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All Journal JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA SIMBIOSIS
SANG KETUT SUDIRGA
Lab. Struktur dan Perkembangan Hewan, **Lab. Fisiologi Tumbuhan Jurusan Biologi FMIPA Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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ISOLASI DAN UJI PATOGENITAS JAMUR Colletotrichum spp. PENYEBAB PENYAKIT ANTRAKNOSA PADA BUAH TOMAT (Solanum lycopersicum L.) : ISOLATION AND PATHOGENITY TESTING OF FUNGI Colletotrichum spp. CAUSES OF ANTHRACNOSE DISEASE ON TOMATOES (Solanum lycopersicum L.) Laila Maghfirotun Ni’mah; Sudirga, Sang Ketut; Darmadi, Anak Agung Ketut
SIMBIOSIS Simbiosis Volume 13 No. 2 2025
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2025.v13.i02.p02

Abstract

Tomatoes (Solanum lycopersicum L.) is a fruit with important economic value and is a leading horticultural commodity in Indonesia. Anthracnose is a disease that can infect tomato plants. From previous research, several types of isolates of Colletotrichum spp. Infecting different hosts can cause different disease severity. The aim of this research was to identify the Colletotrichum fungus that causes anthracnose on tomatoes from several gardens in Bali to test its level of pathogenicity. Isolation of the fungus Colletotrichum spp. using the direct planting method. The pathogenicity test uses the disease severity (KP) scoring method using the formula. Identification and morphological characterization of isolates of Colletotrichum spp. using the identification book "Illustrated Genera of Imperfect Fungi". The parameters observed were colony shape, hyphae and conidia characters and disease severity percentage (KP) on tomatoes. This research used a completely randomized design (CRD) and the data were analyzed statistically using the ANOVA (Analysis of Variance) test with SPSS version 26 software. The results of the identification of macroscopic and microscopic characters from PS, BS and SW isolates showed differences in colony, hyphae and conidia characters. The pathogenicity test results of the PS, BS, and SW isolates showed that the SW isolate was the most adaptive and pathogenic isolate. Keywords: Identification, isolate, hyphae, spores, disease severity
UJI DAYA HAMBAT JAMUR Fusarium spp. MENGGUNAKAN EKSTRAK DAUN TREMBESI : JUDUL INGGRIS FUNGI INHIBITORY Test Fusarium spp. USING LEAF EXTRACT OF TTREMBESI Purnamaningsih, Ni Putu; Sudirga, Sang Ketut; Parwanayomi, Ni Made Susun
SIMBIOSIS Simbiosis Volume 13 No. 2 2025
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2025.v13.i02.p09

Abstract

ABSTRACT Production of large red chilies has decreased due to wilt disease. Continuous use of synthetic fungicides will have a negative impact on the ecosystem and health. Vegetable pesticides are one of the environmentally friendly pesticides, because they are easily decomposed in nature. Trembesi (Samanea saman (Jacq.) Merr) is one of the plants that has the potential to be used as a botanical pesticide. This research aims to determine the ability of trembesi leaf extract to inhibit the growth of Fusarium spp. Diffusion well method to determine the inhibitory power of trembesi leaf extract with optimal concentration in vitro. One-way ANOVA test was used to analyze research data. If there is a significant difference (P <0.05), then proceed with the Duncan post-hoc test. The results showed that the control group and the extract treatment group had significant differences (P <0.05). The largest inhibitory zone is a concentration of 20% with an inhibitory zone diameter of 21.37 mm Kata kunci: Extract, inhibitory, antifungal, Fusarium
Co-Authors Anak Agung Ketut Darmadi Dwi Ariani Yulihastuti I Ketut Ginantra Laila Maghfirotun Ni’mah Parwanayomi, Ni Made Susun Purnama Ningsih Rozi, Muhammad Fathur Wijaya, I Made Saka