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All Journal Jurnal Sains Dasar Jurnal Penelitian Pendidikan IPA (JPPIPA) Kingdom (The Journal of Biological Studies)
Lili Sugiyarto
Yogyakarta State University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Materials Science & Nanotechnology Physics Public Health

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APPLICATON OF MYCORRIZA ON PLANTING MEDIA OF TWO TOMATO VARIETIES TO INCREASEVEGETABLE PRODUCTIVITY IN DROUGHT CONDITION Kuswandi, Paramita Cahyaningrum; Sugiyarto, Lili
Jurnal Sains Dasar Vol 4, No 1 (2015): April 2015
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (530.868 KB) | DOI: 10.21831/jsd.v4i1.8432

Abstract

A method used for the development of dry areas/marginal lands is the improvement of soil structure and addition to the media to support the growth and development of crops. Tomatoes have the potential to be developed in marginal lands due to its high nutrition, high in demand and easy to be cultivated. One of the method used to improve planting media is the addition of microorganism such as mycorrhiza which can help the absorbtion of water and nutrition for plants. The interval of irrigation is used as a simulation of drought. This research aim was to observe the effect of mycorrhiza in the soil on the growth and development of tomato with several treatments of irrigation. The method used was the addition of 4 g of mycorrhiza  per polybag (size 30x30 cm2), using Complete Randomized Design. There were 6 combinations of treatments. The treatments were : 3 interval of irrigation (every day, every 7 days and every 14 days), and 2 treatments of mycorrhiza (0 g and 4 g). There were 3 repetition for each combination of treatments. The results showed that the addition of mycorrhiza can increase significantly plant fresh and dry weight and also root length. The difference in plant height, number of branches, number of leaves, plant growth rate and percentage of infection were caused by the difference in irrigation interval. The difference in the varieties used also contribute to a difference in the percentage of infection. Further research must be made on the effect of mycorrhiza with addition of inorganic fertilizer to increase the growth and development of tomato plants in water stressed condition. Keywords:   mycorriza, tomato, draught simulation
KEANEKARAGAMAN ANGGREK ALAM DAN KEBERADAAN MIKORIZA ANGGREK DI DUSUN TURGO PAKEM, SLEMAN YOGYAKARTA Sugiyarto, Lili; Umniyatie, Siti; Henuhili, Victoria
Jurnal Sains Dasar Vol 5, No 2 (2016): October 2016
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1069.878 KB) | DOI: 10.21831/jsd.v5i2.13715

Abstract

Penelitian ini bertujuan untuk melihat keanekaragaman, mikoriza simbion, dan keberadaan mikoriza di akar anggrek alam di Dusun Turgo Pakem Yogyakarta. Metode yang digunakan dengan mengidentifikasi jenis anggrek, mengisolasi dan mengkarakterisasi fungi mikoriza adalah media PDA (potato dextrose agar) yang dilanjutkan dengan identifikasi setiap fungi yang diperoleh. Pengukuran faktor lingkungan abiotik meliputi faktor edafik yaitu kelembaban, pH, suhu tanah, dan mikroklimat yaitu suhu udara, kelembaban udara, dan intensitas cahaya. Infeksi mikoriza diamati dengan mengamati keberadaan fungi pada jaringan akar. Hasil pengamatan menunjukkan adanya 8 spesies anggrek alam yaitu : Zeuxine petakinensis, endrobium sagittatum, Bulbophyllum chaetonium, Dendrobium mutabile, Eria retusa, Eria oblitterata, Pholidota carnea, Rhomboda velutina. Beberapa anggrek spesies di hutan tersebut dalam keadaan dorman sehingga tidak dapat diidentifikasi.  Keberadaan mikoriza dari hasil pengamatan mikroskopis menunjukkan bahwa pada umumnya akar anggrek spesies yang ditemui berasosiasi simbiose dengan mikoriza yang masing-masing menunjukkan jenis fungi yang berbeda. Fungi yang dapat didentifikasi diantaranya termasuk dalam genus Rhizoctonia, Glomus, Chaetonium, Scopulariopsis dan TrichodermaKata kunci: spesies anggrek, mikoriza, fungi anggrek Abstract This research aims to identify the diversity of orchids and to find the existence of mycorrrhizal symbiont in wild orchid roots in Turgo village, Pakem Sleman.                 The samples of orchids and orchid roots were done by taking samples in Puspa Forest of Turgo Village, Pakem Sleman Yogyakarta. This research were exploration research on existing orchids’ diversity. The methods that have been used to identify the existing orchid species in the forest, isolation, and characterization mycorrhizal fungi is PDA (potato dextrose agar). Measurement of environmental factors for including edhapic factors were humidity, pH, soil temperature, and microclimate factors were air temperature, air humidity, and light intensity. The mycorrhizal infection was done by observing of mycorrhizal fungi existence.            The results of the research are retrieved 8 species of orchids are Zeuxine petakinensis, Dendrobium sagittatum, Bulbophyllum chaetonium, Dendrobium mutabile, Eria retusa, Eria oblitterata, Pholidota carnea, Rhomboda velutina. Some orchids in the forest are dormant, therefore it couldn’t be identified. The mycorrhizal symbiosis from microscopic characterization had been known as different on each mychorriza. The mycorrhizal fungi that have been identified were genus of Rhizoctonia, Glomus, Chaetonium, Scopulariopsis and Trichoderma. Keywords: orchid species, mychorrhiza, orchid fungi
PENGARUH PEMBERIAN MIKORIZA TERHADAP KUALITAS BUAH TOMAT (Lycopersicum esculentum Mill. var. Servo) DALAM BEBERAPA VARIASI KONSENTRASI CEKAMAN SALINITAS Indrawati, Agnes Eri; Sugiyarto, Lili
Kingdom: The Journal of Biological Studies Vol 9, No 1 (2023): Kingdom: The Journal of Biological Studies
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/kingdom.v9i1.18287

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian mikoriza terhadap kualitas buah tanaman tomat (Lycopersicum esculentum Mill. var. Servo) pada beberapa konsentrasi salinitas. Lokasi penelitian bertempat di Green House Biologi, FMIPA, UNY pada bulan Desember 2020 – Mei 2021. Penelitian ini merupakan penelitian eksperimen dengan desain Rancangan Acak Lengkap (RAL) dua faktor yaitu mikoriza dan salinitas. Perlakuan mikoriza pada penelitian ini adalah tidak diberi mikoriza dan diberi mikoriza. Konsentrasi salinitas yang diberikan adalah 0 ppm, 550 ppm, 1100 ppm, dan 2750 ppm. Objek penelitian yang digunakan adalah tanaman tomat varietas Servo F1. Data kuantitatif dianalisis menggunakan uji Two-Way ANOVA (Univariate)dilanjutkan dengan uji DuncanMultiple Range Test (DMRT). Hasil penelitian menunjukkan bahwa perlakuan mikoriza serta perlakuan salinitas tidak berpengaruh nyata terhadap parameter jumlah kuncup, jumlah buah, dan ketebalan daging buah, namun berpengaruh nyata terhadap kemanisan buah. Perlakuan salinitas berpengaruh nyata terhadap berat buah dan diameter buah.Kata kunci: Cekaman salinitas, kualitas buah, Lycopersicum esculentum Mill. var. Servo, mikoriza This research aims to observe the effect of giving mycorrhiza on the fruit quality of tomato plants (Lycopersicum esculentum Mill. var. Servo) at several salinity concentrations. The research location was held at Green House Biology, FMIPA, UNY in December 2020 – May 2021. This research was an experimental study using complete randomized design (CRD) with two factors, namely mycorrhiza and salinity. Mycorrhizal treatment in this study were without mycorrhiza and with the addition of  mycorrhiza. The salinity concentrations treatment were 0 ppm, 550 ppm, 1100 ppm, and 2750 ppm. The object of this research was Servo F1 tomato plant. Quantitative data were analyzed using Two-Way ANOVA (Univariate) test followed by Duncan Multiple Range Test (DMRT). The results showed that mycorrhizal and salinity treatments had no significant effect on the parameters of buds number, fruitnumber, and thickness of fruit flesh, but had significant effect on fruit sweetness. Salinity treatment had significant effect on fruit weight and fruit diameter.Keywords: fruit quality, Lycopersicum esculentum Mill. var. Servo, mycorrhizae, salinity stress
Antidiabetic Molecular Mechanisms of Active Compounds from Several Orchids Yulianti, Evy; Mercuriani, Ixora Sartika; Sugiyarto, Lili; Huang, Tzou-Chi
Jurnal Penelitian Pendidikan IPA Vol 9 No 8 (2023): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i8.3940

Abstract

Hyperglycemia condition that leads to diabetes causes various complications. Various active compounds from plants have been studied for their antidiabetic abilities. One of them is the orchid plant. Besides being used as decoration, orchids contain several active compounds that have been proven to be used in medicine, including diabetes. This article discusses the antidiabetic mechanism of several active compounds obtained from orchids. Publication regarding orchid plant for diabetes were found in databases such as PubMed, Google Scholar, Wiley, Science Direct, Medline, Scopus, and Springer. Keywords used in this study were “orchid”, “diabetes”, “hyperglycemia”, “compound” and “herbal”. Out of the 447 collected articles (published in the period between 2011 and 2022), 416 were excluded due to non-relevant studies. There were 31 eligible studies included in this article. In conclusion, the antidiabetic mechanisms of the orchid extracts were as antioxidant, anti-inflammatory and anti-glycation agents, increasing insulin action, influencing lipid metabolism, and inhibiting α‑amylase and α‑glucosidase activity.  
Characterization and Identification of Indol Acetic Acid Phytohormone-producing Bacteria from Corn Root (Zea mays L.) Putri Maharani, Rendha; Aeni Ariyanti, Nur; Aloysius, Suyitno; Sugiyarto, Lili
Jurnal Sains Dasar Vol. 14 No. 2 (2025): October 2025
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Rhizobacteria are endophytic bacteria that inhabit plant tissues and the rhizosphere, producing secondary metabolites that support plant growth. This study is an exploratory descriptive investigation aimed at characterizing and identifying IAA- and phytohormone-producing endophytic rhizobacteria isolated from the roots of corn plants (Zea mays L.). The rhizobacteria isolates producing IAA phytohormones were obtained by culturing bacteria in Yeast Peptone Broth (YPB) medium supplemented with L-tryptophan. Bacterial cultures (1 mL) were treated with Salkowski reagent, and absorbance was measured spectrophotometrically at a wavelength of 530 nm. From 14 isolates, five were identified as producing the highest levels of indole-3-acetic acid (IAA), ranging from 17 to 50 ppm. Identification was conducted using the profile matching method to determine the genus of each rhizobacterium by comparing its characteristics with reference genera. Based on 62 tested traits, the five isolates exhibited diverse features, including Gram-positive and Gram-negative bacteria, coccus-shaped morphology, circular and irregular colony configurations, lobate and undulate margins, convex, umbonate, and raised elevations, and colony colors ranging from white to yellowish, along with varied physiological (biochemical) test results. The identification results revealed bacteria with a similarity index of ≥70%, suggesting proximity to the genus Micrococcus (77.4%, isolate N), Rhizobium (80.6%, isolate D), and Shewanella (80.6%, isolate B; 87.1%, isolate V; and 91.9%, isolate E). This research provides an important contribution to the development of biofertilizers aimed at enhancing the growth and productivity of corn plants sustainably.
Co-Authors Aeni Ariyanti, Nur Evy Yulianti Huang, Tzou-Chi Indrawati, Agnes Eri Ixora Sartika Mercuriani Paramita Cahyaningrum Kuswandi Putri Maharani, Rendha Siti Umniyatie Suyitno Aloysius Victoria Henuhili