merupakan salah satu jenis plastik yang dapat terdegradasi oleh aktivitas mikroorganisme di lingkungan. Nasi aking mengandung kadar pati yang tinggi yaitu 70,68% dan berpotensi digunakan sebagai sumber bahan utama pembuatan bioplastik. Penelitian ini bertujuan untuk memperoleh kondisi optimal penambahan kitosan dan sorbitol serta mengetahui pengaruhnya terhadap kualitas produksi bioplastik. Pembuatan bioplastik ini dilakukan menggunakan metode solution casting dengan komposisi tepung nasi aking sebanyak 6 gr yang ditambahkan dengan kitosan dan sorbitol sesuai variasi. Pada penelitian ini, variasi penambahan sorbitol berpengaruh pada persen elongasi. Sedangkan variasi penambahan kitosan berpengaruh pada kuat tarik. Berdasarkan hasil uji, penambahan kitosan 10 gram dan sorbitol 5ml menghasilkan bioplastik dengan kualitas sebagai berikut: nilai kuat tarik sebesar 5,716 Mpa, nilai elongasi sebesar 22,10%, dan persentase biodegradasi sebesar 32,91% dalam waktu 45 hari. Hasil yang diperoleh telah memenuhi SNI No.7188.7 untuk nilai elongasi dan terdegradasi sempurna dalam waktu 45 hari sesuai dengan ASTM 5338. DOI : https://doi.org/10.33005/jurnal_tekkim.v17i2.3792
PENGARUH SUHU DAN WAKTU EKSTRAKSI TERHADAP KANDUNGAN SELULOSA PADA LIMBAH KELAPA SAWIT Nandini, Atika; Wahyusi, Kindriari Nurma; Arinton, Aditya; Maulana, M. Rizky
Jurnal Teknik Kimia Vol 19, No 2 (2025)
Publisher : Program Studi Teknik Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33005/jurnal_tekkim.v19i2.5074

Abstract

Komponen penyusun utama dari limbah kelapa sawit diantaranya adalah selulosa, hemiselulosa dan lignin. Komponen selulosa dapat dimanfaatkan sebagai bahan baku untuk bahan bakar cair (bioethanol, biediesel, dll) maupun sebagai bahan baku untuk pembuatan nanokomposit. Proses pemisahan atau ekstraksi dibutuhkan untuk memisahkan komponen selulosa tersebut dari komponen-komponen penyusun lainnya. Parameter waktu, suhu dan konsentrasi pelarut menjadi faktor-faktor penentu proses ini. Tujuan penelitian ini antara lain mempelajari pengaruh waktu dan suhu terhadap proses ekstraksi selulosa dengan menggunakan metode organosol). Tahapan proses pada penenlitian ini diantaranya adalah proses ekstraksi dengan pelarut etanol 96% dan katalis asam sulfat 5%, kemudian dilanjutkan dengan proses bleaching menggunakan hidrogen peroksida dengan konsentrasi 2%, serta tahapan akhir pengeringan hasil ekstrak selulosa dengan oven pada suhu 60℃. Hasil ekstraksi selulosa dianalisa persentase kandungan selulosa, hemiselulosa, dan lignin dengan metode kuantitatif Chesson. Serbuk selulosa juga dianalisa secara organoleptik berdasarkan standar SNI 0444-2009. Kandungan selulosa tertinggi dengan kadar sebesar 58,71% didapatkan pada kondis suhu 170℃ serta waktu ekstraksi selama 150 menit, sehingga kondisi tersebut ditetapkan sebagai kondisi optimum. Hasil analisa organoleptik menunjukan bahwa serbuk selulosa ini telah sesuai dengan standar SNI 0444 2009.
REDUCTION OF LEAD (Pb) LEVELS IN RED KUPANG (MUSCULISTA SENHAUSIA) USING WULUH STARFRUIT (AVERRHOA BILIMBI) EXTRACT Ningrum, Devy Shinta; Darmawan, Dewangga Haris; Suprihatin, Suprihatin; Nandini, Atika
Jurnal Teknik Kimia Vol 19, No 2 (2025)
Publisher : Program Studi Teknik Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33005/jurnal_tekkim.v19i2.5139

Abstract

Red kupang (Musculista senhausia) is one of the protein-rich fisheries resources that is widely consumed by the population. However, red kupang contains a significant amount of heavy metal. This can be harmful to people if consumed in the long term and not processed properly. The boiling method that has been used to remove heavy metals is not effective, especially for reducing the levels of lead (Pb), which is difficult to dissolve in water. The accumulation of lead (Pb) in red kupang can be reduced by extracting it using citric acid, a chelating agent. This study aims to determine the effect of differences in citric acid concentration (0.5%v/v, 1%v/v, 1.5%v/v, 2%v/v, and 2.5%v/v) from starfruit juice solvent and stirring speed (150 rpm, 180 rpm, 210 rpm, 240 rpm, and 270 rpm) in the solid-liquid extraction method (leaching) on lead (Pb) levels in red kupang. Analysis of lead (Pb) levels was carried out using AAS. The analysis results showed that the levels of lead (Pb) tended to decrease as the stirring speed and citric acid concentration increased. The best results were obtained at a citric acid concentration of 2.5%v/v and a stirring speed of 210 rpm, which resulted in a residual lead (Pb) levels of 0.251 mg/kg. The results are in accordance with SNI 7387:2009, which states that the limit for lead metal contamination in food is 1.5 mg/kg.