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All Journal Jurnal Riset Kesehatan Poltekkes Depkes Bandung Jurnal Kesehatan Siliwangi
Wiwin Wiryanti
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Biochemistry, Genetics & Molecular Biology Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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VALIDASI METODE GOD-PAP PADA PEMERIKSAAN GLUKOSA DARAH DENGAN PEMAKAIAN SETENGAH VOLUME REAGEN DAN SAMPEL Ika Nurhayati; Ani Riyani; Nani Kurnaeni; Wiwin Wiryanti; Sonny Feisal Rinaldi
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 11 No 1 (2019): Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (254.444 KB) | DOI: 10.34011/juriskesbdg.v11i1.792

Abstract

Pemakaian setengah volume reagen dan sampel pada pemeriksaan glukosa darah metode GOD-PAP diketahui tidak memiliki perbedaan terhadap nilai simpangan baku dan koefisien korelasi dibandingkan dengan metode standarnya. Metode GOD-PAP setengah volume reagen dan sampel ini lebih efisien dalam segi ekonomi namun belum tervalidasi. Penelitian ini bertujuan untuk mengetahui kinerja metode GOD-PAP setengah volume reagen dan sampel terhadap pemeriksaan glukosa darah. Jenis penelitian ini adalah deskriptif analitik dengan melakukan pengumpulan dan analisis data dari hasil pengujian terhadap delapan parameter validasi yaitu presisi, akurasi, linearitas, reportable range, LoD, LoQ, recovery, dan interferen. Sampel yang digunakan berupa serum kontrol komersial normal (Level 1) dan patologis (Level 2). Data yang diperoleh diolah dengan uji statistik deskriptif menggunakan Microsoft Excel. Hasil penelitian menunjukkan nilai presisi berupa CV Level 1= 1,66%, Level 2= 1,37% (CV<0,33 TEa); akurasi berupa TE Level 1= 3,84%, Level 2= 3,76% (TE<TEa); linearitas dengan nilai r=0,997 (r>0,990); reportable range dengan rentang nilai ≥ rentang nilai normal parameter; LoD = 7,51 mg/dL; LoQ = 20,54 mg/dL (lebih kecil dari batas nilai normal terendah); %Recovery = 99% (berada dalam rentang 95-105%); dan uji interferen menunjukkan hemoglobin mulai mengganggu pemeriksaan dengan %selisih>TEa pada konsentrasi 600 mg/dL. Berdasarkan hasil tersebut dapat disimpulkan bahwa semua parameter uji validasi memenuhi kriteria penerimaan sehingga metode GOD-PAP dengan pemakaian setengah volume reagen dan sampel diterima kinerjanya sebagai prosedur tervalidasi pada pemeriksaan glukosa darah.
Comparison of Color Quality in Tissue Preparations using Methyl Ester Sulfonate And Xylol as a Deparafinization Agent Rahmah, Syafa Aulia; Adang Durachim; Wiwin Wiryanti; Asep Iin Nur Indra
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Deparaffinization is the process of decaying the remnants of paraffin in tissue preparations. Deparaffinization aims to make the dye that will be given to the preparation fully absorbed so that the preparation can be colored properly. This deparaffinization process uses xylol solution in its immersion. Methyl Ester Sulfonate (MES) is one of the vegetable oil-based anionic surfactants that can dissolve in water and has a long carbon chain that is soluble in oil and vaseline. The purpose of this study was to determine whether Methyl Ester Sulfonate can be used as a deparaffinizing agent for tissue preparations. The sample in this study was appendiceal tissue as many as 30 units, each unit of tissue made into 2 tissue preparations so that it became 60 tissue preparations, namely 30 tissues using xylol as a deparaffinizing agent and 30 tissues using methyl ester sulfonate. The parameters of this study were the clarity of the color of the nucleus and cytoplasm, the uniformity of cell color, and the contrast of the color of the nucleus and cytoplasm qualitatively and quantitatively. The statistical test used was Independent T-Test test if the data were normally distributed and Mann Whitney test if the data were not normally distributed. Based on the Independent T-Test test, the Sig value was obtained. 0.951 and 0.102, both of which are >0.05, meaning that there is no significant difference between the use of xylol and 10% methyl ester sulfonate as a deparaffinizing agent.
Co-Authors Adang Durachim Ani Riyani Asep Iin Nur Indra Ika Nurhayati Nani Kurnaeni Rahmah, Syafa Aulia Sonny Feisal Rinaldi