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Production of Lactic Acid from Microalgal Biomass Chlorella vulgar ESP-31 as a feedstock using PVA Immobilized Bacteria L. Plantarum 23 Nandini , Atika; Nagarajan , Dilirani; Chang , Jo-Shu
Nusantara Science and Technology Proceedings 1st International Conference Eco-Innovation in Science, Engineering, and Technology
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2020.0525

Abstract

Lactic acid is a valuable industrial chemical that is mostly used in the food and non-food industries such as the pharmaceutical industry. Production of lactic acid from renewable materials can be an alternative method to reduce the high production cost associated with raw material acquirement. In this study, polyvinyl alcohol (PVA) immobilized L. plantarum 23 was used. To obtain high lactic acid productivity and yield from renewable feedstock, the optimal fermentation conditions were determined in both batch and continuous mode. The renewable feedstock used was microalgal biomass Chlorella vulgaris ESP-31. The optimal conditions for this fermentation are pH 5.5, temperature 30°C, PVA particle loading 12.5%, PVA concentration 5.25g cell/L, HRT: 2-4 hrs, carbon source concentration 40 g/L. The feedstock was pretreated and hydrolyzed appropriately and the reducing sugars obtained were used. With microalgal sugars as a feedstock in continuous fermentation mode, the maximum lactic acid productivity of 12.59 g/L/h was achieved, compared to glucose (7.39 g/L/h). The highest yield achieved in this study (0,98 g/g) was obtained when using pure glucose as the feedstock. Considering high productivity as the most important parameter, microalgal biomass seems to be the best feedstock for lactic acid production in continuous fermentation, giving high productivity and yield of 12.59 g/L/h and 0.91 g/g, respectively.
Optimasi Mikroorganisme (LAB) terhadap Pembentukan Asam Laktat dengan Metode Batch Fermentasi Atika Nandini; Silvana Dwi Nuherdiana; Dilirani Nagarajan; Jo Shu Jo Shu- Chang
Akta Kimia Indonesia Vol 6, No 2 (2021)
Publisher : LPPM, Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j25493736.v6i2.7846

Abstract

Pada penelitian ini, produksi asam laktat menggunakan tujuh jenis mikroorganisme (LAB) dengan metode batch fermentasi. Penelitian ini bertujuan untuk mengetahui kinerja LAB secara optimal dalam memproduksi asam laktat sehingga didapatkan yield dan produktivas yang tinggi. Mikroorganisme yang digunakan adalah L. plantarum, L. sakei, Weisella sp. 26, Weisella sp. 28, Weisella sp. 29, Weisella cibaria dan Weisella paramesenteroides. Tahapan penelitian meliputi inkubasi mikroorganisme selama 14 jam kemudian dilanjutkan proses fermentasi selama 24 jam. Analisa glukosa dan asam laktat menggunakan High performance liquid chromatography (HPLC). Produktivitas asam laktat tertinggi 4,46 g/L/jam didapatkan dari hasil fermantasi dengan menggunakan Lactobacillus plantarum.
Minuman “LESER” Lemon Sereh sebagai Minuman Kesehatan Penambah Imunitas Atika Nandini
Madaniya Vol. 2 No. 4 (2021)
Publisher : Pusat Studi Bahasa dan Publikasi Ilmiah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53696/27214834.106

Abstract

Minuman kesehatan yang dapat membantu meningkatkan daya tahan tubuh sangatlah penting untuk dikonsumsi pada era pandemi Covid-19 seperti sekarang ini. Pembuatan minuman “Leser” atau lemon sereh yang dibuat dari bahan-bahan alami sangatlah mudah. Manfaat atau khasiat yang didapat juga sangatlah beragam. Pada kegiatan penyuluhan yang diadakan di Balai RW 04, Perumahan Kosagrha, Kelurahan Medokan Ayu, Kecamatan Rungkut, Kota Surabaya ini memberikan cara pembuatan minuman lemon sereh secara sederhana di mana bahan bakunya yaitu lemon dan sereh banyak sekali ditemukan di pasar tradisional maupun supermarket. Kegiatan penyuluhan yang diberikan kepada warga ini dipaparkan bagaimana proses pembuatan minuman lemon sereh ini yaitu lemon dan sereh dicuci bersih kemudian sereh digeprek atau dihancurkan dan tambahkan air serta perasan jeruk lemon, gula pasir atau gula merah ditambahkan sebagai pemanis. Kemudian rebus hingga mendidih selama 30 menit. Saring ampas rempah dan siap untuk diminum dalam keadaan hangat atau dinginkan terlebih dahulu di dalam kulkas jika ingin dikonsumsi dalam keadaan dingin. Diharapkan dengan adanya kegiatan penyuluhan ini dapat menambah wawasan betapa pentingnya menjaga kesehatan dan meningkatkan imunitas tubuh di era pandemi bagi warga sekitar.
Analysis Effect of Butyrate Addition on Butanol Production by PVA-Immobilized Clostridium acetobutylicum ATCC 824 in Batch Culture Fermentation Atika Nandini; Wei-Yu Chou; Dilirani Nagarajan; Jo-Shu Chang
Nusantara Science and Technology Proceedings 2nd International Conference Eco-Innovation in Science, Engineering, and Technology
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The cultivation of PVA-immobilized Clostridium acetobutylicum ATCC 824 in CRM medium including 60 g/L of glucose as a carbon source was used to produce butanol. The addition of various butyrate concentrations was analyzed to stimulate metabolic pathways toward butanol production. Anaerobic incubation conditions were maintained at a temperature of 37°C for 24-36 h. The A-B-E batch fermentation was performed at the optimum condition as follows: temperature of 37°C, the glucose concentration of 60 g/L, and pH of 4.5 (controlled). The highest butanol production of 14.94 g/L was achieved with butyrare addition of 2.5 g/L, and the highest butanol yield of 0.61 mol butanol/mol glucose was achieved at the addition of 5 g/L. The experimental result showed that butanol production and yield increase when the addition of butyrare is less than 5 g/L. Despite that, with further higher butyrare concentration, butanol production decreases accompanied by decreased glucose consumption. The lowest butanol production of 11.74 g/L was obtained at the addition of 7.5 g/L butyrare.
Brief Overview of Perovskite Oxide: Synthesis and Its Performance as Oxygen Separator from Air Silvana Dwi Nurherdiana; Rachmad Ramadhan Yogaswara; Nove Kartika Erliyanti; Atika Nandini; Mutasim Billah; Sintha Soraya Santi; Hamzah Fansuri; Mohd Hafiz Dzarfan Othman; Yuly Kusumawati; Syafsir Akhlus
Nusantara Science and Technology Proceedings 2nd International Conference Eco-Innovation in Science, Engineering, and Technology
Publisher : Future Science

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Abstract

The aims of this brief overview are providing the knowledge about the physical and chemical properties of perovskite oxides in various synthesis method and the correlation of its performance for separating oxygen from air. Perovskite oxide showed excellent conductor of ion and electron, simultaneously through oxygen lattice in the crystal structure. In the present review, we have presented the detail on the crystal structure and the factor that affect the pseudo-crystal, various synthesis method i.e., solid-state, sol-gel, combustion, and co-precipitation. It has been completed with the discussion of the characteristic and the mechanism of the perovskite to separate oxygen from air
The Effect of Resin and NH4OH Addition in The Making of Ammonium Silica Fertilizer from Geothermal Sludge Kindriari Nurma Wahyusi; Atika Nandini; Lucky Indrati Utami; Siswanto; Isni Utami; Nurul Mardhiyah; Dian Nofita
International Journal of Eco-Innovation in Science and Engineering (IJEISE) Vol. 2 No. 1 (2021): IJEISE
Publisher : International Journal of Eco-Innovation in Science and Engineering (IJEISE)

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Abstract

Geothermal sludge is a waste product from geothermal where it contains SiO2 which can be used as fertilizer. In this study, the making of silica ammonium fertilizer was carried out by taking salicy acid from geothermal sludge by extracting and adding resin which was then modified with NH4OH addition. Extraction was done using 1000 ml of KOH 1 N to dissolve 60 grams of Geothermal Sludge. Resins are added with variations in resin weight, namely, 5, 10, 20, 30, and 40 grams. NH4OH was added with variations in concentrations namely 3, 6, 9, 12 and 15% with a ratio of filtrate and NH4OH solution of 1: 1. The resulting ammonium silica inorganic liquid fertilizer products were analyzed for free silica and ammonia levels. The results of this study indicate that the levels of free silica and ammonia are influenced by the weight of the resin used and the concentration of ammonium hydroxide mixed. From the results of the research, it was found that inorganic silica liquid fertilizer with the highest SiO2 content in ammonium silica liquid fertilizer occurred when adding 20 grams of resin with the addition of NH4OH with a concentration of 15% which was 1,831.87 mg / L while the highest NH3 content in fertilizer liquid ammonium silica occurs when adding 40 grams of resin with the addition of NH4OH with a concentration of 15% which is equal to 252,312.80 mg / L.